Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,610,1070GA45.8% 136.9 / inf 417E541K (GAG→AAG) hyfGhydrogenase 4, subunit
Reads supporting (aligned to +/- strand):  ref base G (94/131);  new base A (75/115);  total (170/247)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-02

Only 100 of 428 total aligned reads displayed.

GGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAA  >  NC_000913/2609961‑2610257
                                                                                                                                                  |                                                                                                                                                      
ggCCGGTGTTGCGTTAGATGCTGCGCGGCAATAGCGTGTCTGACGCACCGCTGATTATCGGTATCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGAt                                                                                                                                                    <  8:314705/151‑1 (MQ=255)
ggCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAaaag                                                                                                                                                      <  3:818724/149‑1 (MQ=255)
ggCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAgaga                                                                                                                                                     <  3:1270999/150‑1 (MQ=255)
ggCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAgaga                                                                                                                                                     <  2:551638/150‑1 (MQ=255)
 gCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATc                                                                                                                                                   <  8:1090775/151‑1 (MQ=255)
  ccGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAag                                                                                                                                                        >  4:1055498/1‑145 (MQ=255)
  ccGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCg                                                                                                                                                  <  5:1716468/151‑1 (MQ=255)
    ggTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGTACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGaa                                                                                                                                                <  6:407929/151‑1 (MQ=255)
    ggTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGaa                                                                                                                                                <  2:319616/151‑1 (MQ=255)
    ggTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGa                                                                                                                                                 >  4:1291412/1‑150 (MQ=255)
       gtTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGc                                                                                                                                             >  3:1694792/1‑151 (MQ=255)
         tGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAAGGCt                                                                                                                                            >  6:67925/1‑150 (MQ=255)
          gCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTAc                                                                                                                                          >  5:934014/1‑151 (MQ=255)
           cGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTAcc                                                                                                                                         >  1:1602204/1‑150 (MQ=255)
           cGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACg                                                                                                                                         <  7:1021185/151‑1 (MQ=255)
               aCATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTCCCGTATAAAGAGATCGAACGCTACGGCa                                                                                                                                      >  4:1711050/1‑150 (MQ=255)
                cATGCTGCGCGTCAATAGCGTTTCTGACGGACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGGGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCAtt                                                                                                                                    <  8:705830/151‑1 (MQ=255)
                   gctgcGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGa                                                                                                                                  <  7:674137/150‑1 (MQ=255)
                   gctgcGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGAt                                                                                                                                 >  5:455315/1‑151 (MQ=255)
                    ctgcGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATc                                                                                                                                >  4:292708/1‑151 (MQ=255)
                       cgcgGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTa                                                                                                                             <  3:478333/151‑1 (MQ=255)
                       cgcgGCAATACCGTTTCAGACGCACCGCTGATTATCGGTAGCCGTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCgga                                                                                                                             <  6:1355506/151‑3 (MQ=255)
                          ggCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAAcc                                                                                                                          >  7:530668/1‑151 (MQ=255)
                           gCAATACCGTTTCTGACGTACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCg                                                                                                                         <  6:165580/151‑1 (MQ=255)
                            cAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGt                                                                                                                        <  7:1169862/151‑1 (MQ=255)
                             aaTACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGtt                                                                                                                       >  2:837799/1‑151 (MQ=255)
                             aaTACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGtt                                                                                                                       >  7:948015/1‑151 (MQ=255)
                              aTACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTc                                                                                                                      <  1:1507112/151‑1 (MQ=255)
                              aTACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTc                                                                                                                      <  4:924330/151‑1 (MQ=255)
                                    tttCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTcgccgc                                                                                                                 <  5:549926/150‑1 (MQ=255)
                                       cTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGCCCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGa                                                                                                              >  8:1409124/1‑150 (MQ=255)
                                        tGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGa                                                                                                              >  2:702065/1‑149 (MQ=255)
                                         gACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGaa                                                                                                             <  4:571427/149‑1 (MQ=255)
                                          aCGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTa                                                                                                          <  8:1333812/151‑1 (MQ=255)
                                           cGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTa                                                                                                          >  1:498158/1‑150 (MQ=255)
                                           cGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTaa                                                                                                         <  3:722985/151‑1 (MQ=255)
                                            gCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAg                                                                                                        <  1:1282213/151‑1 (MQ=255)
                                             cACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAgg                                                                                                       <  4:478006/151‑1 (MQ=255)
                                             cACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAgg                                                                                                       >  5:727517/1‑151 (MQ=255)
                                              aCCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAgg                                                                                                       >  4:14119/1‑150 (MQ=255)
                                              aCCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATAGATCGTAACCGTTCGCCGCTGAAGTAAGGa                                                                                                      <  2:634832/151‑1 (MQ=255)
                                               ccGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGAc                                                                                                     <  7:603050/151‑1 (MQ=255)
                                                cGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACa                                                                                                    <  1:896816/151‑1 (MQ=255)
                                                 gCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGAc                                                                                                     >  1:1027103/1‑149 (MQ=255)
                                                   tGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACaga                                                                                                  >  5:1393103/1‑150 (MQ=255)
                                                    gATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACaga                                                                                                  >  4:1550616/1‑149 (MQ=255)
                                                    gATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACagaag                                                                                                <  3:1107206/151‑1 (MQ=255)
                                                        aTCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATg                                                                                             <  7:1359430/150‑1 (MQ=255)
                                                          cGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTg                                                                                          <  6:36750/151‑1 (MQ=255)
                                                            gTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGaa                                                                                        <  8:623190/151‑1 (MQ=255)
                                                            gTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGa                                                                                         >  4:228280/1‑150 (MQ=255)
                                                                ccTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGtt                                                                                     >  7:275883/1‑150 (MQ=255)
                                                                      tCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGaa                                                                               >  7:818364/1‑150 (MQ=255)
                                                                       cccTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGaaa                                                                              >  6:638322/1‑150 (MQ=255)
                                                                        ccTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGAGCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGAGGCTGAAGTTACTGAAAAc                                                                            >  2:954211/1‑151 (MQ=255)
                                                                        ccTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAAc                                                                            <  5:1035553/151‑1 (MQ=255)
                                                                           gCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACTGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtat                                                                         <  5:1303995/151‑1 (MQ=255)
                                                                           gCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtat                                                                         <  5:866919/151‑1 (MQ=255)
                                                                           gCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACta                                                                          <  4:1621481/150‑1 (MQ=255)
                                                                           gCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtat                                                                         <  2:109079/151‑1 (MQ=255)
                                                                           gCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGGGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtat                                                                         <  1:36724/151‑1 (MQ=255)
                                                                              aCTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtatta                                                                       <  6:1456469/150‑1 (MQ=255)
                                                                              aCTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACtatt                                                                        <  4:74975/149‑1 (MQ=255)
                                                                                 ccTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcg                                                                   <  6:854427/151‑1 (MQ=255)
                                                                                    gTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcgc                                                                  <  2:1436693/149‑1 (MQ=255)
                                                                                         gaccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGGACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGa                                                            >  1:1341944/1‑150 (MQ=255)
                                                                                         gaccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGa                                                            >  1:790378/1‑150 (MQ=255)
                                                                                           ccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAAcc                                                         >  1:1583249/1‑151 (MQ=255)
                                                                                           ccgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAAcc                                                         <  3:849258/151‑1 (MQ=255)
                                                                                               gtgACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACcgcg                                                      >  8:173955/1‑150 (MQ=255)
                                                                                               gtgACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGa                                                     <  8:1028835/151‑1 (MQ=255)
                                                                                                 gACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACg                                                   >  6:502080/1‑151 (MQ=255)
                                                                                                 gACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACg                                                   >  5:334315/1‑151 (MQ=255)
                                                                                                          aGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAata                                           <  1:116883/150‑1 (MQ=255)
                                                                                                              gtgCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCt                                      >  5:784985/1‑151 (MQ=255)
                                                                                                               tgCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCtt                                     >  2:1359921/1‑151 (MQ=255)
                                                                                                                gcgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTc                                    <  3:232887/151‑1 (MQ=255)
                                                                                                                 cgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTcg                                   >  1:549327/1‑151 (MQ=255)
                                                                                                                   cAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTcgcg                                 >  4:1151277/1‑151 (MQ=255)
                                                                                                                    aaGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCgcgc                                >  8:483615/1‑151 (MQ=255)
                                                                                                                    aaGCGCCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGATACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCgcgc                                >  5:1148762/1‑151 (MQ=255)
                                                                                                                        gcCAGTCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTACGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACt                            <  3:1440718/151‑1 (MQ=255)
                                                                                                                          cattCAAAAACCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTgg                          <  3:609578/148‑1 (MQ=255)
                                                                                                                          cAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTgg                          <  1:968320/151‑1 (MQ=255)
                                                                                                                            gTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAg                        <  8:850542/151‑1 (MQ=255)
                                                                                                                             tCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAgg                       >  6:1111296/1‑151 (MQ=255)
                                                                                                                              cAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGt                      <  2:1711198/151‑1 (MQ=255)
                                                                                                                                aaaaCCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTc                     >  8:124231/1‑150 (MQ=255)
                                                                                                                                  aaCCGTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGc                  <  2:1654234/151‑1 (MQ=255)
                                                                                                                                    ccGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGccc                >  7:388229/1‑151 (MQ=255)
                                                                                                                                      gTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTg              <  7:148420/151‑1 (MQ=255)
                                                                                                                                      gTGCCGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTg              <  7:1645986/151‑1 (MQ=255)
                                                                                                                                       tGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTgg             >  7:740575/1‑151 (MQ=255)
                                                                                                                                        gCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGc            >  1:721266/1‑151 (MQ=255)
                                                                                                                                         ccGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTGCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCt           <  5:1629836/151‑1 (MQ=255)
                                                                                                                                         ccGTATAAAAAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCt           >  1:1706253/1‑151 (MQ=255)
                                                                                                                                           gTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCttt         >  5:1260008/1‑151 (MQ=255)
                                                                                                                                           gTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCttt         >  5:1558570/1‑151 (MQ=255)
                                                                                                                                              taAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCATGGCTTTcgc      >  8:24115/1‑151 (MQ=255)
                                                                                                                                                  gagaTCGAACTCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCATCCCTGGCTTTCGCGGaa  <  4:94953/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
GGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAA  >  NC_000913/2609961‑2610257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑