Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,061,9160CT55.5% 138.9 / inf 509A356V (GCC→GTC) scpAmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base C (118/106);  new base T (145/134);  total (265/244)
Fisher's exact test for biased strand distribution p-value = 9.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-02

Only 100 of 514 total aligned reads displayed.

CACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATC  >  NC_000913/3061766‑3062064
                                                                                                                                                      |                                                                                                                                                    
cacaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGc                                                                                                                                                      >  7:1288460/1‑151 (MQ=255)
  caGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCt                                                                                                                                                    >  6:156135/1‑151 (MQ=255)
     gACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTg                                                                                                                                                 <  6:494734/151‑1 (MQ=255)
      aCCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGa                                                                                                                                                <  5:1930567/151‑1 (MQ=255)
        ccGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACg                                                                                                                                              <  7:360288/151‑1 (MQ=255)
         cGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACg                                                                                                                                              >  4:320934/1‑150 (MQ=255)
         cGAAATCACACGCGCTGCGAACCCACTCCCAGACCTCAGGATGGAGCCTGACTGAACAGGATCCCTATAACAACTTAATCCGCACCACCATTGAACAGCTGCCTGACACGCTGGGCGCTACTCAGACACTGCATACCAACGCCTTTGACg                                                                                                                                              >  2:795853/1‑150 (MQ=255)
            aaTCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAgc                                                                                                                                          <  2:983378/151‑1 (MQ=255)
                   ggCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGt                                                                                                                                   >  1:644839/1‑151 (MQ=255)
                   ggCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTg                                                                                                                                     <  1:125060/149‑1 (MQ=255)
                      gcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTg                                                                                                                                <  4:1003010/151‑1 (MQ=255)
                      gcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTg                                                                                                                                <  7:445685/151‑1 (MQ=255)
                          cGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTa                                                                                                                            <  6:284280/151‑1 (MQ=255)
                           gTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTAc                                                                                                                           >  1:309214/1‑151 (MQ=255)
                           gTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTAc                                                                                                                           <  7:1966421/151‑1 (MQ=255)
                             aCCGACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGGAGCGCTTGGTTTGCCTAcc                                                                                                                          <  3:122675/150‑1 (MQ=255)
                             aCCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTAcc                                                                                                                          <  7:1131181/150‑1 (MQ=255)
                               ccACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGAt                                                                                                                       >  7:1783542/1‑151 (MQ=255)
                                cACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGAtt                                                                                                                      >  3:662858/1‑151 (MQ=255)
                                cACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGAtt                                                                                                                      <  6:884074/151‑1 (MQ=255)
                                cACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCCTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGCGCGGTACTCAGTCACTCCATACCAACGCCTTTGAAGAAGCGCTTGGTTTGCCTACCGAtt                                                                                                                      >  5:514652/1‑151 (MQ=255)
                                 aCTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGAttt                                                                                                                     <  6:1697257/151‑1 (MQ=255)
                                  cTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtc                                                                                                                    >  8:1133746/1‑151 (MQ=255)
                                   tGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtct                                                                                                                   >  2:365729/1‑151 (MQ=255)
                                    gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                  <  4:596029/151‑1 (MQ=255)
                                    gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                  <  3:144040/151‑1 (MQ=255)
                                    gCCAGACCTCAGGCTGGAGCCTGACTGAACACGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                  <  5:752754/151‑1 (MQ=255)
                                      cAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTACCTACCGATTtctc                                                                                                                  >  5:1450599/1‑149 (MQ=255)
                                      cAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCAGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                  >  4:1801190/1‑149 (MQ=255)
                                          ccTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACg                                                                                                            <  4:110646/151‑1 (MQ=255)
                                             cAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGGTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCa                                                                                                          <  3:136743/150‑1 (MQ=255)
                                              cggCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCAtt                                                                                                        <  5:955404/150‑1 (MQ=255)
                                              aGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCAtt                                                                                                        <  5:1396753/151‑1 (MQ=255)
                                              aGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCAtt                                                                                                        <  4:1300206/151‑1 (MQ=255)
                                              aGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCAtt                                                                                                        <  8:188089/151‑1 (MQ=255)
                                               ggCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTg                                                                                                       <  4:49940/151‑1 (MQ=255)
                                               ggCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCCCGCATTg                                                                                                       >  1:1475467/1‑151 (MQ=255)
                                                 cTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGcc                                                                                                     >  7:1526525/1‑151 (MQ=255)
                                                   ggAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGAGCTTGGTTTGCATACCGATATCTCAGCACGCATAGACCg                                                                                                   >  6:191965/1‑151 (MQ=255)
                                                      gCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGGCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCaa                                                                                                <  6:1524624/151‑1 (MQ=255)
                                                        ctgactgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAac                                                                                               >  3:257121/1‑150 (MQ=255)
                                                        ctgactgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAac                                                                                               >  3:932677/1‑150 (MQ=255)
                                                            ctgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACAccc                                                                                           >  4:843912/1‑150 (MQ=255)
                                                              gaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGa                                                                                        <  6:391388/151‑1 (MQ=255)
                                                                 cAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGatc                                                                                      >  3:1656024/1‑150 (MQ=255)
                                                                  aGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGatcat                                                                                    >  8:1575010/1‑151 (MQ=255)
                                                                    gATCCGTATAACAACGTTATCCGCACCACCATTCAAGCGCTGGCTGCGACGCTGGGCGGTACTCACTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGATTGCCTACCGATTTCTCAGCACGCATTCCCCGCAACACCCAGagcatc                                                                                   >  8:411670/1‑150 (MQ=255)
                                                                      tCCGTATAACAACGTTATCCGCACCGCCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCACCACCCCGctcatcatc                                                                                >  2:24324/1‑151 (MQ=255)
                                                                       ccGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATcc                                                                               >  2:1337299/1‑151 (MQ=255)
                                                                       ccGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATcc                                                                               >  2:1851632/1‑151 (MQ=255)
                                                                        cGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATcc                                                                               >  7:732398/1‑150 (MQ=255)
                                                                           ataACAACGTTATCCGCACCACCATTGGAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTCCCGCTTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAgg                                                                            >  8:502752/1‑150 (MQ=255)
                                                                               caacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGgaaga                                                                        >  1:1329906/1‑150 (MQ=255)
                                                                               caacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGgaagaa                                                                       <  3:799511/151‑1 (MQ=255)
                                                                               caacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGgaaga                                                                        >  2:590009/1‑150 (MQ=255)
                                                                                aacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAAt                                                                      >  5:100136/1‑151 (MQ=255)
                                                                                 acGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGCTCATCATCCAGGAAGAATc                                                                     >  8:1294578/1‑151 (MQ=255)
                                                                                  ggTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCa                                                                    <  2:1516162/150‑1 (MQ=255)
                                                                                     tATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGa                                                                  >  7:248452/1‑150 (MQ=255)
                                                                                      aTCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAAc                                                                >  4:1680700/1‑151 (MQ=255)
                                                                                      aTCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAAc                                                                >  5:1280881/1‑151 (MQ=255)
                                                                                         cGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGGTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAActct                                                             <  1:1609672/151‑1 (MQ=255)
                                                                                           caccacCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGCACTCTGc                                                           >  2:1160143/1‑151 (MQ=255)
                                                                                             ccaccaTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCg                                                         <  6:1175917/151‑1 (MQ=255)
                                                                                                  aTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCg                                                    >  2:48574/1‑151 (MQ=255)
                                                                                                    tGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTc                                                  >  2:699323/1‑151 (MQ=255)
                                                                                                     gAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTc                                                  <  7:811499/150‑1 (MQ=255)
                                                                                                     gAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCg                                                 >  4:805845/1‑151 (MQ=255)
                                                                                                          gctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATccc                                            >  2:867279/1‑150 (MQ=255)
                                                                                                             ggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTg                                         <  3:1485717/151‑1 (MQ=255)
                                                                                                             ggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTg                                         <  6:1415948/151‑1 (MQ=255)
                                                                                                             ggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTg                                         <  8:1483122/151‑1 (MQ=255)
                                                                                                              gctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTgg                                        >  2:1680022/1‑151 (MQ=255)
                                                                                                                tgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGcc                                      >  5:1183016/1‑151 (MQ=255)
                                                                                                                tgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGcc                                      >  3:471070/1‑151 (MQ=255)
                                                                                                                     cGCTGGGCGGTACTCAGTCACTGCATACAAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAAGACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCAGCACCGTCGATCCACTGGCAGGAt                                  >  5:352607/1‑150 (MQ=255)
                                                                                                                         gggCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCt                               <  8:275075/149‑1 (MQ=255)
                                                                                                                         gggCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTa                              <  6:795042/150‑1 (MQ=255)
                                                                                                                           gCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAtt                            >  4:1196322/1‑150 (MQ=255)
                                                                                                                              gTAGTCAGTCACTGCATACCAACGCCTTTGAAGAAGCGCTTGGTTTGCCTACAGATTTCTCAGCACGCATTGCGCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACAt                        <  2:1057385/151‑1 (MQ=255)
                                                                                                                                aCTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACAt                        >  6:645109/1‑149 (MQ=255)
                                                                                                                                aCTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTg                      <  3:1809401/151‑1 (MQ=255)
                                                                                                                                 cTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTg                      >  8:852708/1‑150 (MQ=255)
                                                                                                                                   cAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGa                     <  5:683227/149‑1 (MQ=255)
                                                                                                                                    cgTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTc                  <  5:1773913/150‑1 (MQ=255)
                                                                                                                                    aGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTc                  <  7:904847/151‑1 (MQ=255)
                                                                                                                                    aGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTc                  >  3:1721871/1‑151 (MQ=255)
                                                                                                                                       cACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCt               <  1:1688016/151‑1 (MQ=255)
                                                                                                                                        aCTGCATACCATCGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTg              >  4:1633644/1‑151 (MQ=255)
                                                                                                                                           gCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGAcc           >  1:1919144/1‑151 (MQ=255)
                                                                                                                                           gCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGAcc           <  8:1705033/151‑1 (MQ=255)
                                                                                                                                           gCATACCAACGTCTTTGACGAAGCGCGTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGAcc           <  4:197457/151‑1 (MQ=255)
                                                                                                                                             aTACGAACGACTTTGACGAAGCGCTTGGTTTGCATAACGATTTCTCATCACTCATTGCCCGAAACACCCAGATGATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCTATCCACTGGCCGTATCATATTACATTTAGTCGCTTTCCGa         <  1:501597/151‑1 (MQ=255)
                                                                                                                                             aTACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCg          >  1:1019757/1‑150 (MQ=255)
                                                                                                                                              tACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGAt        >  5:1607672/1‑151 (MQ=255)
                                                                                                                                               aCCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATc       <  4:1490809/151‑1 (MQ=255)
                                                                                                                                               aCCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATc       <  6:1263790/151‑1 (MQ=255)
                                                                                                                                               aCCAACGCCTTTGACGAAGCGCTTGGTGTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGGCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATc       <  8:350404/151‑1 (MQ=255)
                                                                                                                                                  aaCGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCaa     >  1:836606/1‑150 (MQ=255)
                                                                                                                                                  aaCGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCaa     >  3:1550572/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATC  >  NC_000913/3061766‑3062064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑