BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,151	0	A	C	24.0%	212.2 / 28.1	221	intergenic (+549/+474)	yhaC/garK	pentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand): ref base A (100/58); new base C (50/0); total (162/59)
Fisher's exact test for biased strand distribution p-value = 8.57e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

ATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270005‑3270301

cccccccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCTTTTTTTTTATGATCCTTAATCAAAATCGATCTCAAGAGGGTTTTTAAGTATTTCAGTTGAATATGATCTTTATTTCTCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                                         <  6:529248/144‑1 (MQ=2)

 tAAGCAGCTGGCTTTTTCTTATAGTGTCGAGGAATGTCTTTCCTATATATGCCACTCAAAAATCATAAACCCGCCGTACCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt                                                                                                                                                       <  6:267551/150‑1 (MQ=2)

        ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTGTCCCCCTCCAAAACCAAAAACCCGCCGAATCCGGTTTTTAAGTAAACCAGGTGAAAATGACCGATAACCCGGGTTCTTTAGGGGATAGCGTCGGGGCCAGtcaataaa                                                                                                                                                >  1:763893/1‑145 (MQ=11)

          ttctttTGGGTATAGTGTCGTGGCCAGGCATTGATCTTTCTGCCCCACCAAAATCAAAAAACCCCCGCAGCCGGGGTTTTCGTAAACCAGGTGAAACTGACCGATAACCCGGGTTATTTTGGGTTTAGCGTCGTGGTCacgccatccaatt                                                                                                                                             >  5:94026/1‑139 (MQ=2)

                          gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTTATAGTTGTGTGCCTCCaaaa                                                                                                                              >  5:1367719/1‑150 (MQ=11)

                                 aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGGATAGCGTCGAGGCCAGTCAAGCATAGTATAGCACCTCCaaaaacaaaaac                                                                                                                      >  8:553605/1‑151 (MQ=11)

                                   aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTCGTGGAGAGTGATGGATGGTTTGGCCCCAGCAAAAGCAAAAAccc                                                                                                                    >  4:186985/1‑151 (MQ=11)

                                     tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTAATAGTTGTGCCCCGCCAAAAACAAAAAacccc                                                                                                                  >  5:706754/1‑149 (MQ=11)

                                     tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGAGGCTAGGCATTCATGGTTGCGCGCCTCCAAAAGCAAAAACccgc                                                                                                                  >  1:1048108/1‑151 (MQ=11)

                                     caacacctcgctCTTCCCATCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACATTTTAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                                                                                  <  6:505062/140‑1 (MQ=11)

                                      gtttgatCTTTCTATCACTCCAAAATCTTAAACCCGCCGTATCGGGTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                 <  8:1655197/146‑1 (MQ=11)

                                        ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGCAACTAAATCAAAATTGTGCCCCACCAAAAGCAAAAACccgccgc                                                                                                               >  1:878494/1‑150 (MQ=11)

                                          gctgccgctcacccTCAACAAGCTAAAACCCGCCGGAGCGGGTTATTACGTAAAGCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAg                                                                                                            <  3:1771558/140‑1 (MQ=11)

                                           atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGGGTAGAGCGACGGGGCAAGGCCTGCATGAGGGAGCGCCAACAAAAACAAAAACCCGCCG‑AAgg                                                                                                           >  7:183832/1‑150 (MQ=2)

                                            tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGCGAGTCAATCATGTTTTGGCGCCGGCAAAACCAAAAACccgggtaagcg                                                                                                           >  1:206012/1‑143 (MQ=11)

                                              tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGAGAGCGTCGAGGCGAGTCATTCATCTTTATGCGCCACCAAAACCAAAAACCCGCAG‑aaccccg                                                                                                        >  8:1699890/1‑146 (MQ=11)

                                              tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTGGGGTAAAGCGTCGTGGCCAGTCCTTCAAAGTTAGCCCCCCACAAAAGCAAAAACCCCCCG‑CAG‑Cgcg                                                                                                       >  3:538067/1‑149 (MQ=11)

                                                tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGGGGAGAGCGTGGGGGCGAGGCATTCAAACGTGGGCCCCGGCAACACCAAAAACCCGCCC‑ACG‑CG‑CGtg                                                                                                    >  8:1033833/1‑150 (MQ=0)

                                                 cTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTATTCTAAAGTCAATAAAATTTACACACCAACAAAAACAAAAACCCGCCG‑AAgaacgttt                                                                                                     >  4:1312080/1‑144 (MQ=2)

                                                   gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCCTCGTGGAAAGTCAATCAAAATTATGCACCAACAAAAACAAAAACccgcatcagaacgattat                                                                                                    >  6:1352931/1‑137 (MQ=11)

                                                     cccTCCACAATCAAAAACCCGCCGGACCGGGTTTTTACGTAAACCATGTGAAACTGACCGATAAGCCGCATTCTTTTGGGTATAGCGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTAc                                                                                               <  5:1272125/151‑1 (MQ=11)

                                                     cccTCAACAATCAGAACCGCGCCGCAGCGGGTTTTTACGTACACCAGGACAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTAc                                                                                               <  6:1130315/151‑1 (MQ=11)

                                                           aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTAAGCGCCTTCAAAAACAAAAACCCGCCG‑AAG‑CG‑CGTTGTTACGTAAAt                                                                                         >  1:850981/1‑151 (MQ=11)

                                                           aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTAGGCCCCAGCAAAAGCAAAAACCCGCCG‑AAG‑AC‑CGGTGTTACGTaaa                                                                                          >  2:1521491/1‑150 (MQ=11)

                                                              atcagaaaCCCGCCGCACCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAg                                                                                      <  4:1040118/146‑1 (MQ=11)

                                                               cctgaaaCCCGCCGTACCGGGTTATTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAgg                                                                                     <  3:210661/147‑1 (MQ=11)

                                                                cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCCTTCCTGGTGCTGCCCGGGCAAAAGCAAAAACCCGCCG‑CAG‑CGCGGTGTTTaggaaatgacgg                                                                                     >  3:1505931/1‑140 (MQ=11)

                                                                 aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCCGGCCTTCAAGTTTGGGCCCCGGCAAAAGCAAAAACCCGCCG‑GAG‑GG‑GGGTTTTTCGTAAATCAgggg                                                                                   >  1:1422318/1‑149 (MQ=11)

                                                                 aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATAAAATTTAAGCACCAACAAAAACAAAAAACCGCCG‑AAG‑CG‑CGTTTTTAACTAAATCAAGTg                                                                                   >  4:1462794/1‑151 (MQ=11)

                                                                    aaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAGGTTGTGCCCCAGCAAAACCAAAAACCCGCCG‑CAG‑CG‑GGGGTTTAAGTTAATCAGGTgca                                                                                 >  8:787747/1‑148 (MQ=11)

                                                                     cgccgcggccgcgcttttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGACAGTCATTCATGTATCTGCCCGTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAAc                                                                               <  5:633495/136‑1 (MQ=11)

                                                                      cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGAGTCGTGGAAAGTCAATAAATATTAAGCCCCAACAAAAACAAAAACCCGCAG‑AAG‑AA‑GGTTTTTAAGAAAATGAAGAGaaaat                                                                              >  4:1398729/1‑149 (MQ=2)

                                                                          actaaGGGGTTTTTTAGGTAAACCAGGTGTAAGTTATTGATATGTCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGAcc                                                                          <  2:586358/148‑1 (MQ=11)

                                                                           cgtatcgtttttttAGGTAAAGGAGGTCAAACTGATCGATAATCCGCTTTCTTTTGGGTATATCGTCGTGGAGATTCATTGATCTTTCTGCCCCTCAAAAATCATAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCg                                                                         <  7:927369/142‑1 (MQ=11)

                                                                           cgAAGCGGTTTATTACGTACAACATGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGCGTCGGCGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GTTTTTTACGTAAATCAGGTGAAACTGACCg                                                                         <  1:965909/151‑1 (MQ=11)

                                                                           cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGGGCCCCTCCAAAACCAAAAACCCGCCG‑CAG‑CG‑GGGTTTTTCGTAAATCAGGTGCAAATGAccc                                                                         >  1:419660/1‑150 (MQ=11)

                                                                           cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCAATCATATTTGTGCCCCGGCAAAAGCAAAAACCCGCCG‑AAG‑GG‑GGTTTTTACGGAAATCACGTGAAACTGTccc                                                                         >  3:1728748/1‑150 (MQ=11)

                                                                           cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTCATGATTCTACGCCAGCAAAAACAAAAACCCGCCGCAGG‑CC‑GGTGTTTACG‑AAATGAGGTGAAACTTAccc                                                                         >  7:156462/1‑150 (MQ=11)

                                                                            gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGTTTGGGCGCCTCCAAAACCAAAAACCCGCCG‑AAG‑CG‑GGTGGTTACGTTAATCAGGTGAAAATGCCCgt                                                                        >  1:229440/1‑150 (MQ=11)

                                                                            gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGCAATCATCATTGAGCCCCCCCAAAACCAAAAACCCGCCC‑AAG‑CC‑CGGGTTTAACTAAATCAAGTGAAAATGACCg                                                                         >  8:835206/1‑150 (MQ=11)

                                                                             aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTAGAGGCGAGGCAATCAAGATAGAACACCGTCAAAAACAAAAACCCGCAT‑CAG‑AG‑CGTTATTAAGGAAATCAAGTGAAATTTACCCAt                                                                       >  4:964028/1‑151 (MQ=2)

                                                                              accggtgtattACGTACACCAGCTTATTATTACCTATAAGCAGCTTTCTTATGCGAAACGGTTATTTGACTGTAATTCATCTTTCTGCGCCTCCCAAAGCTAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTTAAACTGACCGATa                                                                      <  5:1493219/142‑1 (MQ=2)

                                                                              aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGCAATCATATTTGGGCGCCGGCAAAAACAAAAACCCGCCT‑AAGCCG‑GGTGTTTA‑GGAAATCAGGGGAAACttccccat                                                                       >  5:1730039/1‑143 (MQ=2)

                                                                              aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGAAATCAAAATTCTGCACCAACAAAAACAAAAAACCCCCG‑AAG‑CC‑GGGTTTTAAGTAAATCAGCTGAAAcatacccat                                                                       >  6:96648/1‑142 (MQ=2)

                                                                                cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTAATTCATCGTTATGCCCCGGCAAAACGAAAAACCCGCCG‑CAG‑CG‑GGTTTTTAAGGAAATCAGGTGTAACTGCCCGATa                                                                      >  8:1532645/1‑149 (MQ=11)

                                                                                 gggTTTTTACGTAAACCAGGCGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTAAATAATAATTAAGCACCTACAAAAACAAAAAACCCCCT‑AAC‑AG‑GGATATTAAATAAATCAAGTGAAAATGACAGATaaac                                                                   >  2:232012/1‑149 (MQ=2)

                                                                                   gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATAGTTAGGCCCCGGCAAAAGCAAAAACCCGCCG‑CAG‑CG‑GGGTTTTACGTAAATCAGGTGCAACTTCCCGATAAGCCg                                                                 >  3:738182/1‑151 (MQ=11)

                                                                                     ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTCATAATTGTGCCCCAACAAAAACAAAAAACCGCCG‑CAG‑AC‑GGGGTTTAAGGAAATCAAGTGAAAATTACCCATAAGCCggg                                                               >  1:1753995/1‑151 (MQ=2)

                                                                                     ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCGGCCCCGCCAAAAGCAAAAACCCGCCG‑AAG‑CC‑GGTTTTTTAGTTAATGACGTGCAACTTACCCTTAAGCCggg                                                               >  8:760258/1‑151 (MQ=11)

                                                                                     ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGCCAGTCATTCATGGTTCGGCGCCGACAAAAACAAAAACCCGCAG‑CAG‑CC‑GGGTTATAAGGAAATCAGGTGAAAATTACCCTTAATCCggg                                                               >  6:1779032/1‑151 (MQ=2)

                                                                                      tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTGATGTTTACGCCCCGCCAAAAGCAAAAACCCGCCG‑CAG‑CG‑CGGTTTTTCGTAAATCACCTGAAACTTCGCCTTAAGCCGGGt                                                              >  1:1138571/1‑151 (MQ=11)

                                                                                       ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCCATCATCTTTCGGCGCCAACAAAAACAAAAACCCGCAG‑CAG‑CC‑CGTTTTTAAGTAAATCACGTGAAACTTACCCATAATCCggggt                                                             >  8:1445438/1‑149 (MQ=11)

                                                                                       ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGAGCGCCTGCAAAAGCAAAAACCCGCCG‑AAGACG‑GGTTTTT‑CGTAAATCACGTGCAAATTTCCCATAAGCCGGGtt                                                             >  3:1183029/1‑151 (MQ=14)

                                                                                        tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCATTCATCTTTGAGCCCCTCCAAAAGCAAAAACCCGCCG‑CAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTTCCCGATAAGCCGGGttg                                                            >  5:1635060/1‑150 (MQ=14)

                                                                                        tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATATTTAAACGCCAACAAAAACAAAAACCCGCGG‑CAG‑CG‑CGTTTTTACGTAAATCACGTGAAAAATACCGATAATCCGGGttg                                                            >  6:1685639/1‑150 (MQ=14)

                                                                                         aCGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCTTTCAGCACCTTCAAAACCAAAAACCCGGCG‑AAG‑CG‑CGGTTTTAACTAAATCACGTGAAAATTACCGATAATCCGGGTTCt                                                           >  1:650059/1‑151 (MQ=14)

                                                                                          cGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                                   <  3:1042184/97‑1 (MQ=12)

                                                                                          cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCATTCATATTTGGTCGCCGGCAAAAACAAAAACCCGCAG‑CAG‑GG‑GGTTTTTACGGTTATGAGGTGCTAGTGGGCGATAATCCGGGTtgtg                                                          >  2:1159388/1‑151 (MQ=2)

                                                                                            tAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGAGTCAAGTCAATCAATATGTAACACCGTCAAAACCAAAAACCCGCCG‑ACA‑AC‑GGATTATACGGAAATAAGGTGAAACTGACGGATAAGCGGGGTTcgggg                                                        >  2:36579/1‑147 (MQ=9)

                                                                                             aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCATTCATCTTTGGGCCCCGCCAAAAGCAAAAACCCGCCG‑CAG‑CC‑GGGTTTTTCGTGAATCAGGGGCAACTGAGCGCTAAGCCGGGTTgtgtgg                                                       >  6:646453/1‑149 (MQ=14)

                                                                                               aacatgcgAAACTCACCGATCAGCCGCTTTCTTTTCCTTATAGGGTCGTGGACAGGCATTCTTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTg                                                     <  5:543459/144‑1 (MQ=14)

                                                                                                ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCATTCATGTTTAGGCCCCACCAAAAGCAAAAACCCCCCG‑AAG‑CG‑GGGGTTTAAGTTAATCCCGGGCAACAGGCCGCTCAGCCGGGTTCTGtgcggg                                                    >  3:1452990/1‑146 (MQ=9)

                                                                                                 cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGTCACTCAATCAACATTGGGCCCCCCCAAAAACAAAAACCCCCCG‑CAC‑CC‑CCGGTTTAAGGAAATAAAGTGGAAATGCCCGAGAAGCCGGGtggtgggggggg                                                   >  6:839587/1‑140 (MQ=2)

                                                                                                 cAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTAGTGGAGAGTCAATCATATTTATACACCACCAAAAACAAAAAACCCCCA‑AAC‑AA‑CGTTTTTAAGATAATCAAGAGAAAATTCCCCATAATCCGGGtaaagtagagc                                                    >  2:279186/1‑140 (MQ=2)

                                                                                                  cggTAAAACTGACCGATAAGCCGCTTTTTTTTTTTTTTATCGTCGTGGACAGTCATTTATATTTCTGACGATACTATATCAAAAAACGACGG‑TAT‑TG‑GGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGAc                                                  <  7:324447/150‑1 (MQ=9)

                                                                                                  aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGTAAAGTAATTCAAATTTCAGCCCCTTCAAAATCAAAAACCCGCAG‑CAT‑CC‑GGGTTTTACGTAAATCACGTGAAAATTACCAAAAACCAGGGTTCAGTCGTgggt                                                  >  6:109292/1‑149 (MQ=9)

                                                                                                        aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCACCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGGTTTTACGTAAATGAGGTGAAAATGACCGATAAGCCGGGTTCTGTGGTGGACAGtcat                                            >  7:438832/1‑151 (MQ=17)

                                                                                                         aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTTTCTTTTAAATTCATTCATCTTTCTGCCACTCCAAAAGAAAAAACCAGACG‑AAT‑AG‑GGTTTTTACGTAAATCAGGTTATACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                                 <  6:1508893/145‑1 (MQ=14)

                                                                                                           tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCATTCATCTTTCAGCCCCTACAAAAGCAAAAACCCGCCG‑AAG‑CC‑GGGGTTTACGTAAATGACGTGAAACTGACCGATAAGAAGGGTTCTGTCGTGGCAAGtgattc                                          >  4:144430/1‑150 (MQ=14)

                                                                                                            gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCG‑CAG‑CG‑GGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTGGTGGACAGtcattcat                                        >  1:353375/1‑151 (MQ=255)

                                                                                                            gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCGCCAAAAACAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCACGTGAAACTGACCGATAAGCAGGGTTATGTAGTGGAAAGtcattaat                                        >  4:740117/1‑151 (MQ=14)

                                                                                                             aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACACGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCCATAAGCCGGGTTGAGTCGTGGACAGtcattcct                                        >  3:1564612/1‑148 (MQ=255)

                                                                                                              ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTATGCCCCACAAAAAACAAAAAACCCCCC‑AAA‑CC‑GCTTTTTACGTAAACAAGGTGAAAAAGACCCAAAACCCGCGTTATGTAGGGGaaacgcaagggga                                       >  6:599076/1‑138 (MQ=2)

                                                                                                               cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAACCAAAAACCCGCCG‑AAG‑CG‑GGGTTTTACGTAAATCAGGTGAAACTGCCCGCTAAGCCGGGTTCTGTCGGGGCCCCGCATTCATCt                                      >  8:438054/1‑150 (MQ=14)

                                                                                                                 aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGGCGGGTTATGTGGTGGACAGTCATTCAGCTAgg                                   >  2:1734379/1‑151 (MQ=255)

                                                                                                                  tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTCGTGGACAGTCATTCATCTAGGc                                  >  8:1149769/1‑151 (MQ=255)

                                                                                                                    aGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑Cg‑g                                                                                                       <  3:1275711/80‑1 (MQ=33)

                                                                                                                      ccGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCCTTAATGTTTCTGCCCCTCCAAAAGAAAAAACCCCCCG‑CAC‑CG‑GGTTTTTACGTAAAACAGGTGAAACAGCCCCATAAGCCGGGTTCTTTTGGGTAGAGTCTTTGATCAAAGCCAGc                              >  5:491949/1‑151 (MQ=2)

                                                                                                                      ccGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTAATTCATATTTCAGCCCCTACAAAAGAAAAAAACCCCCC‑AAG‑CG‑CGGTTTTACCTAAATAATGTGAAACTTCCCGAAAAACCGGGTTCAGTCGAGGACAGTCATACATCTATGCCacc                              >  7:670305/1‑149 (MQ=12)

                                                                                                                       cGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTGATCTTTTTGCCCCTCCAAAAGCAAAAAACCCCCG‑AAG‑CG‑GGTTTTTAAGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTGATCTAGGCCAgg                              >  8:381427/1‑149 (MQ=18)

                                                                                                                        gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTGTGCCCCTCCAAAAGCAAAAACCCCCCG‑AAG‑CG‑CGTTTTTACCAAAATAAGCTGAAACTGACCGATAAACCGGGTTCACTCGTGGACAGTCATTCATCTAGGccacca                             >  6:1408503/1‑150 (MQ=17)

                                                                                                                          tttctttTGGGTATAGCGTCGTGGCCAGTAATTGATCTTTGTGCCCCGCCAAAACCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTGTGTGGTGGCACGTCATTCATGTAGGCCAGCAATc                          >  3:394123/1‑151 (MQ=17)

                                                                                                                          tttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTCGTGGACAGTCATTCATCTAGGCCATCAAt                           >  2:134947/1‑150 (MQ=255)

                                                                                                                           ttctttTGGGTATAGCGTCGTGGACAGTCATTCATATTTATGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGACAGTCATTCATCTAGGCCAGCAATCg                         >  4:1240541/1‑151 (MQ=255)

                                                                                                                           ttctatTTGGGATAGCGTCCTCGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                          <  1:450808/150‑1 (MQ=255)

                                                                                                                           gtctttTGCGTATAGTGTCGGGGACAGTCATTCATCTTACTGCCACTCCAAAAGCAAAAACCCTCCG‑AAG‑AG‑TGTTTTTTGGTAAATCAGGCTAAACTGAGCTATATTGCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg                         <  8:285996/150‑1 (MQ=255)

                                                                                                                            gctttTGGGTATAGGGTCGTGGACAGTAATTCATATTTCAGCCCCTACAAAAGCAACAACCCGGAG‑ATG‑CG‑GGTTTTTACGTAATGCAGGTTAATCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc                        <  3:808410/150‑1 (MQ=18)

                                                                                                                             ctttTGGGTATAGCGTCGTGGGCAGTCATTGATCTTTGTGCCCCTCCAAAAGCAAAAAAACGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCATTCATCTAGGCCCGCAATCggt                       >  3:1672801/1‑149 (MQ=18)

                                                                                                                             ctttTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTGTGCCCCGCCAAAAGCAAAAAACCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATATAGGCCCCCAATAGcg                       >  1:1678863/1‑150 (MQ=17)

                                                                                                                             ctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCGGGGTTCTGTCGTGGCCAGTCCTTCATCTAGGCCAGCAATCGCt                       >  3:1544091/1‑151 (MQ=255)

                                                                                                                                ttGGGTATAGCGTCGTGGACAGTCAATAAACTTTATGCCCCACCAAAAACAAAAACCCCCCG‑AAA‑AG‑GGTTTTTAAGTAAATCAGGTGAAAATGACCGAAAAGCCGGGTTCTGTCGTGGACAGTCATTAATATAGGCCAGCAAtacctcgc                    >  6:426851/1‑144 (MQ=255)

                                                                                                                                    gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CC‑GGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAGCTAGGCCAGCAATCGCTCACTGGc                >  2:63981/1‑151 (MQ=255)

                                                                                                                                    gTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAAACCGCCG‑AAC‑CG‑GGTTTTTAAGTAAATCAGGTGAAACTGACCGAAAAGCCGGGTTCTGGCGTGGACAGTCATTCATCTAGGCCACCAATCGCTCACTGGc                >  7:1222871/1‑151 (MQ=21)

                                                                                                                                     tataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGcg               >  4:1153930/1‑150 (MQ=255)

                                                                                                                                           gtcgtcGACAGTCTATCTTCTGTCTGCCCCTCCATACGGAAAATCCGGCCT‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca         <  8:1206611/151‑1 (MQ=25)

                                                                                                                                              gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc      >  2:881112/1‑151 (MQ=255)

                                                                                                                                              gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc      >  1:492444/1‑151 (MQ=255)

                                                                                                                                               tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGTGGAGAGTCATTACGCTAGGCCATCAATCGGTACCTCGCTCAaccacac      >  3:1126094/1‑147 (MQ=14)

                                                                                                                                                 gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc   <  1:1178979/151‑1 (MQ=255)

                                                                                                                                                  aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc  >  6:584910/1‑151 (MQ=255)

ATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270005‑3270301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`