Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913358,4840GA56.1% 131.3 / inf 453S69S (AGC→AGTcynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (117/81);  new base A (138/115);  total (257/196)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-02

Only 100 of 456 total aligned reads displayed.

GCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACG  >  NC_000913/358334‑358633
                                                                                                                                                      |                                                                                                                                                     
gCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCgg                                                                                                                                                       <  5:122469/151‑1 (MQ=255)
  tGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGGTTCCCCGCCCCCAGTTCCTGTAACGCCCGGCt                                                                                                                                                     >  4:1085629/1‑151 (MQ=255)
   gggATAGCGCGCATAGAAATCCGCCATTAAGGAGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTg                                                                                                                                                    <  7:232431/151‑1 (MQ=255)
       tAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGt                                                                                                                                                >  5:2000750/1‑151 (MQ=255)
        aGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGt                                                                                                                                                >  2:1607461/1‑150 (MQ=255)
          cgcgcATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACt                                                                                                                                             <  4:258360/151‑1 (MQ=255)
           gcgcATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTg                                                                                                                                            <  5:750258/151‑1 (MQ=255)
           gcgcATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTg                                                                                                                                            >  4:1894845/1‑151 (MQ=255)
               aTAGAAATCCGCCATTATGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGc                                                                                                                                        >  2:243155/1‑151 (MQ=255)
               aTAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGc                                                                                                                                        >  8:249335/1‑151 (MQ=255)
               aTAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCCACTGCCGTACTGTCGc                                                                                                                                        >  6:272570/1‑151 (MQ=255)
                  gAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGcc                                                                                                                                       >  7:1792160/1‑149 (MQ=255)
                  gAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAg                                                                                                                                     <  4:753114/151‑1 (MQ=255)
                  gAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGcc                                                                                                                                       >  4:849961/1‑149 (MQ=255)
                   aaaTCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGcc                                                                                                                                       >  3:1272897/1‑148 (MQ=255)
                    aaTCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTACCGCCCGACTGGCGTACTGTCGcccgcc                                                                                                                                   >  8:1772952/1‑147 (MQ=255)
                       ccGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTtc                                                                                                                                >  4:1008890/1‑151 (MQ=255)
                          ccATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTcc                                                                                                                             >  1:1016560/1‑151 (MQ=255)
                           cATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGCCTTCTCCt                                                                                                                            >  5:1298441/1‑151 (MQ=255)
                              tAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGc                                                                                                                          >  5:1038664/1‑150 (MQ=255)
                                aGGGGCCGATAAACTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGAGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTACCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCACCTGCAt                                                                                                                        >  3:797936/1‑150 (MQ=255)
                                 ggggCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATc                                                                                                                       >  6:1605468/1‑150 (MQ=255)
                                  gggCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTCCCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCagc                                                                                                                       >  2:822833/1‑147 (MQ=255)
                                  gggCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAg                                                                                                                     <  5:871698/151‑1 (MQ=255)
                                     ccGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTg                                                                                                                   >  2:872571/1‑150 (MQ=255)
                                      cGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGACTCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTgag                                                                                                                 <  5:170604/151‑1 (MQ=255)
                                      cGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTgag                                                                                                                 <  6:1525306/151‑1 (MQ=255)
                                      cGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTAGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCCCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTg                                                                                                                   >  7:908893/1‑149 (MQ=255)
                                        aTAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGAc                                                                                                               >  1:1611732/1‑151 (MQ=255)
                                        aTAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGAc                                                                                                               <  1:449331/151‑1 (MQ=255)
                                        aTAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGAc                                                                                                               >  8:1490403/1‑151 (MQ=255)
                                          aaaGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACg                                                                                                              >  8:1930775/1‑150 (MQ=255)
                                          aaaGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGa                                                                                                             <  2:1279295/151‑1 (MQ=255)
                                            aGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAAt                                                                                                           <  4:2007698/151‑1 (MQ=255)
                                            aGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAAACTTCTCCTGCATCAGTGAGACGAAt                                                                                                           >  7:1379451/1‑151 (MQ=255)
                                              tAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCg                                                                                                         >  2:1403611/1‑151 (MQ=255)
                                               aGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGt                                                                                                        >  2:301850/1‑151 (MQ=255)
                                                   cGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGc                                                                                                    >  1:1404325/1‑151 (MQ=255)
                                                     tAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGccc                                                                                                  >  3:360599/1‑151 (MQ=255)
                                                     tAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGccc                                                                                                  >  7:1648273/1‑151 (MQ=255)
                                                        aGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCt                                                                                               <  5:68999/151‑1 (MQ=255)
                                                              gggTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTc                                                                                         >  2:363784/1‑151 (MQ=255)
                                                              gggTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTc                                                                                         >  8:1325136/1‑151 (MQ=255)
                                                               ggTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTc                                                                                         >  4:1612330/1‑150 (MQ=255)
                                                                  gACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAAc                                                                                     <  2:1936386/151‑1 (MQ=255)
                                                                   aCGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACa                                                                                    <  6:322672/151‑1 (MQ=255)
                                                                     ggCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGc                                                                                  >  6:1962322/1‑151 (MQ=255)
                                                                      gCGATACGCAGCGAGCGTCGCGTCAGATCGGCAACATCTTGATTCGCCCGTTTACCCGCCCCCAGTTCGTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCg                                                                                 <  7:554307/151‑1 (MQ=255)
                                                                           aCGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACg                                                                            >  4:947020/1‑151 (MQ=255)
                                                                            cGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCCGACGTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGc                                                                           >  3:1892020/1‑151 (MQ=255)
                                                                             gCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGcc                                                                          >  3:449578/1‑151 (MQ=255)
                                                                               aGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAATGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCt                                                                         >  2:990338/1‑150 (MQ=255)
                                                                               aGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTa                                                                        <  7:1971013/151‑1 (MQ=255)
                                                                                gCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTa                                                                        >  1:1236573/1‑150 (MQ=255)
                                                                                  gATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAAc                                                                     <  3:1752266/151‑1 (MQ=255)
                                                                                  gATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAAc                                                                     >  6:1768993/1‑151 (MQ=255)
                                                                                  gATCCTCGCGTCAGAGCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAAc                                                                     <  6:403559/151‑1 (MQ=255)
                                                                                   aTCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCTTACTGTCGCCAGACTTCTCCTGCATGAGTGAGACGTATCGTTCGACCGCTACGGTCAAACAGCGGCACGCCTAAAc                                                                     <  1:240371/150‑1 (MQ=255)
                                                                                    tCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAActc                                                                   >  2:1002259/1‑151 (MQ=255)
                                                                                      cTCGCGTCAGATCGGCAACATAATGACTAGACCGTGTAACCGCCCCCAGTTCCTGTACAGACCGACTGGCGTAATGTCGCCAGCCTTATACTGAATCAGTTAGACGAATCCATCGCCCGCTCCGGTAAACCAGAGGCACGCCTCAACtctc                                                                 >  6:72966/1‑151 (MQ=255)
                                                                                        cgcgTCAGATCCGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTctcc                                                                <  7:1120458/150‑1 (MQ=255)
                                                                                          cgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCctct                                                             >  5:1745133/1‑151 (MQ=255)
                                                                                          cgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCctct                                                             <  8:45402/151‑1 (MQ=255)
                                                                                          cgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCctct                                                             <  1:595962/151‑1 (MQ=255)
                                                                                          cgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCctct                                                             <  4:386490/151‑1 (MQ=255)
                                                                                               gATCGGAAACATCATGAATCGCCCGTTTACCCGCGCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGACAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTaagt                                                         <  3:627350/150‑3 (MQ=255)
                                                                                                aTCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTgg                                                       >  7:1206341/1‑151 (MQ=255)
                                                                                                aTCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTgg                                                       <  8:1611132/151‑1 (MQ=255)
                                                                                                    gCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCCCGCCTAAACTCTCCTCTAACTGGCGa                                                    >  1:838076/1‑150 (MQ=255)
                                                                                                    gCAACATCAGGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGaa                                                   <  7:790509/151‑1 (MQ=255)
                                                                                                      aaCATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATc                                                 <  7:1301443/151‑1 (MQ=255)
                                                                                                       aCATCATGACTCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATct                                                <  8:1009572/151‑1 (MQ=255)
                                                                                                           catGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATctgct                                             <  1:1846304/150‑1 (MQ=255)
                                                                                                             tGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATctgcagcg                                          >  3:1712686/1‑147 (MQ=255)
                                                                                                                 tCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGCGaaac                                      >  3:2024772/1‑150 (MQ=255)
                                                                                                                  cGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCGAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAgc                                     <  6:1201203/151‑1 (MQ=255)
                                                                                                                   gCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAgcg                                    >  2:632378/1‑151 (MQ=255)
                                                                                                                        tttACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCGAACTGGCGAATCTGCTGGGAAAGCGCAGGt                               >  5:1602052/1‑151 (MQ=255)
                                                                                                                        tttACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGt                               >  5:2020560/1‑151 (MQ=255)
                                                                                                                             ccGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGga                          <  1:1773837/151‑1 (MQ=255)
                                                                                                                              cGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTggg                           >  8:821729/1‑149 (MQ=255)
                                                                                                                              cGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGgag                         <  6:1427757/151‑1 (MQ=255)
                                                                                                                              cGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGgag                         <  3:1557867/151‑1 (MQ=255)
                                                                                                                               gCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGGTGGgaga                        <  8:1193052/151‑1 (MQ=255)
                                                                                                                                    cAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGc                   <  4:1752729/151‑1 (MQ=255)
                                                                                                                                      gTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCa                  >  4:1204556/1‑150 (MQ=255)
                                                                                                                                       ttCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAAc                >  7:449710/1‑151 (MQ=255)
                                                                                                                                       ttCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAACGCGCAGGTTGGGAGACGTGCAAc                >  8:332484/1‑151 (MQ=255)
                                                                                                                                         ccTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGc              <  3:257455/151‑1 (MQ=255)
                                                                                                                                          cTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGc              >  5:595975/1‑150 (MQ=255)
                                                                                                                                            gTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACt           >  6:988466/1‑151 (MQ=255)
                                                                                                                                            gTAACCCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCa             <  1:756139/149‑1 (MQ=255)
                                                                                                                                             tAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTg          >  4:1479111/1‑151 (MQ=255)
                                                                                                                                              aaCGGCCGACTGTCGTACTGGCGCCAGAGTTCTCGTGCATCAGTGAGTCGAATCGTTCGCGCTCTACGGTGAAACAGCGGCACGCCTAAACTCTCCTCTAACTCGCCAATCTGCTGTGAAAGCGCAGGTTGGGAGACGTGCAACGCACTg          <  8:1998494/150‑1 (MQ=255)
                                                                                                                                              aaCGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTgg         <  8:677110/151‑1 (MQ=255)
                                                                                                                                              aaCGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTg          <  6:1053628/150‑1 (MQ=255)
                                                                                                                                               aCGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggc        <  5:147296/151‑1 (MQ=255)
                                                                                                                                                cGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcg       >  2:605866/1‑151 (MQ=255)
                                                                                                                                                 gCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcgg      >  5:360325/1‑151 (MQ=255)
                                                                                                                                                 gCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcgg      >  2:1992503/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACG  >  NC_000913/358334‑358633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: