Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,1570CT54.4% 73.9 / inf 401Q165Q (CAG→CAA) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (102/81);  new base T (118/100);  total (220/181)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

Only 100 of 414 total aligned reads displayed.

CGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGAC  >  NC_000913/3256007‑3256306
                                                                                                                                                      |                                                                                                                                                     
cGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCTCTGCCACACACGCctgc                                                                                                                                                       >  1:178624/1‑151 (MQ=255)
  aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                                                                                                                                                     >  2:1045136/1‑151 (MQ=255)
   tAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTgg                                                                                                                                                    >  6:1629160/1‑151 (MQ=255)
    aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTgg                                                                                                                                                    <  4:857451/150‑1 (MQ=255)
     aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGt                                                                                                                                                  <  2:456157/151‑1 (MQ=255)
      cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGt                                                                                                                                                  >  7:438995/1‑150 (MQ=255)
            aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGAGTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAagacc                                                                                                                                           >  5:221876/1‑151 (MQ=255)
            aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCACCGGAGACTCTTGCTCGCCCTCAGCCACACACGCCTGCTGGGTAAacacc                                                                                                                                           >  2:1728091/1‑151 (MQ=255)
               tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAacaccac                                                                                                                                         <  3:1918380/150‑1 (MQ=255)
                aGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacac                                                                                                                                       >  4:659261/1‑151 (MQ=255)
                 gCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacacc                                                                                                                                      <  4:176874/151‑1 (MQ=255)
                      gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGt                                                                                                                                 >  1:409236/1‑151 (MQ=255)
                      gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGt                                                                                                                                 <  6:1510826/151‑1 (MQ=255)
                      gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGt                                                                                                                                 <  3:1540662/151‑1 (MQ=255)
                      gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCGGCTGGGTAAACACCACACCATCGt                                                                                                                                 >  4:930331/1‑151 (MQ=255)
                       cgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGCTCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCGTGTTGGGTAAACACCACACCATCGTg                                                                                                                                <  5:1298281/151‑1 (MQ=255)
                        ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGc                                                                                                                               <  5:8368/151‑1 (MQ=255)
                        ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGc                                                                                                                               >  3:1055754/1‑151 (MQ=255)
                        ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc                                                                                                                               >  7:1799486/1‑151 (MQ=255)
                          aaTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAGGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGt                                                                                                                             <  6:974798/151‑1 (MQ=255)
                            tCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGtct                                                                                                                           >  7:1525606/1‑151 (MQ=255)
                            tCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTAGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct                                                                                                                           >  6:1822313/1‑151 (MQ=255)
                             cGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGtct                                                                                                                           <  6:389786/150‑1 (MQ=255)
                             cGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct                                                                                                                           <  4:140688/150‑1 (MQ=255)
                              gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGACCTTCAGGATCTCAGCCAGCGTCGTGCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCg                                                                                                                         <  8:1967833/151‑1 (MQ=255)
                              gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCg                                                                                                                         <  6:1592797/151‑1 (MQ=255)
                              gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                                                                                                         <  3:1833055/151‑1 (MQ=255)
                                 aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGata                                                                                                                      <  7:736224/151‑1 (MQ=255)
                                  aTAACGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGGCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGatat                                                                                                                     <  8:755594/151‑1 (MQ=255)
                                  aTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGatat                                                                                                                     <  4:447298/151‑1 (MQ=255)
                                   tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATg                                                                                                                    >  7:247028/1‑151 (MQ=255)
                                   tAAAGCGGATCGCCGCAAACGCGACTTCATTGACCCACTTCAGGATCTCAGCCAGCCTCGTTCTCGAAAGCACCGTCAGCGGAGACTCATCCTCGCCCTCTGCCACACACGCCTGTTGGCTAAACACCACACCATCCTGCGTCTCGATATg                                                                                                                    >  2:1435107/1‑151 (MQ=255)
                                     aaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGc                                                                                                                   >  4:1812401/1‑150 (MQ=255)
                                      aGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                                                                                                 <  8:1706179/151‑1 (MQ=255)
                                       gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                                                                                                 >  8:664694/1‑150 (MQ=255)
                                            tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGtt                                                                                                           >  5:930686/1‑151 (MQ=255)
                                             cgccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTg                                                                                                          <  6:1200762/151‑1 (MQ=255)
                                              gccgcAAACGGCACTTCATTGACGACCTTCAGGATCTCCGGCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCAGACACGCCTGTTGCGTAAACACCACACCATCCTGCGTCTCGATATGCACAATGTTgg                                                                                                         <  2:1373353/151‑1 (MQ=255)
                                               ccgcAAACGGGACTTCATTGACGACCTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGt                                                                                                        <  1:22189/151‑1 (MQ=255)
                                                cgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAACCACCACACCATCGTGCGTCTCGATAAGCACAATGTTGGt                                                                                                        >  3:1691426/1‑150 (MQ=255)
                                                cgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGt                                                                                                        >  6:733887/1‑150 (MQ=255)
                                                  cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATg                                                                                                     >  2:18330/1‑151 (MQ=255)
                                                    aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                                                                                                   >  8:1046264/1‑151 (MQ=255)
                                                      cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccg                                                                                                 >  7:606045/1‑151 (MQ=255)
                                                             tCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacg                                                                                           <  2:897378/150‑1 (MQ=255)
                                                              cATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                                                                          <  1:1360926/150‑1 (MQ=255)
                                                              cATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                                                                          <  3:84914/150‑1 (MQ=255)
                                                               aTTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATg                                                                                        >  2:162197/1‑151 (MQ=255)
                                                                 tGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGt                                                                                      >  4:1033934/1‑151 (MQ=255)
                                                                  gccGAACTTCAGGATCTCAGCCAGCGGCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGt                                                                                      <  2:655884/148‑1 (MQ=255)
                                                                   aCGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGa                                                                                    <  6:316369/151‑1 (MQ=255)
                                                                    cGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGac                                                                                   >  7:1106144/1‑151 (MQ=255)
                                                                    cGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGac                                                                                   >  8:1523955/1‑151 (MQ=255)
                                                                    cGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGac                                                                                   >  6:109446/1‑151 (MQ=255)
                                                                     gAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGaca                                                                                  <  4:1886524/151‑1 (MQ=255)
                                                                       aCTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCAGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATCCCCCCCGACGATGGTGacac                                                                                 >  8:1589887/1‑150 (MQ=255)
                                                                        cTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacacac                                                                               >  3:675266/1‑151 (MQ=255)
                                                                          tCAGGATCTCAGCCAGCGTCGTTCTGGCAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacacac                                                                               <  7:1407673/149‑1 (MQ=255)
                                                                          tCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGc                                                                             >  2:217979/1‑151 (MQ=255)
                                                                               aTCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACt                                                                        >  1:575482/1‑151 (MQ=255)
                                                                                  tcAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTtc                                                                      >  5:391060/1‑150 (MQ=255)
                                                                                   cagccagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTtctc                                                                    <  8:560694/151‑1 (MQ=255)
                                                                                   cagccagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCAc                                                                         <  2:1094683/146‑1 (MQ=255)
                                                                                      ccagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCaac                                                                 <  3:543479/151‑3 (MQ=255)
                                                                                       cagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCg                                                                <  2:1298236/151‑1 (MQ=255)
                                                                                           gtcgtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTcc                                                            >  6:1713494/1‑151 (MQ=255)
                                                                                            tcgtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTcc                                                            >  8:870964/1‑150 (MQ=255)
                                                                                              gtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTcc                                                            >  5:1241245/1‑148 (MQ=255)
                                                                                                tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACt                                                       >  8:1301729/1‑151 (MQ=255)
                                                                                                tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACt                                                       >  6:66968/1‑151 (MQ=255)
                                                                                                   ggAAAGCACCGGCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTg                                                    <  5:1859031/151‑1 (MQ=255)
                                                                                                    gAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGGCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTgg                                                   <  1:1000451/151‑1 (MQ=255)
                                                                                                     aaaGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGgc                                                  <  4:1133779/151‑1 (MQ=255)
                                                                                                      aaGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACTCACGCCCACTTCTCACCGTTCCAGACTTTGgcg                                                 <  2:1882828/151‑1 (MQ=255)
                                                                                                      aaGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGgcg                                                 <  3:1327051/151‑1 (MQ=255)
                                                                                                          aCCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTg                                             >  1:854871/1‑151 (MQ=255)
                                                                                                            cGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTgag                                           <  2:545606/151‑1 (MQ=255)
                                                                                                               cAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTgaga                                          >  3:1231994/1‑149 (MQ=255)
                                                                                                               cAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGagaag                                        <  6:575652/151‑1 (MQ=255)
                                                                                                                aGCGGAGACTCTTGCTCGCCCTCTGCCACACACGGCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTTGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGagaaga                                       <  8:437715/151‑1 (MQ=255)
                                                                                                                              cTCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGct                          >  4:317026/1‑149 (MQ=255)
                                                                                                                               tCGCCCTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCa                          >  6:1360160/1‑149 (MQ=255)
                                                                                                                               tCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCa                          >  3:176671/1‑149 (MQ=255)
                                                                                                                                  cccTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGtt                     <  5:1610578/151‑1 (MQ=255)
                                                                                                                                  cccTCTGACACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGAACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCAACTTATCACCGTTCCAGCCTTTGGAGCGTGAGAATAGGAGTTCATCGCAAGGtt                     >  1:1191661/1‑151 (MQ=255)
                                                                                                                                   ccTCTGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTc                    <  3:412938/151‑1 (MQ=255)
                                                                                                                                     tctGCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCt                  >  6:881913/1‑151 (MQ=255)
                                                                                                                                     tctGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCt                  >  3:1462802/1‑151 (MQ=255)
                                                                                                                                      ctGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTg                 >  5:1750752/1‑151 (MQ=255)
                                                                                                                                        gCCACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGa               <  4:226735/151‑1 (MQ=255)
                                                                                                                                        gCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTgg                <  8:326244/150‑1 (MQ=255)
                                                                                                                                         ccACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGAt              <  4:1542241/151‑1 (MQ=255)
                                                                                                                                         ccACACACGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGAt              <  4:1335282/151‑1 (MQ=255)
                                                                                                                                            cacacGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCtt           <  7:1929151/151‑1 (MQ=255)
                                                                                                                                             acacGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGCTCtt           >  5:542518/1‑150 (MQ=255)
                                                                                                                                              cacGCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTaa         <  2:1159721/151‑1 (MQ=255)
                                                                                                                                                 gCCTGTTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACgg      <  1:1516584/151‑1 (MQ=255)
                                                                                                                                                 gCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGAATGCCCGCCGACGATGGTGACACACGCCAACTTCTCACCGTACCAGACTTTGCCGCCTGAGAATAGGATTTCATCGCAACGTTCCTGGATCTTAACgg      >  5:1454639/1‑151 (MQ=255)
                                                                                                                                                   ctgctgGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGCTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCCCAAGGTTCCTGGATCTTAACGgac    >  2:719555/1‑150 (MQ=255)
                                                                                                                                                     gctgGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGgag    <  6:1046143/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGAC  >  NC_000913/3256007‑3256306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑