Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,4250CT53.2% 128.8 / inf 519P359L (CCA→CTA) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (121/121);  new base T (132/143);  total (253/266)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 529 total aligned reads displayed.

GGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTG  >  NC_000913/4186277‑4186575
                                                                                                                                                    |                                                                                                                                                      
      cgcgGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGc                                                                                                                                                >  3:926953/1‑151 (MQ=255)
      cgcgGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTg                                                                                                                                                 <  3:1316818/150‑1 (MQ=255)
       gcgGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGTTCGTTCTGTAATCACCGTAGGTCCATACCTGCg                                                                                                                                               <  6:918413/151‑1 (MQ=255)
       gcgGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGGCGTTCTGTAATCACCGTAGGTCTATACCTGCg                                                                                                                                               >  1:1397725/1‑151 (MQ=255)
       gcgGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTACGTCAGAACCTGCTCGGTAAGCGTGTTTACTACTACGGTCGTTAAGTAATAACAGTAGGTACATACCTGCg                                                                                                                                               >  7:1606304/1‑151 (MQ=255)
         ggTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAATTCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTc                                                                                                                                             <  5:418975/151‑1 (MQ=255)
         ggTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTc                                                                                                                                             >  5:1382562/1‑151 (MQ=255)
          gtcgtcCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCt                                                                                                                                            <  5:1230603/151‑1 (MQ=255)
          gTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGCTCCATACCTGCGTCt                                                                                                                                            >  5:548619/1‑151 (MQ=255)
          gTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGATGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCt                                                                                                                                            <  1:1098736/151‑1 (MQ=255)
           tCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTg                                                                                                                                           >  5:786566/1‑151 (MQ=255)
            cGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGc                                                                                                                                          <  4:433702/151‑1 (MQ=255)
             gTGCGATCACCGGTTCTAACAAGCGTCCTCTGAACTCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCa                                                                                                                                         <  6:67557/151‑1 (MQ=255)
               gCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATc                                                                                                                                       >  4:1452709/1‑151 (MQ=255)
                cGATCACCGGTTCTAACACGCGTCCTATGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATc                                                                                                                                       <  4:1651362/150‑1 (MQ=255)
                cGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCa                                                                                                                                      <  4:221093/151‑1 (MQ=255)
                cGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCa                                                                                                                                      <  2:1853322/151‑1 (MQ=255)
                 gATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCac                                                                                                                                     <  4:309501/151‑2 (MQ=255)
                   tCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTg                                                                                                                                   >  1:296114/1‑151 (MQ=255)
                    cACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTg                                                                                                                                   <  1:829414/150‑1 (MQ=255)
                    aaCCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGTTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGc                                                                                                                                  <  8:272374/150‑1 (MQ=255)
                       cGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGt                                                                                                                               >  3:1863800/1‑151 (MQ=255)
                        ggTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTc                                                                                                                              <  3:241928/151‑1 (MQ=255)
                           tCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTg                                                                                                                            <  6:1498360/150‑1 (MQ=255)
                            cTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGTTAAGCGTGTTGCCTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGcc                                                                                                                          >  8:1639314/1‑151 (MQ=255)
                              aaCAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGcc                                                                                                                          <  4:656474/149‑1 (MQ=255)
                              aaCAAGAGTCCTCTGAAATCTTTGGGCGACATGATCAAAGGTACAGTGGTTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTTACTACTCGGGTCGTTCTGTAATCACCGTAGGTCTATACGTGCGTCTTCATCAGTGCGGTCTGCCg                                                                                                                         <  5:357680/150‑1 (MQ=255)
                                cAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTTATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGGCgatg                                                                                                                      >  1:1258320/1‑149 (MQ=255)
                                cAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCAACAGTGCGGTATGCCga                                                                                                                        >  8:700085/1‑149 (MQ=255)
                                   gCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGaaa                                                                                                                   <  4:130450/151‑1 (MQ=255)
                                     gTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAAt                                                                                                                 >  6:554765/1‑151 (MQ=255)
                                      tCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATg                                                                                                                >  8:409740/1‑151 (MQ=255)
                                         tctGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGAGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGAGGTCTGCAGAAGAAAATgga                                                                                                              >  3:1066086/1‑149 (MQ=255)
                                          ctGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATAACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCAc                                                                                                            >  6:1917501/1‑151 (MQ=255)
                                           tGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACt                                                                                                           >  8:603601/1‑151 (MQ=255)
                                           tGAAATCTTTGGCCGACATGATCAAAGGTAAAAAGGGTCGTTTCCGTCAGAACCTGCTAGGTAAGCGTTTTGAATAATACGGTCGTTCTGTAATCAACTTAGTTCTATAACTGCGTATGCATAAGTGAGGTCTTCCGAAGaaaaagatcgc                                                                                                           >  8:1051924/1‑144 (MQ=255)
                                            gAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCAc                                                                                                            <  6:778714/149‑1 (MQ=255)
                                              aaTCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGa                                                                                                        <  3:355752/151‑1 (MQ=255)
                                              aaTCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTAATCCGGTCGTTGTTTAAACACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTTCCGAACAAAATGGCACTggg                                                                                                        >  8:807864/1‑150 (MQ=255)
                                               aTCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTgg                                                                                                         >  1:529356/1‑149 (MQ=255)
                                                         gACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAAc                                                                                             >  7:261001/1‑151 (MQ=255)
                                                            aTGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGt                                                                                          >  2:774323/1‑151 (MQ=255)
                                                              gATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGttg                                                                                        >  3:480285/1‑150 (MQ=255)
                                                               aTCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTc                                                                                        >  7:124593/1‑150 (MQ=255)
                                                               aTCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCa                                                                                       <  7:928346/151‑1 (MQ=255)
                                                                  aaaGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCCTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATCGCACTGGAGCTGTTCAAACCGTTCATCt                                                                                    >  7:396218/1‑151 (MQ=255)
                                                                  aaaGGTAAACAGGGGCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCt                                                                                    <  7:601136/151‑1 (MQ=255)
                                                                    aGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGNTCAAACCGTNNATCNNc                                                                                  >  4:576819/1‑151 (MQ=255)
                                                                         aaCAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCaa                                                                             <  1:1554946/151‑1 (MQ=255)
                                                                         aaCAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCaa                                                                             <  6:1777823/151‑1 (MQ=255)
                                                                           cAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGc                                                                           >  7:1862602/1‑151 (MQ=255)
                                                                            aGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCt                                                                          >  3:1541829/1‑151 (MQ=255)
                                                                                    ttCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGc                                                                  >  6:1232609/1‑151 (MQ=255)
                                                                                        gTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTgg                                                              >  4:940272/1‑151 (MQ=255)
                                                                                        gTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTgg                                                              >  1:1893311/1‑151 (MQ=255)
                                                                                        gTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTgg                                                              <  7:1413743/151‑1 (MQ=255)
                                                                                          cAGAACCTGCTCGGGAAGCGTGGTGACTACTCCGGTCGTTCTGTCATCTGCGTAGGTCGATACCTGCGTCTGCTTCAGTGCGGGCTGCGGAAGAAAATGGCACTGGAGCTGTTCATACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTc                                                            <  7:71399/151‑1 (MQ=255)
                                                                                           aGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGCACTGCGTGGTCt                                                           >  1:1789675/1‑151 (MQ=255)
                                                                                                cTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCt                                                       >  3:1471638/1‑150 (MQ=255)
                                                                                                 tGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTac                                                     >  8:140279/1‑151 (MQ=255)
                                                                                                     cGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTaccacc                                                 >  2:1076721/1‑151 (MQ=255)
                                                                                                     cGGTAAGCGGGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTCGTCTTGCTaccacc                                                 >  7:1732197/1‑151 (MQ=255)
                                                                                                      ggTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTaccacc                                                 >  2:1742213/1‑150 (MQ=255)
                                                                                                      ggTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCCGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTaccacc                                                 >  5:288298/1‑150 (MQ=255)
                                                                                                          aGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTa                                             >  3:1206553/1‑150 (MQ=255)
                                                                                                           gCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTaaa                                           <  2:628206/151‑1 (MQ=255)
                                                                                                           gCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTaaa                                           <  4:109043/151‑1 (MQ=255)
                                                                                                           gCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTaaa                                           <  7:502276/151‑1 (MQ=255)
                                                                                                              ggtTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCt                                        <  2:1201173/150‑1 (MQ=255)
                                                                                                               gtTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTg                                       <  7:795831/151‑1 (MQ=255)
                                                                                                                   actactCCGGTCGTTCTGTAATCACCGTAGGTCTTTACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGGTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCgaa                                   <  2:422997/151‑1 (MQ=255)
                                                                                                                      actCCGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCgaaga                                 >  5:107464/1‑150 (MQ=255)
                                                                                                                        tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGaaa                               >  3:1599158/1‑150 (MQ=255)
                                                                                                                         ccGGTCGTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGaaa                               <  4:1200805/149‑1 (MQ=255)
                                                                                                                               gTTCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGa                       <  5:923561/151‑1 (MQ=255)
                                                                                                                                ttCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTg                        >  2:223707/1‑149 (MQ=255)
                                                                                                                                 tCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAgc                     >  8:185748/1‑151 (MQ=255)
                                                                                                                                 tCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAgc                     >  3:520422/1‑151 (MQ=255)
                                                                                                                                 tCTGTAATCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAg                      <  8:1488636/150‑1 (MQ=255)
                                                                                                                                 tCTGTAATCACCGTAGGTCCATACCTGCGGCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAg                      <  5:1796575/150‑1 (MQ=255)
                                                                                                                                    gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAgcgc                   <  6:982835/150‑1 (MQ=255)
                                                                                                                                    gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGcgcg                  <  3:640601/151‑1 (MQ=255)
                                                                                                                                      aaTCACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaa                <  5:1581437/151‑1 (MQ=255)
                                                                                                                                      aaTCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaa                <  6:1395235/151‑1 (MQ=255)
                                                                                                                                         cACCGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaaga              <  3:1707905/150‑1 (MQ=255)
                                                                                                                                         cACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaagaa             <  1:1646686/151‑1 (MQ=255)
                                                                                                                                         cACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaaga              <  4:984226/150‑1 (MQ=255)
                                                                                                                                         cACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaaga              <  2:1606441/150‑1 (MQ=255)
                                                                                                                                           ccGTAGGTCCATACCTGCGTCTTCATCAGGGCGGTCTGCCGAAGAAAATGGCACTGGAGATGTTCAAACCGTTAATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc           <  4:1841066/151‑1 (MQ=255)
                                                                                                                                           ccGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc           >  8:1231877/1‑151 (MQ=255)
                                                                                                                                            cGTAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt          >  1:658841/1‑151 (MQ=255)
                                                                                                                                            cGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt          <  2:1475867/151‑1 (MQ=255)
                                                                                                                                              tAGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGt        >  5:1913605/1‑151 (MQ=255)
                                                                                                                                              tAGGTCCATACCTGCTTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGt        >  2:1377188/1‑151 (MQ=255)
                                                                                                                                              tAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTgg        >  3:847763/1‑150 (MQ=255)
                                                                                                                                               aGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTc       >  3:601214/1‑151 (MQ=255)
                                                                                                                                               aGGTCTATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTc       <  1:959493/151‑1 (MQ=255)
                                                                                                                                                 gTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt     >  2:858768/1‑151 (MQ=255)
                                                                                                                                                   ccATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGtt    <  1:467733/150‑1 (MQ=255)
                                                                                                                                                    cATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg  <  6:326771/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTG  >  NC_000913/4186277‑4186575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑