BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,068	0	A	G	55.1%	241.2 / inf	504	V45A (GTA→GCA)	yadV	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand): ref base A (111/114); new base G (134/142); total (245/259)
Fisher's exact test for biased strand distribution p-value = 9.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

Only 100 of 524 total aligned reads displayed.

TGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAG  >  NC_000913/155919‑156217

  aTTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGacgtgcgt                                                                                                                                                    >  8:1640423/1‑151 (MQ=255)

    tGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGtt                                                                                                                                                   <  2:903109/150‑1 (MQ=255)

    tGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTg                                                                                                                                                  >  7:1195218/1‑151 (MQ=255)

     gTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTg                                                                                                                                                  >  7:176391/1‑150 (MQ=255)

     gTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTg                                                                                                                                                  <  8:1594691/150‑1 (MQ=255)

     gTTTGCACACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGa                                                                                                                                                 <  1:838285/151‑1 (MQ=255)

       cggCCCACGTTTGGCATCTCAACGGGATTCTCGCGGCGTAGCTGTTAAAGGGCCTGTTATTCTGGCAGGCTGAGGGGTGTCATCGCCAGTATCTACCGAAGTCTGGCCCCGCACCGGGTTATTCCCTTTATTTTCCAGACGTTCGTTGac                                                                                                                                                <  6:1219160/148‑1 (MQ=255)

         gCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACt                                                                                                                                             >  5:1595168/1‑151 (MQ=255)

          cccACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACtt                                                                                                                                            <  4:1137050/151‑1 (MQ=255)

            cACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACtttt                                                                                                                                          >  3:412930/1‑151 (MQ=255)

             aCGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACttttt                                                                                                                                         >  5:779933/1‑151 (MQ=255)

             aCGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACttttt                                                                                                                                         <  8:1930377/151‑1 (MQ=255)

             aCGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACttttt                                                                                                                                         <  5:1821869/151‑1 (MQ=255)

             aCGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACttttt                                                                                                                                         >  3:768214/1‑151 (MQ=255)

             aCGTTTCGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCACCTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACttttt                                                                                                                                         <  8:26169/151‑1 (MQ=255)

              cGTTTGGCATCAATACGCGATAGTGGCGGGGTTGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACtttttt                                                                                                                                        <  1:1022702/151‑1 (MQ=255)

              cGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACtttttt                                                                                                                                        <  1:968996/151‑1 (MQ=255)

                tttGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCACCGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTg                                                                                                                                       >  6:731961/1‑150 (MQ=255)

                 ttGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGa                                                                                                                                      <  8:386690/150‑1 (MQ=255)

                 ttGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGa                                                                                                                                      <  8:549630/150‑1 (MQ=255)

                   tgCATCAATACGCGATACTGTCGGCGTAGAAGTAAAAGGGACTGTAATACTCCCAGGCTCAGCTTTGTCATCGCCAGTATCTAACCAACTCTGGACAATCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATc                                                                                                                                    <  5:502596/149‑1 (MQ=255)

                     cATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCACCTCTGGTCAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCt                                                                                                                                 >  4:776909/1‑151 (MQ=255)

                     cATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCt                                                                                                                                 >  5:1146116/1‑151 (MQ=255)

                     cATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCt                                                                                                                                 >  7:1557584/1‑151 (MQ=255)

                      aTCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCtt                                                                                                                                <  4:406134/151‑1 (MQ=255)

                        cAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCtttt                                                                                                                              >  6:1549773/1‑151 (MQ=255)

                          aTACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGGCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTtat                                                                                                                            <  4:266152/151‑1 (MQ=255)

                            aCGCGATACTGTCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTtata                                                                                                                           <  7:451708/150‑1 (MQ=255)

                            aCGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTtatat                                                                                                                          <  2:452330/151‑1 (MQ=255)

                            aCGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTtata                                                                                                                           <  4:844437/150‑1 (MQ=255)

                               cgATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATAtt                                                                                                                       <  3:1304910/151‑1 (MQ=255)

                                gATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTa                                                                                                                      <  6:1011897/151‑1 (MQ=255)

                                gATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATAtt                                                                                                                       >  7:81975/1‑150 (MQ=255)

                                 aTACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTAc                                                                                                                     >  6:465977/1‑151 (MQ=255)

                                 aTACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTAAATATTAc                                                                                                                     <  4:1395035/151‑1 (MQ=255)

                                   aCTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGCCGTACGTTGACACTTTTTTGATCGCTTTTATATATTAcgc                                                                                                                   >  7:1851771/1‑151 (MQ=255)

                                            gTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCg                                                                                                          >  8:1864001/1‑151 (MQ=255)

                                            gTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCg                                                                                                          >  4:473771/1‑151 (MQ=255)

                                              aGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGa                                                                                                         >  5:1300771/1‑150 (MQ=255)

                                              aGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGa                                                                                                         >  2:1114848/1‑150 (MQ=255)

                                                     aaaGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACa                                                                                                  >  8:1767927/1‑150 (MQ=255)

                                                         ggACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTc                                                                                             <  8:887888/151‑1 (MQ=255)

                                                         ggACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTc                                                                                             >  7:1299420/1‑151 (MQ=255)

                                                              gTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCa                                                                                         <  4:686414/150‑1 (MQ=255)

                                                              gTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCa                                                                                         <  6:563454/150‑1 (MQ=255)

                                                               tAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAAt                                                                                       >  1:1996584/1‑151 (MQ=255)

                                                                 aTACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAg                                                                                     <  8:1284791/151‑1 (MQ=255)

                                                                   aCTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGa                                                                                    <  5:262655/150‑1 (MQ=255)

                                                                   aCTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGAc                                                                                   >  3:1171989/1‑151 (MQ=255)

                                                                    cTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACg                                                                                  >  6:1444214/1‑151 (MQ=255)

                                                                      gCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACg                                                                                  <  4:1460927/149‑1 (MQ=255)

                                                                      gCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGAt                                                                                >  1:679044/1‑151 (MQ=255)

                                                                      gCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACg                                                                                  <  7:973117/149‑1 (MQ=255)

                                                                        cAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGa                                                                              <  7:207193/151‑1 (MQ=255)

                                                                             tCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTa                                                                          >  5:1991090/1‑150 (MQ=255)

                                                                             gcAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTaa                                                                         <  8:837393/150‑1 (MQ=255)

                                                                              cAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTa                                                                          <  6:905320/149‑1 (MQ=255)

                                                                              cAGCGTTGTCATCGACAGTATCTAACCAACTCTTGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGTGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTaaa                                                                        <  3:661332/151‑1 (MQ=255)

                                                                              cAGCGTTGTCATCGACAGTATCTAACCAACTCTGGACAAGCATCTGGTTATTCCCTTTATTTTCCAGACCGACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATATACGATGAACTaa                                                                         <  3:1660488/150‑1 (MQ=255)

                                                                                     gTCATCGGCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCAt                                                                  <  4:195751/150‑1 (MQ=255)

                                                                                     gTCATCGCCAGTATCTAACGAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCAt                                                                  <  1:305612/150‑1 (MQ=255)

                                                                                      tCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATAc                                                                >  5:1059463/1‑151 (MQ=255)

                                                                                      tCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATAc                                                                >  1:162568/1‑151 (MQ=255)

                                                                                      tCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATAc                                                                >  4:1651739/1‑151 (MQ=255)

                                                                                            ccAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGtt                                                           >  2:611774/1‑150 (MQ=255)

                                                                                             cAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTAc                                                         >  7:661751/1‑151 (MQ=255)

                                                                                             cAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTAc                                                         >  8:32038/1‑151 (MQ=255)

                                                                                             cAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTAc                                                         >  2:1663824/1‑151 (MQ=255)

                                                                                              aGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACg                                                        <  6:2006665/151‑1 (MQ=255)

                                                                                              aGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTCTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTCACg                                                        >  1:1825377/1‑151 (MQ=255)

                                                                                                    tAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCAt                                                  >  5:730383/1‑151 (MQ=255)

                                                                                                       ccAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATaaa                                               <  1:1208270/151‑1 (MQ=255)

                                                                                                         aaCTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGc                                             <  6:778355/151‑1 (MQ=255)

                                                                                                         aaCTCAGGACAACCAAAGGGTTATTACATTTATTTTCCAGACGTACGTTGACACTATTTTCATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGc                                             >  6:1670240/1‑151 (MQ=255)

                                                                                                           ctctGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtg                                           >  1:209310/1‑151 (MQ=255)

                                                                                                            actGGACAAGCAACGGGCTATTCCCTTTATTTTCCCGCCGTACGTTGACACTTTTTTGATCCCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgt                                          <  6:1867218/150‑1 (MQ=255)

                                                                                                              tGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgttg                                        >  3:576901/1‑151 (MQ=255)

                                                                                                               ggACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgttg                                        <  8:1161428/150‑1 (MQ=255)

                                                                                                               ggACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCtgt                                          <  2:337540/148‑1 (MQ=255)

                                                                                                                  cAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATg                                    <  7:1094817/151‑1 (MQ=255)

                                                                                                                      cAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTg                                >  6:6964/1‑151 (MQ=255)

                                                                                                                       aaCGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTgg                               >  3:930479/1‑151 (MQ=255)

                                                                                                                               aTTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaaa                        <  1:1278263/150‑1 (MQ=255)

                                                                                                                               aTTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGATGTTGTATGTTTGGTGTTaaa                        >  7:1043321/1‑150 (MQ=255)

                                                                                                                               aTTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaaaa                       <  2:768457/151‑1 (MQ=255)

                                                                                                                               aTTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaaaa                       <  2:1939634/151‑1 (MQ=255)

                                                                                                                                  cccTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTaaaaaa                     >  1:471154/1‑150 (MQ=255)

                                                                                                                                  cccTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAAc                    >  3:1939088/1‑151 (MQ=255)

                                                                                                                                   ccTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACa                   <  5:957942/151‑1 (MQ=255)

                                                                                                                                     tttATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAAAAt                  >  4:615917/1‑150 (MQ=255)

                                                                                                                                       tATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATaa                <  3:1486490/150‑1 (MQ=255)

                                                                                                                                           ttCCAGTCGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCAt           >  8:673490/1‑151 (MQ=255)

                                                                                                                                           ttCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCa            <  1:1416356/150‑1 (MQ=255)

                                                                                                                                             ccAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATc          <  4:1775960/150‑1 (MQ=255)

                                                                                                                                               aGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCt       >  8:1335708/1‑151 (MQ=255)

                                                                                                                                               aGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCt       >  2:1444871/1‑151 (MQ=255)

                                                                                                                                                 acgtgcgtTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTg      >  7:58506/1‑150 (MQ=255)

                                                                                                                                                 acgtacgtTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGa     <  5:328518/151‑1 (MQ=255)

                                                                                                                                                  cgtacgtTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGAt    >  2:1325330/1‑151 (MQ=255)

                                                                                                                                                     acgtTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGAt    >  7:1476324/1‑148 (MQ=255)

TGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAG  >  NC_000913/155919‑156217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`