Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1450TC25.2% 365.3 / 144.8 246intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (64/96);  new base C (17/37);  total (109/137)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01

Only 100 of 274 total aligned reads displayed.

AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTA  >  NC_000913/2817997‑2818292
                                                                                                                                                       |                                                                                                                                                        
aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatt                                                                                                                                                         <  7:411300/150‑1 (MQ=255)
 gTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAattta                                                                                                                                                       <  1:540505/151‑1 (MQ=40)
   gccttctttaaaTTCCGCAACCTATTCGAAACCTTCATAGATCCATATATAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAacttact                                                                                                                                                     <  8:1690544/141‑3 (MQ=14)
   gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttat                                                                                                                                                      <  6:620050/150‑1 (MQ=255)
   gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAaattaat                                                                                                                                                     >  3:1689188/1‑149 (MQ=255)
   gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTAGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAaattaat                                                                                                                                                     >  3:1892063/1‑149 (MQ=255)
    ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAaattaatt                                                                                                                                                    >  7:1095451/1‑151 (MQ=255)
     cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAattt                                                                                                                                                   >  1:658117/1‑151 (MQ=255)
      tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCAGAGGACACTCTCCCCCGGAGATACCGATGCATCTGG‑AAagttagagtt                                                                                                                                                  >  4:198885/1‑146 (MQ=14)
       gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTAc                                                                                                                                                 <  8:121371/151‑1 (MQ=255)
         ggTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTActg                                                                                                                                               >  3:74701/1‑151 (MQ=255)
            tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                             <  2:232513/150‑1 (MQ=255)
            aatttacGCTACATCTTCCATCCCATCATACCGCAAGATCTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTActgca                                                                                                                                             <  8:461418/144‑2 (MQ=11)
               ttccgcgagatattCCATACCTTCCTTGCTGAAGATCTCGGACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                        <  5:292031/138‑1 (MQ=11)
               ttactcTACCTATTCCTTACCTTCATTTAGGAAGATATAGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                        <  7:363170/145‑1 (MQ=11)
                  cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGTGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAactt                                                                                                                                                        >  3:893218/1‑133 (MQ=255)
                     caCCTCAGCGACACCCTCATTGCCCCACACTGCGGAGAATATGGTTCATCCGGGAGGATTCGAACCTCCGACCGCG‑‑CGGTTCGTACCCGAGTACTCTATGCAGCTGAGCGACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATa                                                                                                                               <  8:1923931/150‑1 (MQ=14)
                       tacgatgtaaaCCTTCATACATGCAGATCACGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttga                                                                                                                                 <  7:155040/139‑5 (MQ=11)
                        ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATT‑‑tataa                                                                                                                              >  6:1774584/1‑148 (MQ=255)
                        ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAATTTACTGATGa‑‑tatgaacgacg                                                                                                                              >  5:809048/1‑143 (MQ=14)
                        ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTC‑CTCCCGGGGCCGCTACCCGTGCG‑AGCCGAGTACGCAAACCAGCTGACCTACGGATGCTTACCG‑AAAGTTATTTTCGTTGTGA‑‑TATT‑‑gtgcgtg                                                                                                                            >  2:1261466/1‑145 (MQ=2)
                            tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATTCATCTGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGc‑t                                                                                                                         <  3:606728/149‑1 (MQ=255)
                             ccaTACCTTCATTCCTGAATATGTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGc‑t                                                                                                                         <  8:944188/148‑1 (MQ=11)
                             cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCCACCCTGAGCCCGTA‑‑CGGGTGCTCGCCGATGACTCTATCCAGCTGAGCTACGGAGGCATCGGC‑AAAATTAAAGTACTGCTCA‑‑TTTT‑‑TATACCgagat                                                                                                                        >  8:638924/1‑146 (MQ=2)
                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑ttttttgatcgcgagta                                                                                                                        >  2:1814654/1‑137 (MQ=14)
                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑TTTT‑‑tatatacctaata                                                                                                                      >  7:1165153/1‑141 (MQ=11)
                                tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TTTT‑‑GTTAgcgcgagact                                                                                                                     >  4:75291/1‑144 (MQ=14)
                                 aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTaaa                                                                                                                   <  2:331245/150‑1 (MQ=255)
                                 aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTaaa                                                                                                                   <  6:536240/150‑1 (MQ=255)
                                 aCCTTCATTGCTGAAGATTTCGGAGAATATGGCGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGc‑tacta                                                                                                                     <  1:1060246/150‑1 (MQ=11)
                                   cgtcctagcgccaGATTTAGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGC‑TACTAAAg                                                                                                                  <  8:1469424/139‑1 (MQ=14)
                                   cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATAAAGC‑TACTAAATcc                                                                                                                >  6:836503/1‑151 (MQ=255)
                                    gtaattgagcaataTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGC‑TACTAAAGc                                                                                                                 <  6:1514092/138‑1 (MQ=11)
                                     tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGATACGGATCCATGCGG‑AAAATTAAATTACTGATCA‑‑AATA‑‑GATAACGC‑TACTAAAGccc                                                                                                               >  1:950661/1‑149 (MQ=11)
                                      cATTTCTGAATATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCata                                                                                                             <  1:186017/151‑1 (MQ=255)
                                      cATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCGGa‑‑t                                                                                                                                        >  5:248649/1‑127 (MQ=255)
                                       aTTGCTGAATATTTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGC‑TACTAAAGccgt                                                                                                              <  4:1228183/151‑3 (MQ=11)
                                         ttcTGAATATTTCGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGC‑TACTAAAGCCgta                                                                                                             <  5:75138/148‑1 (MQ=14)
                                         tGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATc                                                                                                           <  3:1467163/150‑1 (MQ=25)
                                         tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATT‑‑GAGAGCGC‑GCGTAAAGCCCTATc                                                                                                           >  6:786228/1‑150 (MQ=14)
                                         acaggAAGATCTAGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTAAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGC‑TACTAAAGCCgta                                                                                                             <  3:233276/146‑1 (MQ=14)
                                             gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGt                                                                                                       <  2:991238/150‑1 (MQ=255)
                                             gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATTTAGATACCGA‑TAAAAAAGCCCTATaaat                                                                                                        >  8:681314/1‑150 (MQ=14)
                                             gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATT‑‑TAGACCGC‑TCCTATAgagctctctaga                                                                                                       >  6:347481/1‑139 (MQ=14)
                                             caatatttagtactatatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGC‑TACTAAAGCCGTATCaa                                                                                                         <  7:1814504/136‑1 (MQ=17)
                                               aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAag                                                                                                    <  5:1078357/151‑1 (MQ=255)
                                               aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑‑GATACCGc‑gact                                                                                                                      >  2:1376515/1‑135 (MQ=11)
                                                  ttacgtatattttGGTGCTTCCGGGAGGATTCGAAGGTCCGACCGCT‑‑GGTTTGGTAGCGGAGTACTCTTTCAAGTTGAGCTAGGGATGCATCGGT‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAagag                                                                                                  <  3:1128635/138‑1 (MQ=14)
                                                   ttCGGAGAATATGGTGCATCCGGGAGGATTAGACCCTCCGACCGGG‑‑CGGTTCGTGGCCGAGTCGTCTATCAAGCTGAGCTACGGATGGCGCGGG‑GTATTTAGTTTTCTGCTGA‑‑TATT‑‑GATACCGC‑TCGTAACGccccttaaagtatg                                                                                                    >  8:819801/1‑135 (MQ=9)
                                                    tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGAt                                                                                                <  6:1084965/150‑1 (MQ=40)
                                                     ggtcgtattcggTGCTTCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATgg                                                                                              <  4:308935/141‑1 (MQ=255)
                                                      gtataaTATGGTCCATCGGGTAGTATTCTAACCTCCGACCGCT‑‑GGGTTCTTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGT‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGt                                                                                             <  3:1810939/147‑1 (MQ=14)
                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGt                                                                                             <  6:760676/151‑1 (MQ=39)
                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGt                                                                                             <  7:1729139/151‑1 (MQ=39)
                                                       gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                            <  3:468610/151‑1 (MQ=39)
                                                       gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                            <  8:1861158/151‑1 (MQ=39)
                                                       gagaATATGCGGCCTCCGGGAGGAGTCGAACCTCCGACCGCT‑‑CGGATCTTTTCTTATTAATCTCTCCAGGTGAGCTGAGGATGCATCGGG‑TTAGTTTTGTTAATACTGT‑‑TAGT‑‑‑CTTCCGCGTTCTAAAGCCATATCAAGTAAGAGATGGTg                                                                                            <  8:388455/151‑1 (MQ=255)
                                                          aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATGTATTTTACTTGGGA‑‑TTAT‑‑TATTCCGC‑TAATAAAGCCATATCAAGTAagaccaggtagg                                                                                          >  2:153142/1‑141 (MQ=14)
                                                             atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                       <  2:558576/150‑1 (MQ=40)
                                                              tGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTAACTGCTGA‑‑TAAT‑‑TATACCCC‑TAAGAAAGCCATATAAAATAAGAGAttagttccga                                                                                       >  5:1274677/1‑140 (MQ=11)
                                                                   cATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCGGA‑‑GATT‑‑GAGAGCCC‑GAGAAAAGCGATCTCAACTTATAGATGTTgaaacacggcggg                                                                                >  2:770621/1‑139 (MQ=11)
                                                                    aTCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAt                                                                               <  1:850122/151‑1 (MQ=39)
                                                                    aTCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAACAGATGGTGCATCCGGGAGGAt                                                                               <  7:750849/151‑1 (MQ=255)
                                                                    aTCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATT‑‑TATAGCGC‑GACTCAAGCCATTTCTAGTATCAGATGGTGCATcaccgtgcat                                                                               >  6:187898/1‑142 (MQ=14)
                                                                      ccGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAat                                                                                                                                                          >  4:1614929/1‑79 (MQ=31)
                                                                       ccggaggcTTCCAACCTCCGCCCGCG‑‑CCGCGCGTAGCCGAGGACTCTATCCCCCACTGCTACCTCTGCAGCGGG‑AAATTTAAATTCCTCCTCA‑‑TATT‑‑CCAACCCT‑TCCTACCTCCATATCAAGGACCATATGGTGCATCCGGGAGGATTCg                                                                            <  5:988739/149‑1 (MQ=255)
                                                                        gggAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATT‑‑TATAACGC‑TACTAAAGCCATATCAAGTAAGAGATGTTGAATCAggggatatacg                                                                            >  5:1203814/1‑142 (MQ=14)
                                                                        gggAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAATTTACTGCAGA‑‑TATT‑‑TATAAAGC‑TAAAAAAAACAAATAAACTAAAAGATAGTgaaaaagcgagactt                                                                              >  7:1409442/1‑134 (MQ=2)
                                                                         ggtggaTTGGAACCTCCGAGGGCG‑‑CGGTTCGTAGCCGCGTTCGCTATCCTGCTGCGTTACGGATGCATCGGG‑ATATTTTTTTTACTGGTGA‑‑TCTT‑‑GGTAACGC‑TACTTCCGCCATATGAAGTACGATATGGTGCATCCGGGAGGATTCGaa                                                                          <  1:1652660/148‑1 (MQ=9)
                                                                                  gAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑TATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGAATCAGCGAGGATTAGacgcgcagacc                                                                 >  7:666498/1‑141 (MQ=14)
                                                                                       gccGACCTCT‑‑CGGATAGTAGACGAGTACGCTATCCAGATGTTCTTCGTATCCAGCGGG‑ATATTTATTTTACTGCTGA‑‑TATT‑‑GCTACCGT‑TACTTCATCAATATAAAGTACCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                            <  5:1390308/150‑1 (MQ=16)
                                                                                        ccGACCGCT‑‑CGGAGCGTAGCCGAGTACGCAATCCAGCAGAGCTACGTAGGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACCACAGCCATATCAAGTAACAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                           <  6:1467994/151‑1 (MQ=17)
                                                                                          gACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                       <  1:398649/76‑1 (MQ=255)
                                                                                          gACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGc‑tact                                                                                                                      <  6:1615915/90‑1 (MQ=255)
                                                                                          gACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑AATT‑‑TATACCGc‑taat                                                                                                                      >  1:1615130/1‑88 (MQ=255)
                                                                                             cGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCGCCGAcccgcgcgctcgt                                                      >  4:78715/1‑140 (MQ=14)
                                                                                              gCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGAGCGTGAATCCGGGAGGATTAGACTCTCCGACCggctcgctcgtc                                                     >  6:209064/1‑140 (MQ=14)
                                                                                                      ttCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATa                                                                                                                               <  5:944109/72‑1 (MQ=255)
                                                                                                         gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TAATAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATAGGAACAGCCCACCGCTCTGATCgactcacaggac                                            >  4:1335817/1‑140 (MQ=14)
                                                                                                               gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAGCTCCGACCGGTCGGTTCGTAGCCGAGTACTCTAt                                       >  3:438510/1‑150 (MQ=255)
                                                                                                                aGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                            >  1:1404146/1‑53 (MQ=255)
                                                                                                                   aCTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATAACGC‑TACTAAAGCCATATCAAATAAGAGATGGTGAAACCCGGAGGATTCTAACCTCCGACCCCAAGGTTAGTAGCAGAGTaaagaaagcaa                                   >  1:1331918/1‑140 (MQ=17)
                                                                                                                    ctctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACGCTCCGACCGGACCGATCGTAGCCGAGTAGTCTATCCAGCt                                 >  8:1059756/1‑151 (MQ=18)
                                                                                                                      caaTCCAGCTCACGTACGGATGGATCGGG‑AAATTAATTGTGCAGCTGA‑‑TAAT‑‑GATACGGC‑TACTTTAGCCAGATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                <  6:951496/148‑1 (MQ=18)
                                                                                                                       tATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                            >  4:960067/1‑46 (MQ=255)
                                                                                                                       tATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACTCTCCGAGCGGCGCGCTCGTCGCCGAGTagaaaatcccgctga                               >  1:809200/1‑136 (MQ=14)
                                                                                                                            acgctcttcttatgtGCATGGGGAAAAGTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                          <  5:618228/137‑1 (MQ=21)
                                                                                                                            aGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGAATCCGGGAGGATTAGACGCTCCGAGCGGAGCGCTCGTAGCCCAGTAGTATAGCCAGCTgtcctccc                         >  3:1068202/1‑144 (MQ=14)
                                                                                                                              cTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACGGGGGA‑‑TATT‑‑GATACGTC‑TACTAAAACCATATAAAGAAAGAGATGGTGCATCCGGGAGGATTCGAACATAAAAAAGATCGGTTCGTAGCGGAGTAGTCTATCCAGCTGAGCTCCggg                       >  7:900400/1‑150 (MQ=16)
                                                                                                                               agatctacctacgtaTCGGG‑ATATTTATTTTACTCCTGA‑‑TATT‑‑CTAAACGA‑TCCTACCTGAATATTAAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                      <  1:253162/137‑1 (MQ=2)
                                                                                                                                      cGGATCCATCGGC‑AAATGTTATTTCCTGCTGA‑‑TATT‑‑CCTTACCC‑TACTACATCAATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg               <  8:589281/151‑1 (MQ=17)
                                                                                                                                       ggATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAGCTCCGACCGACCGGTTCGTAGCCGAGTACTCTATACAGCTGAGCTACGGCTGCATCggg              >  8:78300/1‑151 (MQ=255)
                                                                                                                                           gCATCGGGAAAACTTATTCTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACAACCTCAGACCCAACCCTTACTAGCCGATTACTATATCAAGCTGAGATAcatatagcaagagca            >  4:618855/1‑136 (MQ=2)
                                                                                                                                             aTCGGGAAAACTTATTATACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                             <  6:1288479/131‑1 (MQ=255)
                                                                                                                                             aTCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACCCTAGGACCCTACCGATAGTCGCCGATTAGTCTATCAAGCTGAGCTACGGAGGGATCGCGCAactt        >  8:1017343/1‑151 (MQ=17)
                                                                                                                                              tCGGGAAAACTTATTCTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCAGAccc                                                                >  4:1728285/1‑94 (MQ=11)
                                                                                                                                              tCGGGAAAACTTATTCTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCg                                                                <  5:1726745/95‑1 (MQ=11)
                                                                                                                                              tCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACCCGGGGCGCGGGGGGGTGGTCGGCGCGTGATCTATCCAGCTCAGCTAAGTCTGCATCGGCTAactgc       >  8:1741806/1‑149 (MQ=9)
                                                                                                                                               cGGT‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                 <  6:445232/140‑1 (MQ=255)
                                                                                                                                               cGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                 >  1:445489/1‑140 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCT‑‑CGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGC‑TACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTA  >  NC_000913/2817997‑2818292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑