Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,189,8080GT49.6% 114.9 / inf 365R214S (CGC→AGC) phoPresponse regulator in two‑component regulatory system with PhoQ
Reads supporting (aligned to +/- strand):  ref base G (98/86);  new base T (99/82);  total (197/168)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 371 total aligned reads displayed.

AACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATT  >  NC_000913/1189659‑1189958
                                                                                                                                                     |                                                                                                                                                      
aaCCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCcgcg                                                                                                                                                        <  2:1699865/150‑1 (MQ=255)
aaCCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGa                                                                                                                                                       <  3:85132/151‑1 (MQ=255)
 aCCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGa                                                                                                                                                       >  6:718456/1‑150 (MQ=255)
  ccGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAAc                                                                                                                                                     <  5:1448930/151‑1 (MQ=255)
    gATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGAGAACgg                                                                                                                                                   >  8:303234/1‑151 (MQ=255)
      tCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGACAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACggtg                                                                                                                                                 >  2:64020/1‑151 (MQ=255)
       cAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACggtgg                                                                                                                                                <  5:395805/151‑1 (MQ=255)
        aGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACggtggt                                                                                                                                               >  2:754999/1‑151 (MQ=255)
         gcgcGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACggtggt                                                                                                                                               >  5:480688/1‑150 (MQ=255)
          cgcgACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGTCGCTAACGGTGGTaa                                                                                                                                             <  5:1579987/151‑1 (MQ=255)
            cgACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATc                                                                                                                                           >  5:1349836/1‑151 (MQ=255)
              aCCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCAc                                                                                                                                         <  2:371896/151‑1 (MQ=255)
                cATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActt                                                                                                                                       >  4:715590/1‑151 (MQ=255)
                cATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActt                                                                                                                                       <  2:1637933/151‑1 (MQ=255)
                   tCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCActtctt                                                                                                                                    >  4:945809/1‑151 (MQ=255)
                    ccGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtctt                                                                                                                                    <  5:1048428/150‑1 (MQ=255)
                     cGTAGGCAAGCGAAAGCACCAGTACTACCGCTCCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTgg                                                                                                                                  >  3:528626/1‑151 (MQ=255)
                      gTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTggg                                                                                                                                 >  7:372986/1‑151 (MQ=255)
                      gTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTggg                                                                                                                                 >  7:597718/1‑151 (MQ=255)
                        aGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTgggg                                                                                                                                >  8:643197/1‑150 (MQ=255)
                        aGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGa                                                                                                                               <  3:1235724/151‑1 (MQ=255)
                           cAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGatat                                                                                                                            >  8:349476/1‑151 (MQ=255)
                           cAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGatat                                                                                                                            <  7:1410548/151‑1 (MQ=255)
                            aaGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCAGTTCTTGGGGATAtt                                                                                                                           >  7:503799/1‑151 (MQ=255)
                               cGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATtgtg                                                                                                                        >  4:573632/1‑151 (MQ=255)
                                gAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGc                                                                                                                       >  3:1388710/1‑151 (MQ=255)
                                gAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGc                                                                                                                       >  4:1379472/1‑151 (MQ=255)
                                gAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGc                                                                                                                       >  7:765149/1‑151 (MQ=255)
                                   aGCACCAGTTCTTCCTGTGCCGTTTCCATCAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTTATCACTTCTTGGGGATATTGTGCCtt                                                                                                                    <  3:715492/151‑2 (MQ=255)
                                         aGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttt                                                                                                              <  8:1755984/151‑1 (MQ=255)
                                          gTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAttttt                                                                                                             <  4:372128/151‑1 (MQ=255)
                                          gTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAA‑TTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAtttttc                                                                                                            <  2:1722088/151‑2 (MQ=255)
                                              tacCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgc                                                                                                         >  5:747917/1‑151 (MQ=255)
                                               acCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgc                                                                                                         <  5:638363/150‑1 (MQ=255)
                                                ccGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGAGCCTGAATTTTTTTgcgc                                                                                                       >  2:893570/1‑151 (MQ=255)
                                                 cGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgagc                                                                                                       >  6:896268/1‑150 (MQ=255)
                                                 cGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCa                                                                                                      <  3:474582/151‑1 (MQ=255)
                                                   cTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTgcgc                                                                                                       >  2:1501916/1‑148 (MQ=255)
                                                    tGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGAc                                                                                                   >  7:1122110/1‑151 (MQ=255)
                                                      ccGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGt                                                                                                 <  4:1125227/151‑1 (MQ=255)
                                                        gTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTcc                                                                                               >  7:421976/1‑151 (MQ=255)
                                                                cAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGt                                                                                        >  8:906873/1‑150 (MQ=255)
                                                                     aaCGTAACCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTACCGAAGACGTCCCATCAGTACATc                                                                                   >  4:832842/1‑150 (MQ=255)
                                                                      aCGTACACGCAGCGAGAGCGGGAAAAAAAGAGGCAGTAATTTTTTGATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTACTTGGGGATATTGTGCCTGAATTTTTTTGCGCCGACGTCCCTTCAGTAcgtga                                                                                  >  6:1078755/1‑146 (MQ=255)
                                                                        gTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATg                                                                               >  1:599735/1‑151 (MQ=255)
                                                                          aCCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGt                                                                             <  1:1756585/151‑1 (MQ=255)
                                                                           cccGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAAATCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTa                                                                            <  6:1432398/151‑1 (MQ=255)
                                                                            ccGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTAt                                                                           >  7:898895/1‑151 (MQ=255)
                                                                            ccGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTAt                                                                           <  5:1638975/151‑1 (MQ=255)
                                                                             cGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATg                                                                          >  1:1300191/1‑151 (MQ=255)
                                                                                  cGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCttt                                                                     >  6:382867/1‑151 (MQ=255)
                                                                                   gagaGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTc                                                                    >  4:1692676/1‑151 (MQ=255)
                                                                                        cGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCa                                                               <  8:1222258/151‑1 (MQ=255)
                                                                                        cGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCa                                                               <  5:1178734/151‑1 (MQ=255)
                                                                                             aaaaaaGACGCAGTAATTTTTTCATCAGCGCAATTCGAACTGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGCTATGGCTTTCCCGCAGCTcc                                                          >  3:1141646/1‑151 (MQ=255)
                                                                                                aaaGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGc                                                        >  4:1532708/1‑150 (MQ=255)
                                                                                                aaaGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGc                                                        >  8:785873/1‑150 (MQ=255)
                                                                                                 aaGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGTACGGTGGTAATGACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCAt                                                      <  7:686519/151‑1 (MQ=255)
                                                                                                      ccAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTACGCATCCGGa                                                 <  8:458695/150‑1 (MQ=255)
                                                                                                        aGTTATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATGACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTGCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATa                                               <  8:1422000/151‑1 (MQ=255)
                                                                                                          tAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACCGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCACATCAGTACATCAATGGTATCGCTTTCCCGCAGCTCCGCATcagtat                                                >  8:1074355/1‑143 (MQ=255)
                                                                                                           aaTTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATagag                                            <  1:1010908/151‑1 (MQ=255)
                                                                                                             ttttttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGtt                                          >  2:998760/1‑151 (MQ=255)
                                                                                                              tttttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTg                                         >  8:547946/1‑151 (MQ=255)
                                                                                                              tttttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTg                                         >  1:487195/1‑151 (MQ=255)
                                                                                                              tttttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTg                                         >  7:1707428/1‑151 (MQ=255)
                                                                                                               ttttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTACCGCAGCTCCGCATCCGGATAGAGTTgg                                        >  4:1372377/1‑151 (MQ=255)
                                                                                                                 ttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTCGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTgg                                        >  7:1268006/1‑149 (MQ=255)
                                                                                                                    atcaGCGCAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCAt                                   <  1:1124509/151‑1 (MQ=255)
                                                                                                                         cgcAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACg                              <  1:874232/151‑1 (MQ=255)
                                                                                                                         cgcAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACg                              <  3:1548415/151‑1 (MQ=255)
                                                                                                                          gcAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACg                              >  2:1030270/1‑150 (MQ=255)
                                                                                                                           cAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCGCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGa                             >  1:1562869/1‑150 (MQ=255)
                                                                                                                           cAATTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGaa                            <  1:373687/151‑1 (MQ=255)
                                                                                                                            aaTTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAAt                           >  3:983328/1‑151 (MQ=255)
                                                                                                                            aaTTCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAAt                           <  7:924243/151‑1 (MQ=255)
                                                                                                                              ttCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCt                         >  3:218219/1‑151 (MQ=255)
                                                                                                                              ttCGAACAGATAGCCCTGGCCGCTAAAGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTAGATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCt                         >  6:1231743/1‑151 (MQ=255)
                                                                                                                              ttCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCt                         >  5:188346/1‑151 (MQ=255)
                                                                                                                              ttCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCt                         >  2:1601798/1‑151 (MQ=255)
                                                                                                                               tCGAACAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCtt                        >  1:948127/1‑151 (MQ=255)
                                                                                                                                cgtaCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCttt                       <  6:245933/148‑1 (MQ=255)
                                                                                                                                cGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCttt                       <  7:1514011/151‑1 (MQ=255)
                                                                                                                                cGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCttt                       >  8:1656742/1‑151 (MQ=255)
                                                                                                                                 gAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTg                      <  2:253016/151‑1 (MQ=255)
                                                                                                                                 gAACAGATAGCCCTGGACGCGAACGGTGGTAAACACTACTTGGGGATATTGTGCCTGAATTTTTTTGCCCAGACGTCCCATCAGTACATCAATGGAATCGCTTACCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAAACtt                        >  1:260911/1‑149 (MQ=255)
                                                                                                                                  aaCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGc                     >  4:130009/1‑151 (MQ=255)
                                                                                                                                   aCAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCt                    <  1:717820/151‑1 (MQ=255)
                                                                                                                                   aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCt                    <  5:962077/151‑1 (MQ=255)
                                                                                                                                   aCAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGAGAGAGTTGGAGCATTAGCGAATCTTTGCt                    >  3:1316624/1‑151 (MQ=255)
                                                                                                                                    cAGATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTg                   >  6:15341/1‑151 (MQ=255)
                                                                                                                                      gATAGCCCTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGAc                 >  4:273321/1‑151 (MQ=255)
                                                                                                                                        tAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGAcc                >  2:473131/1‑150 (MQ=255)
                                                                                                                                         aGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCAc              >  2:1341261/1‑151 (MQ=255)
                                                                                                                                          gCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTCCATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACt             >  7:97631/1‑151 (MQ=255)
                                                                                                                                           cccTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACtt            >  6:232857/1‑151 (MQ=255)
                                                                                                                                            ccTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACttt           >  6:1175882/1‑151 (MQ=255)
                                                                                                                                            ccTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACttt           >  2:1185468/1‑151 (MQ=255)
                                                                                                                                             cTGGCCGCTAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTg          >  3:352119/1‑151 (MQ=255)
                                                                                                                                                gCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCa       <  6:490995/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATT  >  NC_000913/1189659‑1189958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑