Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913696,5340GT25.9% 311.5 / 39.2 144intergenic (‑30/+8)glnX/glnVtRNA‑Gln/tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base G (44/62);  new base T (10/27);  total (55/89)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCG  >  NC_000913/696385‑696684
                                                                                                                                                       |                                                                                                                                                      
gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAGAGCGTGCACTTCCAAGGTAA‑GCG‑ACTTg                                                                                                                                                        >  5:1021806/1‑150 (MQ=255)
gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGa                                                                                                                                                       <  4:1234161/151‑1 (MQ=255)
     cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATaaa                                                                                                                                                  <  6:178944/151‑1 (MQ=255)
      aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAAt                                                                                                                                                 <  5:564932/151‑1 (MQ=255)
       caaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAAtt                                                                                                                                                <  1:356576/150‑1 (MQ=255)
        aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTg                                                                                                                                               <  5:498714/151‑1 (MQ=255)
        aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTg                                                                                                                                               <  5:361298/151‑1 (MQ=255)
        aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTg                                                                                                                                               <  7:1745378/151‑1 (MQ=255)
        aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTg                                                                                                                                               <  8:402400/151‑1 (MQ=255)
        aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTg                                                                                                                                               <  3:1199075/151‑1 (MQ=255)
        aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGCTAA‑GCG‑TCTTGAATAAATTg                                                                                                                                               <  5:584162/151‑1 (MQ=255)
         aaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTgg                                                                                                                                              <  2:1630778/151‑1 (MQ=255)
           aGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCt                                                                                                                                            <  3:791866/151‑1 (MQ=255)
           aGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACACCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCt                                                                                                                                            <  2:699985/151‑1 (MQ=255)
            gCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTg                                                                                                                                           <  7:1685903/151‑1 (MQ=255)
            gCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTg                                                                                                                                           <  5:1409736/151‑1 (MQ=255)
             cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTgg                                                                                                                                          <  8:938099/151‑1 (MQ=255)
              aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTggg                                                                                                                                         <  1:396050/151‑1 (MQ=255)
              aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTggg                                                                                                                                         <  7:276220/151‑1 (MQ=255)
               aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGTCTGGGGTACGATGATTCGAACCTCGGAATGCCGTAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTtggg                                                                                                                                        <  4:1461394/151‑1 (MQ=255)
                aaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGt                                                                                                                                       <  3:511010/151‑1 (MQ=255)
                aaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATACCGGAATCAAAATCAAGTGACTTAACGCATGGCGATAACACAACTGTGTGCATTTAAATTATAA‑TAA‑TCTTGAATATAGTGGCTGGGGt                                                                                                                                       >  7:1577415/1‑151 (MQ=16)
                 aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCATGAAAAAGACTCCGGTGCCTTACAGATTGGCGATACCCCAACAGATTGCAGTTACAAGGTAA‑TCA‑TATATAATAAATAGGCTGGGGtg                                                                                                                                      >  6:1361120/1‑150 (MQ=18)
                 aaGCTCGCCGAAGCGAGCTTTTTTAATGGGGCGGGGGTACGAGGAATCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTa                                                                                                                                      <  1:1477337/151‑1 (MQ=255)
                  aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTAc                                                                                                                                     <  2:1379642/151‑1 (MQ=255)
                  aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTAc                                                                                                                                     <  2:1063142/151‑1 (MQ=255)
                     tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGCATCCGGTGCCTAACCGCTTGGCGATACCGCAGCTGGGTGCTGGTACAAGTTAA‑GCG‑GCTTGGAGTAAGTGGCTGGGGtggggg                                                                                                                                  >  5:546580/1‑146 (MQ=18)
                      cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAgg                                                                                                                                 <  3:1722341/151‑1 (MQ=255)
                       gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACATCTTGCAATTACAAGGTAA‑GAC‑TCTATAATAAATAGTCAGAAGTACGAgg                                                                                                                                 >  6:1012026/1‑150 (MQ=17)
                        ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTTAATAAATTGGCTGGGGTACGAGGAt                                                                                                                               <  4:237647/151‑1 (MQ=255)
                        ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGAt                                                                                                                               <  8:302126/151‑1 (MQ=255)
                         cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGTTTCGAAACTGGGAATGCCGGAATCAGAATCCGGTGCCTTACAGCTTGGCGATACCCCAACTTGGTGCACTTACAAGGTAA‑GCG‑TCTTTAATAAATTGGCTGGGGTACGAGGAtt                                                                                                                              <  6:199092/151‑1 (MQ=25)
                         cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                                                                                              <  4:1125778/151‑1 (MQ=255)
                           acgCGAGCTTTTTTAATGTGGATGGGGTACGAGGTTTCGATACTCGGAATGCCGGAATGAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACCAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                                                                                            <  4:1691964/149‑1 (MQ=25)
                            accGAGCTTTTTTAATGTGGCTGGGGTAGGTGGTTTCGATACTCGGAATGACTGAATCAGTATCCTTGGCCTTACCGCTTGTCGATACGCAAACTGGGTTCACTTACAAGGTAC‑GCT‑TCTTTAATAAATTGGCTGGGGTACGAGGATTCGa                                                                                                                           <  6:438307/149‑1 (MQ=255)
                            aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGATTGCAATTACAATTTAA‑GAG‑AATTTAATAAAAATGCTGAAGTACGAGGATACg                                                                                                                            >  6:1589296/1‑150 (MQ=14)
                             gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGATTCAGAGGACGGTGCGTAACAGCTTTGCCATTCCCCAACTGCGTCGACTTTCATGTGAA‑GCA‑AATATAATAAAGTGGCTGGGGTaggtggctgcg                                                                                                                            >  8:153875/1‑139 (MQ=12)
                                aggttttttTATGTTGATGGGGTAGGTTGTTTCGATACTTGGAATGCTTGTATCAGAATACTGTGCATTAGAGATTGTCTTTACGCCTACTTGGTTCAATTTCTAGGTAA‑GCT‑TCTTTATTAATTTGGCTGGGGTACGAGGATTCGAACCt                                                                                                                       <  7:1226891/148‑1 (MQ=255)
                                 ggttttttAATGTGGCTGGGGTACGATGAATCGAACCTCGGAATGCCTGAATCAGAATCCGGTGCCTTACCGCTTGGCTATACCCCAACTGGGTTCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                                                                                                      <  2:1484783/149‑1 (MQ=25)
                                 gCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCAAACTGCGTCCACTTACAAGGTAA‑GCC‑TCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTc                                                                                                                      >  5:414378/1‑151 (MQ=255)
                                  gttgttttATGTGGCTGGGGTACGCGGCGTCGATACTCTGAATGCCCTCATCAGTCTCCTGTGCATTAGCGCTTGTCGATACCCCAACTCGGTGCACTTTCTACGTAA‑GCC‑TCTTTTATAAATTGGCTGGGGTACGAGGATTCGAACCTCg                                                                                                                     <  2:1021445/147‑1 (MQ=255)
                                    tttttAATGTGGCGGGGGGACGAGGATTCGACACTCTGAATGCCGCAATCAGAATCCTGTGCCTTACCGCTTGGCTATACCCCAACTCGGTGCACTTACAAGGTAA‑GCT‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCg                                                                                                                     <  8:610499/149‑1 (MQ=255)
                                    tttttAATGTCGCTGGGGGACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAGCGCTTGGCGATACCCCAACTCGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCgg                                                                                                                    <  1:134212/150‑1 (MQ=255)
                                       ttAATGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGAAGCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAAt                                                                                                                 <  3:707389/150‑1 (MQ=255)
                                       ttAATGTGGCGGGGGTACGAGGACTCGCCCCTCGGAATGCCGGAAGCAGAATCCCGCGCCTTACCGCTTGCCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGTATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAAt                                                                                                                 <  1:1101469/150‑1 (MQ=255)
                                        tAATTTGGCTGGGGTACGAGGAGTCGAACCTCGGAATGCCGGAATCAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCT‑GCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATg                                                                                                                <  5:331118/150‑1 (MQ=255)
                                         aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGCGTGGTGTTGAATTGTCC‑GCC‑GCTATTAGATAGTGGCTGGGGTACGAGGATTCGacgggcggcgtgcc                                                                                                              >  8:870782/1‑138 (MQ=0)
                                           tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGAAATCAGAATCCCGTCCATTACCGAAAGACTATAAACCAACTTGTTGATCTAACAAGTAAA‑GCT‑AATATAAAAAAATTACTGGGGTACGAGGATTCGAACGGAGGAATGCCgg                                                                                                            >  2:1446753/1‑151 (MQ=9)
                                           tgtcgagggggTACGAGGATTCGACCCTCTGAATGACGCAATCAGAATCCCGTGCCTTACCGCTTGGCGCTACGCCCACTGGGTGCACTTACCAGGTAA‑GCT‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                                                                                             <  4:327779/143‑1 (MQ=37)
                                             tgGCGGGGGGACGCTGACTCGATCCTGGGAATGCCGCAATCAGAATCCGTCGCCTTACCGCTTGGCTATACCCAAAATCGCAGAACTTACAAGGTAACGCG‑‑CTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                                                                                                           <  1:1149641/150‑1 (MQ=255)
                                               gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAGTTACAAGTTAA‑GCG‑GATTGAATAAATTGGCTGGTGTACGAGGCTTCGAACCTCGGCAAGCCGGAGTc                                                                                                        >  8:1416020/1‑151 (MQ=21)
                                                  ggggTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                                                                                                       <  5:714878/149‑1 (MQ=255)
                                                    gggaggAGGATTCGCTACTCTGAAGGCCGCAAGGAGTCTCCGTGGCCTTCCCGCTTGTCGATACCCCCCCAGGGTTCACGTACAAGGT‑A‑GCGCGCTTTAATAAAGTGGCTGGGGTAGGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                                                   <  1:631232/146‑1 (MQ=9)
                                                      tACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGTAATGCCTGTATCAGAATcc                                                                                                 >  2:277594/1‑151 (MQ=255)
                                                      tACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAAGTGCACTTGCAAGGGAA‑GCG‑GATATAAGATAGTTGCTGGGGTACGTGGATACGAAGCTATTAGTGCCGGAGTCACAATcc                                                                                                 >  8:1281722/1‑151 (MQ=9)
                                                       cccccgATCCGCACCTCCGAATGCCGGAATCAGTTTTTTTTTATTTTCCGCCTCGCTATAACCCAAATTGTTGCTATTACAATGTAT‑CCG‑TATTGTATTATTTTCCTTTCTTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                                                                                                <  7:85361/146‑1 (MQ=2)
                                                         gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                                              <  5:1311302/151‑1 (MQ=255)
                                                         gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGAGCCTTACCGCTTGGCGATACCCCAACAGGGTGCAATTAAATTTTAA‑GCG‑GCTAGAAGAAAGAGTCTGCGGTTCGAGGCTACGAACCTCGGCCTGCCTGAGACAGCATccttt                                                                                              >  5:737330/1‑148 (MQ=9)
                                                           ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCTCTTGGCGATACACCACGTCGCTGCAGTTACATTGGAC‑GCG‑GGGTTAATAATTATTCTGCTTTAAGAGTATTCGACCCTCGGCGTGCCGGGGTCCGCATCCGGTgg                                                                                            >  3:497679/1‑150 (MQ=2)
                                                               tCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTTGGTGGACTTAAAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACGGCGGATTGCCGGTCTCAGAATCCGGTGCCttg                                                                                        >  5:199478/1‑150 (MQ=25)
                                                                           aTGCCTGCATCAGAATCCCTTGCCTTAGAGATTGGAGATACCCCAACTCGGATCACTTACTAGGTAA‑GCT‑GCTTTTTTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                            <  1:1157998/151‑1 (MQ=17)
                                                                            ggaccacagcAGACTCCGGAGCCTTCCCGCTTGCCTATACCCCAACTCGGACCACTTACAAGGTAC‑GCC‑GCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                           <  5:1365702/142‑1 (MQ=18)
                                                                               cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTCCGTGCAGTTGCAAGGGAA‑GCG‑GCTTGAAGAAATAGGCTGTGGTAGGAGGCTTCGAACCTCTGGGTGCCGGACACAGCATCAGGTCCCTTACCGcattcggacaccc                                                                        >  5:784738/1‑139 (MQ=16)
                                                                                 gAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAGTTACAATGTAA‑GAG‑GCTATAATAAATAGTCTGCGGTAGGAGGATTCGACCCTCGGAGAGCCTGAGACAGAATCAAGAGCCTTACCGCTGGCCGCTGccccc                                                                      >  5:1115002/1‑150 (MQ=16)
                                                                                              gTGCCTTACCGCTTGGAGCAACCCCCACATGGAGAACGTACCAGGTAC‑CCT‑TCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGtttt                                                         <  5:1675290/151‑1 (MQ=17)
                                                                                                  cTTACCGCTTGGCGATACCCCAACTGGGTGCTCTTACATTGTAA‑GCG‑TCTTGAATAAATTTGCTGGGGTACGAGGATTCGAAGGTCTGCATGCCTGATTCAGAATCCTGTGGCTTACAGCTTGGGGATACCCCAAGAAATTGCTTTTGatt                                                     >  8:1460039/1‑149 (MQ=255)
                                                                                                    tACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCaaaa                                                                                                    >  4:849542/1‑102 (MQ=255)
                                                                                                      ccGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTCCCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                                                 >  1:911358/1‑151 (MQ=255)
                                                                                                      ccGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                                                 >  8:193934/1‑151 (MQ=255)
                                                                                                         cTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAATCCGGTGCCTTACCGCTTGGCGAt                                                                            >  4:1360810/1‑121 (MQ=255)
                                                                                                           tGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCAGAATCAGAATACGGTGCCTTACCGCTTGGAGAGACACCACCAAATTGGTTTTGAATTTGCCGaa                                             >  5:1750770/1‑150 (MQ=37)
                                                                                                            ggCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAGTCAGAATCCGGTGCCTTACCGCGGGGCGATACCCCAATAACCTGGTGGTGaaaatctcgctcg                                           >  1:1369839/1‑140 (MQ=21)
                                                                                                            ggCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc                                            >  6:83145/1‑150 (MQ=255)
                                                                                                              cgttACCCCTACTTGGTTCACTTACAAGGTAT‑GCT‑TCTTTAATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata                                         <  2:287233/148‑1 (MQ=21)
                                                                                                               gATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACAAATTGGTTTTGAATTTGCCGAACatat                                        >  2:1515650/1‑151 (MQ=255)
                                                                                                                   ccccAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCt                                                                                          >  2:476656/1‑97 (MQ=255)
                                                                                                                       aactcgcttcACTTACTAGGAAT‑GCT‑TCTTTTATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa                                <  6:675651/142‑1 (MQ=18)
                                                                                                                       aaCTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAAAAACCGGTg                                                                                             >  4:1212312/1‑90 (MQ=255)
                                                                                                                          tGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATATATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCTGTGCCTTACAGCTTGGGGATACCCCAACAAATTGGTTTTGAATTTGCAGAACATATTCGATCCATTc                             >  8:1678842/1‑151 (MQ=25)
                                                                                                                           gggTTCACTTACTACGTAA‑GGC‑TCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTc                             <  5:1591832/150‑1 (MQ=21)
                                                                                                                           gggTGCACTTACCAGGTAA‑GCG‑TCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTc                             <  1:577706/150‑1 (MQ=255)
                                                                                                                                cACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAAtt                       >  6:415754/1‑151 (MQ=255)
                                                                                                                                 aCTTACAATGTAA‑TGC‑TCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCGATa                                                                           <  3:1110370/98‑1 (MQ=11)
                                                                                                                                  aatgtcaccgaacgCG‑TCTTTTATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAAttt                      <  8:442502/138‑1 (MQ=21)
                                                                                                                                       aaGGTAA‑GGG‑TCTTGAATAAATTGGCTGGGGTACGTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                                                                 <  3:899444/86‑1 (MQ=11)
                                                                                                                                       aaGGTAA‑GCG‑TCTTGAATAACTTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCcaataaccggg                                                             <  1:1499282/106‑6 (MQ=14)
                                                                                                                                       aaGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaataaccgg                                                              >  7:1114650/1‑101 (MQ=255)
                                                                                                                                       aaGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATaaacggg                                                             >  2:1496847/1‑102 (MQ=255)
                                                                                                                                       aaGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTGGCGATACCCcaataaccgg                                                              <  8:1116614/105‑5 (MQ=17)
                                                                                                                                       aaGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAATCCGGTGCCTTACCGCTTg                                                                                 >  4:897870/1‑86 (MQ=255)
                                                                                                                                         ggTAA‑GCT‑GCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTa              <  8:1394938/151‑1 (MQ=255)
                                                                                                                                            aa‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGATTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTacg            >  2:97321/1‑150 (MQ=255)
                                                                                                                                               gCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTacgacg         >  3:1147299/1‑151 (MQ=255)
                                                                                                                                                cG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                                                                 <  4:172587/78‑1 (MQ=14)
                                                                                                                                                cG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                                                 >  3:172784/1‑78 (MQ=255)
                                                                                                                                                cG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaata                                                                   >  3:1045099/1‑90 (MQ=255)
                                                                                                                                                cG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGACATAAGAAGAGACAGGTGGATTAAAACATGGAGATACCCAATCAAATTGGTTTTGAATTTGCGTAACATATTAGATAAATTCAGAATTTGGTGGGTAGGACgg        >  6:659676/1‑151 (MQ=255)
                                                                                                                                                 cttCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCGATACCCcaata                                                                   <  4:1043415/90‑3 (MQ=14)
                                                                                                                                                   tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACggg       >  7:932778/1‑150 (MQ=255)
                                                                                                                                                      tGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACACcatacg                                                                  >  8:1618801/1‑84 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAA‑GCG‑TCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCG  >  NC_000913/696385‑696684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑