BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,234,479	0	C	T	47.3%	137.2 / inf	496	A241V (GCG→GTG)	pgi	glucosephosphate isomerase
Reads supporting (aligned to +/- strand): ref base C (147/113); new base T (111/122); total (261/235)
Fisher's exact test for biased strand distribution p-value = 5.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-03

Only 100 of 513 total aligned reads displayed.

AAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCG  >  NC_000913/4234329‑4234628

    gCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgcggcg                                                                                                                                                   >  5:458991/1‑151 (MQ=255)

    gCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgcggc                                                                                                                                                    <  4:1681464/150‑1 (MQ=255)

     cTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGgcgc                                                                                                                                                  >  1:1376493/1‑151 (MQ=255)

      tGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCt                                                                                                                                                 >  6:753443/1‑151 (MQ=255)

       gAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCtt                                                                                                                                                >  5:590914/1‑151 (MQ=255)

        aataaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCttt                                                                                                                                               <  3:1559606/148‑1 (MQ=255)

        aaaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCttt                                                                                                                                               >  5:1059414/1‑151 (MQ=255)

         aaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTc                                                                                                                                              >  5:733899/1‑151 (MQ=255)

          aaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTcc                                                                                                                                             <  3:1038958/151‑1 (MQ=255)

          aaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTcc                                                                                                                                             >  2:925046/1‑151 (MQ=255)

          aaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTcc                                                                                                                                             <  8:794813/151‑1 (MQ=255)

           aaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTcc                                                                                                                                             >  5:1306638/1‑150 (MQ=255)

            aaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGAAAAACACTTTGCGGCGCTTTccac                                                                                                                                           >  2:1210349/1‑151 (MQ=255)

              gTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTccacca                                                                                                                                         <  3:83869/151‑1 (MQ=255)

                aaaCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAAt                                                                                                                                       <  3:18577/151‑1 (MQ=255)

                 aaCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCCACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAGAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATg                                                                                                                                      <  8:387312/151‑1 (MQ=255)

                  aCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGc                                                                                                                                     >  8:1334977/1‑151 (MQ=255)

                   cccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGcc                                                                                                                                    >  8:212818/1‑151 (MQ=255)

                   cccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGcc                                                                                                                                    >  1:1008094/1‑151 (MQ=255)

                     cGGAACCCAGCCTTTTCTTGGTAGCATATAAAACCTACACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCaa                                                                                                                                  <  2:791127/151‑1 (MQ=255)

                     cGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCa                                                                                                                                   >  2:1029816/1‑150 (MQ=255)

                      ggAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaaa                                                                                                                                 <  4:812961/151‑1 (MQ=255)

                      ggAAACCACGCTCTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCCTGACTGGTTCCTGAAAGCGGCAGCACATGAAAAACACGATGCAAAACACTTTGCGGCGATTTCCACCAATGCCaa                                                                                                                                  >  6:1365233/1‑150 (MQ=255)

                       gAAACCACGCTGTTCTTGGTAGCATCTAACACCTTCACCACTCCGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAg                                                                                                                                <  6:1011988/151‑1 (MQ=255)

                        aaaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGc                                                                                                                               >  6:1127222/1‑151 (MQ=255)

                           ccACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGt                                                                                                                            <  8:1215299/151‑1 (MQ=255)

                            cACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGtt                                                                                                                           >  2:179290/1‑151 (MQ=255)

                            cACGCTGTTCTTGGGAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                                                                                                           <  7:1411983/151‑1 (MQ=255)

                             aCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCGGTTg                                                                                                                          <  1:947686/151‑1 (MQ=255)

                             aCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTg                                                                                                                          <  7:318411/151‑1 (MQ=255)

                              cGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                                                                                                         <  3:99911/151‑1 (MQ=255)

                              cGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                                                                                                         <  1:1201014/151‑1 (MQ=255)

                               gCTGTTCTTGCTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCTGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                                        <  8:564249/151‑1 (MQ=255)

                                       tGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                                                                                                 <  1:1069424/150‑1 (MQ=255)

                                        ggTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                                               <  4:1068210/151‑1 (MQ=255)

                                         gTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                                               >  2:522389/1‑150 (MQ=255)

                                         gTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                                                                                              <  5:1601588/151‑1 (MQ=255)

                                         gTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                                                                                              <  3:589587/151‑1 (MQ=255)

                                          tAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                                             >  7:188571/1‑151 (MQ=255)

                                           aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAtt                                                                                                            >  8:1947175/1‑151 (MQ=255)

                                               tCTAAAACCTTCACCACTCAGGAAACTATGACAAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACCTTGCAAAACACTTTGCGGCGCGTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAt                                                                                                         >  5:1358591/1‑150 (MQ=255)

                                                  aaaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                                                                                                       <  7:1626664/149‑1 (MQ=255)

                                                     aCCTTCACCACTCA‑GAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCa                                                                                                  >  8:1070253/1‑150 (MQ=255)

                                                         tCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGAGTGGTTCCTGAAGGCGGAGGGTGATGAAAACCACGTTGCAAACCACTTTCCGGCGGTTTCCACCAATGCCAACGCCGTTGGCGAGTTGGGTATTAATACTGCCAACAt                                                                                              >  8:1147489/1‑151 (MQ=255)

                                                          caccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACAt                                                                                              <  1:84126/150‑1 (MQ=255)

                                                          caccacTCACGAAACTCTGACCAACGCCCATAGCGCGCGTGCCTGGTTCCGGGAAGCGGCAGGTGATGAAAAACACCTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAACCCGTTGGCGAGTTTGGTATTGATACTGCCAACAt                                                                                              <  6:1692125/150‑1 (MQ=255)

                                                              ccTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg                                                                                         <  7:407417/150‑1 (MQ=255)

                                                              acTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg                                                                                         >  5:1845912/1‑151 (MQ=255)

                                                                 cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa                                                                                        <  8:1274974/149‑1 (MQ=255)

                                                                  aGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGtt                                                                                     <  3:1964794/151‑1 (MQ=255)

                                                                      aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgg                                                                                 <  5:530265/151‑1 (MQ=255)

                                                                        cTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGAAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgggc                                                                               >  3:1353719/1‑150 (MQ=255)

                                                                         tATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGAc                                                                              >  4:589614/1‑151 (MQ=255)

                                                                          aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACt                                                                             >  6:1818054/1‑151 (MQ=255)

                                                                          aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACt                                                                             <  7:612906/151‑1 (MQ=255)

                                                                          aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACt                                                                             >  8:1555816/1‑151 (MQ=255)

                                                                           tGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTg                                                                            >  5:1715264/1‑151 (MQ=255)

                                                                            gACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTgg                                                                           >  6:1472278/1‑151 (MQ=255)

                                                                             aCCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTggg                                                                          <  6:1884139/151‑1 (MQ=255)

                                                                               cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGtt                                                                        <  1:1936090/151‑1 (MQ=255)

                                                                                aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTg                                                                       >  3:67532/1‑151 (MQ=255)

                                                                                aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTg                                                                       <  3:441304/151‑1 (MQ=255)

                                                                                   gCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggcg                                                                    >  1:631766/1‑151 (MQ=255)

                                                                                        tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGt                                                               >  4:1863849/1‑151 (MQ=255)

                                                                                         aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGtt                                                              >  5:255379/1‑151 (MQ=255)

                                                                                             cgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTActg                                                          >  8:1840955/1‑150 (MQ=255)

                                                                                                  gACTGGGTCCTGAAAGCGGCAGGTGATGAAAACCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTGCTGGGACTGGGTTGGCGGCCGTTACTCTTtgt                                                     <  5:1966953/151‑1 (MQ=255)

                                                                                                   aCTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtgtg                                                    <  8:916085/151‑1 (MQ=255)

                                                                                                       gTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                                 >  4:687176/1‑150 (MQ=255)

                                                                                                       gTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCa                                                <  4:345136/151‑1 (MQ=255)

                                                                                                          ccTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCg                                             >  4:112064/1‑151 (MQ=255)

                                                                                                          ccTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCg                                             >  2:647333/1‑151 (MQ=255)

                                                                                                             gAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                                           <  5:1188679/150‑1 (MQ=255)

                                                                                                              aaaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTg                                         >  3:31306/1‑151 (MQ=255)

                                                                                                              aaaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGGTGGCGGCCGTTCCTCTTTGTGGTCAGCGATTg                                         >  1:1776608/1‑151 (MQ=255)

                                                                                                                aGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGc                                       >  2:1379447/1‑151 (MQ=255)

                                                                                                                   ggCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                    <  3:179280/151‑1 (MQ=255)

                                                                                                                      aGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCg                                 >  7:985296/1‑151 (MQ=255)

                                                                                                                      aGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCg                                 <  7:1054524/151‑1 (MQ=255)

                                                                                                                        gTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt                               <  5:833535/151‑1 (MQ=255)

                                                                                                                          gatgaAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGTCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTg                             <  3:1237188/151‑1 (MQ=255)

                                                                                                                          gatgaAAAACACGGTCCAAAACACTTTGCGTCGCTTTCCACCAATGCCAAAGCCTTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGTACTGGGTTGGCGGCCGTTACTCTTTGTGGGCAGCGATTGGCATGTCGAtt                              <  6:1532444/150‑1 (MQ=255)

                                                                                                                              aaaaaCACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtct                         >  2:1532748/1‑151 (MQ=255)

                                                                                                                               aaaaCACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctc                        >  6:1239860/1‑151 (MQ=255)

                                                                                                                                   cacGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCa                    <  7:211705/151‑1 (MQ=255)

                                                                                                                                    acGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGTTTGTTGTCTGCAt                   >  4:442765/1‑151 (MQ=255)

                                                                                                                                     cGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                  >  1:386931/1‑151 (MQ=255)

                                                                                                                                      gTTTCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCg                 <  7:522172/151‑1 (MQ=255)

                                                                                                                                        tGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGCTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCggg               >  2:478194/1‑150 (MQ=255)

                                                                                                                                        tGCAAAAAACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGACAACATGTTAGAGTTCTGGGACTGCGATGGCGGCCCTAACTCAATGTGGTGAGAAATTGTACTTTAGATTGTTCTATACAtaga                >  6:624220/1‑147 (MQ=255)

                                                                                                                                         gCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGc               <  7:875299/150‑1 (MQ=255)

                                                                                                                                         gCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCt              <  6:398776/151‑1 (MQ=255)

                                                                                                                                          cataaCTCTTTGCGGCGGTTTCCACCAATGCGATAGGCGTTGGCGAGTTTGGTATTGATAGTGCCATCATGTTCGAGTTCTGGGAGTGGGTTGGCGGGCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTGCATCGGCtt             <  4:1458633/148‑1 (MQ=255)

                                                                                                                                          cAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCtt             >  1:1610331/1‑151 (MQ=255)

                                                                                                                                           aaaaCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCttt            >  2:740396/1‑151 (MQ=255)

                                                                                                                                           aaaaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCttt            >  4:1954385/1‑151 (MQ=255)

                                                                                                                                            aaaCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTg           >  6:883415/1‑151 (MQ=255)

                                                                                                                                               cacTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTATGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGGTTTGAt         >  2:373767/1‑150 (MQ=255)

                                                                                                                                                  tttGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACt     >  7:1306112/1‑151 (MQ=255)

                                                                                                                                                   ttGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTGCGAGTTCTGGGACTGGGGTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACt     <  2:1983515/150‑1 (MQ=255)

AAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCG  >  NC_000913/4234329‑4234628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`