Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,456,1630CA49.7% 142.1 / inf 512intergenic (‑517/+164)yfcV/sixAputative fimbrial‑like adhesin protein/phosphohistidine phosphatase
Reads supporting (aligned to +/- strand):  ref base C (104/153);  new base A (127/127);  total (232/280)
Fisher's exact test for biased strand distribution p-value = 3.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-02

Only 100 of 520 total aligned reads displayed.

CGATATAATATATAAACACTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGC  >  NC_000913/2456013‑2456311
                                                                                                                                                      |                                                                                                                                                    
   tataatataTAAACACTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGaaa                                                                                                                                                    >  5:1021397/1‑150 (MQ=255)
      aatataTAAACACTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATc                                                                                                                                                <  7:1265981/151‑1 (MQ=255)
       atatatAAACACTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATc                                                                                                                                                >  7:36133/1‑150 (MQ=255)
       atatatAAACACTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCa                                                                                                                                               <  3:1371736/151‑1 (MQ=255)
         ctcttccgatcttaGCCAGAATTTATTCTAGTTTCAATTTCGTCCTGCCTGATTATTCAAGTGTATTTTCTGCATTATGTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATc                                                                                                                                                <  3:313824/136‑1 (MQ=255)
             aaaCACTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAc                                                                                                                                         >  7:502916/1‑151 (MQ=255)
               acacTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATAcgc                                                                                                                                       >  5:384847/1‑151 (MQ=255)
                cacTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATAcgcg                                                                                                                                      >  7:1643777/1‑151 (MQ=255)
                 acTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACgcgc                                                                                                                                     >  8:1034770/1‑151 (MQ=255)
                 acTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACgcgc                                                                                                                                     <  6:603209/151‑1 (MQ=255)
                    ataGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACgcgc                                                                                                                                     >  2:469634/1‑148 (MQ=255)
                    ataGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACgcgc                                                                                                                                     >  3:252023/1‑148 (MQ=255)
                      aGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCagaag                                                                                                                                <  6:1670327/151‑1 (MQ=255)
                      aGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCaga                                                                                                                                  >  4:428394/1‑149 (MQ=255)
                        ccAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAgaagaa                                                                                                                              <  1:1481636/151‑1 (MQ=255)
                        ccAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAgaagaa                                                                                                                              <  4:1094019/151‑1 (MQ=255)
                           gAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATAc                                                                                                                           >  5:463236/1‑151 (MQ=255)
                             aTTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACtt                                                                                                                         <  6:181967/151‑1 (MQ=255)
                             aTTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACtt                                                                                                                         <  1:1764922/151‑1 (MQ=255)
                             aTTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACtt                                                                                                                         <  2:1268502/151‑1 (MQ=255)
                             aTTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACtt                                                                                                                         <  2:1015377/151‑1 (MQ=255)
                                 aaTCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTcc                                                                                                                      <  4:891072/150‑1 (MQ=255)
                                 aaTCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTcc                                                                                                                      <  1:751683/150‑1 (MQ=255)
                                 aaTCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCg                                                                                                                     <  8:1340630/151‑1 (MQ=255)
                                 aaTCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCg                                                                                                                     <  5:718245/151‑1 (MQ=255)
                                  aTCAAGTTTCAATTACTTCCTGACTGATTATTCAACTATATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTAAAACAGACATTATTCATTTAAACATGTAACTTTGGGAAAAATCAAGATACGAGCAGAAGAATACTTTCCg                                                                                                                     >  3:1187786/1‑150 (MQ=255)
                                  aTCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGa                                                                                                                    <  4:677040/151‑1 (MQ=255)
                                  aTCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGa                                                                                                                    <  2:1437555/151‑1 (MQ=255)
                                       gTTTCAATTACGTCCTGCCTGATTATTCAACTGTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGa                                                                                                                <  5:1445689/150‑1 (MQ=255)
                                         ttCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGaaa                                                                                                              <  4:1316213/150‑1 (MQ=255)
                                         ttCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGaa                                                                                                               >  2:275926/1‑149 (MQ=255)
                                          tCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAg                                                                                                             <  2:531567/150‑1 (MQ=255)
                                          tCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGt                                                                                                            >  7:948871/1‑151 (MQ=255)
                                           cAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGt                                                                                                            <  8:182384/150‑1 (MQ=255)
                                           cAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGtt                                                                                                           <  6:394962/151‑1 (MQ=255)
                                           cAATTAAGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATATCTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGCGAAAAATCAAGATACGCGCAGAAGAATACTTTCAGAATGAAAttt                                                                                                           >  7:1522194/1‑151 (MQ=255)
                                            aaTTTCGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTa                                                                                                          <  1:1024540/151‑1 (MQ=255)
                                            aaTTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTa                                                                                                          <  5:205673/151‑1 (MQ=255)
                                            aaTTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGtt                                                                                                           >  4:1740493/1‑150 (MQ=255)
                                            aaTTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTa                                                                                                          <  8:240251/151‑1 (MQ=255)
                                               tACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTaa                                                                                                         >  2:1565022/1‑149 (MQ=255)
                                                aCGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAg                                                                                                       <  1:1108947/150‑1 (MQ=255)
                                                 cGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGgt                                                                                                     <  8:730128/151‑1 (MQ=255)
                                                 cGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAACTTAAAGgt                                                                                                     >  3:1630600/1‑151 (MQ=255)
                                                  gTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGgtg                                                                                                    <  2:980828/151‑1 (MQ=255)
                                                    cctgcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGtt                                                                                                  >  1:1188776/1‑151 (MQ=255)
                                                       gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGtttt                                                                                                <  5:1278520/150‑1 (MQ=255)
                                                       gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGttt                                                                                                 <  6:1275111/149‑1 (MQ=255)
                                                           gATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTtttattta                                                                                           <  6:1049865/151‑1 (MQ=255)
                                                            attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTttatttat                                                                                          >  3:668243/1‑151 (MQ=255)
                                                            attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTttatttat                                                                                          >  1:492387/1‑151 (MQ=255)
                                                             ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTttatttat                                                                                          >  6:1424160/1‑150 (MQ=255)
                                                              tattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTttatttat                                                                                          >  4:1410864/1‑149 (MQ=255)
                                                                  cAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATgg                                                                                    <  8:1228044/151‑1 (MQ=255)
                                                                  cAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATgg                                                                                    >  6:914135/1‑151 (MQ=255)
                                                                    aCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATgggg                                                                                  <  1:59958/151‑1 (MQ=255)
                                                                      tctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATgggg                                                                                  >  8:1576703/1‑149 (MQ=255)
                                                                       ctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATgggg                                                                                  >  8:663386/1‑148 (MQ=255)
                                                                         aTTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGt                                                                             >  6:482113/1‑151 (MQ=255)
                                                                         aTTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTAAAGATGGGgag                                                                                <  2:710594/148‑1 (MQ=255)
                                                                               tcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCg                                                                        >  2:630095/1‑150 (MQ=255)
                                                                                ccATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGAt                                                                      <  5:1114901/151‑1 (MQ=255)
                                                                                  aTTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGAtag                                                                    <  3:1742669/151‑1 (MQ=255)
                                                                                         gTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAg                                                             <  3:486073/151‑1 (MQ=255)
                                                                                            ttAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAAGATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGt                                                           <  1:394143/150‑1 (MQ=255)
                                                                                             taataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGCGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGTGGTTg                                                         >  5:1494774/1‑151 (MQ=255)
                                                                                              aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGTAGAGTCTTCGATAGTAGTGAGGTTg                                                         >  8:311774/1‑150 (MQ=255)
                                                                                              aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTg                                                         >  6:970641/1‑150 (MQ=255)
                                                                                                 aaaTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACa                                                      <  8:478854/150‑1 (MQ=255)
                                                                                                 aaaTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGAc                                                       >  3:922113/1‑149 (MQ=255)
                                                                                                  aaTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGAc                                                       >  6:1555343/1‑148 (MQ=255)
                                                                                                   aTGATCATGAGTGTCAATCATACATTATTCATTTCAAGATGTAACTTTGGGCAAAATCAAGATACGGTCAGAAGAATACTTTCCGAATGAAATTTAAAGGTTTTTTATTTATAGATTGTGAGAGTCTTCGATAGTAGTGAGGTTGACatat                                                   <  4:306503/151‑1 (MQ=255)
                                                                                                   aTGATAATGAGTGGCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGat                                                   <  1:1339329/151‑1 (MQ=255)
                                                                                                     gATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGata                                                  <  7:146861/150‑1 (MQ=255)
                                                                                                     gATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGata                                                  <  2:787211/150‑1 (MQ=255)
                                                                                                      aTAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATc                                                >  7:1174878/1‑151 (MQ=255)
                                                                                                      aTAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATc                                                <  5:1568171/151‑1 (MQ=255)
                                                                                                        aaTGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCg                                              >  4:794081/1‑151 (MQ=255)
                                                                                                          tGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCGGAATGAAAGTTAAAGGTGTTTTATTTATTGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCt                                            >  6:281192/1‑151 (MQ=255)
                                                                                                                 cAAACAGACATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCtgat                                     >  5:639843/1‑151 (MQ=255)
                                                                                                                  aaaGAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCTCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCtgatg                                    <  6:828776/151‑1 (MQ=255)
                                                                                                                  aaaCAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCtgatg                                    >  7:810760/1‑151 (MQ=255)
                                                                                                                  aaaCAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCtgatg                                    >  3:1295026/1‑151 (MQ=255)
                                                                                                                   aaCAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCtgatga                                   <  7:722749/151‑1 (MQ=255)
                                                                                                                     cAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGc                                 >  8:783754/1‑151 (MQ=255)
                                                                                                                         cATTATTCATTTCAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAAtt                             >  6:1588727/1‑151 (MQ=255)
                                                                                                                                aaTTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCa                      <  5:1679698/150‑1 (MQ=255)
                                                                                                                                 aTTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCaa                     <  1:1692369/151‑1 (MQ=255)
                                                                                                                                     cAACATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCATTGa                  >  7:190040/1‑150 (MQ=255)
                                                                                                                                      aaCATGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGaa                 <  8:1155436/150‑1 (MQ=255)
                                                                                                                                       aCATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATc               <  4:1570702/151‑1 (MQ=255)
                                                                                                                                         aTGTAACTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTa             <  3:1102585/151‑1 (MQ=255)
                                                                                                                                             aaCTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCt          >  5:297309/1‑150 (MQ=255)
                                                                                                                                             aaCTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTc         >  3:838664/1‑151 (MQ=255)
                                                                                                                                               cTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCAt       >  2:1469737/1‑151 (MQ=255)
                                                                                                                                               cTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCAt       >  5:810466/1‑151 (MQ=255)
                                                                                                                                               cTTTGGGAAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCAt       >  3:1560048/1‑151 (MQ=255)
                                                                                                                                                 ttGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTTTACATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACACATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTg     >  3:371720/1‑151 (MQ=255)
                                                                                                                                                    ggCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGc  >  1:901638/1‑151 (MQ=255)
                                                                                                                                                     gCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCACAATGAATCTACCTCATTGGGc  >  2:1129778/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CGATATAATATATAAACACTATAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGAAGAATACTTTCCGAATGAAAGTTAAAGGTGTTTTATTTATAGATGGGGAGAGTCTTCGATAGTAGTGAGGTTGACAGATATCCGCTGCCTGATGAGCAATTATGCCCAATGAATCTACCTCATTGGGC  >  NC_000913/2456013‑2456311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑