Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9940GT24.1% 264.2 / 20.7 199intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (13/110);  new base T (0/39);  total (15/184)
Fisher's exact test for biased strand distribution p-value = 3.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

GAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTT  >  NC_000913/3269845‑3270129
                                                                                                                                                     |                                                                                                                                       
 aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                                       <  7:1481228/151‑1 (MQ=255)
 aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                                       <  6:1638755/151‑1 (MQ=255)
  aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                                                      <  5:1128411/151‑1 (MQ=255)
  aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                                                      <  6:435443/151‑1 (MQ=255)
     ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                                                                                                                                   <  4:1381291/151‑1 (MQ=255)
     ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                                                                                                                                   <  4:1632116/151‑1 (MQ=255)
      cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                  <  1:743956/151‑1 (MQ=255)
      cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                  <  3:333532/151‑1 (MQ=255)
       gccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                                                 <  8:657927/151‑1 (MQ=255)
       gccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                                                 <  2:1380086/151‑1 (MQ=255)
       gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTAGTGGAAAGTCATTCATGTTTGGCCACCGTCAAAAACAAAAAAACGCCGAAGAACGGTTTTAAGTTAAACACGTACAACTTAc                                                                                                                                 >  8:715128/1‑151 (MQ=2)
        ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                <  4:796784/151‑1 (MQ=255)
        ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                <  4:980790/151‑1 (MQ=255)
        ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGagg                                                                                                                                >  2:629092/1‑149 (MQ=255)
         cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                                               <  3:77415/151‑1 (MQ=255)
         cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                                               <  1:653411/151‑1 (MQ=255)
          ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                                                              <  2:83258/151‑1 (MQ=255)
          ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                                                              <  2:1185699/151‑1 (MQ=255)
              cGGTTTATTACGTACATCATGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAGCGTCATTGATCTGTGGTGCCCTCCACAACGAAAAAACCGCCGAAGAGGCGTTTTTCGTATAGCACTTCAAACTGACCGCTa                                                                                                                            >  8:1647587/1‑149 (MQ=9)
               ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                                         <  4:496951/151‑1 (MQ=255)
               ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                                         <  5:761695/151‑1 (MQ=255)
                gTTTATTACGTACATCATGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                                        <  7:953781/151‑1 (MQ=255)
                gTTTATTACGTACATCAGGTAAAACTGACCGATAAGGCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                                        <  1:1484551/151‑1 (MQ=255)
                 tttATTTCGTAGAACATGTTAAAATGAGCGTTAATAAGCTTTCTTTTTTGTATAGTGTCGTGGACAGTGTTTCTTCTTTCTCGCACTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                                        <  4:1307797/150‑1 (MQ=2)
                  ttattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCg                                                                                                                       <  5:395645/150‑1 (MQ=255)
                    aataCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctt                                                                                                                    <  1:1317317/149‑1 (MQ=255)
                       aCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGCTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                                                  <  2:1467552/150‑1 (MQ=255)
                         gTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                                               <  8:272723/151‑1 (MQ=255)
                           acttCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTCTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                             <  5:489218/148‑1 (MQ=37)
                           aCATCATTTAACACTTACCGATAAGCAGCTTTCTTTTGCCTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                             <  4:925685/151‑1 (MQ=11)
                           aCATCATGTAAAACTGACCGATAAGCAGCTTTCTTTTGGGAATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                             <  8:298806/151‑1 (MQ=255)
                               attttAAAACTGAGCGATATGCTGTTTTCTTTTGTTTATTGTGTCGTGGAATGTCTTTCTTATTTCTGCCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                                                                          <  6:973722/146‑1 (MQ=11)
                               aatgTAACACTTACCGATAAGCAGCTTTCTTTTGCTTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                         <  3:915864/148‑1 (MQ=11)
                               aaGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTATCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                         <  8:1342023/150‑1 (MQ=25)
                                    aaaaCTGACCGATCAGCGTGTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCAAAAAGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgt                                                                                                     <  5:887204/150‑1 (MQ=11)
                                      aactttccGATAAGGCGCTTTCTTTTGTGTATAGTGTCGTGGAAAGTGTTTCTTCTTTCTGCCCCTCAAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtgt                                                                                                   <  6:896642/144‑1 (MQ=11)
                                          gtccGATCAGCGGGTTTCTTTTGTTTCTAGTGTCGTCGAAAGGGTTTCCTATTTATGCCCCTCCAAAAGTAAAAACCCGCCGTATCGGGTTTTTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                               <  7:308687/148‑1 (MQ=11)
                                          gACCGATAAGCGGCTTTCTTTTGTTTATATTGTCGTGGACAGTCTTTGATATTTCTGCCACTCAAAAATTATAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                               <  6:1742507/150‑1 (MQ=11)
                                            ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTTATCTTTCTGCCCCGCCAAAACTAAAAACCCCCCGAACCGGGGTTTTAACTAAAAAAGGTGAAAAAGAACGATAAGCCGCGTTCTTTTtggggttgtggggggggg                                                                                            >  5:51655/1‑136 (MQ=2)
                                              gATAAGGCGCATTCTTTTGCCTATAGTGTCGTGGAAAGTCATACCTATTTCGCCCCCTCAAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAg                                                                                          <  6:1174794/151‑1 (MQ=11)
                                                 aattggCTTTCTTTTTGTTCTAGTCTCGCGGCAAGGCCTTCTTCTATCTGCCACTCAAAAAGGACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtca                                                                                       <  3:251634/146‑1 (MQ=11)
                                                 aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTGATCTTTGTGCCCAAACAAAAAAAAAAACCAGCCGAATAGCGTTTTTAAGTAAAAAAGGTGAAAATCACCGATAAGCCGGGTTGAGTTGGGTATAgtgtgttgtaaaggg                                                                                        >  7:335005/1‑139 (MQ=2)
                                                  aGCCGCTTTCATTTGGGTAGAGTGTCGCGGACAGGCATTCATCTTTCTGCCACTCCACAAGTACAAACCCGCCGAACCGGTTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcat                                                                                      <  1:1301115/151‑1 (MQ=11)
                                                      gCTTTCTTTTGTGTATCGTGTCGGGGAAAGTGTTTTCTCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattcat                                                                                  <  7:716634/151‑1 (MQ=11)
                                                       ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTGGCACCCACCAAAACTAAAAACCCCCCGAAGCGCGTTTTTACGTAAAACAGGGGAAACTGACCCATACCCCGCGTTGTGTCGGGGAGAGTGTCGttggtaggccatcaa                                                                                  >  1:327381/1‑136 (MQ=2)
                                                         ttctttTGCGTATAGTGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATc                                                                                 <  5:1505697/149‑1 (MQ=25)
                                                          tctttTTGGTATAGTCTCGTGGACAGTCATTCCTCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCtt                                                                               <  5:1528706/150‑1 (MQ=255)
                                                          tctttAGGGTATAGTGTCGTCGACAGTCTTTCCTCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCtt                                                                               <  5:858006/150‑1 (MQ=255)
                                                             ttttttgcttttgtCGTCGAAAGTCTTTTTTATATATGCCACTCCAAAATTACAAACCCGCCGAATCGGGTTATTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTc                                                                             <  6:1529161/138‑1 (MQ=11)
                                                             ttttGGTGTAGTGTCGCGGACAGGGATATATCTATCTGCCACTCCAAAATGACAAACCCGCCGCACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCt                                                                            <  1:1038423/150‑1 (MQ=11)
                                                             tttGGGGATAGTGTCGTGGACAGGCATTCATCTTTCTCCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCt                                                                            <  5:43856/150‑1 (MQ=255)
                                                             ttagagTATAGTGACGTGGACAGTGATTCCTCTTTCTGCCCCTCCAAAAGTACAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCttt                                                                              <  1:373015/143‑1 (MQ=11)
                                                              tttccttttgtctCGTGGAAAGTCTTTTCTCTTTCTGCCACTCCAAAAGTTAAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTg                                                                           <  6:31220/138‑1 (MQ=11)
                                                              gtGGGTATAGTGTCGTGGCCAGGGCTTGATCTTTCTGCCCCTCCACAAGTACAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTg                                                                           <  8:941088/149‑1 (MQ=11)
                                                                 tgTATCGTGTCGGGGACAGTGTTACTTCTTTCTGCCCCTCAAAAAGTACAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGccc                                                                        <  6:1780757/149‑1 (MQ=11)
                                                                     tattctcgcggaatgTCTTTCTTCTTTCTCGCCCTCAAAAAGTATAAACCCGCCGGATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                    <  6:822921/136‑1 (MQ=11)
                                                                      cgtctcggcgACAGCCAAACATATTACTGCCACTCAAAAATCAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaa                                                                  <  1:1612994/142‑1 (MQ=11)
                                                                         gtCGCGGACAGTCATTCATCTTTCTGCCCCTCCTTTTTTTTAAATGATACGCAGAGGTTTTTTATGTAAACATGGTTATACTGTCTTTTAATACTCTTTGTTTTTGGTATATTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaa                                                                 <  4:279366/149‑1 (MQ=2)
                                                                           cGTGGACAGTCCGTCATCTTTCTGGCACTCCAAAAGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGc                                                              <  1:1756563/150‑1 (MQ=255)
                                                                               gtcagtcttggctgtttCTGACCCTCAAAAATCACAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                         <  5:618059/137‑1 (MQ=11)
                                                                               gACAGGCGTTCATCTTTCTCCCACTCCAAAAGTACAAACCCGCCGTATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                         <  7:478779/151‑1 (MQ=11)
                                                                                aCAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGAACCTATCATATGCGTATAGTGTAATGGAGACTAATACATATTTCTTCACCGTCAAAACCaaaaa                                                         >  7:899671/1‑150 (MQ=11)
                                                                                 attcgatttatatttatGGCACTCAAAAATTTTAAACCCGCCGTATCTGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                       <  7:1276835/146‑1 (MQ=11)
                                                                                   gTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                     <  5:1724104/151‑1 (MQ=25)
                                                                                     ccttcctCTATCTGCCACTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                   <  5:1306817/149‑1 (MQ=11)
                                                                                       ttaatctatctcgcacTCAAAAAGTATAAACCCGCCGTATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                  <  6:114358/135‑1 (MQ=11)
                                                                                       cacctCTTACTCCCCCTCCAAAATGACAAACCCGCCGCAGCGGTTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                   <  3:1493774/145‑1 (MQ=11)
                                                                                       aatatatttCTCGCCATCAAAAAGTAAAAACCCGCCGAAGCGGTTTTTTTCGTATATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                 <  7:1329038/145‑1 (MQ=11)
                                                                                         cctCTTTCTGGCCCTCAAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                               <  5:1199820/149‑1 (MQ=11)
                                                                                         actCTTTCTGCCACTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                <  5:309052/148‑1 (MQ=16)
                                                                                          atatttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                              <  3:848512/148‑1 (MQ=25)
                                                                                          aaatttCTGGCACTCCAAAATTTAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                              <  6:1344161/148‑1 (MQ=11)
                                                                                            ttttgttctacTCCAAAATGACAAACCCGCCGTATCGGGTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                             <  6:1280115/140‑1 (MQ=11)
                                                                                            atttatcgcccTCCAAAATTATAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                            <  4:1351865/143‑1 (MQ=11)
                                                                                               actcccacTCCAAAAGCTAAAACCCGCCGAACCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                         <  3:1226245/144‑1 (MQ=11)
                                                                                                 gggcactcaacaaGTAGAAACCCGCCGCAGCGGGTTATTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                       <  5:1037640/140‑1 (MQ=11)
                                                                                                  cccacTCCAAAAGCAAAAACCCGCCGCACCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                       <  8:373433/146‑1 (MQ=11)
                                                                                                   ccccTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                     <  5:375644/151‑1 (MQ=25)
                                                                                                     ccTCAAAAAGTTAAAACCCGCCGAATCTTTTTTTTACGTAAATCAGGTGAATCTGACCGATAAGCCTCTTTCTTTTGGGTATAGTGTCGTGGAGAGTCATTCATGTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                   <  4:1218894/151‑1 (MQ=11)
                                                                                                        caacaattataaaCCCGCCGCACCGGTTTTTTACGTAAAACAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                <  3:1348107/141‑1 (MQ=11)
                                                                                                            aaggacaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                            <  5:1640238/145‑1 (MQ=255)
                                                                                                             atttaaaaCCCGCCTAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                           <  6:484848/147‑1 (MQ=255)
                                                                                                                acaaaCCCGCCGGACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                        <  5:546650/149‑1 (MQ=255)
                                                                                                                 caaaCCCGCCGCATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                         <  7:1617447/148‑1 (MQ=11)
                                                                                                                 aaaaCCCGCCGTATCGGTTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                       <  2:1006525/151‑1 (MQ=255)
                                                                                                                 aaaaCCCGCCGCAGCGGTTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                       <  8:1231686/151‑1 (MQ=255)
                                                                                                                  atgcactgcctacggggTATATACGTAACTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa                      <  1:1273810/137‑1 (MQ=11)
                                                                                                                   aaCCCGCCGAAGCGGGTTTTTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                     <  5:250897/151‑1 (MQ=25)
                                                                                                                     cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                   <  3:877446/151‑1 (MQ=25)
                                                                                                                     accgccgTATCTGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                   <  4:502668/150‑1 (MQ=255)
                                                                                                                      ccgccgCAGCGGTTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg                  <  5:1756950/151‑1 (MQ=255)
                                                                                                                      ccgccgAAGCGGGTTTTTACTTAAAAAATGTTACACTGACCTATCAGCCGCTTTCTTTTGTGTATAGTGTCTTGGACATTCATTCTTTTTTGATCTCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg                  <  7:1184368/151‑1 (MQ=11)
                                                                                                                       cgccgcAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                 <  3:199446/151‑1 (MQ=25)
                                                                                                                               cGGGTTATTACGTAAATCAGGTAAAACTGACCGATAACCCGCTTTCTTTTGTGTATAGTGTCTTCTACAGTGTTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg         <  8:84391/151‑1 (MQ=11)
                                                                                                                                 gttttttACGTAATATAGGTGAACCTGACGGTTATGCGTTTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg         <  6:574962/147‑1 (MQ=11)
                                                                                                                                  gTTATTACGTAAATCATGTAAATCTGTCCGATAAGACGCTTTCTTTTGTGTATAGTGTCGTGGACAGTGATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc       <  3:489550/150‑1 (MQ=11)
                                                                                                                                   tttttACGTAAAACAGGTGAAACTGACCGATAAGCCGATTTCTTTTGGGTATAGTGTCTTGGAAAGTAATATATCATTGTTCCCCAAAAAAAAAAAAAAAAAACCGAAGCGGGGTTTTTCGTAAAACAGGTGAAACAGAGCGGTAAGGCgg     >  6:632339/1‑150 (MQ=2)
                                                                                                                                   ttattACGTAAAACAGGTTAAACTTACCGATAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTGATTCATATATCTCCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg      <  5:1094452/147‑1 (MQ=11)
                                                                                                                                       tacttaaataatgTGAAACTCACGGATAAGCCTCTTGATTTTTGGTATAGTGTCGTGGAAAGTCATTCCTATTTATGCCACTCAAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGcttt  <  5:359879/138‑1 (MQ=11)
                                                                                                                                              aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                           <  8:1588761/118‑1 (MQ=37)
                                                                                                                                                     |                                                                                                                                       
GAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTT  >  NC_000913/3269845‑3270129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑