Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,7690G.33.1% 591.9 / 312.1 233coding (857/1035 nt)tqsApheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base G (85/71);  new base . (38/39);  total (123/110)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-02

Only 100 of 235 total aligned reads displayed.

TCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCG  >  NC_000913/1674619‑1674918
                                                                                                                                                      |                                                                                                                                                     
tCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCg                                                                                                                                                       >  4:327923/1‑151 (MQ=255)
 cccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGt                                                                                                                                                      <  6:94689/151‑1 (MQ=255)
  ccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTg                                                                                                                                                     >  2:538411/1‑151 (MQ=255)
      atatTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCt                                                                                                                                                 >  2:1087197/1‑151 (MQ=255)
        atTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTgg                                                                                                                                               <  2:1019008/151‑1 (MQ=255)
                    cTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACAt                                                                                                                                   >  3:1203405/1‑151 (MQ=255)
                      cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACAt                                                                                                                                   <  5:1215196/149‑1 (MQ=255)
                      cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                                 <  3:772316/151‑1 (MQ=255)
                       gcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtgg                                                                                                                                <  7:81394/151‑1 (MQ=255)
                          gCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtggtgg                                                                                                                             >  4:1697500/1‑151 (MQ=255)
                           cAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTggtggt                                                                                                                            >  7:1175623/1‑151 (MQ=255)
                           cAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTggtggt                                                                                                                            >  7:1670023/1‑151 (MQ=255)
                                cccccTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTAtttt                                                                                                                       >  6:1570175/1‑151 (MQ=255)
                                     tATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg                                                                                                      >  1:876640/1‑151 (MQ=255)
                                      aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTggg                                                                                                     >  6:761083/1‑151 (MQ=255)
                                      aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg                                                                                                      <  4:843932/150‑1 (MQ=255)
                                         gCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGAt                                                                                                  <  6:1721587/151‑1 (MQ=255)
                                           tCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAtt                                                                                                            >  4:966488/1‑151 (MQ=255)
                                            cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgt                                                                                               >  3:731806/1‑151 (MQ=255)
                                            cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgt                                                                                               <  1:1469896/151‑1 (MQ=255)
                                             aGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCTGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGGATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTCGTATTTTTGTCGTTGATTTTTTGGGGAAGgtt                                                                                              >  3:154289/1‑151 (MQ=255)
                                               gTACTGGTGTTTAATGGCTTCTACGATGCTTTGCTTGTGCTGGCTGTATATCTTCTGATTAATCTGGTCTTAGTCAATATTCTGGAGCCGCGTATCATTTGGCGTGGGCTGTTGCTTTCCACATTGGTTTTATTTTTGTCGTTGAtttttt                                                                                                        <  1:428067/151‑1 (MQ=255)
                                                tACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                           >  7:1305815/1‑151 (MQ=255)
                                                tACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                           >  8:1568100/1‑151 (MQ=255)
                                                 aCTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGGTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg                                                                                                      <  8:609877/151‑1 (MQ=255)
                                                 aCTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg                                                                                                      <  5:496039/151‑1 (MQ=255)
                                                     gtgtTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGATCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGAt                                                                                                  <  5:44829/151‑1 (MQ=255)
                                                       gtTTAATGGCTTCTACGAAGGGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGGCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                 <  5:771307/150‑1 (MQ=255)
                                                       gtTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                                     <  1:1154657/150‑1 (MQ=255)
                                                        tttAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATGTTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgt                                                                                               >  4:110533/1‑151 (MQ=255)
                                                           aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgt                                                                                            >  2:339628/1‑151 (MQ=255)
                                                           aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                <  6:126687/151‑1 (MQ=255)
                                                             tGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGt                                                                              >  5:1047301/1‑151 (MQ=255)
                                                               gCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                        <  7:1228222/151‑1 (MQ=255)
                                                               gCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAt                                                                            >  5:1309854/1‑151 (MQ=255)
                                                               gCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAt                                                                            <  6:1646143/151‑1 (MQ=255)
                                                               gCTTCTACGAAGCGTTGCTGGTGCGGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                        <  8:729658/151‑1 (MQ=255)
                                                                 ttatACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgg                                                                          >  1:688534/4‑150 (MQ=255)
                                                                 ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAggcc                                                                                      >  2:445853/1‑149 (MQ=255)
                                                                 ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAc                                                                                      >  3:1476594/1‑151 (MQ=255)
                                                                 ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAGCGGTGGGTAtgc                                                                          >  1:60292/1‑151 (MQ=255)
                                                                  tCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                                     >  4:162567/1‑151 (MQ=255)
                                                                  tCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGGATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgcg                                                                         >  1:1584460/1‑150 (MQ=255)
                                                                   cTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctg                                                                        >  3:1719207/1‑151 (MQ=255)
                                                                     aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctgc                                                                       <  5:1403073/150‑1 (MQ=255)
                                                                     aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgct                                                                         <  6:1021976/148‑1 (MQ=255)
                                                                     aCGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATgctgct                                                                      <  7:5768/151‑1 (MQ=255)
                                                                      cGAAGCGTTGCTGGTGCTGGCGGGATATCTGATGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTTGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTg                                                                                 <  3:1051672/151‑1 (MQ=255)
                                                                       gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                <  6:1340875/151‑1 (MQ=255)
                                                                        aaGCGTTGCTGGTGCTGGAGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTc                                                                   <  3:722153/151‑1 (MQ=255)
                                                                         aGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGt                                                                              <  4:741064/151‑1 (MQ=255)
                                                                         aGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGt                                                                              <  7:232643/151‑1 (MQ=255)
                                                                           cGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAt                                                                            <  5:1461738/151‑1 (MQ=255)
                                                                           cGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                                <  4:343126/151‑1 (MQ=255)
                                                                           cGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                                <  5:1603479/151‑1 (MQ=255)
                                                                           cGTTGCTGGTGCGGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAt                                                                            <  1:38076/151‑1 (MQ=255)
                                                                            gTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtg                                                                           >  7:642651/1‑151 (MQ=255)
                                                                             ttGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGTGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGc                                                              >  3:1016137/1‑151 (MQ=255)
                                                                              tgctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgct                                                                         >  2:296745/1‑151 (MQ=255)
                                                                                ctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGA‑TTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                          >  1:1092539/1‑151 (MQ=255)
                                                                                 tggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                          >  2:1461637/1‑151 (MQ=255)
                                                                                  ggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCtt                                                                     >  2:1283362/1‑151 (MQ=255)
                                                                                  ggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                         <  2:405863/151‑1 (MQ=255)
                                                                                   gtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGAGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTTTGACCGGTGGGTATGCTGCttt                                                                    >  1:296807/1‑151 (MQ=255)
                                                                                   gtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                        <  5:660571/151‑1 (MQ=255)
                                                                                    tgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAGCGGTGGGTATGCTGCTTTCCGTGCCGTTGAc                                                       >  6:1121860/1‑151 (MQ=255)
                                                                                       tggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                                >  1:1705533/1‑151 (MQ=255)
                                                                                       tggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGACGTTGACAat                                                    >  3:1686217/1‑151 (MQ=255)
                                                                                        ggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTg                                                               <  6:251364/151‑1 (MQ=255)
                                                                                        ggCGGGATATCTGCTGATTAATCTGGTCTTCCGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                   <  6:1402732/151‑1 (MQ=255)
                                                                                         gCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGc                                                              <  3:537651/151‑1 (MQ=255)
                                                                                         gCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatta                                                  <  2:223370/151‑1 (MQ=255)
                                                                                            ggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGt                                                           >  7:302812/1‑151 (MQ=255)
                                                                                            ggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGt                                                           >  2:854395/1‑151 (MQ=255)
                                                                                               tatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                         >  8:1108431/1‑150 (MQ=255)
                                                                                               tatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                             >  2:1576165/1‑150 (MQ=255)
                                                                                                atCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                        >  3:913181/1‑150 (MQ=255)
                                                                                                atCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCa                                            <  7:1645355/150‑1 (MQ=255)
                                                                                                  ctgctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                          >  8:214247/1‑150 (MQ=255)
                                                                                                    gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                   >  1:746353/1‑151 (MQ=255)
                                                                                                    gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                          <  2:1317167/148‑1 (MQ=255)
                                                                                                    gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                       <  8:1423802/151‑1 (MQ=255)
                                                                                                     ctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                   >  7:482176/1‑150 (MQ=255)
                                                                                                       gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                    >  8:608610/1‑151 (MQ=255)
                                                                                                        aTTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcgc                                   >  5:715677/1‑151 (MQ=255)
                                                                                                          tAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCtt                                 >  3:862924/1‑151 (MQ=255)
                                                                                                          tAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCt                                  <  5:898599/150‑1 (MQ=255)
                                                                                                           aaTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                             <  7:97488/150‑1 (MQ=255)
                                                                                                               tGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                        >  1:1125681/1‑151 (MQ=255)
                                                                                                                ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                       <  4:1173729/151‑1 (MQ=255)
                                                                                                                     tCGGGAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCGGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                    <  5:1578922/149‑1 (MQ=255)
                                                                                                                       ggCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTg                                <  8:834081/151‑1 (MQ=255)
                                                                                                                          aaTATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaac                             >  2:1062981/1‑151 (MQ=255)
                                                                                                                                      ccGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCggg                   >  5:1727412/1‑149 (MQ=255)
                                                                                                                                         cgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTc              >  7:192911/1‑151 (MQ=255)
                                                                                                                                            aTCATGGGGCGTGGGCTGGGGCGTTCCACATTGGTGGTATTTTTGTCGGTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCaaa           <  6:53881/151‑1 (MQ=255)
                                                                                                                                             tCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTAAAAATTGCGCTTGAACAAAAAGCGGGAGGTCaaac          >  8:536040/1‑150 (MQ=255)
                                                                                                                                              cATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTGCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACACACCGCCGGAGGGCAAAGc         >  6:1530350/1‑151 (MQ=255)
                                                                                                                                                   ggCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGc    >  7:687044/1‑151 (MQ=255)
                                                                                                                                                     cGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCg  >  4:505926/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCG  >  NC_000913/1674619‑1674918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑