Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG26.9% 738.2 / 82.4 405intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (80/213);  new base G (104/4);  total (187/218)
Fisher's exact test for biased strand distribution p-value = 2.13e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 424 total aligned reads displayed.

GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422279‑422579
                                                                                                                                                      |                                                                                                                                                      
 gTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACCCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTCTAACCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGCAAGTTATCCGCCCCCGtc                                                                                                                                                       >  2:813017/1‑151 (MQ=255)
       cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGgcctccgc                                                                                                                                                 >  2:1498748/1‑149 (MQ=255)
        gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtccgcctcc                                                                                                                                                >  6:1052360/1‑151 (MQ=255)
         tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGccc                                                                                                                                               >  8:616192/1‑151 (MQ=255)
          ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCTCGATCGTCTGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTcccc                                                                                                                                              <  6:121279/151‑1 (MQ=255)
           gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                             <  6:54919/151‑1 (MQ=255)
              tgttgtGCTGTTGATTGTCTGGATGTATAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGAGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                           <  6:789443/150‑1 (MQ=255)
               gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGTAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                         <  2:377154/151‑1 (MQ=255)
               gttgtGCTGTTGATTGGCGCGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCTCCTCCCCCaaaa                                                                                                                                         <  7:750955/151‑2 (MQ=255)
                 tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                        <  7:1320938/150‑1 (MQ=255)
                   tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGGTCCTCCCCCAAATATcg                                                                                                                                     >  7:1183163/1‑150 (MQ=255)
                    gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCGCCCCCCAATATCCt                                                                                                                                    >  7:1488782/1‑151 (MQ=255)
                    gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                    <  4:64390/151‑1 (MQ=255)
                    gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                    <  8:840578/151‑1 (MQ=255)
                     cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAATATCCtt                                                                                                                                   >  6:1750455/1‑151 (MQ=255)
                     cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCtt                                                                                                                                   >  5:1424720/1‑151 (MQ=255)
                              ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                           <  3:396588/150‑1 (MQ=255)
                                cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCTGatgatgcg                                                                                                                        >  3:1311686/1‑149 (MQ=255)
                                 tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCGCCTCCCCCCAATATCCTTCAGATGATGAGt                                                                                                                       >  4:1399453/1‑151 (MQ=255)
                                  ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                      <  6:188916/151‑1 (MQ=255)
                                      gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGGCTGCTCCCCCAGATATCCTTGAGATGATGAGTgctgg                                                                                                                  >  3:1637658/1‑147 (MQ=255)
                                       tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCt                                                                                                                 >  1:1075338/1‑151 (MQ=255)
                                        ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                 <  8:268917/150‑1 (MQ=255)
                                               ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGATCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                         <  8:122540/151‑1 (MQ=255)
                                                  ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGGTCCGGCATTAgggt                                                                                                       >  4:191869/1‑148 (MQ=255)
                                                  ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATAGATCACATGATGAGAGATCGAGCAGTAggggt                                                                                                      >  6:166398/1‑148 (MQ=255)
                                                    aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                    <  5:1594234/151‑1 (MQ=255)
                                                      gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCGTCAGCTGATGAGTGCTGCTGCATTAGGcgttggg                                                                                                  >  1:933087/1‑145 (MQ=255)
                                                       aTCGTCCGCTGGCTGAAAACCAGTCATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                 <  4:1234118/151‑1 (MQ=255)
                                                       aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATGCTTCTGATGATGAGTGATCCTGCATTTGGCTATGGc                                                                                                  >  3:1146183/1‑150 (MQ=255)
                                                             aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTgg                                                                                           >  4:1259859/1‑150 (MQ=255)
                                                             aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCGCCCCAAAATCCTTCAGATGATGAGTGATCCTGCAATAGGCTATGGCAAGGTg                                                                                            >  7:518653/1‑150 (MQ=255)
                                                                 ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAGATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                        <  1:1477282/150‑1 (MQ=255)
                                                                 ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                        <  3:651407/150‑1 (MQ=255)
                                                                  gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                      <  5:803147/151‑1 (MQ=255)
                                                                      aaaaGCAGTACTCCTTGCGTTCTCTAAGCCGGCTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                  <  2:293798/151‑1 (MQ=255)
                                                                      aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGGTATGGCAAGGTGATCAGAtttt                                                                                  >  3:1404556/1‑151 (MQ=255)
                                                                       aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCCATCAGATGATGAGTGATACTGCATTAGGGGATGGCAAGGTGATAAGAtttt                                                                                  >  7:1644269/1‑150 (MQ=255)
                                                                           cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGATGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                             <  6:973340/151‑1 (MQ=255)
                                                                           cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGACGGCCCCAAAGATACTTCAGATGATGAGTGATCATGAAGTATGATAAGGCTAGTTGATCATATTTtaatg                                                                              >  6:300836/1‑149 (MQ=255)
                                                                              tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGGGTGCTCCCCCAAATATAATTAACATGAGGAGTGATGCTGCATTAGGGTGAGGCAAGGGGATCTGATTTgaatcaaa                                                                           >  4:968896/1‑148 (MQ=255)
                                                                               aaTCCTTGCGTTCTGTAATCCGGTGCAGGAGGAAAGACTGATCCGGCTATTCCGAAAGGTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                         <  4:813793/151‑1 (MQ=255)
                                                                                 tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCGCCGCCCCCCCATATCCTTCAGATGATGAGTGATCCTGCATTTGGGGATGGCGAGGTGATGAGGTTTTCATCTGAgggg                                                                       >  8:108864/1‑151 (MQ=255)
                                                                                  ccTTGCGTTCTGTAAGCCGGTTCAGGAGGATATCCTGATCCTGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATTAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                       <  8:79939/150‑1 (MQ=255)
                                                                                  ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAGATCCTTGAGATGATGAGTGCTCCTGCCTTAGGCTATGGCATGGTGCTCAGATTTTGATGACAggggc                                                                      >  1:630430/1‑150 (MQ=255)
                                                                                     tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCATTAAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTGATCACAGGGGAAtt                                                                   >  4:1444473/1‑151 (MQ=255)
                                                                                      gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                  <  2:130204/151‑1 (MQ=255)
                                                                                       cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                 <  8:1135157/151‑1 (MQ=255)
                                                                                        gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCGTCAGATGATGTGTGATGCTGCGTTAGGCTATGGGAAGGTGAGCTGAGTTTCTTGACAGGGGATTTatg                                                                >  4:528582/1‑151 (MQ=255)
                                                                                        gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGGCGCCCCCAAAAATCCTTCAGAGGGTGCGTTATGCGGCATTAGGGGTTGGCAAGGTGGTGAGTTTTTCATCACAGGGGTAttttt                                                                >  3:245511/1‑148 (MQ=255)
                                                                                            tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCCGGCCCCCAAGATCCTGGAGATGTTGAGTGGTCCGGCGTTAGGCGAGGGCCAGGGGCTCAGCTTTTGATCACTCGGGCATTATGATGt                                                            >  1:1619333/1‑151 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                             <  1:1301238/149‑1 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                           <  8:1362780/151‑1 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                            <  1:987746/150‑1 (MQ=255)
                                                                                             gTAAGCCGGCTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                              <  6:652182/148‑1 (MQ=255)
                                                                                               aaGCCGGTTCAGGAGGAAATCCGGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                         <  5:935500/151‑1 (MQ=255)
                                                                                                aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                        <  7:262587/151‑1 (MQ=255)
                                                                                                aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                        <  3:488512/151‑1 (MQ=255)
                                                                                                aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                        <  4:1045471/151‑1 (MQ=255)
                                                                                                  ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                      <  1:1559343/151‑1 (MQ=255)
                                                                                                    ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                    <  2:960892/151‑1 (MQ=255)
                                                                                                       tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                  <  1:309967/150‑1 (MQ=255)
                                                                                                       tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                  <  1:1228044/150‑1 (MQ=255)
                                                                                                        cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCATTCAGATGATGAGTGATCCTGCATTAGGCTTTGGGAAGGTGATCAGATTTTCATAACAGGGGAATTATTATGTTTAATTCATGGc                                                >  3:544471/1‑151 (MQ=255)
                                                                                                         aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGGCGCCCCCAAAAATCCGTCAGATGATGAGTGATCCGGCAGTAGGCTATGGGAAGGTGATAAGATTTTCATAACAGGGGAATTTTGATTTTAATTTAATGGc                                                >  8:455109/1‑150 (MQ=255)
                                                                                                         aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                               <  1:667706/151‑1 (MQ=255)
                                                                                                            cggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                            <  7:94682/150‑1 (MQ=255)
                                                                                                            aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCAGTAGGGTATGGCAAGGTGATCAGATTTTGATAACAGGGGAGTTATGATGTTAAATGGATGGccccg                                            >  4:1619313/1‑150 (MQ=255)
                                                                                                             ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                           <  4:316087/151‑1 (MQ=255)
                                                                                                               aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGATTGATCCTGGATTAGGGTATTGCAACGTGATCAGATTTTCATCACAGGGGAATTATGATTTTTAATTAATCGCACCTccc                                         >  5:1045036/1‑151 (MQ=255)
                                                                                                               aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                         <  2:1474007/151‑1 (MQ=255)
                                                                                                                  tCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGGCCGCCGCCCCCAAATAACCTTCAGATGATGAGTGATCCTGCATTAGGATATGGCAAGGTGATCAGAGTTTCATGACAGGGGAATTATGATGTTAAATGAATTGAAACTGCCgcg                                      >  1:567768/1‑149 (MQ=255)
                                                                                                                   ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTTTGATGTTAAATGCATGGCCCCTGCCGGTg                                     >  1:751643/1‑151 (MQ=255)
                                                                                                                      gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccc                                  <  2:1654490/151‑1 (MQ=255)
                                                                                                                      gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCGTCAGATGATGAGTGATGGTGCAGTAGGCTATGGGAAGGGGAGCAGATTTTGATGAACGGGGAATTATGATGTTAAATGCATGGAAACTCCCGGGGcc                                   >  7:893960/1‑150 (MQ=255)
                                                                                                                       aTCCGGCTATTCCGAAAGTTATCCGCGCCCCGCCGCCTCCCCCAAATATCATTCACAAGATGAGTGTTCCTGAATTAGGCTATTGAAACGTGATCAGATATTCATCAGACGGGATTTATTAATTTATATGAATGGAACCTCCCGGTGcccc                                 >  8:681485/1‑151 (MQ=255)
                                                                                                                         ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAAAACTTCAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATGACAGGGGAATTATGATTTTAAATGAATGGCACCTGCCGGTGcccccc                               >  5:3800/1‑150 (MQ=255)
                                                                                                                         ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCTCCCCCAAATATCCTTAAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTCATCACATGGGAATTATTATTTTAAATTCTTGGCACCTGCCGGTGccccc                                >  1:256058/1‑150 (MQ=255)
                                                                                                                          cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                              <  5:1126456/151‑1 (MQ=255)
                                                                                                                           ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                             <  8:1609588/151‑1 (MQ=255)
                                                                                                                           ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                             <  1:1005733/151‑1 (MQ=255)
                                                                                                                                ttCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATACAACAGATGATGAGTGAGCCTGGAATAGGCTAAGGCAAGGTGATCAGATTTTCATAAAAAGGGAATTATGATGTTAAATGACTTGCCACAGGCGGTGCCCCCATTAGtgt                        >  2:596425/1‑149 (MQ=255)
                                                                                                                                  ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaaa                      <  5:1249613/151‑1 (MQ=255)
                                                                                                                                  ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                       <  5:1516334/150‑1 (MQ=255)
                                                                                                                                    cacacatATCCGCCCCCCGCCTCCCCCCCCAAAAATCCTCCAGATGATGTGAGATCCTGCATTTGCCTATCGCAAGCTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                    <  5:535905/145‑1 (MQ=255)
                                                                                                                                     aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                   <  2:451052/151‑1 (MQ=255)
                                                                                                                                       aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATAAAAGGGGAATTATTATTTTAAATTAATGGCACCTGCCGGTGCCCCCAATTTTTAAAcaaa                  >  5:290154/1‑150 (MQ=255)
                                                                                                                                           aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATCCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa             <  3:884303/151‑1 (MQ=255)
                                                                                                                                             ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag            <  3:1028642/150‑1 (MQ=255)
                                                                                                                                             ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag            <  5:1729658/150‑1 (MQ=255)
                                                                                                                                              cGCCCCCGTCCTCCTCCCGCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATTGCAAGGTGATCAGATTTTCATGACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt          <  2:722562/151‑1 (MQ=255)
                                                                                                                                               gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGGCCCCATTTGTTAAACAAAGCAAAGAt          >  7:616969/1‑150 (MQ=255)
                                                                                                                                                cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGCTCCTGCAGTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATcc        >  7:1368865/1‑150 (MQ=255)
                                                                                                                                                  cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      >  1:860161/1‑151 (MQ=255)
                                                                                                                                                  cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      >  3:1533245/1‑151 (MQ=255)
                                                                                                                                                   ccGTCCTCCTCCCGCAAATATCCTTGAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  5:73658/151‑1 (MQ=255)
                                                                                                                                                   ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     >  8:354649/1‑151 (MQ=255)
                                                                                                                                                    cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    >  7:309855/1‑151 (MQ=255)
                                                                                                                                                    cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    >  6:1462941/1‑151 (MQ=255)
                                                                                                                                                     gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   <  7:1083469/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422279‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑