Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,213,4580CA50.1% 87.1 / inf 355R396L (CGC→CTC) yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (90/87);  new base A (89/89);  total (179/176)
Fisher's exact test for biased strand distribution p-value = 9.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

GACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATC  >  NC_000913/2213308‑2213607
                                                                                                                                                      |                                                                                                                                                     
    ggAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGcggcggct                                                                                                                                                   >  3:1040209/1‑149 (MQ=255)
    ggAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGcggcgg                                                                                                                                                     <  7:1589836/149‑1 (MQ=255)
         cgcgcCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCg                                                                                                                                              >  3:782381/1‑151 (MQ=255)
           cgcCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCt                                                                                                                                            <  5:367846/151‑1 (MQ=255)
            gcCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCtt                                                                                                                                           >  2:549758/1‑151 (MQ=255)
            gcCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCtt                                                                                                                                           >  6:1401630/1‑151 (MQ=255)
            gcCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCtt                                                                                                                                           >  6:609636/1‑151 (MQ=255)
            gcCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTGTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCtt                                                                                                                                           >  3:16305/1‑151 (MQ=255)
             ccTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCttt                                                                                                                                          <  4:221861/151‑1 (MQ=255)
             ccTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCttt                                                                                                                                          <  2:1649045/151‑1 (MQ=255)
                 ttCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATg                                                                                                                                      >  6:867672/1‑151 (MQ=255)
                  tCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATgg                                                                                                                                     >  8:1484855/1‑151 (MQ=255)
                         gCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTa                                                                                                                                >  5:1356729/1‑149 (MQ=255)
                         gCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAg                                                                                                                              <  7:241777/151‑1 (MQ=255)
                          cAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAgc                                                                                                                             <  2:425089/151‑1 (MQ=255)
                           aGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAgcg                                                                                                                            >  8:453840/1‑151 (MQ=255)
                           aGATAAGCATGCACATGTTCAATTTCGTCGGAGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAgcg                                                                                                                            <  7:137340/151‑1 (MQ=255)
                             aTAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGGTAAgcgc                                                                                                                           >  8:1306492/1‑150 (MQ=255)
                                 gCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCCCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTaa                                                                                                                      <  4:1155001/151‑1 (MQ=255)
                                   aTTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAAc                                                                                                                    <  7:1701609/151‑1 (MQ=255)
                                   aTTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAAc                                                                                                                    >  8:199016/1‑151 (MQ=255)
                                   aTTCACATGTTCAATTTCGTCGGAGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAACTGGAATGATACTGAACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCTCTTTAATGGTGTTAAGCGCATTAAAc                                                                                                                    <  1:570502/151‑1 (MQ=255)
                                      cacaTGTTCAATTTCGTCGTCGAGAGTAACAAACTCCGAAGGACTCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACaa                                                                                                                  <  2:1765201/150‑1 (MQ=255)
                                      cacaTGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACaaa                                                                                                                 <  1:1321422/151‑1 (MQ=255)
                                       acaTGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACaaaa                                                                                                                <  3:292949/151‑1 (MQ=255)
                                            ttCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCCCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATgg                                                                                                           >  5:1414261/1‑151 (MQ=255)
                                             tCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTTGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATgg                                                                                                           <  4:1243865/150‑1 (MQ=255)
                                              cAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATggg                                                                                                          <  5:673293/150‑1 (MQ=255)
                                                  ttCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTc                                                                                                     >  2:1220513/1‑151 (MQ=255)
                                                     gtcgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCAcc                                                                                                  >  4:1685747/1‑151 (MQ=255)
                                                     gtcgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCAcc                                                                                                  >  7:1148523/1‑151 (MQ=255)
                                                       cgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTg                                                                                                >  8:1085089/1‑151 (MQ=255)
                                                       cgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCt                                                                                                 <  1:230146/150‑1 (MQ=255)
                                                         gCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTggg                                                                                              >  5:1249298/1‑151 (MQ=255)
                                                          cGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGc                                                                                             <  4:813950/151‑1 (MQ=255)
                                                            agagTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCgg                                                                                           >  5:1532207/1‑150 (MQ=255)
                                                               gTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGaa                                                                                        <  5:1709023/151‑1 (MQ=255)
                                                                  aCAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCa                                                                                     <  5:538608/151‑1 (MQ=255)
                                                                  aCAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGc                                                                                      >  4:349458/1‑150 (MQ=255)
                                                                    aaaCTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGt                                                                                   >  5:824664/1‑151 (MQ=255)
                                                                      aCTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGttt                                                                                 <  6:1208939/151‑1 (MQ=255)
                                                                            aaGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGAtctc                                                                           <  3:272981/151‑1 (MQ=255)
                                                                            aaGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGAtctc                                                                           >  8:592596/1‑151 (MQ=255)
                                                                             aGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATctct                                                                          <  4:400821/151‑1 (MQ=255)
                                                                              ggCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTg                                                                         <  2:312383/151‑1 (MQ=255)
                                                                                ccGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGAc                                                                       >  6:1062159/1‑151 (MQ=255)
                                                                                 cGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACt                                                                      <  7:1643231/151‑1 (MQ=255)
                                                                                 cGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACt                                                                      <  7:492857/151‑1 (MQ=255)
                                                                                     ttAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGt                                                                  >  6:1263044/1‑151 (MQ=255)
                                                                                       aaGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCCCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCCCCGGGGCGTGAAGCAGTTTGATCTCTGACTGGGTgc                                                                >  2:756640/1‑150 (MQ=255)
                                                                                       aaGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTg                                                                 >  2:498031/1‑150 (MQ=255)
                                                                                        aGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTg                                                                 >  4:1476421/1‑149 (MQ=255)
                                                                                        aGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAg                                                               <  8:316120/151‑1 (MQ=255)
                                                                                          tttttGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGCTCTCTGACTGGGTGAGc                                                              >  2:1428643/1‑150 (MQ=255)
                                                                                          tttttGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGc                                                              >  6:1749625/1‑150 (MQ=255)
                                                                                           ttttGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCa                                                             <  4:632834/150‑1 (MQ=255)
                                                                                           ttttGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGATGGCATGTTAGCTGTCGCGGAGGATCACCGCTTTAATGCTGTTAAGCGCAATAAACAAAAAATGGGGATTCACCAGGGAGTGAAGCAGTGTGCTCTCTGCCTGGGTGAGcc                                                             >  6:966877/1‑149 (MQ=255)
                                                                                              tGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGAATGGGTGAGCATCGc                                                         >  8:1389739/1‑151 (MQ=255)
                                                                                                 ggAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCttt                                                      <  1:1587898/151‑1 (MQ=255)
                                                                                                   aaaaaaGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCttttt                                                    <  1:143431/151‑1 (MQ=255)
                                                                                                   aaaaaaGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCttttt                                                    <  8:83425/151‑1 (MQ=255)
                                                                                                    aaaaaGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTg                                                   <  4:1576993/151‑1 (MQ=255)
                                                                                                    aaaaaGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTg                                                   >  7:1115270/1‑151 (MQ=255)
                                                                                                     aaaaGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGc                                                  <  7:603733/151‑1 (MQ=255)
                                                                                                         gTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCt                                              >  1:332973/1‑151 (MQ=255)
                                                                                                           ggAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTc                                             <  4:488635/150‑1 (MQ=255)
                                                                                                           ggAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTc                                             <  7:944132/150‑1 (MQ=255)
                                                                                                             aaaGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCata                                          <  1:1150917/151‑1 (MQ=255)
                                                                                                              aaGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCatat                                         <  1:888650/151‑1 (MQ=255)
                                                                                                              aaGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCatat                                         >  3:663875/1‑151 (MQ=255)
                                                                                                               aGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATAtt                                        <  8:934222/151‑1 (MQ=255)
                                                                                                                 aTACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGGAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTgg                                      >  4:847038/1‑150 (MQ=255)
                                                                                                                  tACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGcc                                     >  7:340123/1‑151 (MQ=255)
                                                                                                                  tACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGcc                                     >  8:732490/1‑151 (MQ=255)
                                                                                                                     tGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCgg                                  >  6:1354718/1‑151 (MQ=255)
                                                                                                                      gCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGc                                 <  1:77147/151‑1 (MQ=255)
                                                                                                                      gCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGGGAAGCAGTGTGATCTCTGCCTGGGTTAGCATCGCTTTTTGCGGCTCATATTGCCCgg                                  >  3:1322151/1‑150 (MQ=255)
                                                                                                                      gCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCgg                                  <  7:729033/150‑1 (MQ=255)
                                                                                                                        aCCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCa                                >  3:1478455/1‑150 (MQ=255)
                                                                                                                        aCCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTACCCGGCa                                >  8:535614/1‑150 (MQ=255)
                                                                                                                           aGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCACgg                             >  3:958525/1‑150 (MQ=255)
                                                                                                                           aGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAgg                             >  6:353194/1‑150 (MQ=255)
                                                                                                                           aGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGa                            <  6:514639/151‑1 (MQ=255)
                                                                                                                             ctggctggCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATc                          >  3:1496335/1‑151 (MQ=255)
                                                                                                                              tggctggcCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGGTCATATTGCCCGGCAAGGATCt                         >  6:246175/1‑151 (MQ=255)
                                                                                                                              tggctggcCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCt                         >  5:949551/1‑151 (MQ=255)
                                                                                                                              tggctggcCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGACCGGCAAGGAtat                         >  2:1703408/1‑149 (MQ=255)
                                                                                                                              gggctggcCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCt                         <  1:1224744/150‑1 (MQ=255)
                                                                                                                                gctggcCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGCTCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCACGGATCTgc                       >  1:105340/1‑151 (MQ=255)
                                                                                                                                  tggcCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTgcgc                     >  3:1168413/1‑151 (MQ=255)
                                                                                                                                    gcCTGTTCGCTGTCGCGGAGGATGACCTCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTGACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCg                   <  7:1337170/151‑1 (MQ=255)
                                                                                                                                    gcCTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGTTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCg                   <  3:218230/151‑1 (MQ=255)
                                                                                                                                     ccTGTTCGCTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGa                  <  2:1317792/151‑1 (MQ=255)
                                                                                                                                      cTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGa                  >  7:883798/1‑150 (MQ=255)
                                                                                                                                            gCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAAt           <  2:866439/151‑1 (MQ=255)
                                                                                                                                             cTGTCGCGGAGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAAtt          >  7:1472804/1‑151 (MQ=255)
                                                                                                                                               gTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTgc        <  5:463979/151‑1 (MQ=255)
                                                                                                                                                 cgcgGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTgcgc      >  3:410318/1‑151 (MQ=255)
                                                                                                                                                   cggcggATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCaa    <  8:1613451/151‑1 (MQ=255)
                                                                                                                                                     gcggATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATc  <  8:1162946/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GACTGGAAGCGCGCCTTTTCAATTTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATC  >  NC_000913/2213308‑2213607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑