Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,179,3830GA54.5% 114.9 / inf 493K423K (AAG→AAA) tolCtransport channel
Reads supporting (aligned to +/- strand):  ref base G (116/108);  new base A (133/135);  total (250/243)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 498 total aligned reads displayed.

CAAAGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCA  >  NC_000913/3179234‑3179533
                                                                                                                                                     |                                                                                                                                                      
cAAAGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAg                                                                                                                                                        <  1:1259679/150‑1 (MQ=255)
  aaGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCa                                                                                                                                                     <  4:194299/151‑1 (MQ=255)
   aGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTc                                                                                                                                                      >  7:1430285/1‑149 (MQ=255)
   aGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATc                                                                                                                                                      >  6:1473038/1‑149 (MQ=255)
   aGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAg                                                                                                                                                    <  8:481460/151‑1 (MQ=255)
   aGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAg                                                                                                                                                    <  1:1384881/151‑1 (MQ=255)
      tCATTAGACGCTTTGGAAGCTGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGct                                                                                                                                                  <  7:1602114/150‑1 (MQ=255)
       cATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCCAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGctct                                                                                                                                                <  2:1530243/151‑1 (MQ=255)
           ananNCGATGGANGNGGGNTANTCGNTNGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTggg                                                                                                                                             <  4:786416/150‑1 (MQ=255)
           aGACGGGATGGTAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGGTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGt                                                                                                                                            <  1:810335/151‑1 (MQ=255)
           aGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTggg                                                                                                                                             <  6:346209/150‑1 (MQ=255)
           aGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGt                                                                                                                                            >  3:593551/1‑151 (MQ=255)
           aGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGt                                                                                                                                            >  1:619302/1‑151 (MQ=255)
            gACGCGATGGAAGCGGGGGACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCGAAGCAAGAGCGGGCGAATGCGCGGTATAACTACCTGATTAATCAGCTGAATATTAAATCCGCTCTGGGTa                                                                                                                                           <  2:1397638/151‑1 (MQ=255)
             aCGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTAc                                                                                                                                          >  4:1689512/1‑151 (MQ=255)
              cgcgATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACg                                                                                                                                         <  2:701259/151‑1 (MQ=255)
              cgcgATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATCCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACg                                                                                                                                         >  1:1882543/1‑151 (MQ=255)
                cgATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCCCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGATCTGGGTACGtt                                                                                                                                       >  6:820434/1‑151 (MQ=255)
                  aTGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTg                                                                                                                                      >  3:133875/1‑150 (MQ=255)
                    ggAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGaa                                                                                                                                    <  4:1352636/150‑1 (MQ=255)
                     gAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACg                                                                                                                                  <  2:746857/151‑1 (MQ=255)
                       aGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGac                                                                                                                                >  8:820930/1‑150 (MQ=255)
                               aCTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATct                                                                                                                        <  5:312949/151‑1 (MQ=255)
                                ggcggtcggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATctg                                                                                                                       >  6:1440516/3‑151 (MQ=255)
                                cTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATctg                                                                                                                       >  3:940115/1‑151 (MQ=255)
                                 tcggtcggTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATctgc                                                                                                                      >  2:894517/1‑151 (MQ=255)
                                  cggtcggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATctgct                                                                                                                     <  2:1025823/151‑1 (MQ=255)
                                   ggtcggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATctgctg                                                                                                                    <  1:375238/151‑1 (MQ=255)
                                     tcggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGc                                                                                                                  >  5:895967/1‑151 (MQ=255)
                                      cggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGc                                                                                                                  >  6:599963/1‑150 (MQ=255)
                                      cggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCa                                                                                                                 <  2:988940/151‑1 (MQ=255)
                                       ggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCAc                                                                                                                >  1:1578114/1‑151 (MQ=255)
                                       ggtACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCAc                                                                                                                >  8:1723734/1‑151 (MQ=255)
                                            gcgTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAAc                                                                                                           >  2:1203927/1‑151 (MQ=255)
                                             cgTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAAc                                                                                                           >  1:1102761/1‑150 (MQ=255)
                                               tACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAAt                                                                                                        >  6:1887426/1‑151 (MQ=255)
                                                aacATTGTTGATGTGTTGTATGATACCACCACGTTGTAGAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAAt                                                                                                        <  7:921262/148‑1 (MQ=255)
                                                aCCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAAt                                                                                                        <  4:752656/150‑1 (MQ=255)
                                                 ccATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATgc                                                                                                      >  3:1190831/1‑151 (MQ=255)
                                                 ccATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATgc                                                                                                      <  8:1444853/151‑1 (MQ=255)
                                                     tgttgATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCt                                                                                                   <  8:2024940/150‑1 (MQ=255)
                                                        tgATGTGTTGGATGCTACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTAGCTGATTAATGAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAg                                                                                                <  8:159047/150‑1 (MQ=255)
                                                        tgATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAg                                                                                                <  1:1042978/150‑1 (MQ=255)
                                                          aTGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCaa                                                                                             <  3:350778/151‑1 (MQ=255)
                                                              gtTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATGAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAAcc                                                                                          <  6:673699/150‑1 (MQ=255)
                                                              gtTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAAcc                                                                                          <  3:762356/150‑1 (MQ=255)
                                                                tGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGt                                                                                       >  6:1008800/1‑151 (MQ=255)
                                                                 ggATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGt                                                                                       <  8:1038019/150‑1 (MQ=255)
                                                                      cGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCAc                                                                                 <  2:1338161/151‑1 (MQ=255)
                                                                       gACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACt                                                                                >  8:1329272/1‑151 (MQ=255)
                                                                       gACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACt                                                                                >  4:179347/1‑151 (MQ=255)
                                                                       gACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACt                                                                                >  2:2017869/1‑151 (MQ=255)
                                                                         ccaccaCGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTa                                                                               >  1:1155125/1‑150 (MQ=255)
                                                                         ccaccaCGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTaa                                                                              <  8:184967/151‑1 (MQ=255)
                                                                          caccacGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAAt                                                                             >  4:477402/1‑151 (MQ=255)
                                                                            ccacGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCACCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATcc                                                                           <  5:1794552/151‑1 (MQ=255)
                                                                             cacGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCg                                                                          <  3:840355/151‑1 (MQ=255)
                                                                              acGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCgg                                                                         <  4:899332/151‑1 (MQ=255)
                                                                                gTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGaa                                                                       <  8:633780/151‑1 (MQ=255)
                                                                                   gTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAAc                                                                    <  7:521500/151‑1 (MQ=255)
                                                                                    tACAACGCCAAGCAAGATCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAAc                                                                    <  6:942955/150‑1 (MQ=255)
                                                                                     agaaagCCAAGCAAGAGCTGGCGAATGCGGGTTATAACTACCTGATTAATCTGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGAACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAATCGt                                                                  <  3:1747425/146‑1 (MQ=255)
                                                                                     aCAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACg                                                                   <  7:1514634/150‑1 (MQ=255)
                                                                                      cAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGtt                                                                 <  3:2023744/151‑1 (MQ=255)
                                                                                       aaCGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTg                                                                >  6:999832/1‑151 (MQ=255)
                                                                                        aCGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGc                                                               <  7:123984/151‑1 (MQ=255)
                                                                                           ccAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCAcc                                                            >  2:303167/1‑151 (MQ=255)
                                                                                            caagcaagAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCg                                                           >  5:1121081/1‑151 (MQ=255)
                                                                                              agcaagAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGc                                                          >  7:774618/1‑150 (MQ=255)
                                                                                                caagAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCaaa                                                       <  5:1698556/151‑1 (MQ=255)
                                                                                                  agagCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACg                                                     >  4:325526/1‑151 (MQ=255)
                                                                                                   gagCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGc                                                    <  4:1256277/151‑1 (MQ=255)
                                                                                                     gCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCg                                                  <  8:1200801/151‑1 (MQ=255)
                                                                                                      gttgCGAATGCTCGTTATTACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCg                                                  <  3:1903153/147‑1 (MQ=255)
                                                                                                         gCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAAc                                              <  2:580471/151‑1 (MQ=255)
                                                                                                          cGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAAc                                              >  4:1524864/1‑150 (MQ=255)
                                                                                                           gAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAAc                                              >  6:1540391/1‑149 (MQ=255)
                                                                                                           gAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAg                                            <  2:1645750/151‑1 (MQ=255)
                                                                                                               gcgcGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAAt                                         <  2:1930228/150‑1 (MQ=255)
                                                                                                               gcgcGTTATAACTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATg                                        >  5:1270769/1‑151 (MQ=255)
                                                                                                                   gTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCACACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTAt                                    >  6:1709160/1‑151 (MQ=255)
                                                                                                                   gTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTAt                                    <  5:757396/151‑1 (MQ=255)
                                                                                                                         aCTACCTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGa                              <  5:200062/151‑1 (MQ=255)
                                                                                                                             ccTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGt                          >  2:482535/1‑151 (MQ=255)
                                                                                                                             ccTGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGt                          >  5:550799/1‑151 (MQ=255)
                                                                                                                               tGATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGtt                         >  3:1561461/1‑150 (MQ=255)
                                                                                                                                gATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGtt                         <  8:236354/149‑1 (MQ=255)
                                                                                                                                gATTAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTAt                       >  5:1390415/1‑151 (MQ=255)
                                                                                                                                   tAATCAGCTGAATATTAACTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCGGGAACAGAATGCTATTGCTGATGGTTATgcc                    >  5:1569782/1‑150 (MQ=255)
                                                                                                                                   tAATCAGCTGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCCATTGCTGATGGTTATgc                     <  3:541608/150‑1 (MQ=255)
                                                                                                                                     aTCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGcc                  >  6:1113206/1‑151 (MQ=255)
                                                                                                                                      tCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCAATTGCTGATGGTTATGCGCCt                 >  6:1289057/1‑151 (MQ=255)
                                                                                                                                        aGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTg                >  1:930020/1‑150 (MQ=255)
                                                                                                                                           tGAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGAt              >  5:1520646/1‑149 (MQ=255)
                                                                                                                                            gAATATTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATa             <  5:419722/149‑1 (MQ=255)
                                                                                                                                             aaTATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATACGGAAAACGTTGCACCGCAAACGCCGGAACAGAATCCTATTGCTGATGGTTAAGCTCATGATAGcc          >  4:1142213/1‑151 (MQ=255)
                                                                                                                                              atatTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGccc         >  3:1050806/1‑151 (MQ=255)
                                                                                                                                               tatTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGG‑TTCCACTAATCCGGAAAACGTTGCCCCGCAAACCCCGGAACAGAATGGTATTTCTTATGGTTTTTCGCCGGCTAGCCCgg       >  7:298768/1‑151 (MQ=255)
                                                                                                                                                atTAAATCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCg        <  2:39299/150‑1 (MQ=255)
                                                                                                                                                 ttAAATCAGGTCTGCGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTTAGCAAACCGGTTTACACTAATCCGGAAAACGTTTCAACGCAAACGCCGGAAAACAATGCTATTGCTAATGGTTATGATCCTGATAGACAGGc      >  7:1869743/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAAAGCTCATTAGACGCGATGGAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACGTTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTGAATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCGCTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAATGCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCA  >  NC_000913/3179234‑3179533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑