Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,591	0	A	.	33.3%	160.8 / 85.4	69	intergenic (+18/‑16)	metW/metV	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (43/3);  new base . (22/1);  total (65/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

GGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947501‑2947740                                                                                             |                                                                                                                                                          gggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCgg                                                                                                                                       <  8:202708/111‑1 (MQ=255) gggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TA‑AAATTTGATTAAGGAAAAGCAGTACGGTGACGCgg                                                                                                                                       >  2:202356/1‑111 (MQ=255)      gAGCAGCCTGGTAGCTCGTCTGTCTTATAAACCGAAGGTCGTCGGTTCAAATCCTTCACTATCAACTAA‑TC‑AAATTTGATGCACTAATAGCATTAGTTTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                                          <  6:363295/151‑1 (MQ=11)                                           gtcgtcGGTTCAAATCCGGCCCCCGCAACCAA‑TA‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAgg                                                                                             <  5:164027/111‑1 (MQ=37)                                                 ggTTCAAATCCGGCCCCCGCAACCAA‑TG‑AAATTTCATGCAGTAAACGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTCGCTCGTCGGGCTCATAACGCGTAGCTCGGCGGGTCGCGT‑CCGGCCCCCGCCACCAATGATTTAACACCCtc                                              >  7:89170/1‑150 (MQ=14)                                                            ggCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTCGAGCAGCCTGGTCGCTCGTCGGGCTGATAACCCGAAGGGGGGCGGGTCCCCT‑CCGGCCCCCGCCACCAATTATTGAACACCCG‑AACGG‑ctg‑tgat                                >  5:1483028/1‑148 (MQ=17)                                                            ggCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGAAGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTAGGCGGTTCAACT‑CCGGCCCCCGCAACCAATTATTGAAAACCCT‑AACCG‑GTG‑tttt                                >  3:1167676/1‑151 (MQ=17)                                                             gCCCCCGCAACCAA‑TC‑AAATTTCATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑ttttt                               >  1:1623078/1‑151 (MQ=255)                                                                cccGCACCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCCACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTg                            >  5:658354/1‑151 (MQ=255)                                                                 ccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AAAGG‑GTG‑TTTTTTTGt                           >  6:220329/1‑151 (MQ=255)                                                                  cGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCCACCAATTATTGAACACCCT‑AACGC‑GTG‑TTTTTTTGtt                          >  3:575667/1‑151 (MQ=255)                                                                  cGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TGTTTTTGtt                          >  7:797481/1‑151 (MQ=255)                                                                   gCAACCAATTA‑AAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATGAATTAACAGCCA‑CACGG‑GTG‑TTTATTTggt                          >  8:1544177/1‑148 (MQ=11)                                                                   gCAACCAATTA‑AAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATAAATTAACAACCA‑AAAAC‑ATG‑TTTATTTGtt                          >  2:1669337/1‑150 (MQ=14)                                                                   gCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGttt                         >  8:8196/1‑151 (MQ=255)                                                                   cccccaacaaagaaaaTTGATGAATTAAAAGCAGTACGGTGACGCGGGGTGGAGCGCCCTAGTAGCGAGAAGGGCATATAACCCGAAGGAACTACGTTCAAATACCGGCCCCCTC‑CCCAATTATTGAACAACCA‑AACGG‑GTG‑GTTTTTTGt                           >  6:1606102/13‑151 (MQ=12)                                                                    cAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTc                        >  6:766882/1‑151 (MQ=255)                                                                     aaCCAA‑TTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGGAACCAATTATTTAACAACCA‑TAAGG‑GTGATTTTTTTGttcg                        >  4:1310615/1‑149 (MQ=11)                                                                     aaCCAA‑TTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTTAACAACAT‑GAAGG‑GTG‑TTTTATTTTTTCt                       >  4:433785/1‑151 (MQ=11)                                                                     aaCCAA‑TTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATGATTGAACACCCT‑CACGG‑GTG‑TGTTGTTGtttgt                       >  8:430894/1‑149 (MQ=11)                                                                     aaCCAA‑TTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATCAATGTACACGCTGAACGGTGTg‑tttgttttgct                         >  7:632241/1‑147 (MQ=14)                                                                     aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCt                       >  8:605735/1‑151 (MQ=255)                                                                     aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCt                       >  4:440433/1‑151 (MQ=255)                                                                      aCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTg                      >  4:1492968/1‑151 (MQ=255)                                                                       ccAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTgg                     >  1:1268348/1‑151 (MQ=255)                                                                       ccAA‑TC‑AAATTTGATGAAGTAAAAGCAGTAAGGTGACGCGGGGTGGAGAAGCCTGGTAGCTCGTCGGGGTCATAACCCGAAGGTCGTCTGTTCAAAT‑ACGGCGCCCGCAAGCAATTATTCAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTgg                     >  3:481902/1‑151 (MQ=255)                                                                       accaattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTAATGAACACCCT‑AAATC‑GTG‑attttttttatgtg                      >  5:521292/9‑144 (MQ=1)                                                                        ccaattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTgg                     >  8:1485894/8‑151 (MQ=255)                                                                        ccaattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATGATTGAACACCCT‑AAAGG‑GTG‑TGTTTTTGGTTCTgg                     >  8:1123284/8‑151 (MQ=14)                                                                        cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGt                    >  1:1324683/1‑151 (MQ=255)                                                                         caattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTTAACAACCT‑AACCG‑GGG‑TTTTTTTGTTTCAGGt                    >  6:192341/7‑151 (MQ=11)                                                                         caattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑TTCGG‑CTG‑TTTTTTTGTTTCTGGt                    >  8:215999/7‑151 (MQ=14)                                                                         aa‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGtc                   >  2:322155/1‑151 (MQ=255)                                                                          aattaaaaTTTGATGATGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑aa                                            <  2:1573984/123‑1 (MQ=255)                                                                           attaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATGATTGAACACGCT‑AACCG‑GGG‑TTTTTTTGTTTCTGGgct                  >  3:158637/5‑151 (MQ=14)                                                                           attaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATCATTTTACACCAA‑ATCGG‑GGC‑TTTTGTTGTTGCGGGtct                  >  7:301937/5‑151 (MQ=11)                                                                            ttaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTTAACACCCA‑AACGC‑GGG‑TTTTTTTGTTTCAGGtctc                 >  1:1035436/4‑151 (MQ=14)                                                                            tC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCACCCAATTATTGAACCCCAT‑AACGG‑GTG‑TTTTTTTGTTTCTGGtgtc                 >  1:491994/1‑151 (MQ=255)                                                                             taaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACTG‑GGG‑ATTTTTTGTTGCTGGGCTcc                >  2:183413/3‑151 (MQ=14)                                                                             c‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCTGTTAAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTcc                >  4:1399763/1‑151 (MQ=255)                                                                               aaaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTccc               >  5:880767/1‑151 (MQ=255)                                                                               aaaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTccc               >  7:1589292/1‑151 (MQ=255)                                                                                aaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTccc               >  8:271041/1‑150 (MQ=255)                                                                                aaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTccc               >  6:1639147/1‑150 (MQ=255)                                                                                aaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTccc               >  7:882869/1‑150 (MQ=255)                                                                                 aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGCGCAGCGTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCACCCAATTATTGAACACCCT‑AACGG‑GGG‑TTTTTTTGTTTCTGGTCTccat              >  5:140307/1‑148 (MQ=255)                                                                                 aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCAt             >  8:887389/1‑151 (MQ=255)                                                                                 aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCAt             >  7:408603/1‑151 (MQ=255)                                                                                 aTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCA‑AACGA‑GTC‑ATTAATTGGTGCTGGTCTCCCAt             >  4:1345205/1‑150 (MQ=14)                                                                                    tgatgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaa           >  2:1055779/1‑150 (MQ=255)                                                                                    tgatgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaa           >  2:1305789/1‑150 (MQ=255)                                                                                    tgatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCAT‑AAAGC‑GTG‑TGTTGTTGTTTCTGGTCTCCCATaaa          >  7:1300656/1‑150 (MQ=17)                                                                                     gatgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaaa          >  7:1255672/1‑150 (MQ=255)                                                                                     gatgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaaa          >  3:1017874/1‑150 (MQ=255)                                                                                      atgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaaaa         >  5:680918/1‑150 (MQ=255)                                                                                      atgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaaaa         >  2:1389131/1‑150 (MQ=255)                                                                                      atgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACAACCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaaaaa        >  4:429467/1‑150 (MQ=255)                                                                                      atgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTAATGAACACCCT‑AAAGG‑GTG‑TTTTTTTGTTAGTGGTCTCCCAaaaaaa        >  2:781615/1‑150 (MQ=17)                                                                                      atgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATAATTGAACACCCT‑ATAAG‑CTG‑TTTTGTTGTTTCTGGTCTCCCATaaaaac       >  3:1188498/1‑150 (MQ=17)                                                                                       tgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaaaaa        >  7:693184/1‑150 (MQ=255)                                                                                       tgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATaaaaa        >  5:21254/1‑150 (MQ=255)                                                                                       tgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTTTTTCTGGTCTCCCAGaaaaacg      >  7:62382/1‑149 (MQ=255)                                                                                       tgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATGAATGAACACGCT‑AAAGG‑GGG‑TGTTGTTGGTTGTGGTGTGCCTTaaccacg      >  8:172189/1‑146 (MQ=11)                                                                                         aaGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAgc     >  4:749337/1‑151 (MQ=255)                                                                                         aaGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACACGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCCACCAATTATTGAACACCCT‑AACGC‑GGG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAgc     >  1:431986/1‑151 (MQ=255)                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  2:913467/1‑151 (MQ=255)                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  4:1137110/1‑151 (MQ=255)                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  3:267543/1‑151 (MQ=255)                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  5:1234535/1‑151 (MQ=255)                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  8:1187779/1‑150 (MQ=255)                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  1:1385579/1‑150 (MQ=255)                                                                                             |                                                                                                                                                          GGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAT‑CCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGG‑GTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947501‑2947740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑