Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1670CG23.1% 398.4 / 40.7 279intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base C (98/75);  new base G (51/1);  total (193/86)
Fisher's exact test for biased strand distribution p-value = 7.41e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

GTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270031‑3270317
                                                                                                                                        |                                                                                                                                                        
       aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGGATAGCGTCGAGGCCAGTCAAGCATAGTATAGCACCTCCaaaaacaaaaac                                                                                                                                     >  8:553605/1‑151 (MQ=11)
           tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGAGGCTAGGCATTCATGGTTGCGCGCCTCCAAAAGCAAAAACccgc                                                                                                                                 >  1:1048108/1‑151 (MQ=11)
           caacacctcgctCTTCCCATCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACATTTTAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                                                                                                 <  6:505062/140‑1 (MQ=11)
            gtttgatCTTTCTATCACTCCAAAATCTTAAACCCGCCGTATCGGGTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                                <  8:1655197/146‑1 (MQ=11)
              ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGCAACTAAATCAAAATTGTGCCCCACCAAAAGCAAAAACccgccgc                                                                                                                              >  1:878494/1‑150 (MQ=11)
                 atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGGGTAGAGCGACGGGGCAAGGCCTGCATGAGGGAGCGCCAACAAAAACAAAAACCCGCCGAAgg                                                                                                                           >  7:183832/1‑150 (MQ=2)
                  tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGCGAGTCAATCATGTTTTGGCGCCGGCAAAACCAAAAACccgggtaagcg                                                                                                                          >  1:206012/1‑143 (MQ=11)
                   cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGTGTATAGCGTCTTGTAAAGTCCATCAAAATTAAGCACCGTCAAAACCAAAAACccggagcagt                                                                                                                           >  2:1688398/1‑142 (MQ=11)
                    tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGAGAGCGTCGAGGCGAGTCATTCATCTTTATGCGCCACCAAAACCAAAAACCCGCAGaaccccg                                                                                                                        >  8:1699890/1‑146 (MQ=11)
                    tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTGGGGTAAAGCGTCGTGGCCAGTCCTTCAAAGTTAGCCCCCCACAAAAGCAAAAACCCCCCGCAG‑Cgcg                                                                                                                       >  3:538067/1‑149 (MQ=11)
                     ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAGCAGGTGAACCTGACCGATAAGCCGCGTTCTGTTGGGTAGAGCGTCGTGGCGAGGCCTTCATCCTGGGGCCCGCGCAAAACCAAAACCccgcctcagggggg                                                                                                                       >  1:1562809/1‑142 (MQ=2)
                        tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTAGGGGATACCGTCGAGGACAGGCAATCATCTTTACGCAGCGGCAAAACCCAAAACCCGCCTCAG‑CG‑CGtgtt                                                                                                                  >  6:1706705/1‑151 (MQ=11)
                           cccTCCACAATCAAAAACCCGCCGGACCGGGTTTTTACGTAAACCATGTGAAACTGACCGATAAGCCGCATTCTTTTGGGTATAGCGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTAc                                                                                                               <  5:1272125/151‑1 (MQ=11)
                           cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTCGTGGACAGGCAATCAAAGTTCGACGCCACCAAAACCAAAAACCCCCCGCAgagcgggattt                                                                                                                  >  6:365033/1‑140 (MQ=11)
                               ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAGGCCGCTTTCTTTTGGGTATAGCGTCGTGGGGCGTGATTCATGATTGTGGCCCGCCAAAAGCAAAAACCCGCCGCCG‑CG‑GGGTTGTACGtt                                                                                                            >  5:601584/1‑149 (MQ=11)
                                 acaatctgaaaCCCGCCGCACCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAAt                                                                                                         <  4:1262596/143‑1 (MQ=11)
                                 aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTCATCGTTGTGCCCCTGCAAAACCAAAAACCCCCCGAAG‑CC‑GGGTGTTAccgtaat                                                                                                         >  3:1289500/1‑145 (MQ=11)
                                   aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCATCGTTATGCGCCGCCAAAAGCAAAAACCCGCCGCAG‑GC‑GGGTGTTACggtaatg                                                                                                        >  2:1187703/1‑144 (MQ=11)
                                    agcccaaaCCCGCCGCACCGGGTTATTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAg                                                                                                      <  8:432057/146‑1 (MQ=11)
                                      cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCCTTCCTGGTGCTGCCCGGGCAAAAGCAAAAACCCGCCGCAG‑CGCGGTGTTTaggaaatgacgg                                                                                                     >  3:1505931/1‑140 (MQ=11)
                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGGCCTTGATGGTTGTGGCCCGGCCAAAACAAAAACCCGCCGCAG‑CG‑GGgtttttagtttatgagggg                                                                                                   >  5:697039/1‑137 (MQ=11)
                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATAAAATTTAAGCACCAACAAAAACAAAAAACCGCCGAAG‑CG‑CGTTTTTAACTAAATCAAGTg                                                                                                   >  4:1462794/1‑151 (MQ=11)
                                         aaaCCCGCCGAAGCTGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTTGTATAGCGTCGTGGACAGTCATTCATCTTTCTGTCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGaa                                                                                                 <  6:1365298/151‑1 (MQ=255)
                                          aaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAGGTTGTGCCCCAGCAAAACCAAAAACCCGCCGCAG‑CG‑GGGGTTTAAGTTAATCAGGTgca                                                                                                 >  8:787747/1‑148 (MQ=11)
                                              cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCATCATGGTTCTGCCGCAACAAAACCAAAAACCCGCCGAAG‑CG‑CGGTAGTACGGTAATCAGGTGaaaattt                                                                                            >  4:1013007/1‑147 (MQ=11)
                                                actaaGGGGTTTTTTAGGTAAACCAGGTGTAAGTTATTGATATGTCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGAcc                                                                                          <  2:586358/148‑1 (MQ=11)
                                                 cgAAGCGGGTTTTTACGTAAACCAGGTGAATTTTTTTTATTTTTCGGTTTATTTTGGTTATATAGTCGTGGTAAGTCATTCTTTTTTTTGACGATGCTATTGCTATCTCCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCg                                                                                         <  2:389279/151‑1 (MQ=2)
                                                 cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGGGCCCCTCCAAAACCAAAAACCCGCCGCAG‑CG‑GGGTTTTTCGTAAATCAGGTGCAAATGAccc                                                                                         >  1:419660/1‑150 (MQ=11)
                                                 cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCAAGTTTGTGCCCCTCCAAAAGCAAAAAACCGCCGAAG‑CC‑GGGTTTTACGTAAATCAggggcaaattcccc                                                                                         >  1:839982/1‑139 (MQ=11)
                                                  gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGTTTGGGCGCCTCCAAAACCAAAAACCCGCCGAAG‑CG‑GGTGGTTACGTTAATCAGGTGAAAATGCCCgt                                                                                        >  1:229440/1‑150 (MQ=11)
                                                  gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGCAATCATCATTGAGCCCCCCCAAAACCAAAAACCCGCCCAAG‑CC‑CGGGTTTAACTAAATCAAGTGAAAATGACCg                                                                                         >  8:835206/1‑150 (MQ=11)
                                                   aagagagTTTTTACCTACACGACGTTATTCTGATCTATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGAt                                                                                       <  2:1132935/145‑1 (MQ=11)
                                                    accggtgtattACGTACACCAGCTTATTATTACCTATAAGCAGCTTTCTTATGCGAAACGGTTATTTGACTGTAATTCATCTTTCTGCGCCTCCCAAAGCTAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTTAAACTGACCGATa                                                                                      <  5:1493219/142‑1 (MQ=2)
                                                    aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTCAACGTTGGGCCCCGGCAAAAGCAAAAACCCGCCGCAG‑CG‑GGTTTTTAAGTAAATCATGTGAAACTGCCCgttc                                                                                      >  3:182898/1‑148 (MQ=11)
                                                      cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTAATTCATCGTTATGCCCCGGCAAAACGAAAAACCCGCCGCAG‑CG‑GGTTTTTAAGGAAATCAGGTGTAACTGCCCGATa                                                                                      >  8:1532645/1‑149 (MQ=11)
                                                          tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTGGTGGAAAGTCAATCATGATGATGCGCCAGCAAAAGCAAAAACCCGCGGAAG‑AA‑CGGTTGTAAGGAAATCAGCAGAAAATTGCCCATAATCCgg                                                                                >  7:255213/1‑151 (MQ=2)
                                                           ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCGGCCCCGCCAAAAGCAAAAACCCGCCGAAG‑CC‑GGTTTTTTAGTTAATGACGTGCAACTTACCCTTAAGCCggg                                                                               >  8:760258/1‑151 (MQ=11)
                                                            tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTGATGTTTACGCCCCGCCAAAAGCAAAAACCCGCCGCAG‑CG‑CGGTTTTTCGTAAATCACCTGAAACTTCGCCTTAAGCCGGGt                                                                              >  1:1138571/1‑151 (MQ=11)
                                                             ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGAGCGCCTGCAAAAGCAAAAACCCGCCGAAGACG‑GGTTTTT‑CGTAAATCACGTGCAAATTTCCCATAAGCCGGGtt                                                                             >  3:1183029/1‑151 (MQ=14)
                                                               aCGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCTTTCAGCACCTTCAAAACCAAAAACCCGGCGAAG‑CG‑CGGTTTTAACTAAATCACGTGAAAATTACCGATAATCCGGGTTCt                                                                           >  1:650059/1‑151 (MQ=14)
                                                                  tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTACAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTTCCCGATAATCCGGGTTCTGtg                                                                        >  7:133786/1‑150 (MQ=255)
                                                                   aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCATTCATCTTTGGGCCCCGCCAAAAGCAAAAACCCGCCGCAG‑CC‑GGGTTTTTCGTGAATCAGGGGCAACTGAGCGCTAAGCCGGGTTgtgtgg                                                                       >  6:646453/1‑149 (MQ=14)
                                                                    aaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTCATGTTTGTGCCCGGACAAAAGCAAAAACCCGCCGCAG‑GG‑GGGGTTTTAGGAAATCAGGGGAAAGTGAGCGATAAGCCGGGTTCTGtggg                                                                      >  4:95806/1‑148 (MQ=14)
                                                                     aCCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGAGAGTCCTTCATGTTTGATCACCAACAAAAGCAAAAACCCGCCGAAG‑AG‑CGTTTTTACGTAAATCAGGTGAAAATGACAGCTACACCGGGTTTTgtggtg                                                                     >  8:1095363/1‑151 (MQ=14)
                                                                     aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGACAGTCATTCATAATTATGAACCACCAAAAACAAAAACACGCAGACG‑CC‑GGTTTTTAAGTAACTGAGGGGAAACTTACAGATAATCCGGGTTCTGtag                                                                       >  2:901714/1‑147 (MQ=9)
                                                                      ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCATTCATGTTTAGGCCCCACCAAAAGCAAAAACCCCCCGAAG‑CG‑GGGGTTTAAGTTAATCCCGGGCAACAGGCCGCTCAGCCGGGTTCTGtgcggg                                                                    >  3:1452990/1‑146 (MQ=9)
                                                                       cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGTCACTCAATCAACATTGGGCCCCCCCAAAAACAAAAACCCCCCGCAC‑CC‑CCGGTTTAAGGAAATAAAGTGGAAATGCCCGAGAAGCCGGGtggtgggggggg                                                                   >  6:839587/1‑140 (MQ=2)
                                                                       cAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTAGTGGAGAGTCAATCATATTTATACACCACCAAAAACAAAAAACCCCCAAAC‑AA‑CGTTTTTAAGATAATCAAGAGAAAATTCCCCATAATCCGGGtaaagtagagc                                                                    >  2:279186/1‑140 (MQ=2)
                                                                        aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGTAAAGTAATTCAAATTTCAGCCCCTTCAAAATCAAAAACCCGCAGCAT‑CC‑GGGTTTTACGTAAATCACGTGAAAATTACCAAAAACCAGGGTTCAGTCGTgggt                                                                  >  6:109292/1‑149 (MQ=9)
                                                                        aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATATTTAGGCCCCTACAAAAGCAAAAACCCGCCGAAG‑AG‑GGGTTTTAAGTAAATCAGGTGCAACTGACCGATAAGCCGGGTTgtgtagggggc                                                                  >  6:538874/1‑144 (MQ=11)
                                                                              agcagACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGTAGTTTAATTCATCTTTCTGCCCCTACAAATTCAAAAACCCGCTGAAG‑GT‑GGTTTTTACGTAATTCAGGTGTATCTGACCGATAAGCGGGGTTCTGTCGTGGACAGtcat                                                            <  4:1424527/147‑1 (MQ=14)
                                                                              aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCACCAAAAGCAAAAACCCGCCGAAG‑CG‑GGGTTTTACGTAAATGAGGTGAAAATGACCGATAAGCCGGGTTCTGTGGTGGACAGtcat                                                            >  7:438832/1‑151 (MQ=17)
                                                                               aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTTTCTTTTAAATTCATTCATCTTTCTGCCACTCCAAAAGAAAAAACCAGACGAAT‑AG‑GGTTTTTACGTAAATCAGGTTATACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                                                 <  6:1508893/145‑1 (MQ=14)
                                                                                atgacctttattgttctttgtttTGGGTATAGAGTGTTTTATAGTCATTCTTCTTTATGCACTTCCTAAAGGAATATCTTGCGTAAG‑CG‑GTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                                          <  7:1644951/136‑1 (MQ=255)
                                                                                  gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGCAG‑CG‑GGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTGGTGGACAGtcattcat                                                        >  1:353375/1‑151 (MQ=255)
                                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCATCATTAAGCACCAACAAAACCAAAAACCCGCCGAAG‑AC‑GCTTTTTAAGTAAATCACGTGAAAATTACCCATAATCAGGGTTGTGTTGTGGAAAgggttgaa                                                         >  7:1029703/1‑142 (MQ=2)
                                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACACGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCCATAAGCCGGGTTGAGTCGTGGACAGtcattcct                                                        >  3:1564612/1‑148 (MQ=255)
                                                                                     cGATAAGCCGATTTCTTTTGGTTATATTTTTTTTGATAGTCATTCATCTTTCTGCAACTCATAAATAAAAAATCTTTCGAAG‑CT‑GGTTTTTATTTAAATCTGGTTAATCTGATCGATAATCCGTTTTCTGTCGTGGACAGTCATTCATCTa                                                     <  2:217267/151‑1 (MQ=255)
                                                                                      gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTTAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCCTTCATCt                                                      >  7:854003/1‑149 (MQ=255)
                                                                                      gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAATCAAAAACCCCCCCAAA‑CG‑GGTTTTTAACTAAATCAGGTGAAACTGACCGATAAGCCCCGTTATGTCGTTGACAGTCATTCATCt                                                      >  2:741519/1‑149 (MQ=14)
                                                                                       aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCATTAATCTAgg                                                   >  6:590347/1‑151 (MQ=255)
                                                                                        gacgCCGCTTCCTATCGGGCATAGAGTCGTGGACAGTCATTCATCTTTCTTCCAATCCAAACCCAAAATCCCGCCGAAG‑CT‑TTTTTTTACGTAATTCAGCTGAAAGTTACCGATATGCCGGTTTCTGTCGTGGACAGTCATTCATCTAGGc                                                  <  5:541681/148‑1 (MQ=12)
                                                                                            ccGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTAATTCATATTTCAGCCCCTACAAAAGAAAAAAACCCCCCAAG‑CG‑CGGTTTTACCTAAATAATGTGAAACTTCCCGAAAAACCGGGTTCAGTCGAGGACAGTCATACATCTATGCCacc                                              >  7:670305/1‑149 (MQ=12)
                                                                                             cGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGTGCCCCGCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTATGCCAGc                                              >  5:1038756/1‑150 (MQ=255)
                                                                                              gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTGATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑CG‑GGTTTTTACGTAAATAAGGTGGAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCATTCATCTAGGCCAGCa                                             >  3:784439/1‑150 (MQ=255)
                                                                                              gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTCGTGGACAGTCATTCATCTAGGccacca                                             >  5:1678855/1‑150 (MQ=255)
                                                                                               ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                                           >  3:353336/1‑151 (MQ=255)
                                                                                                 gtctttTGCGTATAGTGTCGGGGACAGTCATTCATCTTACTGCCACTCCAAAAGCAAAAACCCTCCGAAG‑AG‑TGTTTTTTGGTAAATCAGGCTAAACTGAGCTATATTGCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg                                         <  8:285996/150‑1 (MQ=255)
                                                                                                   ctttTGGGTATAGCGTCGTGGGCAGTCATTGATCTTTGTGCCCCTCCAAAAGCAAAAAAACGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCATTCATCTAGGCCCGCAATCggt                                       >  3:1672801/1‑149 (MQ=18)
                                                                                                   ctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCGGGGTTCTGTCGTGGCCAGTCCTTCATCTAGGCCAGCAATCGCt                                       >  3:1544091/1‑151 (MQ=255)
                                                                                                      ttGGGTATAGCGTCGTGGGCAGTCATTGATGTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑CG‑GGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCCGTCATTGATCTAGGCCCGCAATCGCTccc                                    >  1:60282/1‑149 (MQ=18)
                                                                                                       tGGGTATAGGGTCGTGGACATTCATTCATCTTTCTGCACCTCCAAAAGCAAAAACGGGCATAAG‑CG‑TGTTTTTACTTAATTCTGGTTAAACTGATGGATTAGGGGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCAc                                    <  2:1386307/150‑1 (MQ=18)
                                                                                                           tataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGcg                               >  4:1153930/1‑150 (MQ=255)
                                                                                                              aGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAAACCGCCGAAG‑CG‑GGTTTTTACGTAAATAAGGTGAAAATTACCGATAAGCCGGGTTATGTGGTGGCCAGTCATTCATCTAGGCCCGCAATAGCTCACTGGcaca                             >  4:1378243/1‑150 (MQ=18)
                                                                                                                 gtcgtcGACAGTCTATCTTCTGTCTGCCCCTCCATACGGAAAATCCGGCCTAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                         <  8:1206611/151‑1 (MQ=25)
                                                                                                                 gtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          >  1:1225876/1‑150 (MQ=255)
                                                                                                                   cgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCAAGCAATCGCTCACTGGCTCAagcagc                       >  5:1364128/1‑151 (MQ=255)
                                                                                                                     tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGTGGAGAGTCATTACGCTAGGCCATCAATCGGTACCTCGCTCAaccacac                      >  3:1126094/1‑147 (MQ=14)
                                                                                                                     gggACAGGCATTCATCTTGCTGCCCCTCCAACAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     <  5:868338/150‑1 (MQ=255)
                                                                                                                      gtaCAGTGATTCATCTTTATGCCCCTCCAAAATCAAAATTCCGCCGTAG‑CT‑TGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     <  7:968614/148‑1 (MQ=25)
                                                                                                                        aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc                  <  5:42105/151‑1 (MQ=255)
                                                                                                                          aGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGTTTCTGTGGGGGAGAGTGAGGGGTCGAGGCCAGCAAAGGGTCCCCCCCTCAAGCACCCAAcccc                >  8:1428251/1‑150 (MQ=2)
                                                                                                                           ggcattcctCTTTCTGCCCCTCCAAAAGCAAATGCCGGGCTCAG‑GT‑GGTTTTTTCGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg               <  5:1731168/149‑1 (MQ=25)
                                                                                                                           gTCTTTCTTTTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg               <  6:1791417/151‑1 (MQ=255)
                                                                                                                            tacttcatATTTCTGTCTCTCCTTAATCTAATAGCTTCCGATG‑TG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg               <  7:1269435/147‑1 (MQ=37)
                                                                                                                             cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt             >  6:1410151/1‑151 (MQ=255)
                                                                                                                             cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGATCACTGGCTCAAGCAGCCTACCAGGGt             >  8:893992/1‑151 (MQ=255)
                                                                                                                              attcatCTTTCTGCCCCTGCAATATAAAAATGCCTCCTAAG‑TT‑TTTTTTTACTTAATTCTGGTGAAAGTGATTGATTTTGGGTTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGtt            <  2:327021/151‑1 (MQ=255)
                                                                                                                              attcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattcat                                                        <  3:882476/107‑1 (MQ=255)
                                                                                                                              attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgggac            >  3:1757929/1‑149 (MQ=255)
                                                                                                                              attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg              <  4:1755456/149‑1 (MQ=255)
                                                                                                                               ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           >  1:46413/1‑151 (MQ=255)
                                                                                                                                tcatGTTTATGCCCAACAAAAAGCAATACCCCGAGTAAT‑GT‑GGTTTTTACTTAAATCAGGTGAAACTGCCCGATATTCCGGCTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           <  3:1041392/150‑1 (MQ=255)
                                                                                                                                   tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGt        >  7:1168810/1‑150 (MQ=255)
                                                                                                                                    atttGTGCGCCTCCAACATCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTAc      <  3:1732464/150‑1 (MQ=255)
                                                                                                                                     tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     >  4:326198/1‑151 (MQ=255)
                                                                                                                                     tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     >  8:695461/1‑151 (MQ=255)
                                                                                                                                     tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     >  1:1739825/1‑151 (MQ=255)
                                                                                                                                      ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACgg    >  1:940089/1‑151 (MQ=255)
                                                                                                                                        cTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGc  >  6:1214113/1‑151 (MQ=255)
                                                                                                                                        |                                                                                                                                                        
GTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CG‑GGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270031‑3270317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑