Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,7710G.34.1% 570.6 / 313.0 228coding (859/1035 nt)tqsApheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base G (81/68);  new base . (38/39);  total (120/108)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

Only 100 of 233 total aligned reads displayed.

CCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTT  >  NC_000913/1674621‑1674920
                                                                                                                                                      |                                                                                                                                                     
ccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTg                                                                                                                                                       >  3:1165131/1‑151 (MQ=255)
ccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTg                                                                                                                                                       <  4:329550/151‑1 (MQ=255)
ccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTg                                                                                                                                                       >  2:538411/1‑151 (MQ=255)
ccGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCCTATCATGGGGCGTg                                                                                                                                                       >  7:73500/1‑151 (MQ=255)
    atatTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATTTTCTGGAGCCGCGTATCATGGGGCGTGGGCt                                                                                                                                                   >  5:1673675/1‑151 (MQ=255)
      atTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTgg                                                                                                                                                 <  2:1019008/151‑1 (MQ=255)
        tGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTggg                                                                                                                                                <  5:798064/150‑1 (MQ=255)
        tGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTggcg                                                                                                                                               >  3:510159/1‑149 (MQ=255)
         ggTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTgggg                                                                                                                                               <  4:762203/150‑1 (MQ=255)
               gTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCca                                                                                                                                        <  2:282252/151‑1 (MQ=255)
                    cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACAt                                                                                                                                     <  2:1358980/149‑1 (MQ=255)
                    cgcgGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                                   <  5:1592889/151‑1 (MQ=255)
                     gcgTCAATCCCGCCTATCGGTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtg                                                                                                                                   <  1:600964/150‑1 (MQ=255)
                       ggCAATCCCCCCTATCGCTGAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtggtg                                                                                                                                <  8:1140878/151‑1 (MQ=255)
                        gCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATtggtgg                                                                                                                               >  4:1697500/1‑151 (MQ=255)
                         cAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTggtggt                                                                                                                              >  7:1175623/1‑151 (MQ=255)
                          aaTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTCGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCAGATTggtggt                                                                                                                              >  2:1077989/1‑150 (MQ=255)
                           aTCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTAt                                                                                                                            >  6:1614007/1‑151 (MQ=255)
                                    aTCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGt                                                                                                                   <  6:368181/151‑1 (MQ=255)
                                        gtCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAt                                                                                                               <  1:1331642/150‑1 (MQ=255)
                                          cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAttt                                                                                                             <  5:28521/151‑1 (MQ=255)
                                          cAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATgg                                                                                                  <  2:623247/150‑1 (MQ=255)
                                           aGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCTGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGGATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTCGTATTTTTGTCGTTGATTTTTTGGGGAAGgtt                                                                                                >  3:154289/1‑151 (MQ=255)
                                             gTACTGGTGTTTAATGGCTTCTACGATGCTTTGCTTGTGCTGGCTGTATATCTTCTGATTAATCTGGTCTTAGTCAATATTCTGGAGCCGCGTATCATTTGGCGTGGGCTGTTGCTTTCCACATTGGTTTTATTTTTGTCGTTGAtttttt                                                                                                          <  1:428067/151‑1 (MQ=255)
                                              tACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                             >  7:1305815/1‑151 (MQ=255)
                                              tACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                             >  4:1628001/1‑151 (MQ=255)
                                                  ggTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATGAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgggg                                                                                                      >  3:972254/1‑150 (MQ=255)
                                                    tgtTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                   >  2:1677198/1‑151 (MQ=255)
                                                     gtTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                                       <  1:1154657/150‑1 (MQ=255)
                                                      tttAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATGTTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgt                                                                                                 >  4:110533/1‑151 (MQ=255)
                                                        tAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTg                                                                                   >  5:687144/1‑151 (MQ=255)
                                                         aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgt                                                                                              >  2:339628/1‑151 (MQ=255)
                                                         aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttg                                                                                               <  7:598149/150‑1 (MQ=255)
                                                         aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                  <  7:234911/151‑1 (MQ=255)
                                                         aaTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                  <  7:226956/151‑1 (MQ=255)
                                                          aTGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                             <  2:392012/151‑1 (MQ=255)
                                                            ggCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                             >  6:1041602/1‑149 (MQ=255)
                                                             gCTTCTACGAAGCGTTGCTGGTGCGGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAgg                                                                                          <  8:729658/151‑1 (MQ=255)
                                                               ttatACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgg                                                                            >  1:688534/4‑150 (MQ=255)
                                                               ttCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAc                                                                                        >  5:1328533/1‑151 (MQ=255)
                                                                tCTACGAAGCGTTGCTTCTGCTCGCGGCATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGACGCGAATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGCTGGTATTTTTGTCGTTGATTTTTTGGTGAAGGTTGTTAGGACAGGTGGGAAtgct                                                                           >  6:541/1‑151 (MQ=255)
                                                                tCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGGATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgcg                                                                           >  1:1584460/1‑150 (MQ=255)
                                                                  tACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCgg                                                                                     >  3:1004933/1‑151 (MQ=255)
                                                                    cGAAGCGTTGCTGGTGCTGGCGGGATATCTGATGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTTGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTg                                                                                   <  3:1051672/151‑1 (MQ=255)
                                                                     gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTgg                                                                                  >  6:1459468/1‑151 (MQ=255)
                                                                      aaGCGTTGCTGGTGCTGGAGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTc                                                                     <  3:722153/151‑1 (MQ=255)
                                                                       aGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTcc                                                                    <  4:702189/151‑1 (MQ=255)
                                                                        gCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTGGGCAATATTGTGGAGCCGCGTTTGATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTa                                                                               <  7:848731/151‑1 (MQ=255)
                                                                        gCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGt                                                                                >  3:1446734/1‑150 (MQ=255)
                                                                         cGTTGCTGGTGCGGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAt                                                                              <  1:38076/151‑1 (MQ=255)
                                                                             gctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgct                                                                           <  5:1139907/150‑1 (MQ=255)
                                                                              ctggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                            >  1:1092539/1‑151 (MQ=255)
                                                                                ggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCtt                                                                       >  2:1283362/1‑151 (MQ=255)
                                                                                ggtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                           <  6:1646458/151‑1 (MQ=255)
                                                                                ggtgctggCGGGATATCTGCTGATTAATCTGGGCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                           <  6:1554021/151‑1 (MQ=255)
                                                                                 gtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                          <  8:616715/151‑1 (MQ=255)
                                                                                   gctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTcc                                                                    >  7:1210927/1‑151 (MQ=255)
                                                                                     tggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                                  >  1:1705533/1‑151 (MQ=255)
                                                                                     tggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                                      >  5:578925/1‑151 (MQ=255)
                                                                                     gggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGGGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGt                                                                  <  1:26925/150‑1 (MQ=255)
                                                                                      ggCGGGATATCTGCTGATTAATCTGGGCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACaa                                                       <  7:509209/149‑1 (MQ=255)
                                                                                         gggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCg                                                              >  4:959067/1‑151 (MQ=255)
                                                                                            atatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                           <  5:1572683/151‑1 (MQ=255)
                                                                                             tatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTg                                                           >  8:1108431/1‑150 (MQ=255)
                                                                                             tatCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                               >  2:1576165/1‑150 (MQ=255)
                                                                                                  gctgATTAATCTGGTCTTCGGCAATATTCTGGAGGCGCGTATCATGGGGCGTGGGCTTGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGAc                                                         <  7:1506949/147‑1 (MQ=255)
                                                                                                  gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACaa                                                       <  6:1293780/149‑1 (MQ=255)
                                                                                                  gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                     >  1:746353/1‑151 (MQ=255)
                                                                                                  gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAat                                                      <  6:201328/150‑1 (MQ=255)
                                                                                                  gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaaa                                            <  2:1317167/148‑1 (MQ=255)
                                                                                                  gctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                         <  3:576123/151‑1 (MQ=255)
                                                                                                   ctgATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatt                                                     >  7:482176/1‑150 (MQ=255)
                                                                                                     gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                      >  8:608610/1‑151 (MQ=255)
                                                                                                     gATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                      >  3:793123/1‑151 (MQ=255)
                                                                                                       ttAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGt                                                >  3:746170/1‑151 (MQ=255)
                                                                                                        tAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCA‑‑‑‑‑‑‑‑‑‑‑‑TGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCtt                                   >  3:862924/1‑151 (MQ=255)
                                                                                                          aTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGtga                                              >  5:481319/1‑148 (MQ=255)
                                                                                                           tCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGATGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaa                                             >  6:1391251/1‑150 (MQ=255)
                                                                                                             tGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                          >  1:1125681/1‑151 (MQ=255)
                                                                                                              ggTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                         <  4:1173729/151‑1 (MQ=255)
                                                                                                               gTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTg                                        <  5:1439532/151‑1 (MQ=255)
                                                                                                                    cGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGCCAATTATTGTCAAAATTGCGCtg                                   >  3:1713673/1‑150 (MQ=255)
                                                                                                                     ggCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTg                                  <  8:834081/151‑1 (MQ=255)
                                                                                                                       cAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGa                                 >  5:976676/1‑150 (MQ=255)
                                                                                                                        aaTATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaac                               >  2:1062981/1‑151 (MQ=255)
                                                                                                                         atatTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTCTTAGGACCGCTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaaa                               >  6:114386/1‑149 (MQ=255)
                                                                                                                            ttCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                           >  8:1482876/1‑151 (MQ=255)
                                                                                                                             tCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                           >  1:848110/1‑150 (MQ=255)
                                                                                                                             tCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                           <  2:435894/150‑1 (MQ=255)
                                                                                                                                ggAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCg                       >  7:1666587/1‑151 (MQ=255)
                                                                                                                                  aGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAATTTGCTCTTGAACAAACAGCggg                     >  3:168483/1‑151 (MQ=255)
                                                                                                                                    ccGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCggg                     >  8:237354/1‑149 (MQ=255)
                                                                                                                                       cgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTc                >  5:1014755/1‑151 (MQ=255)
                                                                                                                                       cgTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTc                >  7:192911/1‑151 (MQ=255)
                                                                                                                                         tATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCa               >  5:1260773/1‑150 (MQ=255)
                                                                                                                                           tCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTAAAAATTGCGCTTGAACAAAAAGCGGGAGGTCaaac            >  8:536040/1‑150 (MQ=255)
                                                                                                                                            cATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAg            <  5:768134/150‑1 (MQ=255)
                                                                                                                                            cATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGc           >  6:845543/1‑151 (MQ=255)
                                                                                                                                               ggggCGTGGTCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATc        <  4:500775/151‑1 (MQ=255)
                                                                                                                                                 ggCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGc      >  7:687044/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTT  >  NC_000913/1674621‑1674920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: