Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,486,8690TA56.4% 297.2 / inf 546L218M (TTG→ATG) lptFlipopolysaccharide export ABC permease
Reads supporting (aligned to +/- strand):  ref base T (108/129);  new base A (151/156);  total (260/286)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 552 total aligned reads displayed.

GATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAAC  >  NC_000913/4486719‑4487018
                                                                                                                                                      |                                                                                                                                                     
gATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAt                                                                                                                                                       <  7:250770/151‑1 (MQ=255)
   ttCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATg                                                                                                                                                     <  3:1055468/150‑1 (MQ=255)
    tCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGtt                                                                                                                                                   >  6:712861/1‑151 (MQ=255)
     cAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGtt                                                                                                                                                   <  7:1096552/150‑1 (MQ=255)
     cAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGtt                                                                                                                                                   <  6:761876/150‑1 (MQ=255)
     cAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGtt                                                                                                                                                   <  5:132425/150‑1 (MQ=255)
       aaGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACg                                                                                                                                                >  4:1909925/1‑151 (MQ=255)
        aGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGt                                                                                                                                               <  1:316964/151‑1 (MQ=255)
        aGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGt                                                                                                                                               >  3:213012/1‑151 (MQ=255)
         gATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTg                                                                                                                                              <  5:1906343/151‑1 (MQ=255)
              gtTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCAAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTc                                                                                                                                         >  4:142663/1‑151 (MQ=255)
                tCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTcc                                                                                                                                        <  1:1541897/150‑1 (MQ=255)
                 ccTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCg                                                                                                                                       <  2:1543014/150‑1 (MQ=255)
                  cTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGc                                                                                                                                      >  1:63011/1‑150 (MQ=255)
                   tCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCAt                                                                                                                                    >  2:1942207/1‑151 (MQ=255)
                   tCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCAt                                                                                                                                    >  1:1753777/1‑151 (MQ=255)
                   tCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCAt                                                                                                                                    >  6:336584/1‑151 (MQ=255)
                    cgcgCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCAt                                                                                                                                    <  3:720970/150‑1 (MQ=255)
                     gcgcAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTa                                                                                                                                  <  8:1761359/151‑1 (MQ=255)
                     gcgcAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTa                                                                                                                                  <  7:1210959/151‑1 (MQ=255)
                      cgcAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTAc                                                                                                                                 >  1:460126/1‑151 (MQ=255)
                        cAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg                                                                                                                               >  2:111227/1‑151 (MQ=255)
                         aaaTTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGa                                                                                                                              <  4:803895/151‑1 (MQ=255)
                         aaaTTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACgg                                                                                                                               >  6:1553574/1‑150 (MQ=255)
                          aaTTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGAc                                                                                                                             <  5:863407/151‑1 (MQ=255)
                           aTTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACt                                                                                                                            <  4:1231815/151‑1 (MQ=255)
                             tCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCCCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTCCGGACTTc                                                                                                                          >  6:1374822/1‑151 (MQ=255)
                             tCGACCAAAAGGTAATGCACCTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTc                                                                                                                          >  2:833938/1‑151 (MQ=255)
                              cGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTcc                                                                                                                         >  3:84195/1‑151 (MQ=255)
                               gACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTcc                                                                                                                         >  4:593894/1‑150 (MQ=255)
                                    aaaGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGAtt                                                                                                                   <  1:902566/151‑1 (MQ=255)
                                     aaGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTa                                                                                                                  <  8:1347454/151‑1 (MQ=255)
                                        gTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATc                                                                                                                >  4:333130/1‑150 (MQ=255)
                                          aaTGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAg                                                                                                              <  6:915723/150‑1 (MQ=255)
                                          aaTGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAg                                                                                                              <  4:13357/150‑1 (MQ=255)
                                             gCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCg                                                                                                           >  2:571629/1‑150 (MQ=255)
                                              cACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt                                                                                                         >  4:159541/1‑151 (MQ=255)
                                                   ccTTCTGTGGTGGTGTCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCAtt                                                                                                     <  6:1174372/150‑1 (MQ=255)
                                                   ccTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTg                                                                                                    >  1:1855609/1‑151 (MQ=255)
                                                    cTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTgg                                                                                                   >  7:654272/1‑151 (MQ=255)
                                                      tCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTgg                                                                                                   <  8:62896/149‑1 (MQ=255)
                                                      tCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGt                                                                                                  <  4:921359/150‑1 (MQ=255)
                                                        tgtgGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCAc                                                                                               >  2:256395/1‑151 (MQ=255)
                                                         gtgggggtgGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCAcc                                                                                              <  8:1926513/151‑1 (MQ=255)
                                                          tggtggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCAccc                                                                                             >  7:416147/1‑150 (MQ=255)
                                                            gtggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAgg                                                                                           <  1:1580127/151‑1 (MQ=255)
                                                            gtgggggCCGATTCCGGACATTTCACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGCCCGCGCTTAGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAgg                                                                                           <  6:1422975/151‑1 (MQ=255)
                                                                 ggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg                                                                                      <  2:463636/151‑1 (MQ=255)
                                                                   ccGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGgc                                                                                    >  5:1738373/1‑151 (MQ=255)
                                                                    cGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGgcg                                                                                   <  8:1633016/151‑1 (MQ=255)
                                                                    cGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGgcg                                                                                   >  2:349531/1‑151 (MQ=255)
                                                                    cGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGgcg                                                                                   >  4:1758644/1‑151 (MQ=255)
                                                                        tCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTc                                                                                <  3:358375/150‑1 (MQ=255)
                                                                          cGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCgcc                                                                             >  5:944243/1‑149 (MQ=255)
                                                                           ggACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGAcc                                                                            <  3:1217166/151‑1 (MQ=255)
                                                                            gACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGAccc                                                                           <  5:1507405/151‑1 (MQ=255)
                                                                              cATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCg                                                                          >  8:1093362/1‑150 (MQ=255)
                                                                                 ttAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAAc                                                                       <  5:1095949/150‑1 (MQ=255)
                                                                                   aaCCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGAt                                                                    >  2:1686825/1‑151 (MQ=255)
                                                                                    aCCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATa                                                                   <  2:1443359/151‑1 (MQ=255)
                                                                                     cccAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCACCCAGGGAACGCGCTTCGAAGGCACTGAAATGTTACGTGATTTCCGCATTACGGACTTCAAGGATTATCAGGCGATCATTGGTCACCATGCGGTGGCGCTCGACCCGAACGAtcc                                                                  >  8:684305/1‑149 (MQ=255)
                                                                                      ccAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATAcc                                                                 >  1:228041/1‑151 (MQ=255)
                                                                                         gCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGAc                                                              <  6:479756/151‑1 (MQ=255)
                                                                                            gcgcGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCa                                                            <  6:98242/150‑1 (MQ=255)
                                                                                            gcgcGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCa                                                            <  8:2043786/150‑1 (MQ=255)
                                                                                                    gCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACAt                                                   <  2:1010692/151‑1 (MQ=255)
                                                                                                      tCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATgc                                                 >  7:1359806/1‑151 (MQ=255)
                                                                                                       cctaGGTCGTCACTCTCAACCAGGGAACGCGCTGCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCTGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATgcg                                                <  3:1857107/148‑1 (MQ=255)
                                                                                                         cAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATgcgc                                               >  5:576134/1‑150 (MQ=255)
                                                                                                         cAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGca                                              <  8:564053/151‑1 (MQ=255)
                                                                                                            ggcgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACAt                                           <  6:1875268/149‑1 (MQ=255)
                                                                                                             tcgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACAtt                                          >  4:1011304/1‑151 (MQ=255)
                                                                                                               gtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATtgt                                        <  1:1094335/151‑1 (MQ=255)
                                                                                                                 cACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTgg                                      <  5:379732/151‑1 (MQ=255)
                                                                                                                     ctcAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAac                                   >  2:924156/1‑150 (MQ=255)
                                                                                                                       cAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAaca                                  <  6:1939323/149‑1 (MQ=255)
                                                                                                                       cAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACt                                <  8:342561/151‑1 (MQ=255)
                                                                                                                       cAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACt                                <  3:65199/151‑1 (MQ=255)
                                                                                                                           cAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGaca                            <  3:147029/151‑1 (MQ=255)
                                                                                                                             gggAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACAcc                          <  7:1999041/151‑1 (MQ=255)
                                                                                                                             gggAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACAcc                          >  7:1715303/1‑151 (MQ=255)
                                                                                                                                aaCGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCTTTACGGACTTCGAGGATTATCAGGCGATCATTGGTCACGAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGAt                       <  6:576529/151‑1 (MQ=255)
                                                                                                                                aaCGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGAt                       <  6:1713791/151‑1 (MQ=255)
                                                                                                                                 aCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATc                      >  7:15473/1‑151 (MQ=255)
                                                                                                                                 aCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATc                      <  2:682228/151‑1 (MQ=255)
                                                                                                                                 aCGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGTATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATc                      <  6:1089256/151‑1 (MQ=255)
                                                                                                                                 aCGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATc                      <  3:1252602/151‑1 (MQ=255)
                                                                                                                                    cgcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTg                   >  8:1190324/1‑151 (MQ=255)
                                                                                                                                     gcTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGc                  >  3:1547570/1‑151 (MQ=255)
                                                                                                                                      cTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCt                 >  1:1217434/1‑151 (MQ=255)
                                                                                                                                       ttCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTc                >  3:1114737/1‑151 (MQ=255)
                                                                                                                                        tCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTcc               >  8:834169/1‑150 (MQ=255)
                                                                                                                                        tCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTc                <  4:273172/150‑1 (MQ=255)
                                                                                                                                           aaGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCgcgc            >  1:769057/1‑151 (MQ=255)
                                                                                                                                               cACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAgaa        <  3:541173/151‑1 (MQ=255)
                                                                                                                                               cACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAga         >  5:1561740/1‑150 (MQ=255)
                                                                                                                                                  tGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGCCACCGATCGTGCTCTCGCAgaactg     >  7:1929754/1‑151 (MQ=255)
                                                                                                                                                   gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAgaactga    <  2:1350238/151‑1 (MQ=255)
                                                                                                                                                   gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAgaact      <  7:907097/149‑1 (MQ=255)
                                                                                                                                                    cATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAgaactga    >  2:196295/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAAC  >  NC_000913/4486719‑4487018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑