Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,585,6730CT54.7% 138.2 / inf 597W363* (TGG→TGA) hsdRendonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base C (117/153);  new base T (167/159);  total (285/312)
Fisher's exact test for biased strand distribution p-value = 5.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

Only 100 of 600 total aligned reads displayed.

CCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTC  >  NC_000913/4585523‑4585823
                                                                                                                                                      |                                                                                                                                                      
     cGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCt                                                                                                                                                   <  4:1253740/151‑1 (MQ=255)
     cGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCt                                                                                                                                                   <  1:928901/151‑1 (MQ=255)
           aGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTc                                                                                                                                             >  1:1424122/1‑151 (MQ=255)
              cTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCAt                                                                                                                                           >  6:796023/1‑150 (MQ=255)
                    aCATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTgg                                                                                                                                    >  1:450383/1‑151 (MQ=255)
                    aCATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTgg                                                                                                                                    >  4:895683/1‑151 (MQ=255)
                     cATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGGCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGc                                                                                                                                   <  1:728524/151‑1 (MQ=255)
                      atatTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGc                                                                                                                                   >  4:439284/1‑150 (MQ=255)
                            cGGGTTTCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTATGGCACTGCtt                                                                                                                            <  3:577726/151‑1 (MQ=255)
                             gggTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTc                                                                                                                           >  7:1542772/1‑151 (MQ=255)
                             gggTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTc                                                                                                                           >  5:1239438/1‑151 (MQ=255)
                                   cacgcacgTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCa                                                                                                                      <  5:1556778/150‑1 (MQ=255)
                                   cacgcacgTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCAc                                                                                                                     <  7:124107/151‑1 (MQ=255)
                                      gcacgTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTc                                                                                                                  >  4:1628443/1‑151 (MQ=255)
                                       cacgTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCa                                                                                                                 <  2:626059/151‑1 (MQ=255)
                                       cacgTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCa                                                                                                                 <  1:269489/151‑1 (MQ=255)
                                        acgTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCAt                                                                                                                <  5:753421/151‑1 (MQ=255)
                                        acgTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCAt                                                                                                                >  6:1588320/1‑151 (MQ=255)
                                          gTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATcc                                                                                                              <  5:1371359/151‑1 (MQ=255)
                                            cgcgATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCgg                                                                                                            >  6:1326838/1‑151 (MQ=255)
                                            cgcgATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCgg                                                                                                            >  3:1386500/1‑151 (MQ=255)
                                               gATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGGCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGa                                                                                                         <  3:197718/151‑1 (MQ=255)
                                                aTACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTg                                                                                                          >  6:653739/1‑149 (MQ=255)
                                                 tACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCAGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGagt                                                                                                       >  3:306122/1‑151 (MQ=255)
                                                  aCCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGagta                                                                                                      <  1:1829993/151‑1 (MQ=255)
                                                    cAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGagtagt                                                                                                    >  4:730745/1‑151 (MQ=255)
                                                        tGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTc                                                                                                >  6:884894/1‑151 (MQ=255)
                                                         gCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTca                                                                                               <  7:1962043/151‑1 (MQ=255)
                                                             cTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCCGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCTTCCGGTGAGTAGTGCTcaagc                                                                                            >  3:1203408/1‑150 (MQ=255)
                                                                ttGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAgg                                                                                        >  6:374410/1‑151 (MQ=255)
                                                                 tGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGt                                                                                       >  6:1052130/1‑151 (MQ=255)
                                                                  ggTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGt                                                                                       <  1:298466/150‑1 (MQ=255)
                                                                   gTCTTCATTGTTGCGCGGTATTCGCGCCCTTTTGTCGATTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTTGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGttt                                                                                     <  7:1795646/151‑1 (MQ=255)
                                                                   gTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTTGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGtt                                                                                      <  2:1336650/150‑1 (MQ=255)
                                                                     cTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTcc                                                                                   >  4:988513/1‑151 (MQ=255)
                                                                       tCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTccc                                                                                  <  6:241939/150‑1 (MQ=255)
                                                                       tCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTc                                                                                    <  1:618199/148‑1 (MQ=255)
                                                                       tCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCg                                                                                 >  7:1315201/1‑151 (MQ=255)
                                                                        cATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCg                                                                                 <  8:1069170/150‑1 (MQ=255)
                                                                        cATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCCGTGAGTAGTGCTCAAGCAAGGTTTCCCg                                                                                 <  5:530437/150‑1 (MQ=255)
                                                                        cATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCg                                                                                 <  3:157260/150‑1 (MQ=255)
                                                                        cATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCg                                                                                 <  8:300496/150‑1 (MQ=255)
                                                                            gttgCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGTGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGa                                                                            <  8:1895169/151‑1 (MQ=255)
                                                                            gttgCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGCGGATTTTAAACCGTTGTTAGCAGCTGGTCTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTAAAGCAAGGTATCCCGCAgg                                                                             >  7:297422/1‑150 (MQ=255)
                                                                              tgCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGaa                                                                           <  3:1496855/150‑1 (MQ=255)
                                                                               gcgcGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGc                                                                         <  3:1671522/151‑1 (MQ=255)
                                                                                 gcgGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGACAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGcc                                                                        >  2:1029891/1‑150 (MQ=255)
                                                                                 gcgGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTGTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCCGTGAGTAGTGCTCACGCAAGGTTTCCCGCAGGAAGcc                                                                        >  5:938938/1‑150 (MQ=255)
                                                                                   ggTATTCGCTCCCGTTGGTCGAGTAGCAGAAGGGGATTTTACCCCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCAtt                                                                     <  6:513896/151‑1 (MQ=255)
                                                                                   ggTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAG‑AGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTa                                                                    <  8:1217433/151‑1 (MQ=255)
                                                                                    gTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTa                                                                    <  4:1929404/151‑1 (MQ=255)
                                                                                    gTATTCGCGCCCGTTGGTCGAGTAGCAGAAGCGGATTTTAAACCGTTGTTTGCCGCTCGTGTCCTGCCACATGGTATCATACTCTGGCACTGCTTCATGCACTTCATCCAGTGAGTAGTGCTCAAGCAAGCTTTCCAGCACGAAGCCAtt                                                                     >  8:1615083/1‑150 (MQ=255)
                                                                                      aTTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTAt                                                                   <  4:1094235/150‑1 (MQ=255)
                                                                                      aTTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATc                                                                  >  2:1213440/1‑151 (MQ=255)
                                                                                      aTTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTAt                                                                   <  3:1796168/150‑1 (MQ=255)
                                                                                        tCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCGTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCg                                                                 <  3:477059/150‑1 (MQ=255)
                                                                                        tCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCg                                                                 >  2:1858700/1‑150 (MQ=255)
                                                                                         cgcgCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCCTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGaa                                                               <  7:1658785/151‑1 (MQ=255)
                                                                                          gcgcCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGaaa                                                              <  4:599915/151‑1 (MQ=255)
                                                                                               cGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACAttt                                                         <  5:1117560/151‑1 (MQ=255)
                                                                                                gTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTa                                                        <  8:85625/151‑1 (MQ=255)
                                                                                                   ggTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTg                                                     >  8:635317/1‑151 (MQ=255)
                                                                                                       gAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGc                                                 >  2:1656778/1‑151 (MQ=255)
                                                                                                       gAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGc                                                 >  2:954781/1‑151 (MQ=255)
                                                                                                       gAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGTTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGc                                                 <  1:1013203/151‑1 (MQ=255)
                                                                                                       gAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGc                                                 <  1:2025279/151‑1 (MQ=255)
                                                                                                        aGTAGCAGAAGGTGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCg                                                <  6:1665408/151‑1 (MQ=255)
                                                                                                        aGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCg                                                >  7:685165/1‑151 (MQ=255)
                                                                                                          tAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGAt                                              >  1:515557/1‑151 (MQ=255)
                                                                                                          tAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGAt                                              >  5:356838/1‑151 (MQ=255)
                                                                                                              aGAAGGGGATTTTACACCGGTGTTTGGCGTTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCTCTTCATCCGGTGATTAGTGCTCAAGCTAGTTTTCCCGCTGGAAGCCATTATCGAAACATTTACTGTAGCGATACgc                                          <  8:43939/151‑2 (MQ=255)
                                                                                                               gAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGAc                                         <  5:1775323/151‑1 (MQ=255)
                                                                                                                aaGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACt                                        >  7:980606/1‑151 (MQ=255)
                                                                                                                   gggATTTTAAACCGTTGTTTGCCGCTGGTGTCCTTCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCAt                                     <  3:1109598/151‑1 (MQ=255)
                                                                                                                   gggATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCAt                                     >  5:625597/1‑151 (MQ=255)
                                                                                                                   gggATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCAt                                     <  2:476558/151‑1 (MQ=255)
                                                                                                                    ggATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCAtt                                    >  4:1481102/1‑151 (MQ=255)
                                                                                                                    ggATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCAtt                                    <  6:1828679/151‑1 (MQ=255)
                                                                                                                     gATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTg                                   >  8:1314097/1‑151 (MQ=255)
                                                                                                                        tttAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAg                                 <  4:1350507/150‑1 (MQ=255)
                                                                                                                         ttAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGcc                               >  4:247408/1‑151 (MQ=255)
                                                                                                                           aaaCCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCt                              <  8:1501408/150‑1 (MQ=255)
                                                                                                                           aaaCCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCt                              <  1:1570051/150‑1 (MQ=255)
                                                                                                                           aaaCCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACAGCTTCATGCACTTCATCCGGTGAGTAGTTCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTg                             >  8:1764649/1‑151 (MQ=255)
                                                                                                                           aaaCCGGTGTTTGCCGCTGGTGCCCTGTCAGCGGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTCGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTg                             <  3:850728/151‑1 (MQ=255)
                                                                                                                            aagcGTTGTTTGTCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTgg                            <  7:14516/148‑1 (MQ=255)
                                                                                                                             aCCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGc                           <  8:2040512/151‑1 (MQ=255)
                                                                                                                                gttgttTGCCGCTGGTGTCCTGTCAGCAGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCggg                        <  2:306103/151‑1 (MQ=255)
                                                                                                                                 ttgttTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCggg                        >  7:859587/1‑150 (MQ=255)
                                                                                                                                  tgttTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGa                       <  6:1480983/150‑1 (MQ=255)
                                                                                                                                      tGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTc                  >  7:526883/1‑151 (MQ=255)
                                                                                                                                       gCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCg                 <  7:1023386/151‑1 (MQ=255)
                                                                                                                                       gCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTc                  <  1:1756265/150‑1 (MQ=255)
                                                                                                                                       gCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCg                 <  4:1733172/151‑1 (MQ=255)
                                                                                                                                       gCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACGGCTTCATGCACTTCATCCGGGGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCg                 <  2:1956091/151‑1 (MQ=255)
                                                                                                                                          gCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGat               >  5:1967519/1‑150 (MQ=255)
                                                                                                                                            tGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATAtt            >  1:80498/1‑151 (MQ=255)
                                                                                                                                             ggTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTg           <  1:1159308/151‑1 (MQ=255)
                                                                                                                                              gtgtCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGt          <  2:1420829/151‑1 (MQ=255)
                                                                                                                                                  ccTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACg      <  8:783299/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTC  >  NC_000913/4585523‑4585823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑