Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,589,9560GA54.7% 131.4 / inf 576L24L (CTC→CTT) ugpEsn‑glycerol‑3‑phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (125/135);  new base A (141/173);  total (267/309)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 583 total aligned reads displayed.

AGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACT  >  NC_000913/3589808‑3590106
                                                                                                                                                    |                                                                                                                                                      
  aaCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAgg                                                                                                                                                     >  1:1441367/1‑150 (MQ=255)
  aaCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGa                                                                                                                                                    <  2:523/151‑1 (MQ=255)
   aCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAggct                                                                                                                                                   >  2:915534/1‑149 (MQ=255)
   aCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGAt                                                                                                                                                   <  7:1901252/151‑1 (MQ=255)
    cGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATc                                                                                                                                                  >  8:337505/1‑151 (MQ=255)
     ggCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATc                                                                                                                                                  >  3:1345621/1‑150 (MQ=255)
     ggCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCa                                                                                                                                                 <  8:1803586/151‑1 (MQ=255)
      gcgcgcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCAc                                                                                                                                                >  4:21856/1‑151 (MQ=255)
         cgcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACcgc                                                                                                                                             >  6:1603320/1‑151 (MQ=255)
         cgcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACcgc                                                                                                                                             >  1:852194/1‑151 (MQ=255)
          gcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACcgcg                                                                                                                                            >  8:1714389/1‑151 (MQ=255)
          gcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACcgcg                                                                                                                                            >  5:1802909/1‑151 (MQ=255)
          gcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACcgcg                                                                                                                                            >  8:490972/1‑151 (MQ=255)
            tATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGAt                                                                                                                                          >  6:1409734/1‑151 (MQ=255)
             aTTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATc                                                                                                                                         <  4:197752/151‑1 (MQ=255)
               tCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATccc                                                                                                                                       >  4:1772384/1‑151 (MQ=255)
               tCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATcc                                                                                                                                        <  6:1932555/150‑1 (MQ=255)
                ggtgcgtaggccGTTCAGCGAGATGTTGTGGATGTTTTCGAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCg                                                                                                                                      <  3:751016/141‑1 (MQ=255)
                cGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATccc                                                                                                                                       <  1:1295687/150‑1 (MQ=255)
                cGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCg                                                                                                                                      >  8:1530396/1‑151 (MQ=255)
                cGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCg                                                                                                                                      >  4:671688/1‑151 (MQ=255)
                cGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCg                                                                                                                                      >  2:13427/1‑151 (MQ=255)
                    ccTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGa                                                                                                                                  <  5:1195026/151‑1 (MQ=255)
                      tACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGCCGGCCTGTTTATCCAGCGTCGCCGCGCCAAACGCCACGTACAGCGGGAAGAGGATCCCCGCGATCCCGAGGATc                                                                                                                                >  3:1942339/1‑151 (MQ=255)
                           cGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCAt                                                                                                                           <  2:1733767/151‑1 (MQ=255)
                           cGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCAt                                                                                                                           >  4:559720/1‑151 (MQ=255)
                            gTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATg                                                                                                                          <  6:1118038/151‑1 (MQ=255)
                               cACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGt                                                                                                                        <  4:328509/150‑1 (MQ=255)
                               cACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGt                                                                                                                        >  3:864060/1‑150 (MQ=255)
                               cACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGt                                                                                                                        <  5:748104/150‑1 (MQ=255)
                                aCCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTAt                                                                                                                      >  4:1633820/1‑151 (MQ=255)
                                 cccAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATg                                                                                                                     <  6:921774/151‑1 (MQ=255)
                                  ccAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATgg                                                                                                                    >  2:392459/1‑151 (MQ=255)
                                   cAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGc                                                                                                                   >  1:308994/1‑151 (MQ=255)
                                    aGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCt                                                                                                                  <  1:760109/151‑1 (MQ=255)
                                     gATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTg                                                                                                                 <  7:944386/151‑1 (MQ=255)
                                        gttgtGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGaa                                                                                                               <  5:1626719/150‑1 (MQ=255)
                                        gttgtGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGaa                                                                                                               <  6:183252/150‑1 (MQ=255)
                                           gtgGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATc                                                                                                           <  7:1496476/151‑1 (MQ=255)
                                            tgGATTTTTTCCAGGAGATGTGTGGGTGGGTTGAGCGTCATTGGTGGGGCATAGACTGCCTGTTTATCCAGCGTCGCCGCGTCAAACGCCACGTACAGCTGGAAGAGGATCACCGCTATCCCGATGATCAGCATGGTATGTGTGAATATc                                                                                                           <  8:1952872/150‑1 (MQ=255)
                                             ggATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGt                                                                                                         <  5:445793/151‑1 (MQ=255)
                                             ggATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGGCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCg                                                                                                          <  4:759995/150‑1 (MQ=255)
                                             ggATGGTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGt                                                                                                         <  6:1210565/151‑1 (MQ=255)
                                              gATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTc                                                                                                        >  4:969028/1‑151 (MQ=255)
                                                 gTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAg                                                                                                      <  6:78863/150‑1 (MQ=255)
                                                 gTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGc                                                                                                     >  1:597710/1‑151 (MQ=255)
                                                 gTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGc                                                                                                     >  8:768164/1‑151 (MQ=255)
                                                  ttttCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGCCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGAGGGAAGAGGATCACCGCGATCCCGAGCAACAGCATGGTATCGCATAATATCGACATcc                                                                                                    >  7:293641/1‑151 (MQ=255)
                                                   tttCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGcc                                                                                                    <  7:484817/150‑1 (MQ=255)
                                                       cagcagATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacgga                                                                                               <  3:1645282/151‑1 (MQ=255)
                                                        agcagATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggac                                                                                              <  1:461364/151‑1 (MQ=255)
                                                        agcagATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggac                                                                                              <  4:1028727/151‑1 (MQ=255)
                                                            gATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGtt                                                                                          <  7:1080108/151‑1 (MQ=255)
                                                                 gtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCa                                                                                      <  5:1366797/150‑1 (MQ=255)
                                                                   gCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAAt                                                                                    <  2:1152286/150‑1 (MQ=255)
                                                                               cGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGc                                                                        >  2:208483/1‑150 (MQ=255)
                                                                                gTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCAc                                                                      >  3:1476627/1‑151 (MQ=255)
                                                                                 tCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCAcc                                                                     >  8:1303618/1‑151 (MQ=255)
                                                                                  cATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCt                                                                    <  8:612499/151‑1 (MQ=255)
                                                                                      ggCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCt                                                                <  6:316344/151‑1 (MQ=255)
                                                                                        cgcgGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCttt                                                              <  1:1735340/151‑1 (MQ=255)
                                                                                        cgcgGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGGATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCttt                                                              >  7:814150/1‑151 (MQ=255)
                                                                                         gcgGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGTGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTc                                                             <  5:30523/151‑1 (MQ=255)
                                                                                             cATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAAc                                                          >  5:677923/1‑150 (MQ=255)
                                                                                                aGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATa                                                       <  1:1807440/150‑1 (MQ=255)
                                                                                                aGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAg                                                      <  8:851438/151‑1 (MQ=255)
                                                                                                 gACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACTGGGGTAAGAGGATCACCGCGATCCCGAGGATCATCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGc                                                     <  1:1009007/151‑1 (MQ=255)
                                                                                                 gACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGc                                                     <  4:1346979/151‑1 (MQ=255)
                                                                                                  aCGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCg                                                    >  5:959640/1‑151 (MQ=255)
                                                                                                    ggCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGa                                                  <  1:1244625/151‑1 (MQ=255)
                                                                                                     gCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGaa                                                 <  8:1108098/151‑1 (MQ=255)
                                                                                                     gCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGa                                                  >  3:319444/1‑150 (MQ=255)
                                                                                                       gtGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACt                                               <  4:1100045/150‑1 (MQ=255)
                                                                                                       cTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACt                                               >  7:134856/1‑151 (MQ=255)
                                                                                                         gTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTg                                              <  2:740138/150‑1 (MQ=255)
                                                                                                            tATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGcacc                                          >  3:589672/1‑151 (MQ=255)
                                                                                                             aTCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGcacca                                         <  7:1180609/151‑1 (MQ=255)
                                                                                                                cAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCg                                      <  3:478505/151‑1 (MQ=255)
                                                                                                                cAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCg                                      <  8:162356/151‑1 (MQ=255)
                                                                                                                 aGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGt                                     <  5:245758/151‑1 (MQ=255)
                                                                                                                  gCGTCGCCGAGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCGGAGCATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACGACCGTc                                    <  8:1414342/151‑1 (MQ=255)
                                                                                                                     tCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGc                                 >  6:1730829/1‑151 (MQ=255)
                                                                                                                        ccgcGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACg                              <  1:167877/151‑1 (MQ=255)
                                                                                                                        cagagACAAACGCCAAGTACAGCGGGAAAAGGATCACCTCGATCCCGAGGATCATCATGGTATGTCTGAATATCGTCAGCCACGGACGGTTGTCAATCATTGGTAACGGACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACg                              <  6:604697/147‑1 (MQ=255)
                                                                                                                           cgACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATg                           >  3:2009239/1‑151 (MQ=255)
                                                                                                                            gACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATg                           >  6:485922/1‑150 (MQ=255)
                                                                                                                            gACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATg                           >  7:382541/1‑150 (MQ=255)
                                                                                                                                aaCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAg                      <  5:1518439/151‑1 (MQ=255)
                                                                                                                                 aCGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAgg                     <  7:1679841/151‑1 (MQ=255)
                                                                                                                                 aCGCCACGTACAGCGGGAAGAGGAGCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAgg                     <  3:1158088/151‑1 (MQ=255)
                                                                                                                                 aCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAgg                     <  2:963435/151‑1 (MQ=255)
                                                                                                                                  cGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGa                    <  2:1814272/151‑1 (MQ=255)
                                                                                                                                        gTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGCCGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATc               >  4:1509700/1‑150 (MQ=255)
                                                                                                                                        gTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCa              <  2:1477005/151‑1 (MQ=255)
                                                                                                                                          aCAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCaa             <  2:616830/150‑1 (MQ=255)
                                                                                                                                            aGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAacac          >  5:856823/1‑151 (MQ=255)
                                                                                                                                              cGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCAATGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAAcacc         >  7:1633930/1‑150 (MQ=255)
                                                                                                                                               gggAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAAcaccac       >  7:588748/1‑151 (MQ=255)
                                                                                                                                               gggAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAAcaccac       >  7:1012058/1‑151 (MQ=255)
                                                                                                                                                 gAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCg     >  1:1888246/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACT  >  NC_000913/3589808‑3590106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑