Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,518,1420CG21.7% 675.2 / 52.4 332intergenic (‑26/+14)alaX/alaWtRNA‑Ala/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (142/118);  new base G (27/45);  total (169/163)
Fisher's exact test for biased strand distribution p-value = 1.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 335 total aligned reads displayed.

CAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAATGTGTCA  >  NC_000913/2517992‑2518291
                                                                                                                                                      |                                                                                                                                                       
     aGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCACCACGATGCGTTTACGTACCAAGTTTGCTGGGt                                                                                                                                                    <  4:475464/151‑1 (MQ=255)
           gaCAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCTGGATCGAACCGCTGACCTATTGCATGCCATGCAAGCTCTCTCCCATCAGAGCTATAGCGCCTCGATTCGTTTACGTACCAAGTTTGCTGGGTTCa‑aaa                                                                                                                                             <  2:41889/151‑1 (MQ=255)
              aaaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAGGAAGAGATTAATTTCCAGTTGAGCTGCGTGCT‑AAAt‑tt                                                                                                                                         >  4:869627/1‑151 (MQ=17)
               aaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTA‑AGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑Ttgg                                                                                                                                       <  5:1642637/151‑1 (MQ=255)
                 aaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑Ttggt                                                                                                                                      <  1:302325/151‑1 (MQ=255)
                  aaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGCGCTATAGCCCCACGATGCGTGTACGTACCAAGTTTGCTGGGTGCT‑AAAT‑Ttggtg                                                                                                                                     >  6:714730/1‑151 (MQ=255)
                  aaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGAAGAGTTGACTTACCAATTTACCTGGGGGCA‑AAAT‑Ttggtg                                                                                                                                     >  2:796225/1‑151 (MQ=18)
                   aCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑Ttggtgg                                                                                                                                    <  4:1465033/151‑1 (MQ=255)
                    cccccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGa                                                                                                                                   <  5:1727760/151‑1 (MQ=255)
                     ccccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAg                                                                                                                                  <  2:95249/151‑1 (MQ=255)
                      cccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGCGATGGGTTTACTTACCAAGTTTGCGGGGTGCT‑AAAA‑TTGCTGGAGc                                                                                                                                 >  5:1187700/1‑151 (MQ=21)
                      cccGCTTTGCAGCGAGGGTTGGAAA‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCt                                                                                                                                <  4:10659/151‑1 (MQ=255)
                        cGCTTTGCATCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTa                                                                                                                               <  8:1352313/151‑1 (MQ=255)
                         gCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTaa                                                                                                                              <  7:498465/151‑1 (MQ=255)
                         gCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTaa                                                                                                                              <  3:838632/151‑1 (MQ=255)
                         gCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTaa                                                                                                                              <  1:1007654/151‑1 (MQ=255)
                           tttGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGAGCTCTCCCAGCTGAGCTATAGCCCCACGATGACTTTAATTACCAAGTTTCCTGGGTCCA‑AAAT‑TTGGTGGAGCTa                                                                                                                               >  4:183426/1‑148 (MQ=21)
                            ttGCAGCGAGTGTTGGAAATTTGGTTGAGCTAATCGGGATCGAACCGCTGACCTCTTGCATGCCATTCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAg                                                                                                                             <  6:1485775/149‑1 (MQ=255)
                             tGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCg                                                                                                                           <  3:720454/150‑1 (MQ=255)
                              gCAGCGAGGGTTGGAAATTTGGTTGAGCTAAGCTGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGCGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCggg                                                                                                                         <  7:1368978/151‑1 (MQ=255)
                              gCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCgg                                                                                                                          <  3:250729/150‑1 (MQ=255)
                                 gCGAGGGTTGTTAATTTGTTGCATCTAAGGTTGATCGAACCTCTGAGCTCTTGCATGCCATGATAGCGTTCTCCAACCTGAGCTATATCCACACTATTATTTTACGTACCATGTTTGCTGGGTTGA‑AAAT‑TTGGTGGAGCTAAGCGGGATc                                                                                                                      <  4:1642042/151‑1 (MQ=255)
                                  cGAGGGTTGTAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTCCATGCCATTCAAGCGCTCTCCCACCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCg                                                                                                                     <  1:1374171/151‑1 (MQ=255)
                                    aGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCCGAGGCGTATACGTACCAAGTTACGTGGGTCAT‑AAAT‑TTCGTTGAGcaacgctggctcca                                                                                                                    >  3:1061279/1‑137 (MQ=17)
                                    aGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGATTACGTACCAAGTTTGCTGGGTGCT‑AAAT‑TTGGTGGAGCTACGCTGGATCGa                                                                                                                    >  3:907223/1‑150 (MQ=255)
                                     gggTTGGAAATTTGGTGGAGCTAAGCGGCATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCAAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGGA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGaa                                                                                                                   <  5:891194/150‑1 (MQ=255)
                                      ggttttaaaTTTGTTGGAGCTAAGGGTGATCGAACCGCGGACCTCTAGCATGCCATGCTAGCGATCTCCCACCTGTGCAATAGCCCCACTATGCGTTTACGTACCTAGTATGCTGGGTGGA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAAcc                                                                                                                 <  6:518542/145‑1 (MQ=255)
                                      ggTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCCGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGAGGGATCGAAcc                                                                                                                 >  5:1606134/1‑151 (MQ=255)
                                      ggTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCCCACGACTTTAAGGTCCAAGTTTCGTGCGT‑CA‑ACATATTCGTGGAGCTaccccggatggcaac                                                                                                                 >  5:1247955/1‑137 (MQ=2)
                                      ggTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACCATGAAAATAATTACCAAGTTAGCTGCGTCAA‑AAAT‑TTAGTGCAGCTaaaatgtatcaaacc                                                                                                                 >  2:529261/1‑138 (MQ=14)
                                        ttGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGAAAGCGCTCTCCCAGCTGAGCTATAGCCCCAACATGCTTTTAGTTACCACGATTCCTGCG‑GCATAAAT‑TAGGTGCAGCTACAATGGATCGAACCg                                                                                                                >  5:258325/1‑150 (MQ=9)
                                          ggAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCCCAAGCGTTGAGGGACCAAGTTACCGGGGGGCA‑CAAT‑TTCGTGGAGCGACGCGGGCTCGACCCGcac                                                                                                             >  8:1730979/1‑149 (MQ=14)
                                           gacaTTTGGTGGAGCTAAGCGGGATCGACCCGCGGTCCTCTTGCCGGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGa                                                                                                            <  5:1771056/148‑1 (MQ=25)
                                           gAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCGGAGGGGTATAGGTACCATGTGTGCGGCGGGCT‑AAAT‑TTAGTTGAGCTAAGAGGGCTCGaccgcctg                                                                                                             >  5:1321534/1‑143 (MQ=14)
                                            aaaTTTTTTGGATCTAATCGGGATAGAACCGCTGACCTCTTGCATGCCATGAAAGCGCTCTACCAGATGAGCTATAGCCCCACCATGCGTTTACGTCCCAAGTTTGCTGGGTTCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGAc                                                                                                           <  5:723519/151‑1 (MQ=25)
                                               tttGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTAAGTACCACGTTTCCTGGGGGCT‑AAAT‑TTGGTGGCGCTCAGCGGGGTCGACCCGCTGACctc                                                                                                        >  8:73946/1‑151 (MQ=18)
                                                ttGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCACAAGCTAATCATTACCAATTTACCTGCGTGCT‑AAAT‑TTAGTTGAGCTAATCGGGATCGAACCGATGaacac                                                                                                        >  4:75367/1‑146 (MQ=9)
                                                ttGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAGCAGGGGTTTAGGTTCCAGGTTTCGGGGGTGCT‑AAAT‑TTGGTGGAGCTACGCGGGATCGACCCGATTACctct                                                                                                       >  3:1580554/1‑151 (MQ=17)
                                                 tggttgATCTAATCTTGATCTCACCGCTCACCTCTACCAAGGCATGAAACCGCTCTCCCAGCTGAGCAATACCCACACTCTCCCATTACGCTCCAAGCATCCTGGGTTCT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACctct                                                                                                       <  4:58011/150‑1 (MQ=9)
                                                   gtggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCCGAGGGGTTGAGGTTCGAGGTTAGGTGCGGGCT‑AAAT‑TTCGTGGAGCGAGGAGGGCTCGAccccatgagctatagc                                                                                                    >  5:1431103/1‑138 (MQ=2)
                                                     ggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGca                                                                                                   <  3:295581/150‑1 (MQ=255)
                                                      caGCTAAGGCGGCTCGAACCGCGGAGCTCTTCCATGGCAGGCAAGCGCTCTCCCAGCTGAGCTAAACCCCCAGTCTGCGATTACGTCCCTAGTTTGGTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatg                                                                                                 <  1:1477893/150‑1 (MQ=21)
                                                          gaatcGGGATGTAACCCGTGACCTCTTTCCTGCCATTATAGCGCTGTCCCACATGAGCTATAGCCCCACTATGCGTTTACGTACCTTGTTTGCTGGGTGGA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccat                                                                                             <  7:1684311/147‑1 (MQ=21)
                                                            atcgtgctCGAACCGCTCAGCTCTTGCAGGCCCTGAAAGCGCTCTCCCAGCGCAGCTATATCCCCACTATGCCTTTACGTACCAAGTTTGGTGGGTTGA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccatgc                                                                                           <  3:1306401/144‑1 (MQ=21)
                                                            aggcggATCTAACCTCTGTCCTCTTTCATGGCATGCAAGCGTTCTCCCACCTGAGCTATATCCCCACGATTCTTTTACGTTCCTAGTTTGGTGGGTTCT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccatgc                                                                                           <  4:1305819/147‑1 (MQ=21)
                                                              cGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCCGGAGGGGTATAGGTACCAAGTTTCCTGGGGCCA‑AAAA‑TTGGTGGAGCTAACCGGGATCGAACCGATTAACTCTTGCCTGCCATGCa                                                                                          >  8:456410/1‑150 (MQ=18)
                                                                ggctCGAACCTGTGAGCTCTTGCATGGAATTAAAGCGCTCTACCATATGAGCTATATCCACACTATTCGTTTACGTTCCAAGTTTGCTGGGTTCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc                                                                                       <  2:3140/148‑1 (MQ=18)
                                                                ggATCGAACCGCTGACCTCTTTCATGACATTCAAGCGCTCTCCCATCTGAGCTATAGCCAGACGATTCGTTTACGTACCAAGTTTGCTGGGTTCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc                                                                                       <  6:998645/151‑1 (MQ=37)
                                                                   tCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAAGATGCGTTTAAGTACCAAGTTAGCGGGGTGCA‑AAAT‑TTGGTTGAGCTAAGCTGGGTCGACCCGCTGAACTCTTGCCTGCGATGCAAgcggg                                                                                    >  7:994947/1‑149 (MQ=25)
                                                                    agcacaggtGACCTCATACATGCCATCCAACCTCGCTACCAGCTGCGCTATACCCACACCAACCCAATACGCACCAAGTATGCTGGGTGGA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctc                                                                                   <  1:1331129/143‑1 (MQ=12)
                                                                      aaCCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTACGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCtctc                                                                                 >  8:635216/1‑151 (MQ=255)
                                                                      aaCCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGAGTTTAGTTACCAAGTTACCTGGGGGCA‑AAAT‑TTGGTGCAGCTAAGAGGGGTCGACCCCCAGACCTCAAGAATGCCTTCCAAGCGatca                                                                                  >  5:1556403/1‑149 (MQ=18)
                                                                        ccGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGGGAGGGGTTGCGGTTCCACGTGTCGTGGGGGCT‑AAAA‑TTCGTGGAGCTACCAGGGCTCGACGCGCTGAACTATTGTCTGGGATGCAAGGGGGCTccc                                                                               >  3:674843/1‑151 (MQ=2)
                                                                            ggACCTCTTGCAAGGCATGCAAGCGCTCTCCCAGCGCAGCTATAGCCCCACGATGCGTTAACGTACCAAGGTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt                                                                           <  1:62571/150‑1 (MQ=25)
                                                                               ccTCTTGCAGGCCATGCAAGCGCTCTCCCACCGCAGCTATAGCCCCACGATCCGTTGACGTCCCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAg                                                                        <  3:463007/151‑1 (MQ=25)
                                                                                ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCAAAGCGGGATCGAACCGCTGACCGCTTGCATGCCATGCAAGCGCGCTCACACCGGAGc                                                                       >  2:1220650/1‑151 (MQ=37)
                                                                                    ttcatggaattcAAGCGTTCTACCAGCTCAGCTATATCCACACTATTAGTTTACGTTCCTAGTTTGGTGGGTTCT‑ATAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta                                                                     <  7:1194535/138‑1 (MQ=17)
                                                                                    tgcccgccatgcAAGCGCTCTACCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCATGCTGGGTGGGTGCT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGACCCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta                                                                     <  1:934378/144‑1 (MQ=21)
                                                                                      catgccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGc                                                                 <  5:399320/151‑1 (MQ=255)
                                                                                        tgccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCCGGAGGCGTTTAGGTACGAGTTTTCGTGGGGCCA‑AAAA‑TTGGTTGAGCTAGGAGGGGTCGACCCGATGAGCGAAGGCATGCCATCCATGCGGGCTACCTGAGGAGCTAGAGca                                                                >  5:1142715/1‑149 (MQ=9)
                                                                                           catgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGCGATGGGTATCGGTACGAGGTTTGGGGGGGGCT‑AAAT‑TTGGTGGCGCGACGAGGGCTCGACCCGCTGACCGCTTGTCTGGGATGAAAGCCCGCTCCCAGCGGCGCTATAGcacctt                                                            >  1:1054253/1‑149 (MQ=255)
                                                                                           catgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGCGAGGCGTTTACGTACCAAGTTTCGTGGGTGCA‑AATT‑TTGGTTGAGCTAAGAGGGGTCGAAGCGCTGAACTCTTGCATGGCATGCAACCGCTCTACAACATGCGATATAGccagtt                                                            >  1:762735/1‑147 (MQ=255)
                                                                                            atgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCCGGATGGGTTTAGGTACCAAGTTTGCGGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCCTGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCACGTc                                                           >  5:51239/1‑151 (MQ=21)
                                                                                                 aGCGCTCTCCAAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGGTGGGTGCA‑AAAT‑TTGGTGGAGGTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTa                                                      <  6:607309/151‑1 (MQ=255)
                                                                                                  gcgcTCTCCCAGCTGAGCTATAGCCCCCGGATGCGTTTACGTACCAAGTTTCCTGCGTGAT‑AAAT‑TTGGTGGAGCAAAGAGGGGTCGAACCGATGAGCTCTTGCATGCGATGCAAGCGGTATACCAGCTGAGCTATAGCCAGGTCACGTa                                                      >  5:744707/1‑150 (MQ=255)
                                                                                                     ctctCCCAGCTGAGCTATAGCCCCAGGAGGAGTTTACGTACCAAGTTTCCTGCGTGCA‑AAAT‑TTGGTTGAGCTAAGATGGATCGAACCGCAGACCTCTTGCCTGCCATGCAAGCGCTCTCCCAGATGAGCTATACCCCCGTCACCTAAAGc                                                  >  3:1379411/1‑151 (MQ=21)
                                                                                                       ctcCCAGCTGAGCTATAGCCCCACGATGGGTTTACGTACCAAGTTTGCTGGGGGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCGATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCAGTCACGTAAAGCtt                                                >  8:659480/1‑151 (MQ=255)
                                                                                                           cAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCg                                            >  5:80462/1‑151 (MQ=255)
                                                                                                            agaggAGCTATAGCCACACGATTAGTATACGTTCGTTGTTTGGTGGGTTGT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGa                                           <  7:1286594/147‑1 (MQ=17)
                                                                                                             gCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCg                                            >  6:848889/1‑149 (MQ=255)
                                                                                                              cTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGCCCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGt                                         >  2:1019163/1‑151 (MQ=255)
                                                                                                              cTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGt                                         >  8:290929/1‑151 (MQ=255)
                                                                                                                caGCTATAGCCCCACGAAGCGTTTCCGTACCATGCTTGCTGGGTTGA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGt                                         <  3:1659833/148‑1 (MQ=18)
                                                                                                                      taGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACgggg                                 >  3:1469887/1‑150 (MQ=255)
                                                                                                                      taGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGggc                                 >  6:892718/1‑151 (MQ=255)
                                                                                                                      aatgcagaggattattttACGTTCTTAGTTTGGTGGGTTGT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCTGTCACGTAAAGCTTGTCGAGTTGACGggc                                 <  2:1599046/136‑1 (MQ=17)
                                                                                                                      aatccagagtatccttttACGTTCGAAGCTTGGTGGGTTGA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGggc                                 <  6:1091146/136‑1 (MQ=17)
                                                                                                                      aacccacACCATGCCTTGACGTACCTAGGTTCCTGGGTGGT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGggc                                 <  1:175618/145‑1 (MQ=18)
                                                                                                                       aggcccACGATGAGTTTACGAACCTAGGTTCGTGGGTTCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGggcg                                <  8:826400/148‑1 (MQ=18)
                                                                                                                           ccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGcat                            >  6:423430/1‑151 (MQ=255)
                                                                                                                            cACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGcatc                           >  5:429625/1‑151 (MQ=255)
                                                                                                                             aCGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGcatc                           >  2:1155140/1‑150 (MQ=255)
                                                                                                                               gATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCACGTAACGTAAACCTACTCGAGTTGaatggagtcataat                         >  6:1204748/1‑137 (MQ=18)
                                                                                                                               ccagCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCata                        <  8:1676868/148‑1 (MQ=255)
                                                                                                                                aTGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCata                        >  7:21382/1‑150 (MQ=255)
                                                                                                                                 tGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATg                      >  3:1692873/1‑151 (MQ=255)
                                                                                                                                  ccttttACGTACCTAGTTTGGTGGGTTGT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATg                      <  7:653235/147‑1 (MQ=21)
                                                                                                                                    gTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAAt                   >  6:342885/1‑151 (MQ=255)
                                                                                                                                     tttACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAAtt                  >  4:1243387/1‑151 (MQ=255)
                                                                                                                                      ttACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTc                 >  5:468081/1‑151 (MQ=255)
                                                                                                                                       tACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGCCCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTcc                >  6:1683061/1‑151 (MQ=255)
                                                                                                                                       tACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTcc                >  1:1192197/1‑151 (MQ=255)
                                                                                                                                         cGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGc              >  8:1083143/1‑151 (MQ=255)
                                                                                                                                              cAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGa          >  1:390814/1‑150 (MQ=255)
                                                                                                                                               ttgcatGCTGGGTTCT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCg           <  4:989784/143‑1 (MQ=255)
                                                                                                                                               ttgTTTGGTGGGTTGT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGa          <  6:1737476/147‑1 (MQ=255)
                                                                                                                                               aaGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAAt        >  3:39765/1‑151 (MQ=255)
                                                                                                                                                aGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAAtg       >  2:1349110/1‑151 (MQ=255)
                                                                                                                                                 gcttGGTGGGTTCT‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAAtg       <  7:832164/148‑1 (MQ=255)
                                                                                                                                                    tGCTCGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTCAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGATCACGGGCGGCATCATATCAAATCCACCCGAATGtctc   >  4:314367/1‑151 (MQ=21)
                                                                                                                                                      |                                                                                                                                                       
CAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCA‑AAAT‑TTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAATGTGTCA  >  NC_000913/2517992‑2518291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑