BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,234,479	0	C	T	53.8%	114.2 / inf	620	A241V (GCG→GTG)	pgi	glucosephosphate isomerase
Reads supporting (aligned to +/- strand): ref base C (158/127); new base T (166/166); total (326/294)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 642 total aligned reads displayed.

AAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGT  >  NC_000913/4234329‑4234629

 aGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgcg                                                                                                                                                       <  3:1308536/151‑1 (MQ=255)

  gTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgtgg                                                                                                                                                      <  8:262087/151‑1 (MQ=255)

   tGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgtggc                                                                                                                                                     >  6:999170/1‑151 (MQ=255)

   tGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgcggc                                                                                                                                                     >  1:1005600/1‑151 (MQ=255)

    gCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgtggc                                                                                                                                                     <  7:438049/150‑1 (MQ=255)

     cTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGgcgc                                                                                                                                                   >  8:1138776/1‑151 (MQ=255)

       gAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCtt                                                                                                                                                 >  4:871809/1‑151 (MQ=255)

       gAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCtt                                                                                                                                                 >  2:1750054/1‑151 (MQ=255)

       gAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCt                                                                                                                                                  <  2:797890/150‑1 (MQ=255)

        aaaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAACCACGTT‑CAAAACACTTTGTGGCGCTTTc                                                                                                                                               >  4:1580982/1‑151 (MQ=255)

           aaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTcca                                                                                                                                             <  1:1986541/151‑1 (MQ=255)

            aaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTccac                                                                                                                                            >  1:2163674/1‑151 (MQ=255)

             aGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTccacc                                                                                                                                           >  8:630008/1‑151 (MQ=255)

                 aaCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATg                                                                                                                                       <  6:1988317/151‑1 (MQ=255)

                      ggAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCaa                                                                                                                                   >  3:230265/1‑150 (MQ=255)

                      ggAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                                                                                                   >  1:551395/1‑150 (MQ=255)

                       gAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAg                                                                                                                                 <  4:1809488/151‑1 (MQ=255)

                         aaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGcc                                                                                                                               <  1:310022/151‑1 (MQ=255)

                         aaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACAAATGCCAAAGcc                                                                                                                               >  1:807744/1‑151 (MQ=255)

                              cGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                                                                                                          <  1:1611275/151‑1 (MQ=255)

                               gCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                                         >  6:1293446/1‑151 (MQ=255)

                               gCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                                         >  6:477913/1‑151 (MQ=255)

                               gCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                                         >  4:944564/1‑151 (MQ=255)

                               gCTGTTCTTGGTAGCATCTAAAACCTACACCACTCAGGAAACTATGACCACCGCCCATAGCGCGCGTGACTGGTTCCTCAAAGCGGCAGGTCATGAAAAACACGTTGCAAAACACTATGTGGCGCTTTCCACCAATGCCAAAGCCGTAGGc                                                                                                                         >  7:529661/1‑151 (MQ=255)

                                  gTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                                                                                                        >  2:490585/1‑149 (MQ=255)

                                  gTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                                                                                                        >  8:1961208/1‑149 (MQ=255)

                                     gttGGTAGGATCTAAACCCTTCACCACTCAGGAAAGTATGACCACCGCCCTTAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttt                                                                                                                   <  5:660883/150‑1 (MQ=255)

                                      ttGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                                                                                                  >  3:2039114/1‑151 (MQ=255)

                                       tGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttt                                                                                                                   <  8:855962/149‑1 (MQ=255)

                                       tGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTgg                                                                                                                 >  5:350898/1‑151 (MQ=255)

                                           aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                                              <  1:1768022/150‑1 (MQ=255)

                                           aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAtt                                                                                                             >  4:2120551/1‑151 (MQ=255)

                                             cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTg                                                                                                            >  1:1169287/1‑150 (MQ=255)

                                                cTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                                                                                                        >  2:1569447/1‑151 (MQ=255)

                                                 aaaaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACt                                                                                                       <  8:2098416/150‑1 (MQ=255)

                                                  aaaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTg                                                                                                      >  7:623003/1‑151 (MQ=255)

                                                  aaaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACt                                                                                                       <  6:1552239/150‑1 (MQ=255)

                                                    aaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGcc                                                                                                    >  5:2172030/1‑151 (MQ=255)

                                                      ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCa                                                                                                   >  4:284633/1‑150 (MQ=255)

                                                         tCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACa                                                                                                <  2:187818/150‑1 (MQ=255)

                                                          caccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAAc                                                                                                 <  4:1178537/148‑1 (MQ=255)

                                                             cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc                                                                                           <  2:1137822/151‑1 (MQ=255)

                                                             cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc                                                                                           <  4:650650/151‑1 (MQ=255)

                                                                 cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg                                                                                        <  2:1249706/150‑1 (MQ=255)

                                                                 cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGt                                                                                       <  6:2067681/151‑1 (MQ=255)

                                                                      aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgg                                                                                  <  2:299407/151‑1 (MQ=255)

                                                                      aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgg                                                                                  <  5:935850/151‑1 (MQ=255)

                                                                          aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACt                                                                              <  1:2095572/151‑1 (MQ=255)

                                                                            gACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTg                                                                             <  7:751338/150‑1 (MQ=255)

                                                                              ccAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                                          <  1:917180/151‑1 (MQ=255)

                                                                              ccAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                                          <  6:37817/151‑1 (MQ=255)

                                                                               cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGtt                                                                         >  4:1093622/1‑151 (MQ=255)

                                                                               cAACCCCCATACCGCGGGGGACTGGATCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAACACAGTTTCTGGCGCTGTCCACCCATGCCAAAGCCGTTGGCGAGGTTGGTACTGATACTGCCCACATGTTCGAGTCCTGGGACGGGGtt                                                                         <  4:1960634/151‑1 (MQ=255)

                                                                                         aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGtt                                                               >  3:1141983/1‑151 (MQ=255)

                                                                                           cgcgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAc                                                             >  2:1443755/1‑151 (MQ=255)

                                                                                            gcgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAct                                                            <  1:969489/151‑1 (MQ=255)

                                                                                              gcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTActct                                                          >  2:914137/1‑151 (MQ=255)

                                                                                               cgTGACTGGTTCCTGAATGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCtt                                                         <  5:1441824/151‑1 (MQ=255)

                                                                                                   aCTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtgtg                                                     <  6:1429860/151‑1 (MQ=255)

                                                                                                     tGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGt                                                   >  7:1362204/1‑151 (MQ=255)

                                                                                                      ggTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                                  >  3:843240/1‑151 (MQ=255)

                                                                                                      ggTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                                  <  3:578291/151‑1 (MQ=255)

                                                                                                       gTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                                  >  7:319662/1‑150 (MQ=255)

                                                                                                       gTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                                  >  8:564563/1‑150 (MQ=255)

                                                                                                       gTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCa                                                 <  8:1774810/151‑1 (MQ=255)

                                                                                                        ttCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTctg                                                >  8:1249778/1‑149 (MQ=255)

                                                                                                        ttCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                                  >  5:1109099/1‑149 (MQ=255)

                                                                                                          ccTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCg                                              >  6:721106/1‑151 (MQ=255)

                                                                                                          ccTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCg                                              <  1:1309236/151‑1 (MQ=255)

                                                                                                               aaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTgg                                         >  6:1730734/1‑151 (MQ=255)

                                                                                                                   ggCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                     <  8:350175/151‑1 (MQ=255)

                                                                                                                   ggCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                     >  3:1522815/1‑151 (MQ=255)

                                                                                                                        gTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt                                <  7:160003/151‑1 (MQ=255)

                                                                                                                           atgaAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGt                             >  7:843724/1‑151 (MQ=255)

                                                                                                                            tgaAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGtt                            >  8:1407409/1‑151 (MQ=255)

                                                                                                                             gaAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGGCAGCGATTGGCCTGTCGATTGtt                            <  7:1442192/150‑1 (MQ=255)

                                                                                                                             gaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtc                           >  2:2067581/1‑151 (MQ=255)

                                                                                                                                aaaCACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTATtctct                        <  7:1946474/151‑1 (MQ=255)

                                                                                                                                 aaCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctctc                       <  5:1354483/151‑1 (MQ=255)

                                                                                                                                 aaCACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctctc                       >  8:749033/1‑151 (MQ=255)

                                                                                                                                   cacGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTcc                      >  5:1739481/1‑150 (MQ=255)

                                                                                                                                    acGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAt                    <  7:1745358/151‑1 (MQ=255)

                                                                                                                                    acGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAt                    >  3:1878896/1‑151 (MQ=255)

                                                                                                                                     ggTTGCAAAACACTTTGCGGCGCTTTCCACCAATGGCAAAGCGGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                   <  7:1118268/150‑1 (MQ=255)

                                                                                                                                     cGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGGCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                   <  3:1615240/151‑1 (MQ=255)

                                                                                                                                      gTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCg                  <  3:1004786/151‑1 (MQ=255)

                                                                                                                                        tGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGc                >  1:429494/1‑151 (MQ=255)

                                                                                                                                        tGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGACTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTCGTCAGCGATAGGCCTGTCGATTCTTCTCTCCAACGGc                >  7:95624/1‑151 (MQ=255)

                                                                                                                                           aaatCACTTTGCGGGGGTTTCCAGCTTTGCGATTGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGGCGTTACTCTTTGTGGTCAGCGATTGGGCTGTCGATTGTTCTCTCCATCGGCttt             <  5:788431/151‑1 (MQ=255)

                                                                                                                                           aaaaCAGTTTGTGGCGCTTTCCACCAATGCCTAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACTTGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCttt             <  6:1032006/151‑1 (MQ=255)

                                                                                                                                            aaaCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTg            <  7:231421/151‑1 (MQ=255)

                                                                                                                                             aaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTg            >  6:887918/1‑150 (MQ=255)

                                                                                                                                              acacTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGAt          >  6:229084/1‑151 (MQ=255)

                                                                                                                                              acacTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGAt          >  8:1552399/1‑151 (MQ=255)

                                                                                                                                               cacTTTGCGGCGCTGTCCACCAATGCCAAAGCCGGTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATa         <  5:1201659/151‑1 (MQ=255)

                                                                                                                                                acTTTGCGGCGCTTTCCACCAATGCCTAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATa         >  8:1684103/1‑150 (MQ=255)

                                                                                                                                                acTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTCGCGAGTTTGCTATTGATACTGCCAACATGTTAGAGTTCTGGGACTTGGTTGGCGGCCGTTACTCATTGTGGTCAGCGATTGGCCTGTCGATTGATCTCTCCATCGGCTTTGATa         >  4:735043/1‑150 (MQ=255)

                                                                                                                                                  tttGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTACCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACt      >  2:676847/1‑151 (MQ=255)

                                                                                                                                                   ttGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACt      <  5:158774/150‑1 (MQ=255)

                                                                                                                                                     gcggcgCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCATCATGTTCGAGTTCTGGGACTGGGTTGGCGTCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCg   <  4:1328953/151‑1 (MQ=255)

AAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGT  >  NC_000913/4234329‑4234629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`