Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4320TG22.3% 907.9 / 61.9 405intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (96/218);  new base G (90/0);  total (187/218)
Fisher's exact test for biased strand distribution p-value = 9.50e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAT  >  NC_000913/422284‑422582
                                                                                                                                                    |                                                                                                                                                      
  ggTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcctc                                                                                                                                                    <  2:566061/150‑1 (MQ=255)
    tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTGCTccc                                                                                                                                                  >  2:369315/1‑151 (MQ=255)
    tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccc                                                                                                                                                  >  5:1488062/1‑151 (MQ=255)
     ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccc                                                                                                                                                  <  1:314081/150‑1 (MQ=255)
      gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                                <  6:54919/151‑1 (MQ=255)
        ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCa                                                                                                                                               >  7:1642000/1‑150 (MQ=255)
         tgttgtGCTGTTGATTGTCTGGATGTATAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGAGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                              <  6:789443/150‑1 (MQ=255)
         tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCaa                                                                                                                                              >  8:1542909/1‑150 (MQ=255)
          gttgtGCTGTTGATTGGCGCGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCTCCTCCCCCaaaa                                                                                                                                            <  7:750955/151‑2 (MQ=255)
           ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAat                                                                                                                                            >  3:1079001/1‑150 (MQ=255)
             gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                         <  1:1483759/151‑1 (MQ=255)
              tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGGTCCTCCCCCAAATATcg                                                                                                                                        >  7:1183163/1‑150 (MQ=255)
              tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCACCCGGCCTCCTCCCCCAAATATc                                                                                                                                         >  6:987054/1‑150 (MQ=255)
               gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                       <  8:840578/151‑1 (MQ=255)
                      aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATCTCCTTCAga                                                                                                                                  >  4:263984/1‑149 (MQ=255)
                          gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCGCCGCCCCCCAAGATCCTTCAGcggatg                                                                                                                             >  5:1270579/1‑150 (MQ=255)
                          gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                            <  2:1460051/151‑1 (MQ=255)
                          gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                            <  3:1223528/151‑1 (MQ=255)
                               aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGGGGGCCCCACATATCCGTCAGCTGATGAggcat                                                                                                                       >  6:394205/1‑147 (MQ=255)
                                tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGGAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTGCTCCCCGCACTATCCTTCAGATGATGGGTGCTc                                                                                                                      >  1:781640/1‑151 (MQ=255)
                                 gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGGCTGCTCCCCCAGATATCCTTGAGATGATGAGTgctgg                                                                                                                     >  3:1637658/1‑147 (MQ=255)
                                  tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCt                                                                                                                    >  1:1075338/1‑151 (MQ=255)
                                   ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                    <  8:268917/150‑1 (MQ=255)
                                     aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCCGTCAGAGGCTGAGtgctgctgcg                                                                                                                 >  8:1085370/1‑150 (MQ=255)
                                       aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCGCCGCCCCCAAATATACGTGAGATGATGGGTGATcgggcagt                                                                                                               >  4:829440/1‑144 (MQ=255)
                                         gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCGTCCTCCCCCACATATCGTTCCGATGATGAGTGCTCCTGGAtt                                                                                                               >  5:1734173/1‑149 (MQ=255)
                                          ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGATCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                            <  8:122540/151‑1 (MQ=255)
                                                 gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCGCGGCCCCCCAATATCCTTCAGGTGATGAGTGATCCTGGATTTGGGTATggg                                                                                                     >  5:786368/1‑150 (MQ=255)
                                                 gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCGTCAGCTGATGAGTGCTGCTGCATTAGGcgttggg                                                                                                     >  1:933087/1‑145 (MQ=255)
                                                  aTCGTCCGCTGGCTGAAAACCAGTCATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                    <  4:1234118/151‑1 (MQ=255)
                                                    cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCATGCTGATGATGACTGATCCTGCATTATGCTATGGCa                                                                                                    >  4:533131/1‑149 (MQ=255)
                                                     gTCAGCTGGCTGAAAACCATTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                                 <  6:150820/151‑1 (MQ=255)
                                                        aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTgg                                                                                              >  4:1259859/1‑150 (MQ=255)
                                                        aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCGCCCCAAAATCCTTCAGATGATGAGTGATCCTGCAATAGGCTATGGCAAGGTg                                                                                               >  7:518653/1‑150 (MQ=255)
                                                            ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                           <  3:651407/150‑1 (MQ=255)
                                                            ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAg                                                                                          <  7:1388348/151‑1 (MQ=255)
                                                                gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAttt                                                                                      <  4:1327274/151‑1 (MQ=255)
                                                                gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCACCCCCAAATATCCTACAGATGATGAGTGATCGTGCAGTAGGCTTTGGGAAGGTGATCAGAttt                                                                                      >  2:1495591/1‑151 (MQ=255)
                                                                 aaaaGCAGTACTCCTTGCGTTCTCTAAGCCGGCTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                     <  2:293798/151‑1 (MQ=255)
                                                                          aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                            <  6:1070306/151‑1 (MQ=255)
                                                                          aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                            <  5:884743/151‑1 (MQ=255)
                                                                          aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGCGATGGGAAGGTGCTCGGATTTTGATCACAgg                                                                            >  3:822514/1‑151 (MQ=255)
                                                                          aaTCCTTGCGTTCTCTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATACATAACATAATTAGTGATCATGCATTAGCATATGTCAAAGTGATCAGAATTtaataaaagt                                                                            >  5:1045736/1‑145 (MQ=255)
                                                                           aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGAGGAGGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGCTTTTCATGACAggg                                                                           >  5:1547544/1‑151 (MQ=255)
                                                                            tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGTCCTCCGGCCCCAAAGATAGTAAAGATGATGAGGGATCGTGCAGTAGGCTATGGGAAGGGGAGGAGATTTTAATGACAgggg                                                                          >  4:974146/1‑151 (MQ=255)
                                                                             ccTTGCGTTCTGTAAGCCGGTTCAGGAGGATATCCTGATCCTGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATTAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                          <  8:79939/150‑1 (MQ=255)
                                                                             ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAGATCCTTGAGATGATGAGTGCTCCTGCCTTAGGCTATGGCATGGTGCTCAGATTTTGATGACAggggc                                                                         >  1:630430/1‑150 (MQ=255)
                                                                                tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                       <  5:987217/150‑1 (MQ=255)
                                                                                 gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                                      <  7:1330276/150‑1 (MQ=255)
                                                                                 gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                     <  2:130204/151‑1 (MQ=255)
                                                                                  cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                    <  8:1135157/151‑1 (MQ=255)
                                                                                   gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGGCGCCCCCAAAAATCCTTCAGAGGGTGCGTTATGCGGCATTAGGGGTTGGCAAGGTGGTGAGTTTTTCATCACAGGGGTAttttt                                                                   >  3:245511/1‑148 (MQ=255)
                                                                                     tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                                  <  6:1229845/150‑1 (MQ=255)
                                                                                       tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCCGGCCCCCAAGATCCTGGAGATGTTGAGTGGTCCGGCGTTAGGCGAGGGCCAGGGGCTCAGCTTTTGATCACTCGGGCATTATGATGt                                                               >  1:1619333/1‑151 (MQ=255)
                                                                                        gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                                <  1:1301238/149‑1 (MQ=255)
                                                                                        gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                                 <  2:420101/148‑1 (MQ=255)
                                                                                        gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                              <  5:564391/151‑1 (MQ=255)
                                                                                        gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                              <  8:1362780/151‑1 (MQ=255)
                                                                                        gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                               <  1:987746/150‑1 (MQ=255)
                                                                                        gTAAGCCGGCTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                                 <  6:652182/148‑1 (MQ=255)
                                                                                           aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                           <  7:262587/151‑1 (MQ=255)
                                                                                           aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                           <  3:488512/151‑1 (MQ=255)
                                                                                           aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                           <  4:1045471/151‑1 (MQ=255)
                                                                                             ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                         <  1:1559343/151‑1 (MQ=255)
                                                                                               ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                       <  2:960892/151‑1 (MQ=255)
                                                                                                 ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGGCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATGAGAGTTTGATGACAGGGGGATTATGATTTTAAAttgatg                                                     >  4:662399/1‑146 (MQ=255)
                                                                                                  tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                     <  1:1228044/150‑1 (MQ=255)
                                                                                                  tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCGCCGCCCCCCAATATCCTTCAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTTAatggatgg                                                    >  4:1645457/1‑151 (MQ=255)
                                                                                                   cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCATTCAGATGATGAGTGATCCTGCATTAGGCTTTGGGAAGGTGATCAGATTTTCATAACAGGGGAATTATTATGTTTAATTCATGGc                                                   >  3:544471/1‑151 (MQ=255)
                                                                                                    aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGGCGCCCCCAAAAATCCGTCAGATGATGAGTGATCCGGCAGTAGGCTATGGGAAGGTGATAAGATTTTCATAACAGGGGAATTTTGATTTTAATTTAATGGc                                                   >  8:455109/1‑150 (MQ=255)
                                                                                                    aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGGGGTGAGATTTTGTTCACAGGGGAATTATGATGTTTTATGAATgtcg                                                  >  6:1755376/1‑148 (MQ=255)
                                                                                                    aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                                  <  1:667706/151‑1 (MQ=255)
                                                                                                       cggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                               <  7:94682/150‑1 (MQ=255)
                                                                                                         gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAGATACTTCAGNTGATGATNGATCGTGGATTAGGGGATGGNAAGGGGATGAGATTTTATTAACAGGGGAATTNTGATNTTTAATGCATTGCTCCTGc                                             >  5:1625924/1‑151 (MQ=255)
                                                                                                          aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCCAAAATCCTTCAGATGATGACTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATCCTTGGCACCTGcc                                            >  3:373303/1‑151 (MQ=255)
                                                                                                           aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCg                                           <  5:1193677/151‑1 (MQ=255)
                                                                                                             tCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGGCCGCCGCCCCCAAATAACCTTCAGATGATGAGTGATCCTGCATTAGGATATGGCAAGGTGATCAGAGTTTCATGACAGGGGAATTATGATGTTAAATGAATTGAAACTGCCgcg                                         >  1:567768/1‑149 (MQ=255)
                                                                                                             tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATCATTCAGATGATGAGTGGTGCTGCATTAGGGTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATTCATGGCACCTGCCggg                                         >  3:1335891/1‑150 (MQ=255)
                                                                                                              ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                         <  5:328912/150‑1 (MQ=255)
                                                                                                                 gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCGTCAGATGATGAGTGATGGTGCAGTAGGCTATGGGAAGGGGAGCAGATTTTGATGAACGGGGAATTATGATGTTAAATGCATGGAAACTCCCGGGGcc                                      >  7:893960/1‑150 (MQ=255)
                                                                                                                 gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAAACAATCAGATGATGAGTGATCCTGCAGTAGGCTATGGGAAGGTGATCAGAATTTCATAAAAGGGGAATTATGATTTTAAATGAATGTCAACTGCCGGTGccc                                     >  2:789342/1‑151 (MQ=255)
                                                                                                                  aTCCGGCTATTCCGAAAGTTATCCGCGCCCCGCCGCCTCCCCCAAATATCATTCACAAGATGAGTGTTCCTGAATTAGGCTATTGAAACGTGATCAGATATTCATCAGACGGGATTTATTAATTTATATGAATGGAACCTCCCGGTGcccc                                    >  8:681485/1‑151 (MQ=255)
                                                                                                                    ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCTCCCCCAAATATCCTTAAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTCATCACATGGGAATTATTATTTTAAATTCTTGGCACCTGCCGGTGccccc                                   >  1:256058/1‑150 (MQ=255)
                                                                                                                      ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                                <  1:1005733/151‑1 (MQ=255)
                                                                                                                                 aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGCGTGATCCTGCATTAGGCTATGGCAAGCTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                     <  8:608403/151‑1 (MQ=255)
                                                                                                                                  aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                    <  7:605743/151‑1 (MQ=255)
                                                                                                                                    ttATCCGCCCCCGGCCTCCTCCCCCAAATATCCGTGAGATGATGAGTGATGCTGCATTAGGGTATGGCAAGGTGATCAGATTTTAATCACAGGGGGATTATGATGTTAAATGAATGGCACCTGCCGGGGGCCCCCGTTGTTTAAcaaacc                   >  2:1601745/1‑148 (MQ=255)
                                                                                                                                    ttATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATGATGCATTAGGATATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATTATGTTAAATGAAAGGCACCTGCCGGGGCCCCCATTTGTTTAAcaaaga                   >  6:1675482/1‑149 (MQ=255)
                                                                                                                                     tATCCGCCCCCGTCCTCCTACCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATCGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca                  <  4:1523333/150‑1 (MQ=255)
                                                                                                                                      aTCCGGCCCCGTCCTCCTGCCCCAAATATACTTGAGATGATGAGGGATGCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTATATGAATGGCCCCTGCCGGGGCGCCCCGTTGTTAAAcaaagcaa                 >  7:1514047/1‑150 (MQ=255)
                                                                                                                                         cGCCCCCGTCCTCCTCCCGCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATTGCAAGGTGATCAGATTTTCATGACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt             <  2:722562/151‑1 (MQ=255)
                                                                                                                                           cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa           <  5:394944/151‑1 (MQ=255)
                                                                                                                                            ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa          <  4:342086/151‑1 (MQ=255)
                                                                                                                                             cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc         >  3:1533245/1‑151 (MQ=255)
                                                                                                                                             cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc         >  1:860161/1‑151 (MQ=255)
                                                                                                                                              ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt        >  8:354649/1‑151 (MQ=255)
                                                                                                                                               cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg       >  7:309855/1‑151 (MQ=255)
                                                                                                                                                gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc      <  7:1083469/151‑1 (MQ=255)
                                                                                                                                                    tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAt  >  3:1393330/1‑151 (MQ=255)
                                                                                                                                                    tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAGCACCTGCTCAt  >  6:211600/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAT  >  NC_000913/422284‑422582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑