Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG30.7% 572.4 / 98.5 357intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (62/182);  new base G (105/3);  total (172/185)
Fisher's exact test for biased strand distribution p-value = 5.65e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579
                                                                                                                                                     |                                                                                                                                                       
     gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGtgctccg                                                                                                                                                   >  5:1373451/1‑150 (MQ=255)
     gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                                   <  6:50657/151‑1 (MQ=255)
     gcgTGGAGTGTTGGGCTGTTGAGTGGCTGGATGTTTAAACGCCGCCACGATCGGCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                                   <  1:96777/151‑1 (MQ=255)
       gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCtccgcctcc                                                                                                                                                 >  2:984639/1‑151 (MQ=255)
        tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgccgcgtgcc                                                                                                                                                >  7:370308/1‑144 (MQ=255)
         ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGcccc                                                                                                                                               >  4:863081/1‑151 (MQ=255)
              gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCcaa                                                                                                                                           >  8:1403169/1‑150 (MQ=255)
                 gtgCTGTGTATTGGCTGGAGGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGCACATCCCGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAatat                                                                                                                                        <  2:1223228/150‑1 (MQ=255)
                   gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                      <  8:1015430/150‑1 (MQ=255)
                    cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGTATGAAAACCAGTAATCCTTGCGTTCAGTAATCAGGTTAAGAAGGAAATCCTGATCCGGCTATTCCGAAAGATATCCGCCCCCGACCGCCTCCACCAAAAATCCtg                                                                                                                                    >  6:1356788/1‑150 (MQ=255)
                     tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGTCCGGGGCCCCCCAATATccgt                                                                                                                                    >  6:579859/1‑148 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCGGCCGCCCCCACAGATCCTTcc                                                                                                                                  >  3:1060939/1‑150 (MQ=255)
                          tttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                               <  1:34963/149‑1 (MQ=255)
                          aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTGCTCCTCCCCCAAATATCGTTGAgctg                                                                                                                              >  7:1490203/1‑151 (MQ=255)
                          aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatg                                                                                                                              <  5:1183971/151‑1 (MQ=255)
                          aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatg                                                                                                                              <  7:810584/151‑1 (MQ=255)
                            tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGGCCCCCCAATATACGTCAgatgat                                                                                                                            >  1:1602586/1‑151 (MQ=255)
                              gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                          <  5:242076/151‑1 (MQ=255)
                               cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCGCCCCCAAATATCCTTCAGatgatg                                                                                                                           >  6:795441/1‑149 (MQ=255)
                                 tgATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCATGAGGAAATCCTGATGCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATTATGAGt                                                                                                                        <  2:636159/149‑1 (MQ=255)
                                 ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCGCCCCCATATCCTTCAGATGATGAGTg                                                                                                                       >  3:1497443/1‑151 (MQ=255)
                                     gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCCTTCAGATGATGAGTGATcc                                                                                                                   >  8:624799/1‑151 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                  <  2:212199/150‑1 (MQ=255)
                                         aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCACTATCCTTCAGATGAGGAGTGTTCGTGcc                                                                                                               >  8:1224423/1‑150 (MQ=255)
                                         aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGATGATGAGTGCTCCTGcc                                                                                                               >  5:791080/1‑150 (MQ=255)
                                            cgccgcCTCGATCGTCTGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTGAGGAGGAAATCCTGATCCGGCTATTGCGAAAGTTATCCGCCCCCGTCGTCCTGCCCCAAATATCCTTCTGATTATGAGTGATCCTGCATTa                                                                                                            <  4:1298665/151‑1 (MQ=255)
                                            cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                            <  4:1434613/151‑1 (MQ=255)
                                              ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCGCCCCCCAATATCCTTCAGATGATGAGGGATGCTGGCTTAgg                                                                                                          >  1:894533/1‑151 (MQ=255)
                                                gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTCCGCCCCCAAAAATACTTCAGATGATGAGTGATCCTGCAGTAGGCt                                                                                                        >  6:1262807/1‑151 (MQ=255)
                                                gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGGCTCCCCCCCGTATCCTTCAGATGCTGAGTGATCCTGCATTAgggt                                                                                                        >  3:167204/1‑149 (MQ=255)
                                                gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGCTCCTGCATTAGGCt                                                                                                        >  8:1290549/1‑151 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                     <  7:587034/151‑1 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCAGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATCCTTCAGATGATGAGTGGTCCTGGATTATGGTATg                                                                                                     >  4:444105/1‑151 (MQ=255)
                                                     gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCTCCCCCAAAAAAGCTTCAGATGAGGAGGGATCGAGCAATAGGCtggtgc                                                                                                   >  6:741944/1‑146 (MQ=255)
                                                     gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCTCGCCCCCCTATCCTTCAGATGATGAGTGTTCCTGCAGTAGGAGATGGc                                                                                                   >  7:1415111/1‑151 (MQ=255)
                                                         gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                               <  3:859518/151‑1 (MQ=255)
                                                         gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCCAATAACCTTCAGATGATGAGTGATCCTGCATTAGGGTTTGGCATgg                                                                                               >  6:938133/1‑151 (MQ=255)
                                                            aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGGTCCTGCATTTGGCTATGGCAAGGTgt                                                                                            >  7:1136633/1‑150 (MQ=255)
                                                                   tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGACAACCCCAAAAAACCTTCAAATGATGAGTGATAATGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                     >  1:545363/1‑151 (MQ=255)
                                                                     aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGGCTGCGCGCCCAAAGAGCGTTCAGATGAGGAGGGATGGGGGAGTAGGCTAGGGCAAGGTGGTCGGAtttt                                                                                   >  6:1444758/1‑151 (MQ=255)
                                                                     aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCTTTAGGGTATGGCAAGGTGAGGAGCtttt                                                                                   >  3:958735/1‑151 (MQ=255)
                                                                       aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                 <  5:336323/151‑1 (MQ=255)
                                                                        aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGAGCTACTCCCCCAAATATCCTTAAGATGATGAGTGTTCGTGCATTAGGCGAAGGCAAGGCGCTCAGATTTtca                                                                                 >  4:603022/1‑150 (MQ=255)
                                                                         ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATGCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                               <  6:1608106/151‑1 (MQ=255)
                                                                         ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGCGCGCCTCCCCCAAATATCCTTCAGATCATGAGTGATCCTGCCATAGGCTATGGCAAGGGGATCGGAGTTtcatc                                                                               >  1:244077/1‑151 (MQ=255)
                                                                            gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                            <  1:690136/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                          <  7:185644/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                           <  7:1155884/150‑1 (MQ=255)
                                                                                    tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGGCGCCGGCCCCAAAGAGGCTGCAGATGATGAGTGATCCTGCATTAGGGTATGGGAAGGTGATGAGAGTTTCTTCAGAGGGGAttt                                                                    >  4:504186/1‑151 (MQ=255)
                                                                                     gCGTTGTGTAAGCCGGTTCAGGATGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCTCCTCCCGCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATGACAGTGGAAtt                                                                    <  4:1562467/150‑1 (MQ=255)
                                                                                     gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGGCTCCCCCAAATATCCGTCCGATGATGGGTGATGCTGCATTAGGGTATGGGAAGGTGCTCAGCTTTTCATCACAGGGGAAttt                                                                   >  5:1623910/1‑150 (MQ=255)
                                                                                       gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGCCACCTCCCCCCAATAACAATAAGATGAAGAGTGATCCTGCATTAGCCTATGGCAAGGGGATAAGATTTTCATAACAGGGGAATTatt                                                                 >  4:1107174/1‑150 (MQ=255)
                                                                                         tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGAGGAGTGATACTTCATAAGGCTATGGCAAGGTGATCAGAATTTGATAAGAGGGGAATTttgat                                                               >  6:1031346/1‑151 (MQ=255)
                                                                                           tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCATTCAGATGATGAGTGATCCTGCATTAGGCGATGGCGAGGGGATCATATTTTAATCAAAGGCGAATTatgattt                                                             >  4:36422/1‑149 (MQ=255)
                                                                                           tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATAATTCAGATGATGAGTGATACTGCATTAGGCAATGGCAAGGTGATCAGATTTTGATCAAAGGGGAATAATTATGt                                                             >  1:1482666/1‑151 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCGGGCCTCGGCCCGCAAGTATCCTTCAGAGGATGCGGGGTCGTGCATTAGGGGATGGCAGGGGGGGGTGAGTTTGATGACTGGGGGATTatgtggtt                                                            >  6:1171354/1‑146 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                               <  7:682628/148‑1 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                               <  1:48661/148‑1 (MQ=255)
                                                                                               aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAAAACTACAGATGATGAGTGATCCTGCATTAGTCTATGGGAAGGTGAGCAGATTTTGATCAAAGGGGAAGTATTATGTTaa                                                          >  1:1102280/1‑150 (MQ=255)
                                                                                                gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATACTTCAGATGATGAGTGATGCTGGATTAGGCTATGGCAAGGTGATCAGATTTTGATCAAAGGGGTATTATGATTTTTAAt                                                        >  4:165008/1‑151 (MQ=255)
                                                                                                   gggtCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                     <  7:1198007/148‑1 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                      >  5:430322/1‑150 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                     <  8:1659442/151‑1 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                     <  1:1063615/151‑1 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCAGTCAGAAGATGAGGGATGCTGCATTAGGCTAGGGCAAGGTGAAGAGATTTTCATCACCGGGGTATTATGATGTTAAATga                                                      >  8:1355713/1‑149 (MQ=255)
                                                                                                      tCAGGAGGAAATCCTGATCCGTCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                   <  4:94207/150‑1 (MQ=255)
                                                                                                        aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGGCGGGGCCCCCCCATATCAGGCCGATGATGAGTGCTCCGGCATTAGGCTATGGCAAGGTGATGAGCTTTTAATCACAGGGGAATTATTATGTTATATGCAttga                                                 >  7:332066/1‑147 (MQ=255)
                                                                                                            ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCTCGTCCCCCAAATATACAGCAGAGGAAGAGTGATGCGGAATTAGGGTATGGCAAGGTGTACAGATTTTAAAGACACGGGAATTATGTTATTAAATTAATggaagc                                              >  2:251700/1‑145 (MQ=255)
                                                                                                             gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTATGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATTTTAAATGAATGGCACCTG‑c                                          >  4:667362/1‑151 (MQ=255)
                                                                                                              aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑cc                                         <  3:138892/151‑1 (MQ=255)
                                                                                                              aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑cc                                         <  1:1398094/151‑1 (MQ=255)
                                                                                                                     gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCATATATCGTTCAGATGATGAGTGATCCTGAATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGcc                                   <  7:1027598/150‑1 (MQ=255)
                                                                                                                     gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGccc                                  <  3:999834/151‑1 (MQ=255)
                                                                                                                      aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGcccc                                 <  8:349048/151‑1 (MQ=255)
                                                                                                                       tCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGcccc                                 <  2:1361952/150‑1 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGccccc                                <  6:851002/150‑1 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCGTGCATTAGGCTATGGCAAGGTGATGAGATTTTGGTCACAGGGGGATTATGATGTTTAATGCATGGCACCGG‑CCGGGGcggccc                               >  4:1224267/1‑146 (MQ=255)
                                                                                                                           gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCAttt                            <  8:765792/151‑1 (MQ=255)
                                                                                                                             tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATACGGCATTAGGCTATGGCAAGGTGATAAGATTTTGATCTCAGGGGAATTTTGATGTTAAATTCATGGCACCTGCCCGGGGccccctgtgt                           >  1:744842/1‑146 (MQ=255)
                                                                                                                             tATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATACGTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCAAAGGGGAATTATGATGTTTAATTCATGGCACCTG‑CCGGTGCGCCCCGTTGt                          >  2:913306/1‑151 (MQ=255)
                                                                                                                              aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTg                           <  6:40259/149‑1 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTaaa                      <  5:1317848/151‑1 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTaaa                      <  3:1548637/151‑1 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATAAGATTTTGATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGGCCCCATTTGTTaa                       >  1:1030331/1‑150 (MQ=255)
                                                                                                                                  cGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAATATCCTTAATATGATGAGTGATGCGGCAGTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTAAGATGTTTAATGCTAGGAACCTG‑CCGCGGGCCCCAGATGTTaaa                      >  2:511378/1‑150 (MQ=255)
                                                                                                                                     aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAAcaaa                  <  8:835011/151‑1 (MQ=255)
                                                                                                                                       gTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGATGATGAGTGATCCGGCATTAGGCTATGGGAAGGTGCTGAGATTTTCATCACAGGGGAATTATGATGTTTAATGCATGGCCCCGG‑CCGGGGCGCCCATTTGTTAAAcaaagc                >  2:159154/1‑151 (MQ=255)
                                                                                                                                        ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAAcaaagc                <  2:1489450/150‑1 (MQ=255)
                                                                                                                                         tATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTTATCCTGCATTATGCTATGGCAAGGTTATAACATTTTAATAAAAGGGGAATTATTATTTTAAAAGCATGGCACCTG‑CCCGTGGCCCCATTTTTTAAAaaaacca               >  3:711612/1‑150 (MQ=255)
                                                                                                                                            ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAAcaaagcaaag            <  5:565177/150‑1 (MQ=255)
                                                                                                                                            ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAAcaaagcaaag            <  1:1301173/150‑1 (MQ=255)
                                                                                                                                             cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt          <  7:893556/151‑1 (MQ=255)
                                                                                                                                               cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa        <  3:440829/151‑1 (MQ=255)
                                                                                                                                               cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa        <  7:1088499/151‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa       <  7:535781/151‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa        >  4:777536/1‑150 (MQ=255)
                                                                                                                                                 cccGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      <  8:1520751/151‑1 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  3:1252384/151‑1 (MQ=255)
                                                                                                                                                   cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    <  3:296479/151‑1 (MQ=255)
                                                                                                                                                   cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    <  5:1554102/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTG‑CCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑