Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913290,1750GA54.4% 108.4 / inf 537T44I (ACC→ATC) argFornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base G (136/108);  new base A (152/139);  total (288/249)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

Only 100 of 539 total aligned reads displayed.

GCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAAC  >  NC_000913/290025‑290325
                                                                                                                                                      |                                                                                                                                                       
   gtgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTa                                                                                                                                                       >  7:210668/1‑150 (MQ=255)
     gtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAg                                                                                                                                                     <  3:81834/150‑1 (MQ=255)
     gtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGc                                                                                                                                                    >  1:1697672/1‑151 (MQ=255)
          tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTATg                                                                                                                                               >  7:1750376/1‑151 (MQ=255)
           aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGt                                                                                                                                              >  4:1747653/1‑151 (MQ=255)
           aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGt                                                                                                                                              >  1:1073990/1‑151 (MQ=255)
             ttGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTAc                                                                                                                                            >  1:1930658/1‑151 (MQ=255)
               gACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACtt                                                                                                                                          >  3:1070655/1‑151 (MQ=255)
                   ctTTCTGCCCAATCTGGCTGCCGCTCGGCCCTATATAGGTATCGCGCGCGCCCTGGTCTAATGCGGCAACTTCGAAAGAGCAACGCGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTcc                                                                                                                                        <  5:829452/149‑1 (MQ=18)
                        tGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCCAAAGAGCAACGGGTACGAGACGAGTCTTTTTCGAAGATGTGCGCGAAGAATTTACCGGTAAGCTTCTGTACATCCTTGGCA‑t                                                                                                                                >  4:1329210/1‑151 (MQ=255)
                         gCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGGCA‑tt                                                                                                                               >  1:314546/1‑151 (MQ=255)
                             aaTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑tttttt                                                                                                                           <  4:867141/151‑1 (MQ=255)
                             aaTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑tttttt                                                                                                                           >  6:1154118/1‑151 (MQ=255)
                                 tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTAt                                                                                                                       >  4:1472168/1‑151 (MQ=255)
                                    cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCgg                                                                                                                    >  7:458463/1‑151 (MQ=255)
                                      gCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCgg                                                                                                                    <  7:1131136/149‑1 (MQ=255)
                                        cGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGGAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCttt                                                                                                                <  5:1527561/151‑1 (MQ=255)
                                          cTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTCCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTgc                                                                                                              >  1:739052/1‑150 (MQ=255)
                                           tCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGCGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGGTTTg                                                                                                               >  8:298723/1‑149 (MQ=255)
                                           tCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTg                                                                                                               >  7:1602287/1‑149 (MQ=255)
                                            cGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGa                                                                                                              <  7:1466038/149‑1 (MQ=255)
                                             ggCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCt                                                                                                           >  4:336065/1‑151 (MQ=255)
                                             ggCCCTAAATAGGTAACGCGCGCGCCCTGCTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCt                                                                                                           <  8:1271183/151‑1 (MQ=255)
                                             cgCCCTAAATAGGTAACCCGCGCGCCCAGGTCAACTGCGCCAACTTCGAAAGAGCAACGGGGACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGGTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCt                                                                                                           <  2:1278042/150‑1 (MQ=25)
                                               cccTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCtgt                                                                                                         <  7:78689/151‑1 (MQ=255)
                                                   aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCgg                                                                                                     >  2:1770789/1‑151 (MQ=255)
                                                   aaaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCgg                                                                                                     >  1:1424540/1‑151 (MQ=255)
                                                    aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGc                                                                                                    >  2:658155/1‑151 (MQ=255)
                                                    aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGc                                                                                                    >  2:107473/1‑151 (MQ=255)
                                                    aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGGCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGc                                                                                                    <  5:1902633/151‑1 (MQ=255)
                                                     aTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGc                                                                                                    >  7:316939/1‑150 (MQ=255)
                                                                gcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagc                                                                                          >  8:1580078/1‑149 (MQ=255)
                                                                  gcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCagag                                                                                      <  6:733616/151‑1 (MQ=255)
                                                                    gcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGaa                                                                                    <  3:296863/151‑1 (MQ=255)
                                                                      ccTGGTCAACTGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGt                                                                                  <  1:1626317/151‑1 (MQ=255)
                                                                      ccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGt                                                                                  <  2:1603469/151‑1 (MQ=255)
                                                                       cTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGTCAAGGGTCAGCAGAGAACTg                                                                                 >  5:533349/1‑151 (MQ=255)
                                                                        tGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTg                                                                                 >  4:1579311/1‑150 (MQ=255)
                                                                         ggTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTg                                                                                 <  3:232696/149‑1 (MQ=255)
                                                                         ggTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGa                                                                                >  2:1171112/1‑150 (MQ=255)
                                                                            cAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACt                                                                             <  5:145935/150‑1 (MQ=255)
                                                                            cAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACtg                                                                            >  1:616304/1‑151 (MQ=255)
                                                                                   ggCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAggg                                                                     <  6:956473/151‑1 (MQ=255)
                                                                                     cAAATTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGt                                                                   >  1:861943/1‑151 (MQ=255)
                                                                                        cTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTaa                                                                 >  5:427936/1‑150 (MQ=255)
                                                                                          tCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGATGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGt                                                              >  5:692945/1‑151 (MQ=255)
                                                                                          tCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTaaa                                                                <  3:521405/149‑1 (MQ=255)
                                                                                          tCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAg                                                               <  5:102326/150‑1 (MQ=255)
                                                                                           cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGt                                                              <  4:1957378/150‑1 (MQ=255)
                                                                                               agatCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGTTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGc                                                         <  3:451103/151‑1 (MQ=255)
                                                                                               agagCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGCGCTGTGCGGCAAGGGTCAGCACAGAAGTGAACTGTGGAGGGGGAAAGTCGatc                                                         >  3:1763761/1‑149 (MQ=37)
                                                                                                 agCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGc                                                         >  8:485229/1‑149 (MQ=255)
                                                                                                   cAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGtt                                                     <  6:147840/151‑1 (MQ=255)
                                                                                                    aaCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttt                                                    <  6:1516845/151‑1 (MQ=255)
                                                                                                    aaCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttt                                                    >  2:1935101/1‑151 (MQ=255)
                                                                                                     aCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttt                                                    >  7:1912077/1‑150 (MQ=255)
                                                                                                     aCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTc                                                   <  3:151418/151‑1 (MQ=255)
                                                                                                      cGGGTACGAGTCGAGTGGTTTTCGAAGATGCGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCACTTTcg                                                  >  2:339767/1‑150 (MQ=255)
                                                                                                      cGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTGTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCGGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCa                                                  <  4:1200262/151‑1 (MQ=255)
                                                                                                         gTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGaa                                               <  4:568212/151‑1 (MQ=255)
                                                                                                          tACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCTA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGaa                                               >  8:1483663/1‑150 (MQ=255)
                                                                                                          tACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTTAAGTGGAGCAGTTTGAGaa                                               >  2:801217/1‑150 (MQ=255)
                                                                                                           aCGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGaaa                                              <  4:1637838/150‑1 (MQ=255)
                                                                                                           aCGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAg                                             <  8:1803440/151‑1 (MQ=255)
                                                                                                           aCGAGTCGACGCTTTTTCGCACATGAGCGCGAAGTTTTTACCGGTAACCTTCTGTACTTCCATGCCA‑TTTTTTTTATCGGCGGTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAg                                             <  8:1007463/151‑1 (MQ=255)
                                                                                                             gagtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGGAGTTTCAGAAAgtg                                           >  2:907163/1‑151 (MQ=255)
                                                                                                              agtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAgtgt                                          <  8:603422/151‑1 (MQ=255)
                                                                                                              agtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAgtg                                           <  8:863690/150‑1 (MQ=255)
                                                                                                              agtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGGTTCAGAAAgtgt                                          >  6:1274366/1‑151 (MQ=255)
                                                                                                                 cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGtttt                                       <  7:636147/151‑1 (MQ=255)
                                                                                                                 cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGttt                                        <  6:827246/150‑1 (MQ=255)
                                                                                                                 cgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTGCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGttt                                        <  1:268189/150‑1 (MQ=255)
                                                                                                                  gagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGttttt                                      <  6:360593/151‑1 (MQ=255)
                                                                                                                   agtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGtttttt                                     <  8:1097504/151‑1 (MQ=255)
                                                                                                                   agtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGGCGAGCAGTTTCAGAAAGTGtttttt                                     <  5:1803311/151‑1 (MQ=255)
                                                                                                                     tcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTAGCCA‑‑TTTTTTTATCGGCATTGAGCAGTGCGGCAAAGCTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTACAGAAAGTGTTTTTTGt                                   >  1:487690/1‑150 (MQ=255)
                                                                                                                      cTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGt                                   >  3:1211575/1‑150 (MQ=255)
                                                                                                                       tttttCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑‑TTTTTTTATCGGCTTTGAGCTGTGCGGCAAAGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGtat                                 >  8:1747220/1‑150 (MQ=255)
                                                                                                                        ttttCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGt                                   <  6:852612/148‑1 (MQ=255)
                                                                                                                         tttCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGtata                                <  4:1811685/150‑1 (MQ=255)
                                                                                                                           tCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATaaa                              <  1:1286545/150‑1 (MQ=255)
                                                                                                                           tCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATaaa                              <  2:466628/150‑1 (MQ=255)
                                                                                                                           tCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAAt                             >  1:805122/1‑151 (MQ=255)
                                                                                                                            cGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTAAGAAAGTGTATTTTGTATAAATc                            >  7:547439/1‑151 (MQ=255)
                                                                                                                            cGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAAt                             >  4:68062/1‑151 (MQ=255)
                                                                                                                             gAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCg                           <  3:1372315/151‑1 (MQ=255)
                                                                                                                              aaGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCgg                          >  6:1682482/1‑151 (MQ=255)
                                                                                                                                 aTGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGAc                        >  6:1902401/1‑150 (MQ=255)
                                                                                                                                  tGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACAt                      >  3:1836913/1‑151 (MQ=255)
                                                                                                                                   gAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACAtt                     >  4:452517/1‑151 (MQ=255)
                                                                                                                                    aGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑‑TTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACAtttt                   <  6:521958/151‑1 (MQ=255)
                                                                                                                                         gATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATcc               >  3:922055/1‑151 (MQ=255)
                                                                                                                                          aTGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTAAGGGGTTTGGGATTTCCGGAAGGGGGCGGATGAAAGGTGACGTGTGAAGGGGTAACTGCGGGAATTTTAAGAAAGGGTTTTTTGTTTAATGGGGAAATTTTaact               >  8:427836/1‑147 (MQ=255)
                                                                                                                                            gTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCg            >  7:859674/1‑151 (MQ=255)
                                                                                                                                            gTTTTTACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTc             <  2:1491054/150‑1 (MQ=255)
                                                                                                                                                ttACCGATAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATg        >  5:343495/1‑151 (MQ=255)
                                                                                                                                                  aCCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATgg       <  5:1257061/150‑1 (MQ=255)
                                                                                                                                                  aCCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGAAATTTTATCCTCGAATGGc      >  5:247342/1‑151 (MQ=255)
                                                                                                                                                   ccGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCg     >  3:726696/1‑151 (MQ=255)
                                                                                                                                                    cGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGa    <  3:620680/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GCGGTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAAC  >  NC_000913/290025‑290325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑