Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,456,9930CA47.0% 244.8 / inf 483C403* (TGC→TGA) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base C (146/110);  new base A (129/98);  total (275/208)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-03

Only 100 of 488 total aligned reads displayed.

CATTATTGCTGTGCTGGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCG  >  NC_000913/4456843‑4457143
                                                                                                                                                      |                                                                                                                                                      
    attGCTGTGCTGGAGCCGCGCTCGAATCCCATGAAAATGGGGATCTGCAAAGAGGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGtt                                                                                                                                                     <  4:614886/150‑1 (MQ=255)
     ttGCTGTGCTGGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTc                                                                                                                                                    >  8:371/1‑150 (MQ=255)
        ctgtgctgGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGcc                                                                                                                                                >  2:956165/1‑151 (MQ=255)
          gtgctgGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTg                                                                                                                                              <  4:1453746/151‑1 (MQ=255)
          gtgctgGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTg                                                                                                                                              >  7:290619/1‑151 (MQ=255)
           tgctgGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAACTGGCAGAAGCCAGAGTTCAGCCTGc                                                                                                                                             >  7:510024/1‑151 (MQ=255)
            gctgGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGca                                                                                                                                            <  7:436220/151‑1 (MQ=255)
            gctgGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGc                                                                                                                                             >  5:269962/1‑150 (MQ=255)
                       gcTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTgg                                                                                                                                 >  4:331573/1‑151 (MQ=255)
                       gcTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTgg                                                                                                                                 >  3:1139542/1‑151 (MQ=255)
                        cTCGAATACCATGAAAATGGGGATCTGCAAATACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGCCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGc                                                                                                                                >  2:1082229/1‑151 (MQ=255)
                         tCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCg                                                                                                                               >  7:551587/1‑151 (MQ=255)
                         gcGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCg                                                                                                                               <  6:1023407/150‑1 (MQ=255)
                          cGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCg                                                                                                                               >  5:893282/1‑150 (MQ=255)
                          cGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTCGcc                                                                                                                               >  7:1177934/1‑149 (MQ=255)
                            aaTACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGAtg                                                                                                                            <  7:1028355/151‑1 (MQ=255)
                             aTACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTCGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGAtgt                                                                                                                           >  4:732410/1‑151 (MQ=255)
                               aCCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGAtgtg                                                                                                                          <  5:562971/150‑1 (MQ=255)
                                 cATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCTCACTGGAGTGGCGATGTGGAt                                                                                                                       >  3:750183/1‑151 (MQ=255)
                                 cATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGAt                                                                                                                       >  1:1722782/1‑151 (MQ=255)
                                   tGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATAc                                                                                                                     >  6:214729/1‑151 (MQ=255)
                                     aaaaTGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTACGTGGCAGGTGGCAGCAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGc                                                                                                                   >  2:1220382/1‑151 (MQ=255)
                                      aaaTGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCACCCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGcg                                                                                                                  >  6:1072916/1‑150 (MQ=255)
                                        aTGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTgg                                                                                                                >  4:1374547/1‑151 (MQ=255)
                                        aTGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTgg                                                                                                                <  8:603305/151‑1 (MQ=255)
                                         tGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGc                                                                                                               >  3:909663/1‑151 (MQ=255)
                                         tGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGc                                                                                                               >  2:175176/1‑151 (MQ=255)
                                          ggggATCTGCAAAGACGATCTGGCTCCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCa                                                                                                              <  1:288263/151‑1 (MQ=255)
                                          ggggATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCa                                                                                                              <  6:1484740/151‑1 (MQ=255)
                                                 tGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATAtgg                                                                                                       >  8:1231865/1‑151 (MQ=255)
                                                  gCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATAtg                                                                                                        <  3:165212/149‑1 (MQ=255)
                                                  gCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATAtggt                                                                                                      >  1:535250/1‑151 (MQ=255)
                                                  gCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATAtggt                                                                                                      <  2:581659/151‑1 (MQ=255)
                                                   cAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATAtggtg                                                                                                     >  3:1511695/1‑151 (MQ=255)
                                                    aaaGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATAtggtgg                                                                                                    >  5:1272782/1‑151 (MQ=255)
                                                      aGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGgtggtg                                                                                                  <  6:41392/151‑1 (MQ=255)
                                                        aCGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGa                                                                                                 >  1:1408362/1‑150 (MQ=255)
                                                          gATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGaaa                                                                                               >  8:1523101/1‑150 (MQ=255)
                                                           aTCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAAc                                                                                             >  3:854562/1‑151 (MQ=255)
                                                             cTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCg                                                                                           >  2:1451621/1‑151 (MQ=255)
                                                             cTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCg                                                                                           >  5:343973/1‑151 (MQ=255)
                                                              tGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGc                                                                                          >  1:101822/1‑151 (MQ=255)
                                                               ggCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGc                                                                                          <  8:925408/150‑1 (MQ=255)
                                                               ggCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCt                                                                                         <  4:1004358/151‑1 (MQ=255)
                                                                gCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTc                                                                                        <  7:1496801/151‑1 (MQ=255)
                                                                 cACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCa                                                                                       <  4:916737/151‑1 (MQ=255)
                                                                 cACCTTCATTAGGTCGTGCCGATGAAGTCTTCCAGCTGCAACCGGCGCATATTCCGTGGCAGGTGGAAGAAGTCGCACAAGCCTGCGTTCAGCCTGAACACTCGAGTGTCGATGTGGATAAGCTGGCAGATATGGTCGTGAAAAcagatc                                                                                        >  8:715460/1‑145 (MQ=255)
                                                                  aCCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTc                                                                                        >  6:143412/1‑149 (MQ=255)
                                                                      tCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCt                                                                                   <  1:1097002/150‑1 (MQ=255)
                                                                       cATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTgg                                                                                 <  1:49364/151‑1 (MQ=255)
                                                                          tAGGTCGTGCCGATGAAGTCTTACTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCg                                                                               >  6:518614/1‑150 (MQ=255)
                                                                               cGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGGGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCat                                                                          <  8:1748414/150‑1 (MQ=255)
                                                                                  gCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTc                                                                      <  5:209438/151‑1 (MQ=255)
                                                                                  gCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATCCGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTc                                                                      >  2:1291086/1‑151 (MQ=255)
                                                                                   ccGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCt                                                                     >  7:1226040/1‑151 (MQ=255)
                                                                                    cGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTg                                                                    <  7:189008/151‑1 (MQ=255)
                                                                                     gATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTgg                                                                   >  2:1537814/1‑151 (MQ=255)
                                                                                         aaGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGtgat                                                               <  1:983866/151‑1 (MQ=255)
                                                                                         aaGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGtgat                                                               >  8:1622128/1‑151 (MQ=255)
                                                                                         aaGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGtgat                                                               <  1:1518674/151‑1 (MQ=255)
                                                                                          aGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGtgatg                                                              <  5:1054051/151‑1 (MQ=255)
                                                                                           gTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTCGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGtgatg                                                              >  8:1051812/1‑150 (MQ=255)
                                                                                               tCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAg                                                            <  2:342524/148‑1 (MQ=255)
                                                                                               tCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAg                                                            <  8:25038/148‑1 (MQ=255)
                                                                                                      gcAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAAACGCTCAGCCTGGCGACCATATTCTGGTGTTGAGCAACGGCGGt                                                  <  5:1645977/151‑1 (MQ=255)
                                                                                                       cAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGtt                                                 >  6:911951/1‑151 (MQ=255)
                                                                                                       cAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGtt                                                 >  7:1291629/1‑151 (MQ=255)
                                                                                                       cAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGtt                                                 >  7:9367/1‑151 (MQ=255)
                                                                                                        aaCCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCTCACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGttt                                                <  5:747870/151‑1 (MQ=255)
                                                                                                        aaCCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGttt                                                <  5:1531197/151‑1 (MQ=255)
                                                                                                         aCCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGtttt                                               >  3:539223/1‑151 (MQ=255)
                                                                                                         aCCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGtttt                                               >  5:1380911/1‑151 (MQ=255)
                                                                                                          ccGGCGCATATTCCGTGGCTGGGGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACTCTGGAGTGGCGATGGGGATACGCGCGCAGATATGCTGGTGACAACCGCTCAGGCTGGCGACCATATTCTGGTGATGAGCAAGGCCGGtttt                                               <  3:313769/150‑1 (MQ=255)
                                                                                                          ccGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTtg                                              >  1:1319879/1‑151 (MQ=255)
                                                                                                           cGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTG‑AAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTtggt                                            <  3:187559/151‑1 (MQ=255)
                                                                                                           cGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTtgg                                             <  1:1270092/151‑1 (MQ=255)
                                                                                                            ggCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTtggt                                            <  3:1018385/151‑1 (MQ=255)
                                                                                                            ggCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTtggt                                            >  6:1149243/1‑151 (MQ=255)
                                                                                                             gcgcATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTtggtg                                           >  7:364945/1‑151 (MQ=255)
                                                                                                               gcATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTggg                                         >  7:1368483/1‑151 (MQ=255)
                                                                                                                cATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTggg                                         >  6:1684211/1‑150 (MQ=255)
                                                                                                                cATATTCCGTGGCAGGTGGAAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGTCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGa                                        <  3:612898/151‑1 (MQ=255)
                                                                                                                 atatTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGAt                                       >  8:1422531/1‑151 (MQ=255)
                                                                                                                   atTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATcc                                     <  2:789839/151‑1 (MQ=255)
                                                                                                                   atTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATcc                                     >  6:35365/1‑151 (MQ=255)
                                                                                                                      ccGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATc                                  <  2:439627/151‑1 (MQ=255)
                                                                                                                        gtggcaggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATc                                  >  1:8049/1‑149 (MQ=255)
                                                                                                                          ggcagggggcagAAGTGGCAGAAGCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGaa                              <  1:1218038/151‑1 (MQ=255)
                                                                                                                                     taagtggcacaagCCTGCGTTCAGCCTGCACACACGAGTGGCGATCTGCAAACGCTGGCAGATATGGTGCTGAAAAACCCACAGCCTGGAGACCATATTCAGCTGATGAGCAACGGCGCTTTAGGTCGGATCCATCACAAACTGATCGATg                   >  6:1291844/2‑151 (MQ=255)
                                                                                                                                      aagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGATTTGGTGGGATCCATCAGAAACTGCTGGATgg                  >  7:656773/1‑151 (MQ=255)
                                                                                                                                      aagtggcagaagCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGCCCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATgg                  >  8:843468/1‑151 (MQ=255)
                                                                                                                                      aagtggcagaagCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATgg                  <  8:505063/151‑1 (MQ=255)
                                                                                                                                        gtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGc                <  4:1071114/151‑1 (MQ=255)
                                                                                                                                          ggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGGGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCt               <  4:1670861/150‑1 (MQ=255)
                                                                                                                                           gcagaagCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTgg             >  4:218825/1‑151 (MQ=255)
                                                                                                                                            cagaagCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGc            <  1:695184/151‑1 (MQ=255)
                                                                                                                                             agaagCCTGAGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCg           <  3:1276982/151‑1 (MQ=255)
                                                                                                                                                  ccTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCgaaga       >  6:750242/1‑150 (MQ=255)
                                                                                                                                                     gCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGc   <  1:372874/151‑1 (MQ=255)
                                                                                                                                                      cGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCg  <  3:462162/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CATTATTGCTGTGCTGGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCG  >  NC_000913/4456843‑4457143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑