Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,907,327 G→A Q37* (CAG→TAG)  cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,907,3270GA94.8% 1384.2 / 40.9 400Q37* (CAG→TAG) cspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base G (8/13);  new base A (185/194);  total (193/207)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 400 total aligned reads displayed.

TATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGC  >  NC_000913/1907180‑1907473
                                                                                                                                                   |                                                                                                                                                  
 atatctagaTTGAAGCGTATTCACATTTCAGATCAGTGTATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGTACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAACCTAGAt                                                                                                                                                 <  7:1177237/144‑1 (MQ=255)
 ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGAt                                                                                                                                                 <  7:581999/150‑1 (MQ=255)
 ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATa                                                                                                                                                <  6:821789/151‑1 (MQ=255)
 ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATa                                                                                                                                                <  7:1637343/151‑1 (MQ=255)
   aGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAg                                                                                                                                               <  7:1389627/150‑1 (MQ=255)
   aGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGc                                                                                                                                              <  2:1534822/151‑1 (MQ=255)
      gagaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATGAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGga                                                                                                                                           <  4:1253426/151‑1 (MQ=255)
      gagaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGga                                                                                                                                           <  5:1414268/151‑1 (MQ=255)
        gaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCgg                                                                                                                                            >  5:679654/1‑148 (MQ=255)
          ttGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGgaga                                                                                                                                         >  6:394459/1‑149 (MQ=255)
          ttGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGgaga                                                                                                                                         >  8:96115/1‑149 (MQ=255)
           tGAAGCGTATTCACACTTCAGATCAGTGGAATCGATCAGATACCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCACCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga                                                                                                                                         <  4:1151611/148‑1 (MQ=255)
            gAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgt                                                                                                                                      <  6:1301027/150‑1 (MQ=255)
            gAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgt                                                                                                                                      <  1:1332400/150‑1 (MQ=255)
            gAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACACCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAATCCATTACCCTAGATAGCGGAGAAgtg                                                                                                                                     >  5:404326/1‑151 (MQ=255)
            gAAGCGTATTCACACTTCAGATCAGTGGATGCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgtg                                                                                                                                     >  5:1155347/1‑151 (MQ=255)
             aaGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgtgt                                                                                                                                    <  2:363590/151‑1 (MQ=255)
             aaGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgtgt                                                                                                                                    >  8:74018/1‑151 (MQ=255)
                cGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACg                                                                                                                                 <  2:963880/151‑1 (MQ=255)
                     tCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAacac                                                                                                                            >  8:1415260/1‑151 (MQ=255)
                        cacTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACAtg                                                                                                                         >  2:1031526/1‑150 (MQ=255)
                             cAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgc                                                                                                                    >  5:474075/1‑151 (MQ=255)
                             cAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgc                                                                                                                    >  6:1214274/1‑151 (MQ=255)
                              aGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct                                                                                                                   >  5:1505092/1‑151 (MQ=255)
                              aGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgct                                                                                                                   <  7:1721544/151‑1 (MQ=255)
                               gATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgctg                                                                                                                  <  2:812694/151‑1 (MQ=255)
                                aTCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAATACCCTAGATAGCGGAGAAGTGTACGAACACATCTTagctgc                                                                                                                 >  7:1578337/1‑151 (MQ=255)
                                 tCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGcc                                                                                                                >  6:1012443/1‑151 (MQ=255)
                                   aGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCAt                                                                                                              >  5:824552/1‑151 (MQ=255)
                                    gTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATc                                                                                                             >  8:231632/1‑151 (MQ=255)
                                            gATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg                                                                                                     <  3:1544432/151‑1 (MQ=255)
                                             aTCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt                                                                                                    <  7:1069974/151‑1 (MQ=255)
                                             aTCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt                                                                                                    >  6:921200/1‑151 (MQ=255)
                                              tCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt                                                                                                    >  8:354742/1‑150 (MQ=255)
                                               cAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTa                                                                                                   <  6:1130289/150‑1 (MQ=255)
                                                aGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                                                                                                 <  5:306288/151‑1 (MQ=255)
                                                       tGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc                                                                                           >  3:176336/1‑150 (MQ=255)
                                                       tGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCAATCAGCCGGAGTAATGAAGcc                                                                                           >  2:695683/1‑150 (MQ=255)
                                                        gTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc                                                                                           <  4:302426/149‑1 (MQ=255)
                                                            cGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAcc                                                                                     <  3:1472412/151‑1 (MQ=255)
                                                             gTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCt                                                                                    <  7:944163/151‑1 (MQ=255)
                                                                aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa                                                                                 <  6:1329132/151‑1 (MQ=255)
                                                                 aCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg                                                                                <  8:1226624/151‑1 (MQ=255)
                                                                 aCAGCTGCCGGACCTTTCTGGCCGTCATGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg                                                                                <  5:1679469/151‑1 (MQ=255)
                                                                  cAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCAGGAGTAATGAAGCCAAAACGttt                                                                                  >  2:276525/1‑148 (MQ=255)
                                                                   aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc                                                                              <  2:1066687/151‑1 (MQ=255)
                                                                    gCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACt                                                                             <  5:1576668/151‑1 (MQ=255)
                                                                      tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCg                                                                           >  1:815017/1‑151 (MQ=255)
                                                                       gCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc                                                                            <  7:825892/149‑1 (MQ=255)
                                                                       gCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGt                                                                          >  3:413170/1‑151 (MQ=255)
                                                                           ggccTTTCTGGCCGTCCTGAATTTCGCACTCCACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGCGAGCGGAGAAGTGTACGACCACATCTTTGCTGCCATCAGCCGGAGTAATGACGCCAACACGTTTAGACTCGTTGa                                                                       >  4:1691233/3‑150 (MQ=255)
                                                                           gACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGaa                                                                      <  3:1185898/151‑1 (MQ=255)
                                                                            aCCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc                                                                     >  6:334665/1‑151 (MQ=255)
                                                                            aCCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc                                                                     >  1:1062967/1‑151 (MQ=255)
                                                                            aCCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc                                                                     <  2:1181533/151‑1 (MQ=255)
                                                                                ttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCa                                                                   <  2:1401144/149‑1 (MQ=255)
                                                                                    ggCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACtt                                                                <  4:855661/148‑1 (MQ=255)
                                                                                    ggCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTaa                                                              <  7:150221/150‑1 (MQ=255)
                                                                                    ggCCGTCCTGAATTTCGAACTATACTTTCTTAACTTGATGCAGACTTTTGAAGCGATTACCCTAGATAGCGGAGAAGTTTACGAACTCATCTTTGCTGGCATCATCCTGAGTAATGAAGCCAAAAGCTTTAGACTCGTTGAACCACTTaa                                                              <  4:953170/150‑1 (MQ=255)
                                                                                         tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc                                                        >  1:161357/1‑151 (MQ=255)
                                                                                          ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAcc                                                       >  1:1522416/1‑151 (MQ=255)
                                                                                             gAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt                                                      <  6:1049767/149‑1 (MQ=255)
                                                                                             gAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt                                                      <  6:72427/149‑1 (MQ=255)
                                                                                               aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa                                                  <  5:197020/151‑1 (MQ=255)
                                                                                                tttAGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAACCCATTACCCTAGATAGCGGAGAAGTGTAGGAAAAAATCGTTGCTGACATCAGCCGGAGTAATGAAGCAAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAAt                                                 >  6:866540/1‑151 (MQ=255)
                                                                                                   cGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCtt                                              >  1:1741449/1‑151 (MQ=255)
                                                                                                    gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAACCTAGATAGCGGAGAAGTGTACGAACACATCTATGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt                                             >  4:1028531/1‑151 (MQ=255)
                                                                                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTg                                            >  3:1411415/1‑151 (MQ=255)
                                                                                                         cAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                        >  3:574819/1‑151 (MQ=255)
                                                                                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt                                       <  1:1184510/151‑1 (MQ=255)
                                                                                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt                                       >  1:451183/1‑151 (MQ=255)
                                                                                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt                                       <  2:626067/151‑1 (MQ=255)
                                                                                                            cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTg                                     <  7:438732/151‑1 (MQ=255)
                                                                                                            cGTTATGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTg                                     >  7:1324948/1‑151 (MQ=255)
                                                                                                             gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTg                                     >  6:1405914/1‑150 (MQ=255)
                                                                                                               tCTGCCCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaa                                   >  4:493332/1‑150 (MQ=255)
                                                                                                                 tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaa                                 >  1:641880/1‑150 (MQ=255)
                                                                                                                  gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa                                <  6:1787625/150‑1 (MQ=255)
                                                                                                                  gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa                                <  3:1316670/150‑1 (MQ=255)
                                                                                                                  gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaa                                 <  8:849030/149‑1 (MQ=255)
                                                                                                                  gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAt                               <  2:1694882/151‑1 (MQ=255)
                                                                                                                   aaatttaGCCAGAGTTTTGAAGCCATTACCCTATATAGCGGAGAATTGGACGAACACATCTTTGCTTCCATCAGCCGTAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTACCCTGACCTTTAATCTTTGACATTTGAAAAAtt                              <  1:910407/145‑1 (MQ=255)
                                                                                                                    ccTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTc                             <  7:1447583/151‑1 (MQ=255)
                                                                                                                     cTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc                            >  5:744162/1‑151 (MQ=255)
                                                                                                                         aGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCtt                          >  4:1568522/1‑149 (MQ=255)
                                                                                                                          gCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCtt                          >  3:707685/1‑148 (MQ=255)
                                                                                                                          gCCAGAGTTTTGAAGACATTCCGCTAGATTGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCATAACCTTTAGACTCGTTGAACAACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGa                       <  7:325586/151‑1 (MQ=255)
                                                                                                                           ccAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCtt                          >  2:502717/1‑147 (MQ=255)
                                                                                                                           ccAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGAt                      <  1:330667/151‑1 (MQ=255)
                                                                                                                             agagTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTg                    <  8:614445/151‑1 (MQ=255)
                                                                                                                                gTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGttt                 <  5:171822/151‑1 (MQ=255)
                                                                                                                                  tttGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtttt                <  3:925222/150‑1 (MQ=255)
                                                                                                                                  tttGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttc               >  6:629515/1‑151 (MQ=255)
                                                                                                                                    tGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttctt             >  7:1781103/1‑151 (MQ=255)
                                                                                                                                     gAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttctt             <  6:1531842/150‑1 (MQ=255)
                                                                                                                                      aaGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCg           <  8:1522755/151‑1 (MQ=255)
                                                                                                                                       cgCCATTACCCTAGATAGCGGCGAAGTGGACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGACGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTCATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGc          <  5:596837/150‑1 (MQ=255)
                                                                                                                                         ccATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGccc        <  4:1442595/151‑1 (MQ=255)
                                                                                                                                             tACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCa     <  1:1422852/150‑1 (MQ=255)
                                                                                                                                               cccTAGATAGCGGAGAAGTGTACGATCACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGc  >  5:942800/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGC  >  NC_000913/1907180‑1907473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: