Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913312,8650GA48.2% 109.4 / inf 531intergenic (‑88/+276)ykgM/ykgR50S ribosomal protein L31 type B; alternative zinc‑limitation L31 protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (153/122);  new base A (152/104);  total (305/226)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-02

Only 100 of 584 total aligned reads displayed.

CTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAAT  >  NC_000913/312715‑313015
                                                                                                                                                      |                                                                                                                                                      
atcatcaACCGTGGTTTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTGATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtgg                                                                                                                                                        <  7:1336789/150‑1 (MQ=255)
  catcaACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggat                                                                                                                                                      <  6:1638140/151‑1 (MQ=255)
    tcaACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggatgt                                                                                                                                                    >  4:203856/1‑151 (MQ=255)
       acacTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggatgtgga                                                                                                                                                 <  3:213703/151‑1 (MQ=255)
       acacTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAAACATAAAGATACATGCTCATACGGATCATTTCAACACTAAATCACTGTGACTCTTtgtgtatgtgg                                                                                                                                                  >  3:1056766/1‑150 (MQ=255)
        cacTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtgaatgtgga                                                                                                                                                 >  1:1826105/1‑150 (MQ=255)
         acTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAAt                                                                                                                                               >  3:788768/1‑151 (MQ=255)
         acTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAAt                                                                                                                                               >  6:1069064/1‑151 (MQ=255)
            ggTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAAttt                                                                                                                                             <  1:1188669/150‑1 (MQ=255)
              tgtCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCg                                                                                                                                          >  8:1346966/1‑151 (MQ=255)
                  gTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAAc                                                                                                                                      <  1:648787/151‑1 (MQ=255)
                  gTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAAc                                                                                                                                      <  4:1922237/151‑1 (MQ=255)
                    ggAACACCACAGTACGATACTCTGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACg                                                                                                                                     <  1:36846/150‑1 (MQ=255)
                         accacAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAAc                                                                                                                               >  5:1094293/1‑151 (MQ=255)
                           cacaGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAActc                                                                                                                             >  3:1662874/1‑151 (MQ=255)
                             caGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTg                                                                                                                           <  7:170083/151‑1 (MQ=255)
                             caGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTg                                                                                                                           <  8:741517/151‑1 (MQ=255)
                             caGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTg                                                                                                                           >  4:145277/1‑151 (MQ=255)
                               gTACGATACTCAGGATTGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAAGCATAATGATACATGCTCATCCGGATCAGTTCAACACTCAGCCCCTGTGACTCTGGGGGGAGGTGGAATTTTCGCAACTGGGAACTCTggg                                                                                                                         >  3:679089/1‑150 (MQ=255)
                                  cGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCAtt                                                                                                                      >  6:2012789/1‑151 (MQ=255)
                                      aCTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTa                                                                                                                  <  7:382860/151‑1 (MQ=255)
                                         cAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTag                                                                                                                 <  7:506512/149‑1 (MQ=255)
                                         aaGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTagca                                                                                                               <  6:337651/150‑1 (MQ=255)
                                             agggatATTGGGCTTCAACAATTTTACCTGATATGTTATAACATAACCATAAAGATACATGCTAATCCGGATCATTTCAACAGTCAATCAATGTGACTCTTTGTGAATGTGGAAATTTCGAAACAGGGAAATCTGGCATAGTAagaagca                                                                                                            >  7:1794353/3‑149 (MQ=255)
                                              tggatATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCAt                                                                                                          >  3:549852/1‑151 (MQ=255)
                                              tggatATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCAt                                                                                                          >  4:1152790/1‑151 (MQ=255)
                                               ggatATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCAtt                                                                                                         <  2:1768208/151‑1 (MQ=255)
                                                gatATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTg                                                                                                        >  1:1695799/1‑151 (MQ=255)
                                                   atTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGt                                                                                                      <  6:1858663/150‑1 (MQ=255)
                                                   atTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTg                                                                                                     >  8:569954/1‑151 (MQ=255)
                                                    ttGGGATTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGCATCATTTCAACAATAAATCCATGTGAATATTTGTGGATGTGGAATTTTCGAAACGCGTAAATCTGGCATTGTTAGCAGACATTCGTg                                                                                                     >  3:1115997/1‑150 (MQ=255)
                                                      gggCTTCATGATTTTTCCGTGTTATGTTATAACATAACCATAAAGATACTTGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACg                                                                                                  <  6:1216361/151‑1 (MQ=255)
                                                       ggCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGc                                                                                                 >  4:751686/1‑151 (MQ=255)
                                                          ttCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGTAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTcc                                                                                              >  2:649248/1‑151 (MQ=255)
                                                             atcatTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCAt                                                                                           >  1:1955872/1‑151 (MQ=255)
                                                               catTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATa                                                                                          >  5:1199912/1‑150 (MQ=255)
                                                               catTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCGTa                                                                                          <  2:1094724/150‑1 (MQ=255)
                                                               catTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTCACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATa                                                                                          >  4:1737946/1‑150 (MQ=255)
                                                                  ttttACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACg                                                                                      >  3:1728396/1‑151 (MQ=255)
                                                                   tttACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACg                                                                                      <  8:1099714/150‑1 (MQ=255)
                                                                   tttACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGc                                                                                     >  1:572734/1‑151 (MQ=255)
                                                                    ttACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGc                                                                                     <  5:770895/150‑1 (MQ=255)
                                                                    ttACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGcc                                                                                    >  8:1773490/1‑151 (MQ=255)
                                                                    ttACCTCTTATGTTATAACATACCCATAAAGATACAGGCTCATCCGGATCAATTCAACACTCAATCCCTGTGACTCTTTGTGGAGCTGGAATTTTCCCAACGTGGAACTCTGCCATTGTTAGCAGCCATGGGTGACCCTCCCAGAAACGcc                                                                                    >  1:1981145/1‑151 (MQ=255)
                                                                      aCCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCt                                                                                   <  8:1934274/150‑1 (MQ=255)
                                                                       ccTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTgg                                                                                 >  5:595275/1‑151 (MQ=255)
                                                                       ccTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTgg                                                                                 <  4:493507/151‑1 (MQ=255)
                                                                           ttatgttatAACATAACCATAAAGATACATGATCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGAATTGTTAGCAGCCATTGGTGACGATCCCATAAACGCCTCGATg                                                                              >  2:26899/1‑150 (MQ=255)
                                                                               gttatAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGtg                                                                          >  8:1634757/1‑150 (MQ=255)
                                                                                  ataacataacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGtgattg                                                                      >  7:108568/1‑151 (MQ=255)
                                                                                    aacataacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGTGCAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGtgattgat                                                                    >  2:1366618/1‑151 (MQ=255)
                                                                                    aacataacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGtgattgat                                                                    >  2:1942714/1‑151 (MQ=255)
                                                                                     acataacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATtgatg                                                                   <  7:1204183/151‑1 (MQ=255)
                                                                                       ataacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTgatgat                                                                 <  5:1067228/151‑1 (MQ=255)
                                                                                       ataacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTgatgat                                                                 >  2:481918/1‑151 (MQ=255)
                                                                                         aacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATgg                                                               <  8:1901417/151‑1 (MQ=255)
                                                                                         aacCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGTAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGatgatg                                                                <  1:1773884/150‑1 (MQ=255)
                                                                                           ccATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGtt                                                             <  1:1017024/151‑1 (MQ=255)
                                                                                            cATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGttt                                                            >  8:465822/1‑151 (MQ=255)
                                                                                            cATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGttt                                                            >  3:1941505/1‑151 (MQ=255)
                                                                                            cATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGttt                                                            >  8:1519017/1‑151 (MQ=255)
                                                                                                   aTACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTg                                                       >  1:994275/1‑149 (MQ=255)
                                                                                                     aCATGCTCATCCTGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGagag                                                   <  7:150507/151‑1 (MQ=255)
                                                                                                     aCATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTgaga                                                    <  1:816626/150‑1 (MQ=255)
                                                                                                         gCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATg                                               >  5:902574/1‑151 (MQ=255)
                                                                                                         gCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATg                                               >  3:887540/1‑151 (MQ=255)
                                                                                                             aTCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGAtt                                           <  6:698580/151‑1 (MQ=255)
                                                                                                             aTCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGAtt                                           >  5:213070/1‑151 (MQ=255)
                                                                                                              tCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGAttt                                          >  3:416685/1‑151 (MQ=255)
                                                                                                              tCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATAGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGAttt                                          >  7:1139451/1‑151 (MQ=255)
                                                                                                               ccGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGAtttt                                         >  5:1790608/1‑151 (MQ=255)
                                                                                                               ccGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGAttt                                          <  7:1896453/150‑1 (MQ=255)
                                                                                                                cGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGAttttt                                        <  3:1424775/151‑1 (MQ=255)
                                                                                                                 ggATCATATCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTc                                       <  6:1542223/151‑1 (MQ=255)
                                                                                                                  gATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCg                                      <  2:1473208/151‑1 (MQ=255)
                                                                                                                   aTCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCg                                      >  4:1271829/1‑150 (MQ=255)
                                                                                                                   aTCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCga                                     <  5:137166/151‑1 (MQ=255)
                                                                                                                    tCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCg                                      >  2:763787/1‑149 (MQ=255)
                                                                                                                     cATTTGAACACTCTATCCCTGTGACTGTTTGTGGATGTGGTATTTTCGGAACGGGTAAGTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGATAGTATGGATTTTTCtaga                                   <  6:1353281/151‑1 (MQ=255)
                                                                                                                     cATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCg                                      >  8:1252041/1‑148 (MQ=255)
                                                                                                                        ttCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATg                                >  4:605854/1‑151 (MQ=255)
                                                                                                                          cAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATgg                               >  2:363293/1‑150 (MQ=255)
                                                                                                                          cAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGa                              <  7:879100/151‑1 (MQ=255)
                                                                                                                            acacTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGAAGGTTTTGCTGAGAGTATGGATTTTTCGAGAATCGAgg                            >  7:1974075/1‑151 (MQ=255)
                                                                                                                             cacTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGt                           >  5:1681963/1‑151 (MQ=255)
                                                                                                                             cacTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGt                           <  2:90630/151‑1 (MQ=255)
                                                                                                                              acTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGtt                          >  8:861033/1‑151 (MQ=255)
                                                                                                                              aatCAATCCGTGTTAATCTTTGTGAATGGGGAATTATCGCAACGGGCAACTGTGGCATTTTTAGCAGCCATTGGTGTCGCTCCCTTAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGtt                          <  6:1457381/149‑1 (MQ=255)
                                                                                                                                  aaTCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTAtct                      >  2:1595060/1‑151 (MQ=255)
                                                                                                                                       cTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACt                 >  1:751740/1‑151 (MQ=255)
                                                                                                                                         gtgACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTCTCTCCACtt                <  4:1358761/150‑1 (MQ=255)
                                                                                                                                           gACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTAc             >  5:439842/1‑151 (MQ=255)
                                                                                                                                           gACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTAc             <  6:867071/151‑1 (MQ=255)
                                                                                                                                             ctctTTGTGAATGTGGAATTTTCTCAACGGGGAACTCTGGGATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTg           >  1:1407251/1‑151 (MQ=255)
                                                                                                                                               ctTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTg           <  2:1186739/149‑1 (MQ=255)
                                                                                                                                                tttGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATAGGTTACGCTCCCATAAACGCCTCGATGAGTGATTGATGATGCTTTTGCTGAGAGTATTGATTTTTCGAGAATGGAGGTTATCAACACTTTACTGGcc        >  3:1323087/1‑151 (MQ=255)
                                                                                                                                                 ttGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCg       >  3:1838961/1‑151 (MQ=255)
                                                                                                                                                   gtgaatgtggaATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGAt     >  7:679135/1‑151 (MQ=255)
                                                                                                                                                     ggatttggaATTTTCGCAACGGGGAACTCTTGGATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGATTATGGATTTTTAGAGAATGGAGGTTATCTCCACTTTACTGGCCGATa    <  3:26860/150‑1 (MQ=255)
                                                                                                                                                     ggatgtggaATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTTCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATaa   <  5:1909432/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAAT  >  NC_000913/312715‑313015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑