Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,7670G.31.6% 646.9 / 310.3 244coding (855/1035 nt)tqsApheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base G (90/77);  new base . (38/39);  total (128/116)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 248 total aligned reads displayed.

CATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGC  >  NC_000913/1674617‑1674916
                                                                                                                                                      |                                                                                                                                                     
cATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATgggg                                                                                                                                                       >  5:1649117/1‑151 (MQ=255)
  tCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCg                                                                                                                                                     >  4:327923/1‑151 (MQ=255)
  tCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGCCAATATTCTGGAGCCGCGTATCATGGGGCg                                                                                                                                                     >  8:194545/1‑151 (MQ=255)
   cccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctt                                                                                                                                                    >  4:503700/1‑149 (MQ=255)
   cccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGct                                                                                                                                                     <  8:1232814/150‑2 (MQ=255)
    ccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTg                                                                                                                                                   >  3:1165131/1‑151 (MQ=255)
    ccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCCTATCATGGGGCGTg                                                                                                                                                   >  7:73500/1‑151 (MQ=255)
       aaTATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctttcca                                                                                                                                                <  6:1269321/151‑7 (MQ=255)
       aaTATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGCTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATGTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctttcc                                                                                                                                                 >  5:403686/1‑145 (MQ=255)
          atTTGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTgg                                                                                                                                             <  2:353934/151‑1 (MQ=255)
            tGGTTCAGTCCTCGCGGCAGTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTgggg                                                                                                                                           >  3:815896/1‑151 (MQ=255)
            tGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctttccacatt                                                                                                                                            <  2:1468716/150‑11 (MQ=255)
              gTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGctttccacattggt                                                                                                                                         <  2:794059/151‑14 (MQ=255)
                  aGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTcc                                                                                                                                     >  1:595722/1‑151 (MQ=255)
                       gcgcgGCAATCCCCGCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGTATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTTGGCTGGGGCTTTCCACAtt                                                                                                                                <  2:318455/150‑1 (MQ=255)
                        cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACAt                                                                                                                                 <  2:1358980/149‑1 (MQ=255)
                        cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                               <  3:772316/151‑1 (MQ=255)
                         gcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtgg                                                                                                                              <  7:81394/151‑1 (MQ=255)
                           ggCAATCCCCCCTCTCGCTCAGGTACTGGTGTTTAATGGCTTCTAGGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtggtg                                                                                                                            <  1:194424/151‑1 (MQ=255)
                            gCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtggtgg                                                                                                                           >  4:1697500/1‑151 (MQ=255)
                             cAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTggtggt                                                                                                                          >  7:1670023/1‑151 (MQ=255)
                              aaTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTCGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCAGATTggtggt                                                                                                                          >  2:1077989/1‑150 (MQ=255)
                               aTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTAt                                                                                                                        >  6:1614007/1‑151 (MQ=255)
                               aTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTAt                                                                                                                        <  1:722041/151‑1 (MQ=255)
                                 ccccccTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTAttt                                                                                                                      <  7:1425432/151‑1 (MQ=255)
                                        aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTc                                                                                                                 <  7:225989/149‑1 (MQ=255)
                                        aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGt                                                                                                               <  6:368181/151‑1 (MQ=255)
                                        aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg                                                                                                    <  4:843932/150‑1 (MQ=255)
                                           gCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGATCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTgt                                                                                                            >  1:948178/1‑150 (MQ=255)
                                             tCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAtt                                                                                                          >  4:966488/1‑151 (MQ=255)
                                              cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATgg                                                                                              <  2:623247/150‑1 (MQ=255)
                                              cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgt                                                                                             >  5:1240902/1‑151 (MQ=255)
                                              cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgt                                                                                             <  1:1469896/151‑1 (MQ=255)
                                               aGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCTGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGGATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTCGTATTTTTGTCGTTGATTTTTTGGGGAAGgtt                                                                                            >  3:154289/1‑151 (MQ=255)
                                                   aCTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGGTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg                                                                                                    <  8:609877/151‑1 (MQ=255)
                                                   aCTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgttt                                                                                        >  7:1196732/1‑150 (MQ=255)
                                                         gtTTAATGGCTTCTACGAAGGGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGGCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                               <  5:771307/150‑1 (MQ=255)
                                                         gtTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                                   <  1:1154657/150‑1 (MQ=255)
                                                            tAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTg                                                                               >  5:687144/1‑151 (MQ=255)
                                                             aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttg                                                                                           <  7:598149/150‑1 (MQ=255)
                                                               tGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGt                                                                            >  5:1047301/1‑151 (MQ=255)
                                                                 gCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAt                                                                          >  5:1309854/1‑151 (MQ=255)
                                                                 gCTTCTACGAAGCGTTGCTGGTGCGGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                      <  8:729658/151‑1 (MQ=255)
                                                                   ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAggcc                                                                                    >  7:382168/1‑149 (MQ=255)
                                                                   ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAc                                                                                    >  3:1476594/1‑151 (MQ=255)
                                                                    tCTACGAAGCGTTGCTTCTGCTCGCGGCATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGACGCGAATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGCTGGTATTTTTGTCGTTGATTTTTTGGTGAAGGTTGTTAGGACAGGTGGGAAtgct                                                                       >  6:541/1‑151 (MQ=255)
                                                                    tCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                                   >  4:162567/1‑151 (MQ=255)
                                                                    tCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGGATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgcg                                                                       >  1:1584460/1‑150 (MQ=255)
                                                                     cTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctg                                                                      >  3:1719207/1‑151 (MQ=255)
                                                                      tACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCgg                                                                                 >  3:1004933/1‑151 (MQ=255)
                                                                       aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctgc                                                                     <  5:1403073/150‑1 (MQ=255)
                                                                       aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgct                                                                       <  6:1021976/148‑1 (MQ=255)
                                                                       aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATgctgct                                                                    >  2:1648258/1‑151 (MQ=255)
                                                                       aCGAACCGTTCCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCCGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTCGAGGTATTTTTGTCGGTGATTTTTTGGGGATGGTTGTTAGGACCGGt                                                                                >  4:1016922/1‑151 (MQ=255)
                                                                        ggAAGCGTTGCTGGTGCTGGCGGGATATGTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTg                                                                               <  7:498067/150‑1 (MQ=255)
                                                                        cGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCtt                                                                   <  2:519916/151‑1 (MQ=255)
                                                                        cGAAGCGTTGCTGGTGCTGGCGGGATATCTGATGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTTGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTg                                                                               <  3:1051672/151‑1 (MQ=255)
                                                                         gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                              >  6:1459468/1‑151 (MQ=255)
                                                                          aaGCGTTGCTGGTGCTGGAGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTc                                                                 <  3:722153/151‑1 (MQ=255)
                                                                          aaGCCTTGCTGGTGCTGCCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTCGGCAGCGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTAGTTAGGACCGGTggg                                                                             >  1:411425/1‑151 (MQ=255)
                                                                           aGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGt                                                                            <  7:232643/151‑1 (MQ=255)
                                                                           aGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGt                                                                            <  4:741064/151‑1 (MQ=255)
                                                                           aGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTcc                                                                <  7:664024/151‑1 (MQ=255)
                                                                            gCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTa                                                                           <  2:1292742/151‑1 (MQ=255)
                                                                            gCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCg                                                               <  6:1238939/151‑1 (MQ=255)
                                                                             cGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                              <  5:1471677/151‑1 (MQ=255)
                                                                                  ctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                        >  1:1092539/1‑151 (MQ=255)
                                                                                   tggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTTTGCTGCTTTCCGTGCCGtt                                                        >  4:49680/1‑151 (MQ=255)
                                                                                    ggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGt                                                         <  6:1705281/149‑1 (MQ=255)
                                                                                    ggtgctggCGGGATATCTGCTGATTAATCTGGGCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                       <  6:1554021/151‑1 (MQ=255)
                                                                                     gtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGAGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTTTGACCGGTGGGTATGCTGCttt                                                                  >  1:296807/1‑151 (MQ=255)
                                                                                     gtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCAGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCttt                                                                  <  2:1166892/151‑1 (MQ=255)
                                                                                      tgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAGCGGTGGGTATGCTGCTTTCCGTGCCGTTGAc                                                     >  6:1121860/1‑151 (MQ=255)
                                                                                         tggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGACGTTGACAat                                                  >  3:1686217/1‑151 (MQ=255)
                                                                                           gCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatta                                                <  2:223370/151‑1 (MQ=255)
                                                                                              ggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTg                                             <  8:1038179/151‑1 (MQ=255)
                                                                                                 tatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                       >  8:1108431/1‑150 (MQ=255)
                                                                                                  atCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                      >  3:913181/1‑150 (MQ=255)
                                                                                                  atCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCa                                          <  7:1645355/150‑1 (MQ=255)
                                                                                                      gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                 >  7:1067812/1‑151 (MQ=255)
                                                                                                      gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                                  <  6:201328/150‑1 (MQ=255)
                                                                                                      gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                     <  3:576123/151‑1 (MQ=255)
                                                                                                         gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                  >  8:608610/1‑151 (MQ=255)
                                                                                                              aTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGtga                                          >  5:481319/1‑148 (MQ=255)
                                                                                                               tCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGATGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaa                                         >  6:1391251/1‑150 (MQ=255)
                                                                                                                 tGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                      >  1:1125681/1‑151 (MQ=255)
                                                                                                                  ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                     <  4:1173729/151‑1 (MQ=255)
                                                                                                                       tCGGGAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCGGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                  <  5:1578922/149‑1 (MQ=255)
                                                                                                                         ggCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTg                              <  8:834081/151‑1 (MQ=255)
                                                                                                                            aaTATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaac                           >  2:1062981/1‑151 (MQ=255)
                                                                                                                               atTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaa                         >  1:686342/1‑150 (MQ=255)
                                                                                                                                ttCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                       >  8:1482876/1‑151 (MQ=255)
                                                                                                                                 tCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                       >  1:848110/1‑150 (MQ=255)
                                                                                                                                 tCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                       <  2:435894/150‑1 (MQ=255)
                                                                                                                                    ggAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCg                   >  5:799294/1‑151 (MQ=255)
                                                                                                                                        ccGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCggg                 >  8:237354/1‑149 (MQ=255)
                                                                                                                                        ccGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCggg                 >  5:1727412/1‑149 (MQ=255)
                                                                                                                                         cgcgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAgg              >  5:587360/1‑151 (MQ=255)
                                                                                                                                               tCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCaa          >  7:1273820/1‑149 (MQ=255)
                                                                                                                                                cATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc       >  6:845543/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGC  >  NC_000913/1674617‑1674916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: