Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,401,6070CT55.0% 133.4 / inf 462T312T (ACC→ACT) hflXGTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator
Reads supporting (aligned to +/- strand):  ref base C (93/114);  new base T (128/125);  total (221/241)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 469 total aligned reads displayed.

TGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCC  >  NC_000913/4401459‑4401757
                                                                                                                                                    |                                                                                                                                                      
tggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAActct                                                                                                                                                      >  6:346431/1‑151 (MQ=255)
 ggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTctg                                                                                                                                                     <  8:686398/151‑1 (MQ=255)
   tggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACAGCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCGCACGAGATCCCAACTctgcg                                                                                                                                                   >  7:1582917/1‑150 (MQ=255)
         cATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGAGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGtgat                                                                                                                                             <  4:837497/151‑1 (MQ=255)
            ttAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGtgatga                                                                                                                                           >  8:1657296/1‑150 (MQ=255)
            ttAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGtgatga                                                                                                                                           >  5:466784/1‑150 (MQ=255)
             tAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGaa                                                                                                                                          <  1:356598/150‑1 (MQ=255)
             tAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAAc                                                                                                                                         >  5:765446/1‑151 (MQ=255)
             tAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAAc                                                                                                                                         >  3:93777/1‑151 (MQ=255)
              aaaGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCACCCCTGCTGGTGATGAAc                                                                                                                                         >  4:930249/1‑150 (MQ=255)
                 gCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGa                                                                                                                                     <  5:94345/151‑1 (MQ=255)
                  ccACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACa                                                                                                                                        >  4:517580/1‑147 (MQ=255)
                  ccACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACa                                                                                                                                        >  5:1683998/1‑147 (MQ=255)
                   cACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATc                                                                                                                                   <  4:1081422/151‑1 (MQ=255)
                   cACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATc                                                                                                                                   <  2:270283/151‑1 (MQ=255)
                    aCGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCg                                                                                                                                  <  6:60265/151‑1 (MQ=255)
                      gTTACAAGAGACGCGGCAAGCCACATTACTGCTGGACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGat                                                                                                                                <  4:1315560/151‑1 (MQ=255)
                        tACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGata                                                                                                                               <  3:435037/150‑1 (MQ=255)
                        tACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGata                                                                                                                               <  4:408282/150‑1 (MQ=255)
                         aCAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGCTCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATg                                                                                                                             >  8:739065/1‑151 (MQ=255)
                          cAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGc                                                                                                                            >  3:641456/1‑151 (MQ=255)
                            agagACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTg                                                                                                                          >  3:296435/1‑151 (MQ=255)
                                aCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAg                                                                                                                      <  5:664481/151‑1 (MQ=255)
                                aCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAg                                                                                                                      <  7:217114/151‑1 (MQ=255)
                                 cgcgGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGa                                                                                                                        >  7:1059103/1‑148 (MQ=255)
                                     gCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTc                                                                                                                 >  3:79540/1‑151 (MQ=255)
                                     gCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTc                                                                                                                 >  6:1259335/1‑151 (MQ=255)
                                     gCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTc                                                                                                                 <  1:889956/151‑1 (MQ=255)
                                      cAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCg                                                                                                                >  3:12765/1‑151 (MQ=255)
                                       aaGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCg                                                                                                                >  8:1424556/1‑150 (MQ=255)
                                       aaGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCg                                                                                                                >  5:1521773/1‑150 (MQ=255)
                                         gCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCATGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAAc                                                                                                             <  1:437682/151‑1 (MQ=255)
                                            acaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgc                                                                                                          <  6:1180947/151‑1 (MQ=255)
                                            acaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgc                                                                                                          >  8:862620/1‑151 (MQ=255)
                                             caTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgcg                                                                                                         <  5:131275/151‑1 (MQ=255)
                                             caTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgcg                                                                                                         >  8:265723/1‑151 (MQ=255)
                                             caTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgcg                                                                                                         >  5:740027/1‑151 (MQ=255)
                                              aTTACTGCTGGACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGAGCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATTCTGGAAGAGTTCGAAGCGCGt                                                                                                        >  7:1224666/1‑151 (MQ=255)
                                              aTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGt                                                                                                        >  8:116876/1‑151 (MQ=255)
                                                tACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTa                                                                                                       <  7:669610/150‑1 (MQ=255)
                                                 aCTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAtt                                                                                                     >  7:452085/1‑151 (MQ=255)
                                                  ctgctgCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCCATATGCTGGAAGATTTCGAACCGCGTATTg                                                                                                    >  2:1229465/1‑151 (MQ=255)
                                                    gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGa                                                                                                   <  5:223293/150‑1 (MQ=255)
                                                       gcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATc                                                                                                 <  1:713493/149‑1 (MQ=255)
                                                        cACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCggg                                                                                              >  6:1479819/1‑151 (MQ=255)
                                                              aTTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGa                                                                                          >  3:902146/1‑149 (MQ=255)
                                                                tGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAag                                                                                        >  7:1642692/1‑149 (MQ=255)
                                                                 gATGCGGCGGATGTGCGTTTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAagag                                                                                      <  8:514057/150‑1 (MQ=255)
                                                                 gATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAagag                                                                                      <  3:1176295/150‑1 (MQ=255)
                                                                 gATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAag                                                                                        >  6:1699770/1‑148 (MQ=255)
                                                                  aTGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGaa                                                                                    <  1:261/151‑1 (MQ=255)
                                                                  aTGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACAAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGaa                                                                                    <  6:397615/151‑1 (MQ=255)
                                                                    gcggctGATTTTCGTGTACAAGAAAAGATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTTCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAAc                                                                                   <  7:1155583/150‑1 (MQ=255)
                                                                      ggcggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACaaa                                                                                <  1:811413/151‑1 (MQ=255)
                                                                       gcggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaac                                                                               <  6:454145/151‑1 (MQ=255)
                                                                        cggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaacc                                                                              <  5:318414/151‑1 (MQ=255)
                                                                           aTGTGCGTGTACAAGAAAACATCGAAGCGGTTAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaa                                                                           <  2:167461/151‑1 (MQ=255)
                                                                           aTGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaa                                                                           <  4:286844/151‑1 (MQ=255)
                                                                           aTGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaa                                                                           <  4:1497201/151‑1 (MQ=255)
                                                                           aTGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccga                                                                            >  7:1419883/1‑150 (MQ=255)
                                                                             gtgCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaacc                                                                         <  7:304888/151‑1 (MQ=255)
                                                                             gtgCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaac                                                                          <  3:1587123/150‑1 (MQ=255)
                                                                               gCGTGTACAACAAAACATCGAAGCGGTGAATACTGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgt                                                                       <  8:1534760/151‑1 (MQ=255)
                                                                                    tACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTg                                                                  >  2:635812/1‑151 (MQ=255)
                                                                                     aCAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTgg                                                                 >  2:1568742/1‑151 (MQ=255)
                                                                                        aGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGCCtt                                                              >  8:1591859/1‑151 (MQ=255)
                                                                                          aaaaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGATGAGAACAAACCGAACCGTGTCTGGCTTTc                                                            >  6:275383/1‑151 (MQ=255)
                                                                                          aaaaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTc                                                            >  8:1266469/1‑151 (MQ=255)
                                                                                           aaaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCTATATCCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTCGCTTTcc                                                           >  2:699113/1‑151 (MQ=255)
                                                                                           aaaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTcc                                                           <  3:1456481/151‑1 (MQ=255)
                                                                                               aTCGAAGCGGTGAATACGGTTCTTGTAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                       <  4:1388264/151‑1 (MQ=255)
                                                                                               aTCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                       <  8:469612/151‑1 (MQ=255)
                                                                                                 cGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAg                                                     <  1:1318188/151‑1 (MQ=255)
                                                                                                 cGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcaca                                                      <  1:579749/150‑1 (MQ=255)
                                                                                                  gAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                       >  8:1002149/1‑148 (MQ=255)
                                                                                                         tGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGCACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGc                                             >  2:934959/1‑151 (MQ=255)
                                                                                                         tGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGc                                             >  3:189168/1‑151 (MQ=255)
                                                                                                          gAATACGGTTCTTGAAGAGATCGACGCTCATGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGc                                             <  1:1041517/150‑1 (MQ=255)
                                                                                                          gAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGc                                             <  6:776074/150‑1 (MQ=255)
                                                                                                              aCGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCgggg                                         >  2:1107655/1‑150 (MQ=255)
                                                                                                                    cTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACcac                                  >  2:392307/1‑151 (MQ=255)
                                                                                                                      tGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACca                                   <  1:176691/148‑1 (MQ=255)
                                                                                                                       gAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGc                               <  3:55391/151‑1 (MQ=255)
                                                                                                                       gAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGc                               <  2:1298457/151‑1 (MQ=255)
                                                                                                                         agagATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCtt                             <  4:640450/151‑1 (MQ=255)
                                                                                                                          gagaTCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCttt                            <  2:798447/151‑1 (MQ=255)
                                                                                                                           agaTCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCtttt                           <  1:141182/151‑1 (MQ=255)
                                                                                                                               cGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTc                         >  1:1006293/1‑149 (MQ=255)
                                                                                                                               cGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAg                       <  1:1605723/151‑1 (MQ=255)
                                                                                                                                gACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAgg                      >  7:800096/1‑151 (MQ=255)
                                                                                                                                 aCGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGc                     >  5:1454333/1‑151 (MQ=255)
                                                                                                                                   gCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCtt                   >  5:1001234/1‑151 (MQ=255)
                                                                                                                                      cACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGCCCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTg                 >  1:976928/1‑150 (MQ=255)
                                                                                                                                       aCGAGATACCAACCCTGCTGGTGATGCACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGAc               <  6:1118116/151‑1 (MQ=255)
                                                                                                                                         gagaTCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACgg             >  4:118406/1‑151 (MQ=255)
                                                                                                                                           gaTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAg           <  6:137516/151‑1 (MQ=255)
                                                                                                                                            aTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGTAg           <  1:1228511/150‑1 (MQ=255)
                                                                                                                                                cAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCt      <  5:1192014/151‑1 (MQ=255)
                                                                                                                                                cAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACGAGAGCTTTTTCAGGCTTTGACGGAGCGGCt      <  4:190851/151‑1 (MQ=255)
                                                                                                                                                 aaCCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCtt     >  5:1236230/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCC  >  NC_000913/4401459‑4401757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑