Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,297,4960GA58.4% 251.7 / inf 479A209V (GCT→GTT) adhEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
Reads supporting (aligned to +/- strand):  ref base G (116/82);  new base A (142/136);  total (260/219)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 484 total aligned reads displayed.

AAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAG  >  NC_000913/1297347‑1297646
                                                                                                                                                      |                                                                                                                                                      
aaaCAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGc                                                                                                                                                       >  1:518811/1‑151 (MQ=255)
  aCAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTg                                                                                                                                                     <  4:771973/151‑1 (MQ=255)
    agagTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGt                                                                                                                                                   >  7:1070917/1‑151 (MQ=255)
         caacaacaacaaCAGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTAcc                                                                                                                                              >  3:1498906/1‑151 (MQ=255)
          aacaacaacaacAGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACCCCGATAGCTGGTTTACCg                                                                                                                                             >  7:1330783/1‑151 (MQ=255)
           acaacaacaacaGACTGTTCAGAAGCACAGATTACGGCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCgg                                                                                                                                            <  6:1940245/151‑1 (MQ=255)
              acaacaacaGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGc                                                                                                                                         <  1:1901795/151‑1 (MQ=255)
                  caacaGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTa                                                                                                                                     <  4:847976/151‑1 (MQ=255)
                   aacaGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCTCGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTAt                                                                                                                                    <  8:1402647/151‑1 (MQ=255)
                   aacaGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTAt                                                                                                                                    <  6:1368282/151‑1 (MQ=255)
                   aacaGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTAt                                                                                                                                    >  3:1720867/1‑151 (MQ=255)
                   aacaGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTAt                                                                                                                                    >  8:514381/1‑151 (MQ=255)
                       gACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGATTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGATTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACAACGATAACAGGTTTACCGGAGCTGTATGCgc                                                                                                                                >  2:1886829/1‑150 (MQ=255)
                        aCTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGc                                                                                                                               <  7:1580929/151‑1 (MQ=255)
                         cTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCt                                                                                                                              >  7:1011976/1‑151 (MQ=255)
                         cTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCACTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTCGAGTCTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGACCTGTATGCCGc                                                                                                                               >  1:1529155/1‑150 (MQ=255)
                            ttCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCttt                                                                                                                            >  6:1232853/1‑150 (MQ=255)
                             tCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTa                                                                                                                           >  4:707769/1‑150 (MQ=255)
                              cAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAAc                                                                                                                         >  2:954652/1‑151 (MQ=255)
                               aGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATTCGGCTTTAAcc                                                                                                                        <  7:1424295/151‑1 (MQ=255)
                                 aaGCACAGATTATGCCGTTTGTC‑AACGTTTTGGACATTATTTCAGCAGGAACTTCACGTTTGATATCATCATTTTCATCGATAACAACTTGATTGTTGGCCGCGCCTACACCGATACCTGGTTTACCGGAGCTGTATGCGGCTTTAACCAt                                                                                                                      <  2:1161837/151‑1 (MQ=255)
                                 aaGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCAt                                                                                                                      <  4:1010029/151‑1 (MQ=255)
                                   gCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGc                                                                                                                    <  2:1664464/151‑1 (MQ=255)
                                      cacaTTAGGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCATCTGCACGTGTGATATCAGGAGTTTCATCGATAACTACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAAGCATGCCCg                                                                                                                 <  1:1208570/148‑1 (MQ=255)
                                      caGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCg                                                                                                                 <  3:1940449/151‑1 (MQ=255)
                                      caGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCCATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCg                                                                                                                 >  7:475668/1‑151 (MQ=255)
                                        gATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCgg                                                                                                                >  5:1383255/1‑150 (MQ=255)
                                            accccG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGacca                                                                                                            <  5:1891974/147‑1 (MQ=255)
                                             cGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGaccacc                                                                                                          >  8:1732978/1‑151 (MQ=255)
                                               ccG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGaccacc                                                                                                          >  5:529433/1‑149 (MQ=255)
                                                   ttGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcg                                                                                                     >  5:1722377/1‑151 (MQ=255)
                                                    tGTCGAAGGTGTTGGACATCAGTCCAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcg                                                                                                     <  5:308962/150‑1 (MQ=255)
                                                     gTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgcg                                                                                                   <  2:1233448/151‑1 (MQ=255)
                                                     gTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgcg                                                                                                   <  1:195456/151‑1 (MQ=255)
                                                     gTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgc                                                                                                    <  4:1517418/150‑1 (MQ=255)
                                                       cGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgcg                                                                                                   >  5:1963660/1‑149 (MQ=255)
                                                          aGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGAt                                                                                              <  7:663941/151‑1 (MQ=255)
                                                          aGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGAt                                                                                              <  5:457466/151‑1 (MQ=255)
                                                               ttGGACATCAGTACAGATGCAACTGCACGGTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGt                                                                                         >  1:391042/1‑151 (MQ=255)
                                                                 ggACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGtt                                                                                        <  3:880342/150‑1 (MQ=255)
                                                                   aCATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGAt                                                                                     >  8:229381/1‑151 (MQ=255)
                                                                    cATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCAAGCCCGGAGCACCAGTCGCGAGGATCACGTTGATg                                                                                    >  5:851167/1‑151 (MQ=255)
                                                                      tCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGCCCACCAGTCGCGAGGATCAGGTTGATGtg                                                                                  >  6:321101/1‑150 (MQ=255)
                                                                       cAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTc                                                                                  <  1:1741212/150‑1 (MQ=255)
                                                                         gTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTgg                                                                               <  6:864461/151‑1 (MQ=255)
                                                                           aCAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGggt                                                                             >  2:1039787/1‑151 (MQ=255)
                                                                           aCAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGggt                                                                             <  3:1384817/151‑1 (MQ=255)
                                                                            cAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGggtg                                                                            <  7:95/151‑1 (MQ=255)
                                                                             aGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGggtgg                                                                           >  1:996832/1‑151 (MQ=255)
                                                                               aTGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCCCCTAAACAGATAGCTGGTTTACCGGAGCTGAATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTggggtctg                                                                         >  7:1508370/1‑146 (MQ=255)
                                                                                  cAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCAt                                                                      >  3:601863/1‑151 (MQ=255)
                                                                                   aaCTGCACGTTTGAAATCAGCAGTTTCATCGATAACACCTGGAGCGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATc                                                                     <  5:1644521/151‑1 (MQ=255)
                                                                                    aCTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                    <  6:118603/151‑1 (MQ=255)
                                                                                       gCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTCGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCCGACCACAAGTCGCGAGGATCAGGTTGAAGTCAGGGTGGTGCAACact                                                                  >  1:971241/1‑148 (MQ=255)
                                                                                       gCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTg                                                                 <  7:1947751/151‑1 (MQ=255)
                                                                                        cACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGc                                                                >  8:1051575/1‑151 (MQ=255)
                                                                                                atatCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGAc                                                         >  5:1016101/1‑150 (MQ=255)
                                                                                                atatCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACa                                                        <  8:1529072/151‑1 (MQ=255)
                                                                                                atatCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACa                                                        <  4:378414/151‑1 (MQ=255)
                                                                                                 tatCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGAc                                                         >  5:916737/1‑149 (MQ=255)
                                                                                                 tatCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGAc                                                         >  5:991579/1‑149 (MQ=255)
                                                                                                  atCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGAATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAg                                                       <  8:1270436/150‑1 (MQ=255)
                                                                                                   tCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGtt                                                     >  8:1897951/1‑151 (MQ=255)
                                                                                                      gcagTTTCATAGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCaa                                                  <  6:1886428/151‑1 (MQ=255)
                                                                                                        agTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAAc                                                 >  6:720808/1‑150 (MQ=255)
                                                                                                        agTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAAc                                                 >  4:296950/1‑150 (MQ=255)
                                                                                                        agTTTCATCGATAACAACTGGAGGGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACa                                                <  3:438698/151‑1 (MQ=255)
                                                                                                           ttCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGa                                              >  6:1476755/1‑150 (MQ=255)
                                                                                                            tCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGa                                              >  2:1514222/1‑149 (MQ=255)
                                                                                                              aTCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGt                                          <  4:1727789/151‑1 (MQ=255)
                                                                                                                 gATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTg                                        >  1:1392785/1‑150 (MQ=255)
                                                                                                                  aTAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgat                                      <  4:1615837/151‑1 (MQ=255)
                                                                                                                  aTAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgat                                      <  5:571940/151‑1 (MQ=255)
                                                                                                                  aTAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGGATCAGTGCGTTTGACAGTTGAACAGAAGGTTgat                                      >  8:1629867/1‑151 (MQ=255)
                                                                                                                    aacaacTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcg                                    <  4:1242451/151‑1 (MQ=255)
                                                                                                                    aacaacTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcg                                    <  3:1215143/151‑1 (MQ=255)
                                                                                                                      caacTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcgat                                  >  2:1116032/1‑151 (MQ=255)
                                                                                                                        acTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATcc                                >  7:117404/1‑151 (MQ=255)
                                                                                                                         cTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGCGGATCAGGTTGATGTCTGGGTGGTTCATCAGTGCGTTAGACAGTTGAACAGAAGGTTGATAGCTccc                               >  6:1676409/1‑150 (MQ=255)
                                                                                                                          tGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGCTCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAAGAGAAGGTTGATCGATACac                              >  4:557847/1‑150 (MQ=255)
                                                                                                                          tGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGAGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCac                              >  1:598310/1‑150 (MQ=255)
                                                                                                                           ggAGTGTTGGCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACGACCAGTCGCGAGGATCAGGTTGATGTCTGTGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAg                              <  2:690728/150‑1 (MQ=255)
                                                                                                                               tgtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGAt                         >  4:1947180/1‑151 (MQ=255)
                                                                                                                               ggtTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGAt                         <  6:479607/150‑1 (MQ=255)
                                                                                                                                gtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGAt                         <  7:218109/150‑1 (MQ=255)
                                                                                                                                gtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATc                        >  5:1470909/1‑151 (MQ=255)
                                                                                                                                 ttGCCCGCGCCTACCCCGATAACTGGTTTACCGGAGCTGTATGCGCCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGCTCGATCCAGCCGATc                        >  5:216256/1‑150 (MQ=255)
                                                                                                                                 ttGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATc                        >  4:72605/1‑150 (MQ=255)
                                                                                                                                  tGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATc                        >  7:834186/1‑149 (MQ=255)
                                                                                                                                   gCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATc                        >  5:1525771/1‑148 (MQ=255)
                                                                                                                                    cccGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGAt                    <  1:1684384/151‑1 (MQ=255)
                                                                                                                                     ccGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGAt                    >  5:71438/1‑150 (MQ=255)
                                                                                                                                     ccGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATc                   <  8:938503/151‑1 (MQ=255)
                                                                                                                                         gcCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTc               <  2:458791/151‑1 (MQ=255)
                                                                                                                                          ccTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCg              >  1:1072355/1‑151 (MQ=255)
                                                                                                                                          ccTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCACGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCg              >  8:662984/1‑151 (MQ=255)
                                                                                                                                             acacCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGa            <  5:1226610/150‑1 (MQ=255)
                                                                                                                                               acCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGc          >  1:825342/1‑150 (MQ=255)
                                                                                                                                                 cGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACACAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCAcc       >  7:1663929/1‑151 (MQ=255)
                                                                                                                                                   aTAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCgg     >  4:142535/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCG‑TTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAG  >  NC_000913/1297347‑1297646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑