Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1510AC26.9% 229.8 / 50.1 256intergenic (+549/+474)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base A (105/72);  new base C (63/2);  total (180/76)
Fisher's exact test for biased strand distribution p-value = 5.22e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270011‑3270301
                                                                                                                                            |                                                                                                                                                        
cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCAACAAAACCAAAAACCCCCCGAAGCCCCTTTTTACGTAAACCACGTGAAACTTACCCATAAACAGCGTTCTGTTGGGGAAAGAGACGTCGAA‑AGtgcatc                                                                                                                                                >  5:1568236/1‑145 (MQ=2)
    ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTGTGCCCCTCCAAAACCAAAAAACCGCCGCAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAATCCGGGTTCTTTTGGGGATAGCGTTTTGGCA‑AGGCATTCATAtt                                                                                                                                           >  2:475842/1‑151 (MQ=11)
     tctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGGTTCTTTTGGGGATAGCGTAGTGGCC‑acgcaatcaatatt                                                                                                                                          >  6:841915/1‑138 (MQ=11)
               ataGCGTCGTGGACAGTTTTTCATCTTTATTCCCCTCCAACAGCAAAAACCAGGCGTATCTGGTTTTTACGTAAACAAGTTGATATTGAGATATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTcc                                                                                                                                <  5:651638/151‑1 (MQ=11)
                 cgcccccgccccAGGCAATCAGCTTTCACCCCCTCCAAAAGCAAAAACCCGCCTAATCTGTTTTTTACGTAAACAACGTGAAACTGACCCCTACCCCGCTTTGTTTTCGGTATAGCTTCGTCGAC‑AGTCATTCATCTTTCTGCCCCTCCaa                                                                                                                              <  8:517571/140‑1 (MQ=2)
                                 attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTGGGGTATAGCGT‑ATGGTCAAGTCATTCAAATTTTTGCACCTGCAAAACCAAAAAAcccccg                                                                                                              >  1:1310577/1‑151 (MQ=11)
                                 attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCCTATTTGCG‑AGTGATTCATATTTCTGCCCCAACAAAAACAAAAAAacgccc                                                                                                              >  8:489340/1‑150 (MQ=11)
                                  ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTAGAGCGTCGTGGCG‑AGGCCTGCATGGTTGGGCCCCGGCAACCGCAAAAACccgccgc                                                                                                             >  6:599253/1‑150 (MQ=11)
                                        tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTCGAG‑AGGCATGCATGTTTTGGCGGCGCCAAAACCAAAACCCCGCGGCAG‑Cggg                                                                                                      >  6:1059483/1‑151 (MQ=11)
                                            tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGCC‑AGTCCTTCAAGTTTCTGGCCCGGCAAAAGCCAAAACCCGCCGCAG‑CGCGtgtt                                                                                                  >  6:1744696/1‑151 (MQ=11)
                                             gcccctccaccccccaaaaCCCGCCGACCCGGGTATTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTGTCTTTTGCGTATAGCGACGTCGAC‑AGGCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCGAAG‑CGGGttttt                                                                                                 <  6:492686/136‑1 (MQ=11)
                                               cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGTGTAAAGCCACGAGGCA‑AGTCAATCAAAATTAAACGCCAACAAAAGCAAAAAACCCCCGAAG‑AGGGAATTTa                                                                                                >  1:433662/1‑150 (MQ=2)
                                                ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGTCCTTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑Ccgggtggttcg                                                                                              >  2:338894/1‑143 (MQ=11)
                                                 cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCG‑AGTCCTTCATGGTTGTGCCCCGGCAACAGCAAAAACCCGCCGCAG‑Cggggggtttcgg                                                                                             >  6:1131768/1‑142 (MQ=11)
                                                   ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGTCCTTCATAGTTCGGCCCCTGCAAAACCAAAAACCCGCCGAAG‑GGCGttggttcgga                                                                                            >  1:1053555/1‑142 (MQ=11)
                                                    cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGAA‑AGGCAATCAAAATGAAGCCCCGACAAAAAAAAAAACAAGCCCAAA‑CTGGTGTTTACGTaa                                                                                           >  5:532657/1‑150 (MQ=2)
                                                     aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGCA‑AGGCATTCAAATTTATGCCCCAACAAAACCAAAAACCCGCCGCAG‑CCGGTTGTTAACTAAAt                                                                                         >  3:1869477/1‑151 (MQ=11)
                                                      aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCG‑AGTCCTTGATGTTTGTGCCCCTGCAAAAGCAAAAACCCGCCGCAG‑GGGGGTTTTaaggtaatc                                                                                        >  8:1458032/1‑143 (MQ=11)
                                                       aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGTCAATCATCGTTCTGCCCCAACAAAAGCAAAAACCCGCCGAAG‑AGCGTTTTTACCTAAATc                                                                                        >  1:1779640/1‑150 (MQ=11)
                                                            gaaaCCCGCCGTATCGGGTTTTTACGTAAATCAGGTCAATCTGACCGATAAGCCTCTTTCTTTTGGGTATAGGGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAg                                                                                      <  5:715133/146‑1 (MQ=11)
                                                                cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGTCATTCATGTTTGGGCCCCGGCAAAACCAAGAACCCGCCGCAG‑GCGGGTTGTAAGTAAATCAGGTGCAACt                                                                              >  4:352499/1‑151 (MQ=11)
                                                                cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGTCATTCATCTTTGTGCCCCGCCAAAAGCAAAAACCCGCCGAAG‑CGGGGGTTTAAGTTAATGAGGGGCAACt                                                                              >  2:1667383/1‑151 (MQ=11)
                                                                cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCA‑AGTCATTCATGTTTCTGCCCCTCCAAAACCAAAAACCCGCCGAAG‑AGGGGTTTTACGTAAATCAGGTGaaaat                                                                              >  2:849427/1‑149 (MQ=11)
                                                                 ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGC‑AGGCCATAAACATTATGCCCCCCCCAAAACAAAAACACCCCCCAG‑CGGGTTTTTAAGGAAATGACGTGaaaatc                                                                             >  8:664542/1‑148 (MQ=2)
                                                                     cgtaacGGTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAg                                                                                      <  5:374778/133‑1 (MQ=11)
                                                                     cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGGCATTCATCGTTGTGCCCCGGCAAAAGCAAAAACCCGCCGCAG‑GGGGGTTTTACGTAAATCAGGTGCAACTGAccc                                                                         >  6:1579661/1‑150 (MQ=11)
                                                                     cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGCG‑AGTCCTTCATAGTGGTGCCCCCCCAACCACAAAAACCCGCCGCAG‑CCGGGGGGTACGTAAATGACGGGCAACTGGccc                                                                         >  4:1644615/1‑150 (MQ=2)
                                                                           gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTAATTCATATATCAGCCCAAACAAAAACAATAACCCCCAGAAC‑AAGGTTAATAACTAAATCAAGGGCAACTTCCAGATAAGc                                                                   >  7:1313280/1‑151 (MQ=2)
                                                                            ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGTCCTTCATCTTTGGGCCCCGCCCAAACCAAAAACCCGGCGAAG‑CGGGGGTTTACGTAAATGAGGGGCAACTTCCCCTTAAGcc                                                                  >  6:718577/1‑151 (MQ=11)
                                                                             gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCA‑AGGCATTCAAAGTTGTCCCCCGCCAAAACCAAAAACCCCCCGAAGCCCGGGTGTTAC‑TAAATCACGGGCAACTGACCGATAATCCg                                                                 >  4:1041655/1‑151 (MQ=2)
                                                                               ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCG‑AGGCATTCATGGTTGGGCCCCAGCAAAACCACAAACCCGCCGCAG‑CCCGGGGTTACGGAAATCAGGGGCAACTGGCCGATAAGCCggg                                                               >  7:1474536/1‑151 (MQ=2)
                                                                               ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAA‑AGTCCTTCATGTTTATGCGCCTACAAAAGCAAAAACCCGCGGCAG‑AGGGTTGTTACGTAAATCAGCTGAAAGTTAGCGATAATACggg                                                               >  3:1532418/1‑151 (MQ=11)
                                                                                   aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAG‑AGTCATTCATGTTTGTGCCCCGCCAAAAGCAAAAACCCGCCGAAG‑CGGGGTTTTACGTAAATGAGGGGCAACTGCCCGATAAGCCGGGTTCt                                                           >  4:1764333/1‑151 (MQ=14)
                                                                                    cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATATTTGTGCCCCTGCAAAAGCAAAAACCCGCCGAAG‑CCGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTg                                                          >  2:1146104/1‑151 (MQ=255)
                                                                                    cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAA‑AGGCATTCATAATTGGGCCCCAGCAAAAACAAAAACCCGCCGCAGACCGGTTTTAAC‑AAAATCACGGGCAAATTGCCCATAAGCCGGGTTcat                                                          >  3:84491/1‑149 (MQ=2)
                                                                                     gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATATTTGTGCCCCGCCAAAAGCAAAAACCCGCCGAAG‑CGGGGTTTTTCGTAAATCAGGTGAAACTTACCGATAAGCCGGGTTgtgt                                                         >  4:1344809/1‑151 (MQ=14)
                                                                                      tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAG‑AGTCATTCATCTTTGTGCGCCGCCAAAAGCAAAAACCCGCCGCAG‑CGGGGTTTTACGTTAATGAGGGGCAACTGACGGATAAGCCGGGTTgtgtg                                                        >  3:357021/1‑150 (MQ=14)
                                                                                      tAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGG‑AGTCAATCAATATTGAGCCCGAACAAAACCAAAAACCCGCCGCAGCCCGGTGTTTACGAAAAGACGGCCAAA‑TTCCCGATAATCCGGGTTgtgtt                                                        >  2:513334/1‑150 (MQ=2)
                                                                                       caaccccgcgAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTTGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAACCCCGCCGAAG‑CGTGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGGGTTCTGt                                                         <  2:1508371/140‑1 (MQ=11)
                                                                                       aaatgacgttaatcTGAGCGATAAGCCGCTTTCTTTTGCGTATCGTGTCGTGGAA‑TGTGCATCATATATCTCCCACTCCAAAATCAAAAACCCGCCGCAG‑CGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                                        <  8:835601/137‑1 (MQ=2)
                                                                                       aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGGGTCGTGGCG‑AGTCATTCATCGGTGAGCCCCTGCAAAAACAAAAACCCGCCGCAG‑CCGGGGTTTAAGGAAATGAGCGCCAACTGACCGATTAGCCGGGTTgtgtgg                                                       >  7:1137764/1‑149 (MQ=2)
                                                                                         aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGGCATTCATAATTGCGCCCCGACAAAAACAAAAACCCGCCGCAG‑ACCGTGATTAAGTAAATCACGGGATACTGGCCCAAAAGCCGGGTTCTGtaggg                                                     >  1:225523/1‑147 (MQ=9)
                                                                                           cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑CGGGGTTTTACGTAAATCACGTGAAAATGACCGATAATCCGGGTTCTGTCggggg                                                   >  2:5699/1‑150 (MQ=14)
                                                                                            aGGTGAAACTGGCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTC‑AGTAAATCATCGTTATGGCCCACCAAAATCAAAAACCCGCCGAAG‑CGGGTTTTTAAGTAAAAAAGGTGAAAATTAACGATAAGCCGGGTTCGGtggggggg                                                  >  3:1474138/1‑144 (MQ=2)
                                                                                            aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGGCATTCATGGTTGTGCCGCTACAAAAGCAAAAACCCGCCGAAG‑GGGGGGTTTTCGTATATCAGGTGAAACTGGCCGATAAGCCGGGTTGTGtggtgggg                                                  >  1:1214048/1‑149 (MQ=14)
                                                                                             ggTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTAGTGTCA‑AGTCATTCATGTGTTGGCCCCGCCAAAATCAAAAACCCGCCGAAG‑CCCGGTGGTAAGCAAATCAAGTGAAAATTTCCCTTTATCCGGGTTCTGTCGTgggac                                                 >  2:695344/1‑148 (MQ=2)
                                                                                               tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCCTGGAC‑AGTCAATCATCTTTCAGCGCCAACAAAAGCAAAAACACGCCCAAC‑CGGGTTTTTACGTAAATCTGGTGAAAGTGTCCGAAAAGCCGGGTTCTGtactggcccgg                                               >  6:1126321/1‑141 (MQ=14)
                                                                                                gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATAGTTGTGCGCCGCCAAAAGCAAAAAACCCCCCCAG‑CCGGGTTTTACGGAAATCACGGGCAACTTCCCGCTAAGCCGGGTTCTGTGGTGGGCAGtc                                              >  8:1192222/1‑151 (MQ=9)
                                                                                                  aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGTCA‑AGTCAATCATGGTTGGGCCCCGTCAAAAACAAAAACCCGCAGCAG‑GGGGGTGTTAACTAAAACACGGGAAAGTGGCCCATAATCCGGGTTCttatgcgtaaacgga                                             >  2:1781485/1‑136 (MQ=2)
                                                                                                   ccTGCCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCATCCAAAAGCAAAAACCCGCCGAAC‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcatt                                           <  8:1475113/150‑1 (MQ=255)
                                                                                                    cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCC‑CGTCCTTCAGCTATCAGCCCCAACAAAACCAAAAACCCGCCCAAC‑CGCGGTATTACGTAAATCAGGGGACACAGACCGAAAAGCCGGGATCTGACGACGACAGtcata                                           >  7:1037835/1‑149 (MQ=9)
                                                                                                     tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTATATGCCCCTACAAAAGCAAAAACCCGCAGAAG‑CCCGTTTTTAACTAAATCAAGTGAAACTGAACCATAAACCGGGTTATGTCGTGGTAAGtcattc                                          >  1:747299/1‑150 (MQ=14)
                                                                                                     tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCACGTGAAACTGACCGATAAGCCGGGTTGTGTCGTGGCCAGtcattc                                          >  8:1833649/1‑150 (MQ=255)
                                                                                                     cgcttgctatctcGCTTTCTTTTGGGTATAGTGTCGTTGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑TGTGTTTTTACGTATATCAGGTGAAACTGACCGATTAGCCGGGTTCTGTCGTGGACAGtcattca                                         <  3:229290/139‑1 (MQ=14)
                                                                                                     cgACCGATAAGCCGCTTTCTTTTGCGTATAGGGTCGTTGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattca                                         <  2:1270512/150‑1 (MQ=255)
                                                                                                       aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCACGTGAAACTGACCGAAAAGGCGGGTTATGTGGTGGGCAGTCATTAATc                                       >  1:1503174/1‑151 (MQ=14)
                                                                                                       aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAG‑CCGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                                       >  5:1337922/1‑151 (MQ=255)
                                                                                                        ccGATACGCCCTTTTCTTTTGGGTATAGTGTCGTTTAT‑ATTTATTCATCTTTCTGCCCCTCCAAAAGTAAAATCCGGTCTAAT‑CTGTTTTTTAAGTATATGATGTTAATATGATTTATATTGGGTTTTGTGTCGTGGACAGTCATTCATCt                                      <  5:1135933/151‑1 (MQ=0)
                                                                                                        ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑ACTCATTCATCTTTGTGCCCCTCCAAAACCAAAAACCCGCCGCAG‑CGGGTTTTTACCTAAATCACGTGAAACAGACCGATAAGCCCGGATCTGTCGTGGACACTCATTCATc                                       >  4:306133/1‑150 (MQ=17)
                                                                                                         cGATCAGCCGCTTGCTTTTGGGTATAGGGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTGCAAAAGCAAAAACCCGCGGAAG‑CTGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                                     <  6:268889/151‑1 (MQ=17)
                                                                                                         cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑AGTCATTAATCTTTCACCCCCAACAAAAACAAAAACCCGCCGAAT‑CGGGTTTTTAAATAAATAAGATGAAACTGAACCATAAGCCGGTTTCAGTAGTgtaaataaaaaagtaa                                      >  1:1640462/1‑135 (MQ=2)
                                                                                                         cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGC‑AGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAG‑CCGGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCCGGTTCTGTGGTGGGCAGTCAATCATCt                                      >  3:69887/1‑150 (MQ=14)
                                                                                                         cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTAATATTTGATCCCCTCCAAAAGCAAAAACCCCCCGAAG‑CGGGGTTTTACCTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCAGTCGTGCACAGGAATTAATCt                                      >  5:1024948/1‑150 (MQ=14)
                                                                                                          ccgatggcGCTTTCTTTTGGGTATAGTGTCGTGTAC‑ATTCTTTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATATGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                                    <  7:596016/144‑1 (MQ=17)
                                                                                                           aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGC‑AGTGATTCATGTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGCAG‑CGGGTTTTTACGTAAATGAGGTGAAACTGACCGATAAGGCGGGTTGTGTGGTGGTCAGTGATTCATCTAgg                                   >  7:1800000/1‑151 (MQ=17)
                                                                                                           aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAgg                                   >  5:1473578/1‑151 (MQ=255)
                                                                                                           aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTAATCTTTCTGCCCCTCCAAAACCAAAAACACGCAGAAG‑CCGGGTTTTACGTAAATAAGGTGAAACATACCGATAAGACGGGTTCTGTCGTGGACAGTCATTCATATAgg                                   >  1:132840/1‑151 (MQ=17)
                                                                                                            tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑AGTCATTCAAATTTGTGCCCCAACAAAAAAAAAAAACCCCCGAAG‑CGGGGTTTTAAGTAAAAAAGGTGAAACTGACCGATAAGAACCGTTGTGTGGTGGAGAGtgagtgggagagg                                   >  7:1499877/1‑138 (MQ=2)
                                                                                                             aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGAC‑AGTCATTCATCTTTCAGCCCCAACAAAAAAAAAAACCCGCCGAAG‑CGGGGATATTCGTAAAACAAGTGAAACTGAACGAAAAGCCGCGTTATGTGGGTGACAGtgaaaaaaaaaaaa                                  >  5:286945/1‑137 (MQ=2)
                                                                                                             aaGCCGCTTTCTTTTGGGTATAGGGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGcc                                 <  8:699440/151‑1 (MQ=255)
                                                                                                              cgCCGCTTTCTTTTGGGTANAGCGTCGTGTAC‑AGGAATTCATCTATCTCACCCTCCAAACGGACAACGGCCGGGACG‑TCTGTTTTTAGGTAAATCCCGATAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                                <  6:944159/150‑1 (MQ=16)
                                                                                                              aGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAAATGACCCAAAACCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGccc                                >  2:985161/1‑150 (MQ=255)
                                                                                                                ccGCTTTCTTTTGGGTATAGCGTCGTGGCC‑AGTCATTCATATTTATGCCCCTCCAAAACCAAAAAACCGCCCAAG‑CGGGTTTTTAAGTAAATCACGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCATTCATCTAGGCCacc                              >  6:1911062/1‑149 (MQ=18)
                                                                                                                 cGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGAAAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                              >  5:236339/1‑150 (MQ=255)
                                                                                                                   ctttctttTGGGTATAGTGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCg                                                       <  2:241576/123‑1 (MQ=38)
                                                                                                                   ctttctttTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                           >  4:1169942/1‑151 (MQ=255)
                                                                                                                     ttctttTGGGTATAGTTTCGTTTAA‑AGTCATTCATCTTTCTGCCCATCCAAAAGGAAAAACCCGCCGAAG‑CGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAATGCGTTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                           <  5:792528/149‑1 (MQ=17)
                                                                                                                     ttctttTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg                         >  1:559009/1‑151 (MQ=255)
                                                                                                                      tctttTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTAGGGGAGAGTGATTAAACTAGGCCAGCAATAGc                        >  7:1962/1‑151 (MQ=14)
                                                                                                                       ctttTGGGTATAGTGTCGTTGAC‑TGTTTTTAATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑AGTGTTTTTACGTAAATCAGGTGAAACTGATCTATAATGGGTTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                          <  7:1901832/148‑1 (MQ=17)
                                                                                                                       ctttTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGTCAGTCATTCATCTAGGCCACCAATCGcg                       >  2:1493660/1‑150 (MQ=255)
                                                                                                                         tttGTGTATAGCGTCGTTCAC‑AGTCATTCATCATACTTCCCCTATCATAGCAAAAACCCGCCGAAT‑CGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTCTTCTAGGCCAGCAATCGCTc                      <  3:721066/150‑1 (MQ=255)
                                                                                                                         tttGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTcc                     >  4:1108251/1‑150 (MQ=255)
                                                                                                                         tttGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTATGCCCCTCCAAAAACAAAAACCCCCCGAAC‑CGGGTTTTTACGTAAATAAGGTGAAAATGACCGAAAAACCGGGTTCTGTCGTGGGCAGTCAATAATCTAGGCCAGCAATAGCTc                      >  3:47344/1‑150 (MQ=18)
                                                                                                                          ttGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAACCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa                     <  4:1807541/150‑1 (MQ=255)
                                                                                                                           tGGGTATAGTGTCGTGGAC‑AGTCATTGATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑CGGGTTTTTACTTAAATCACGTTAAACTGACCGATAAGCCGGCTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCAc                    <  6:1641331/150‑1 (MQ=18)
                                                                                                                           tGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGGCGTGGACAGTCATTCCTCTAGGCCAGCAATCGCtgcct                   >  7:1692058/1‑147 (MQ=255)
                                                                                                                           tGGGTATAGCGTCGTGGAC‑AGTAATTGATATTTATGCCCCACCAAAAGCAAAAAACCCCCGAAA‑CCGGTTTTTAAGTAAATAAGGTGAAAATGACCGATAAACCGGGTTATGTCGTGGACAGAAATTAATATAGGCCAGCAATCGCTCAc                    >  7:1603646/1‑150 (MQ=255)
                                                                                                                            gggTATAGCGTCGTTGAC‑ATTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAG‑CTGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACt                   <  1:826654/150‑1 (MQ=255)
                                                                                                                            gggTATAGCGTCGTGGCC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCCGCAATCGCTCACTg                  >  4:595791/1‑151 (MQ=255)
                                                                                                                              gTATAGTGTCGTGGAC‑AGTCATTCATCTTTCTGCGTCTCAAAAAGCATACACTCGACGAAG‑CTGTTTTTTACTTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg                 <  3:418888/150‑1 (MQ=21)
                                                                                                                                ataGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc              >  2:1167384/1‑151 (MQ=255)
                                                                                                                                     tttttttaC‑ATTCATTCATCTTTCTGCCCCTACAATAGTATAAACCCGCTTTAG‑CTTGTTTTTTCTTTAATCATGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc          <  7:783885/143‑1 (MQ=21)
                                                                                                                                      tagtGGAA‑AGTCATTCATCTTTCTGCCCCTAAAAAAGCAAAAACCCGCCGAAG‑CTGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGTGTTCTGTCTTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca         <  4:533145/148‑1 (MQ=21)
                                                                                                                                       cgtGGAC‑AGTCATTCATCTTTCTGACCATCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcagc       >  4:1524883/1‑151 (MQ=255)
                                                                                                                                        gtGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTg                                                          >  4:773101/1‑99 (MQ=2)
                                                                                                                                        gtGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc      >  7:481739/1‑151 (MQ=255)
                                                                                                                                         tGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt     >  2:1916609/1‑151 (MQ=255)
                                                                                                                                         gggAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGAGCTATAAGCCGGCTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt     <  8:1075576/150‑1 (MQ=255)
                                                                                                                                          ggAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCt                                                           <  4:197681/96‑1 (MQ=40)
                                                                                                                                            |                                                                                                                                                        
CGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAC‑AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270011‑3270301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑