Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1510AC27.1% 159.5 / 28.9 172intergenic (+549/+474)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base A (78/43);  new base C (44/1);  total (127/45)
Fisher's exact test for biased strand distribution p-value = 2.36e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270006‑3270301
                                                                                                                                                 |                                                                                                                                                             
                             tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCTTGGCCAGTCAGTC‑AATTTTGA‑CCCCCGCCAAAACC‑aa                                                                                                                           >  3:928663/1‑150 (MQ=11)
                               gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGCTAAGCCGCTTTCTTTTGGGTATAGCGTCGGGGCAAGGCAATC‑ATGTTTGC‑GCCCCCGCCACAGC‑aaaa                                                                                                                         >  2:635597/1‑150 (MQ=11)
                                  aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTA‑ATATTTGT‑GCGCCCCCAAAAGC‑AAAAAacc                                                                                                                     >  7:82606/1‑151 (MQ=11)
                                         catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGGCATTC‑ATAGTTGC‑TCGCCGCCAATACC‑AAAAACccggaga                                                                                                                >  7:758284/1‑145 (MQ=11)
                                         catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGAGTCGTGTCTAGTCAATC‑AATGTTGG‑CCGCCCTCAAAAAC‑AAAAACccgcggc                                                                                                                >  8:950266/1‑146 (MQ=11)
                                            cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGGGGCCAGGCATGC‑ATGGTTGG‑GCCCCGGCAAAACC‑AAAAACCCGCCG‑CA‑G‑ccg                                                                                                        >  3:1175114/1‑149 (MQ=11)
                                                 tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGGGATTC‑ATGGTTGG‑GGCCCGACAAAAGC‑CAAAACCCGCCG‑CA‑G‑C‑cgggggt                                                                                                  >  5:1600539/1‑147 (MQ=11)
                                                    cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTGCTTC‑ATGGTTGT‑GCCGCGGCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GGGtgttttc                                                                                               >  5:714429/1‑149 (MQ=11)
                                                      cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGAGTATAGGAAAGTCATTC‑AAGATAAA‑ACACCAACAAAAAC‑AAAAACCCGCAG‑AA‑gaacggttata                                                                                                  >  4:1474430/1‑138 (MQ=2)
                                                       tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTGT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTTCGt                                                                                             >  2:59541/1‑150 (MQ=25)
                                                       cccACAAGCACACACCCGCCGCAGCGGGTTATTACGTAAAACCGGTAAAACTGACCGATAAGCCGCATTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTa                                                                                            <  8:555695/150‑1 (MQ=11)
                                                        ccAACAGCAACAACCCCCCGAAGCGGGTTTTTACGTAAACCACGTGAACCTGACCGATAACCCGCTTGCATTTGGGTACAGGCGCGGCGACAGGCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTaa                                                                                           <  5:1109738/151‑1 (MQ=11)
                                                          aactcgataatcccGCCGACCCGTGTTCTCACGCCAACTAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAAt                                                                                         <  8:650480/140‑1 (MQ=11)
                                                           acccgaacaccccGCCGCAGCGGGTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATATTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATc                                                                                        <  8:1178454/141‑1 (MQ=11)
                                                           aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTAGACAGTCAATA‑ATGATTCA‑GCACCTACAAAAAC‑AAAAACCCGCAG‑CA‑G‑A‑GGGTTATTACCAAAAt                                                                                         >  1:320233/1‑150 (MQ=11)
                                                           aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTGGTGGCCAGGCATTC‑ATAGTTGG‑GCCCCGGCAAAAAC‑AAAACACCGCCG‑CA‑G‑g‑ggggtggttcggtaatc                                                                                        >  3:1612724/1‑137 (MQ=11)
                                                             atcataaaCCCGCCGGACCGGGTTATTACGTACACCAGGTCAAACTGACCGCTAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAg                                                                                      <  5:807840/146‑1 (MQ=11)
                                                               cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATGTTTCT‑GCCCCGCCAAAACC‑AAAAACCCGCCG‑CA‑G‑G‑GGGGGTTTACGTAAATCAggg                                                                                    >  7:1529529/1‑150 (MQ=11)
                                                                caacccccGCCGACGCGCGGAATTACGTAAACCAGCTGAACCTGACCTATAAGCCGCATTCGTTTGCGTAGACCGTCGTCGCGAGTGACTC‑CACTTGCT‑GCCCCTCCAAAAGC‑TAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTg                                                                                   <  3:136213/146‑1 (MQ=2)
                                                                acccatccgccgCACCGGGTTATTACGTACACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAgg                                                                                     <  5:943433/143‑1 (MQ=11)
                                                                aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATGGTTGT‑GCCGCTGCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GGGGGGTTTCGGAAATCAGGTg                                                                                   >  5:1255856/1‑151 (MQ=11)
                                                                 attccgatccgcaccGGTTTATTACGTACACCAGGTTAAACTGACTTATAATTATCTTTATTTTGTGTATAGAGTCGTTGACATTCATTT‑ATCTTTTT‑TCCCATCAATAAGC‑TAAAACGATCCG‑TA‑T‑C‑GGGTTTTTACGTAAATCAGGTGa                                                                                  <  4:80062/137‑1 (MQ=255)
                                                                 aaaccgctccgcaccGGGTTATTACGTAAATCAGGTAAAACTGACGGATAATAATCTTTCTTTTGGGTATAGAGTCGTTGACATTCATTC‑ATCTTTTT‑GCCCCTCCAAAAGC‑AAAAACGAGCCG‑AA‑T‑C‑GGGTTTTTACGTAAATCAGGTGa                                                                                  <  1:1364809/137‑1 (MQ=255)
                                                                  aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGCAGTC‑ATAGTGGG‑GCAGCGACAAAACC‑AAAAAACCGCCG‑AA‑G‑C‑GCGTTTTTAAGGTAATCAAGTgc                                                                                  >  7:1399709/1‑149 (MQ=2)
                                                                  aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTAGAGCGTCGTGGGCAGTCATTCAATGTTTGT‑‑CCCCCGCAAAAAC‑CAAAACCCGCCC‑AA‑G‑C‑CCGGTTTTTCGTAAAtaaccgcc                                                                                  >  5:1547244/1‑143 (MQ=2)
                                                                    aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTGG‑GCGCCTGCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GGGGTTTTACGTAAATCAGGTGAAAc                                                                               >  3:954104/1‑151 (MQ=11)
                                                                       cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATGGTTGT‑GCCCCGCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑CGGGTTTTAAGTAAATCAGGTGCAACTgc                                                                            >  5:1331519/1‑150 (MQ=11)
                                                                        tacttatagggTTTTTACGTATAGCATGTGAAACTGATACATTATTTGCTTTCTTTTGGGTATTGCGTTGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGa                                                                            <  1:1583046/142‑1 (MQ=11)
                                                                          cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGCAAGTCAATC‑AAAGTGAA‑GCACCAACAAAAAC‑AAAAACCCGCCG‑AA‑G‑A‑CCGTTTTTTCCTAAATGACGTGAAAATGAccc                                                                         >  2:460276/1‑150 (MQ=2)
                                                                          cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGATAGCGTCGAGGCCAGGCATTC‑AACGTTGA‑GCCCCAGCAAACAC‑AAAAACCCGCCG‑CA‑G‑C‑CGGGGGGTAAGGAAATCAGGGGAAACTGcccc                                                                         >  6:677217/1‑150 (MQ=2)
                                                                          cgAAGCGGGTTTTTACGTAAACAAGGTGAAACTGACAGATAAGCATCTTTCTGTTGTGTATAGAGTCGTGGCCAGACATTA‑AACTGTTT‑GCACCGACAAAAGC‑AAAAAACCGCCG‑CA‑G‑C‑CGGGGTGTACGGAATTCAAGTTAAACttcccc                                                                         >  7:791055/1‑146 (MQ=2)
                                                                           gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTGT‑GCCCCGCCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑CGGGGTTTAAGTAAATGAAGTGAAACTTACCgc                                                                        >  3:1069725/1‑150 (MQ=11)
                                                                             aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTGGTGGAAAGTCAATC‑ATGATTTT‑ACACCATCAAAAAC‑AAAAACCCGCGG‑CA‑G‑A‑CCGTTTTTAAGGAAATGAGGGGAAAATTACCCAt                                                                       >  4:1340307/1‑150 (MQ=2)
                                                                               ccgcttcctACCTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTTTTTTATTGTTCTGGACAGTAATTA‑TTTTTTCT‑GTATCTCCAAAAGT‑ATTTACCTCCAG‑CA‑G‑G‑GTGTTTTTATGTAAATCAGGTGAAACTGACCGATAAg                                                                    <  2:732341/143‑1 (MQ=2)
                                                                               cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATGTTTGT‑GCGCCTGCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GGGGTTTTAAGTTAATCAGGGGAAACTTAGCGATaat                                                                    >  3:914880/1‑150 (MQ=11)
                                                                                gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTGCATC‑ATCGTTGT‑TCCCCCCCAAAACC‑AAAAAACCCCCGCAC‑G‑G‑GGGTTTTTA‑GTAAAACAGGTGCAACAGCCCGAGaaac                                                                   >  7:373816/1‑149 (MQ=2)
                                                                                 ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATC‑ATCTTTAG‑GCCCCAACAAAAAC‑AAAAACCCGCCG‑AA‑G‑A‑GGGTTATTACGTAAATCAGGTTAAACTTACCGATAATcc                                                                  >  4:59682/1‑151 (MQ=11)
                                                                                  gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTGGAGGAGAGTCATTC‑ATGTTAGA‑GCGGCAGCAAAAAC‑AATAACCCGCCG‑CA‑G‑G‑GGGGGTTTAAGTAAATGAGGTGAAAATGACCGATAATCCg                                                                 >  2:234615/1‑151 (MQ=2)
                                                                                     tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGCCAGTCAATC‑ATCATTGA‑GCACCAACAAAAAC‑AAAAACCCGCAG‑CA‑GAC‑CGGGTTTT‑CCTAAATCAAGGGAAAATTCCCCATAATCCGGGt                                                              >  2:318895/1‑151 (MQ=2)
                                                                                       tACGTACATCAGGTCAAACTGACCGATAAGCCGCTTTTTTTTTTTTATATCGTCGTGGACAGTCATTA‑ATCTTTAT‑GCCAATACAAAATC‑AAAAAACGTCGG‑TA‑T‑T‑GGGTTTTTACGTAAATCAGGTTAAACTGACCGATa                                                                      <  6:146690/141‑1 (MQ=2)
                                                                                       tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATGTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GGGGTTTTACGTAAATCAGGTGAAACTTCCCGATGAGCCGGGttt                                                            >  5:1100097/1‑150 (MQ=14)
                                                                                       tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATAGTTTG‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GCGGTTTTACCGAAATCAGGGGCAACTGACCGCTAAGCCGGGtt                                                             >  5:390418/1‑150 (MQ=14)
                                                                                         cGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATC‑ATCATTCG‑GCCCCAACAAAAAC‑AAAAACCCGCAG‑AA‑G‑C‑CGGGTTTTACCGAAATGAGGTGAAACTTACCCGTAAGCCGGGTTCTg                                                          >  6:1593069/1‑151 (MQ=14)
                                                                                          gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATAGTTCT‑GCCCCGCCAAAACC‑AAAAACCCGCCG‑AA‑G‑C‑GGGGTTTTACGTAAATCAGGGGCAACTTCCCGATAAGCCGGGTTCTGt                                                         >  8:434234/1‑151 (MQ=14)
                                                                                          gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAATA‑AACATTGA‑GCGCCAACAAAACC‑AAAAAACCGCCG‑AACC‑C‑GCGTTTTAACG‑AAATCACCTCAAACAGCCCGATAAACCCGGTTCAGt                                                         >  3:988393/1‑151 (MQ=9)
                                                                                              aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTGGCCCGACATTA‑TTGTTTAA‑GGCCCCACAAAAGC‑AAAAACCCCCCC‑GA‑T‑C‑TGGTGTTTAAGAATATGAGGTGAAACTTACCAATAAGCCCCGGTCTGTAGTg                                                     >  1:487281/1‑151 (MQ=2)
                                                                                               ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATGATTGG‑GCCCCAACAAAAAC‑AAAAACCCGCAG‑AA‑G‑G‑CGGGGGTTAAGTAAATCACGGGAAACTGCCCCATTAGCCGGGTTCAGTctagg                                                    >  7:502872/1‑147 (MQ=9)
                                                                                                cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAATC‑ATATTTTG‑ACCCCAGCAAAAAC‑AAAAAACCGCAG‑AA‑G‑A‑GCGTTTTTAAGTAAATAAAGTGAAAGTTTCCCATAAGCCGGGTTCTGtatagg                                                    >  6:143377/1‑145 (MQ=9)
                                                                                                 aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTGGTGTAAAGTCAATC‑ATGATTGG‑GCCCCACCAAAAGC‑AAAAACCCGCCG‑CA‑G‑CGGGGTTGTTCCGT‑AATGAGGGGAAAATGCCCGATAAGCCGGGTTCTGttgggggt                                                  >  8:535457/1‑144 (MQ=2)
                                                                                                  ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATC‑AAATTTCT‑GCCCCGACAAAAGC‑AAAAACCCGCCG‑CA‑G‑A‑GGGGTGTTAAGAAAATCACGTGAAAATGACCCATAAGCCGGGTTCTGtgggcgggc                                                 >  1:954452/1‑143 (MQ=14)
                                                                                                  ggTGAAACTCACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTC‑AACTTTCG‑GCCCCCCCAAAACC‑AAAAAACAGCCG‑AA‑G‑A‑CCGTTTTTTAGTTAATGAGGTCCAACTGGCCGAAAAGCCGGGATGTGTGTTGGAcc                                                 >  8:213394/1‑150 (MQ=2)
                                                                                                   gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCGAGTCCATC‑ATGTTTGT‑GCACCAGCAAAAAC‑AAAAACCCGCAG‑CA‑GAC‑CGGTTTTTCCGT‑AATCACGGGCTAATGACCGATGAGCCGGGttgttttggggtttc                                                >  5:491123/1‑138 (MQ=2)
                                                                                                     taaactttaggATAATCTGTTTTCTTTTTGGTATATCTTCGTTTATAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt                                               <  4:450953/140‑1 (MQ=17)
                                                                                                     gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGTACAGTAATTA‑ATATTTAA‑AAACATACAAAATC‑AAAAAAACCCCG‑AA‑A‑A‑CGATTATTAAGTAAATCAGGTGAAAATTACCCATAAGCCGGGttattgagtggagagg                                               >  6:843255/1‑136 (MQ=2)
                                                                                                     aaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGTCGAGTGCTTG‑ATATGTCTGGGCGGACCAAAAGA‑AAAAACCGGCC‑‑AA‑C‑G‑GGGTTTTTGGGTAAATCTTGTGAAAAAGAACGAGAAGGCGGGgtgtgtgggggggcggg                                              >  5:1029719/2‑140 (MQ=2)
                                                                                                        aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCTTC‑ATCTTTGT‑GGCCCACCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GGGGTTTTAAGGAAATCACGTGAAACTGACCCATAATCCGGGTTCTGTCGGGGCCCGtgatt                                           >  7:712129/1‑151 (MQ=14)
                                                                                                        aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTGT‑GCCCCTCCAAAAAC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTAAGTAAATGAGGTGAAACTTACCGATAAGCCGGGTTCTGTCGTGGACAGtcatt                                           >  5:197617/1‑151 (MQ=255)
                                                                                                         cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGACC‑AA‑G‑G‑GGGGTTTTACGTAAATCACGTGAAAAAGACAGATAATGCTGGTTCTGTCTTGGATAGtgattt                                          >  8:132443/1‑150 (MQ=14)
                                                                                                           tttcgttaatcttttttctttTTGGACTAGCGTCGTTTTTAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GTGTTTTTACGTAAATCAGGTGAAACTGACGGATAAGACGGGTTCTGTCGTGGACAGtcattca                                         <  2:1059898/136‑1 (MQ=17)
                                                                                                           ccgcggctctgccgattctttTGGGTAAAGCGTCGTGGACAGTCATTG‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAATCGCGCCT‑AA‑G‑C‑GGTTTTTTACTTAAATCAGGTGAAACTGACCGATATGCCGGGTTCTGTCGTGGACAGtcattcat                                        <  8:313733/136‑1 (MQ=17)
                                                                                                            ccttcccaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACTTTCATTC‑ATCTTTAT‑GCCCCTCCAAAAGC‑AAAAACCCGCTG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                                       <  8:1513404/144‑1 (MQ=17)
                                                                                                            aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTAT‑GACCCGCCAAAAGC‑AAAAAACCCCCG‑AA‑G‑C‑GGGTTTTTATGTAAAAAACGTGAAAATGACACATAAGACGCGTTCAGTCGTGGACacaccatcat                                        >  3:342398/1‑141 (MQ=11)
                                                                                                             ccGATAAGCCTCTTTCTTTTGTGTATATTTTCGTTTACATTCATTC‑ATCTTTCT‑TCACCTACAAAATT‑AAAAACCCTCTT‑AA‑G‑C‑TTGTTTTTACTTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                                      <  1:1027316/151‑1 (MQ=12)
                                                                                                             ccGATAAGCCGCTTTCTTTTGTGTATAGTTTTTTTTAGAGTCATTC‑ATCTTTCT‑TCACCTACAAAATA‑TAAAACCCTCTT‑TA‑G‑C‑TTGTTTTTTCTTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                                      <  1:1035120/151‑1 (MQ=12)
                                                                                                             ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGGCAATG‑ATCTTTGT‑GCCCCGCCAAAAACAAAAAACCCCCCC‑AG‑C‑C‑GGGTTTTTAGTTAAAAAAGG‑GAAAAATACCGATAATCCGGGTTGTGTGGTGGGCAGtgattcatat                                      >  7:1200278/1‑149 (MQ=9)
                                                                                                              cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTG‑ATCTTTGT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTGATTGATCt                                      >  1:786429/1‑150 (MQ=17)
                                                                                                               gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCACTCATTC‑ATCTTTCT‑GCCCCCCCAAAAGC‑AAAAACCCCCCG‑AA‑G‑C‑GGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTTTCGTGGACAGTCATTAAtatat                                    >  5:815946/1‑150 (MQ=14)
                                                                                                                aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGT‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                                        <  4:1385457/130‑1 (MQ=21)
                                                                                                                aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTGATTG‑ATCTTTCT‑GCCCCGCCAAAAGC‑AAAAAACCCCCG‑AA‑C‑C‑GGGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTGATGTAgg                                   >  8:1231598/1‑151 (MQ=17)
                                                                                                                aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAAATGACAGATAAGCCGGGTTATGTCGTGGACAGTCATTCATCTAgg                                   >  7:556772/1‑151 (MQ=255)
                                                                                                                aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCAATC‑ATAATTGT‑GCCCCGCAAAAAAC‑AAAAACACGCCC‑CA‑C‑C‑GGGTTTTTAACTAAAAAACGTGAAACAGCCCCAAAACCCGGGTTCTTTAGGGGACAGTGATTCATTTa                                     >  7:994465/1‑149 (MQ=9)
                                                                                                                 tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTC‑ATATTTCT‑GCCCCAACAAAACC‑AAAAACCCGCCG‑AA‑G‑A‑CGGTTTTTAAGTAAACCAGGTGAAAATGACCGATAAGAAGGGTTATGTGGTGGAAAgactctgctcaaggc                                  >  4:659850/1‑137 (MQ=2)
                                                                                                                  aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCggg                                                               <  5:729734/121‑1 (MQ=14)
                                                                                                                  aaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTGATTC‑ATCTTTCT‑GCCCCGCCAAAAGC‑AAAAAACCGCCG‑CA‑G‑C‑GGGTTTTTACCTAAATCAGGTGAAACTGACCGATAAGCCCGCTTCTGTGGTGGCCAGTCATTGATCTTGGcc                                 >  7:1602719/1‑151 (MQ=14)
                                                                                                                       gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTA‑ATCTTTGT‑GCCCCTACAAAAAC‑AAAAAACCGCCG‑AA‑G‑C‑GGGTTTTTAAGTAAATAAGGTGAAAAAGACCGATAAGCCGGGTTCTGTACTGGACAGTCATTAATATAGGccacca                             >  6:908065/1‑150 (MQ=255)
                                                                                                                       gCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTC‑ATCTTTCT‑GCCCCTCCAAAATC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTGATTCATCTAGGCCAGCa                             >  2:117224/1‑150 (MQ=255)
                                                                                                                        atttctttTGGGTCTAGAGTCTTTGATAGTCTTTA‑ATCTTTCT‑TCACTTCCTAAAGG‑AATATCTTGCTT‑AA‑G‑C‑TGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                            <  6:1078019/149‑1 (MQ=16)
                                                                                                                         tttctttTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTACAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTTCCCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAATc                          >  5:1431869/1‑151 (MQ=255)
                                                                                                                          ttctttTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAAACCGCCG‑AA‑G‑C‑CGGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCACCAATCg                         >  8:139732/1‑151 (MQ=255)
                                                                                                                           tctttTGGGTATAGCGTCGTGGCCAGTCATTC‑ATCTTTGT‑GCCCCGCCAAAAGC‑AAAAACCCGCCG‑CA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGCTAAGCCGGGTTCTGTCGTGGGCAGTCATTGATCTAGGCCACCAATCGc                        >  3:528542/1‑151 (MQ=255)
                                                                                                                           tctttTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCaa                                                                                                                                  <  3:1128511/50‑1 (MQ=35)
                                                                                                                            ctttTGGGTATAGCGTCGTGGTCAGTCAATC‑AACTTTGT‑GCCCCGCCAAAATC‑AAAAAACCGCCG‑AA‑C‑C‑CGGTTTTTTGCTAAAAAAGGTTAAACAGAACGAAAATCCGGGTTCTTTCGTGGCAAATCATTTATAATTGCCAGCAATAGCt                       >  2:646371/1‑151 (MQ=255)
                                                                                                                             ttttGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCGCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGGTTTTACGTAAATCAGGTGAAACTGCCCGATAAGCCGGGTTCTGTCGTGGGCAGGCAGTCATCTAGGCCAGCAATCGCTc                      >  4:12023/1‑151 (MQ=18)
                                                                                                                              tttGGGTATAGTGTCGTTTACATTCATTC‑ATCTTTCT‑TCCCCTCCAAAAGC‑AAAAACAGGCCG‑AA‑G‑C‑GTGTTTTTACGTAAATCAGTTGAAACTTACTTATAATCCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc                      <  6:1284191/150‑1 (MQ=18)
                                                                                                                               ttGGGTATAGCGTCGTGGACAGTAATTC‑ATCTTTCT‑GCCCCTACAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCTCTCa                     >  2:1222020/1‑150 (MQ=255)
                                                                                                                                tGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTACAAAAGC‑AAAAACCCCCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGCTAAGCCGGGTTCTGTCGTGGCCCGTCATTCATGTAGGCCAGCAATCGCTCACt                   >  4:862946/1‑151 (MQ=18)
                                                                                                                                gcggTACAGCGGCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGCTAAGCCGTTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACt                   <  7:1032348/149‑1 (MQ=18)
                                                                                                                                 gggTATACGGGCGTCGACAGTCAATC‑ATCTTTCT‑GCTCCTCCAAAAGC‑AACAATCCGCCG‑AC‑G‑C‑CGGTTTTTACTTAAATCACGTGTACCGGACCTATAAGCGGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTg                  <  5:1219514/151‑1 (MQ=21)
                                                                                                                                  ggTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg                 >  8:691399/1‑151 (MQ=255)
                                                                                                                                       aGCGTCGTGGACAGTCATTC‑ATATTTCT‑GCCGCACCAAAAGC‑AAAAAACCCCCG‑AA‑G‑C‑GGGTTTTTACGGAAAACACGTGAAACTGGCCGAGAAGCCCGGGTCTGTCGTGGGCACGGAATCATATAGGCCAGCACTCGCTCACAGGCGCa             >  6:316956/1‑150 (MQ=255)
                                                                                                                                          ggcggcgACAGGCATTC‑ATCTTTCT‑GCCCGTCCCAAGGC‑TAATCCCGGCGC‑AA‑G‑G‑GTGTTTTTTCTTATCTGAGGTGAACGTGACGGTTAAGCGGTTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca         <  5:1121553/145‑1 (MQ=255)
                                                                                                                                            cgtGGACAGTCATTC‑ATCTTTTT‑TACTCTCAACAAGC‑AAAACGCCTGCT‑AA‑G‑C‑TTTTTTTTACTTAATTCAGGTTAAAGTGTCTGTTAAGATGTTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcag        <  1:171524/150‑1 (MQ=255)
                                                                                                                                            cgtGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcagc       <  5:225585/151‑1 (MQ=255)
                                                                                                                                             gtGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc      >  6:482130/1‑151 (MQ=255)
                                                                                                                                             gtGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc      >  1:347930/1‑151 (MQ=255)
                                                                                                                                             gtGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc      >  7:197812/1‑151 (MQ=255)
                                                                                                                                               gtaCAGTCATTC‑ATCTTTCT‑GCACATTAAATACC‑AAAATCTCTCCG‑AA‑G‑A‑GGTTTTTTTTGTAATTAAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt     <  2:914437/148‑1 (MQ=25)
                                                                                                                                                 aCAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                                        <  5:574635/97‑1 (MQ=255)
                                                                                                                                                 aCAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc  >  6:1011340/1‑151 (MQ=255)
                                                                                                                                                 aCAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGA‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGTGGAAAGTCTATTATCAAGGCCATCAAGAGGTCACTGCCACAaacacaaaaac  >  6:1289694/1‑144 (MQ=2)
                                                                                                                                                 |                                                                                                                                                             
TAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCT‑GCCCCTCCAAAAGC‑AAAAACCCGCCG‑AA‑G‑C‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACC  >  NC_000913/3270006‑3270301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑