Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1670CG29.1% 229.9 / 56.4 205intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base C (74/50);  new base G (51/0);  total (150/55)
Fisher's exact test for biased strand distribution p-value = 1.32e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGG  >  NC_000913/3270034‑3270316
                                                                                                                                     |                                                                                                                                                         
gcggcatccggctggtCTTTCGCCCACTCCAAAAGCACAAACCCGCCGGAGCGGGTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                                                                                                                          <  8:625746/136‑1 (MQ=11)
   gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGCTAAGCCGCTTTCTTTTGGGTATAGCGTCGGGGCAAGGCAATCATGTTTGCGCCCCCGCCACAGCaaaa                                                                                                                                        >  2:635597/1‑150 (MQ=11)
      aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTAATATTTGTGCGCCCCCAAAAGCAAAAAacc                                                                                                                                    >  7:82606/1‑151 (MQ=11)
             catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGGCATTCATAGTTGCTCGCCGCCAATACCAAAAACccggaga                                                                                                                               >  7:758284/1‑145 (MQ=11)
               tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCTGGGAAAGTCCATCATGGTTAGTCCCCACCAAAACCAAAAACCCGCCG‑CA‑G‑Cg                                                                                                                        >  3:916367/1‑151 (MQ=11)
                  ttCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTCTATAGACTAGTATAAAGTCAATCATAATGTAACACCAGCAAAAGCAAAAACccgcagcagagcgt                                                                                                                        >  1:327360/1‑141 (MQ=2)
                      tatcatCCAAACCCAAAATACAGCAGAAGCTTGTTTTTACGTATATCAGTTGAATATGATCTATATTTCTCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGtt                                                                                                                    <  1:410984/143‑1 (MQ=2)
                       ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGCCAGTCATTCATCGTTGTGCGCGTGCAAAAGCAAAAACCCGGCT‑CA‑G‑Cgggggtttt                                                                                                                >  8:527456/1‑145 (MQ=11)
                           tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTTCGt                                                                                                            >  2:59541/1‑150 (MQ=25)
                                  gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTGCTTCATGGGTGGGCGCCGGCAAAAGCAAAAACCCGGCG‑CA‑G‑GGGGGTT‑TTACGGAAATGAgg                                                                                                    >  5:1056275/1‑151 (MQ=11)
                                   cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGTTTCTGCCCCGCCAAAACCAAAAACCCGCCG‑CA‑G‑GGGGGGT‑TTACGTAAATCAggg                                                                                                   >  7:1529529/1‑150 (MQ=11)
                                    aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCCTTCATGGTTTGGCCCCGGCAAAAGCAAAAACCCGCCG‑CA‑G‑CGCGGTT‑TTTCGGTAATCacggg                                                                                                  >  8:337652/1‑147 (MQ=11)
                                    aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGGTTGTGCCGCTGCAAAAGCAAAAACCCGCCG‑CA‑G‑CGGGGGG‑TTTCGGAAATCAGGTg                                                                                                  >  5:1255856/1‑151 (MQ=11)
                                     attccgatccgcaccGGTTTATTACGTACACCAGGTTAAACTGACTTATAATTATCTTTATTTTGTGTATAGAGTCGTTGACATTCATTTATCTTTTTTCCCATCAATAAGCTAAAACGATCCG‑TA‑T‑CGGGTTT‑TTACGTAAATCAGGTGa                                                                                                 <  4:80062/137‑1 (MQ=255)
                                     aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCGTTGGGCCCCTGCAAAAACAAAAACCCCCCG‑AA‑G‑AGGGTGT‑TTAAGTAAATCaagggg                                                                                                 >  6:474995/1‑146 (MQ=11)
                                        aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTATAGGGTCGTGGCGAGGCATGCATATTTTGGCGCGCGCAAAACCAAAAACCCGCCG‑CA‑G‑AGCGGGG‑TGACGGAAATCAgggccta                                                                                               >  3:954008/1‑145 (MQ=2)
                                           cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGGTTGTGCCCCGCCAAAAGCAAAAACCCGCCG‑AA‑G‑CCGGGTT‑TTAAGTAAATCAGGTGCAACTgc                                                                                           >  5:1331519/1‑150 (MQ=11)
                                           cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAATTTATGCCCCGCCAAAAGCAAAAACCCGCCG‑AA‑G‑AGCGGTT‑TTACCTAAATCAGGTGAAAcagc                                                                                           >  8:764766/1‑148 (MQ=11)
                                             ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTAATGGTTGGGCCCCGGCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGGAAATCAGGTGAAACTGAcc                                                                                         >  7:664865/1‑151 (MQ=11)
                                              cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGCAAGTCAATCAAAGTGAAGCACCAACAAAAACAAAAACCCGCCG‑AA‑G‑ACCGTTT‑TTTCCTAAATGACGTGAAAATGAccc                                                                                        >  2:460276/1‑150 (MQ=2)
                                              cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGATAGCGTCGAGGCCAGGCATTCAACGTTGAGCCCCAGCAAACACAAAAACCCGCCG‑CA‑G‑CCGGGGG‑GTAAGGAAATCAGGGGAAACTGcccc                                                                                        >  6:677217/1‑150 (MQ=2)
                                              cgAAGCGGGTTTTTACGTAAACAAGGTGAAACTGACAGATAAGCATCTTTCTGTTGTGTATAGAGTCGTGGCCAGACATTAAACTGTTTGCACCGACAAAAGCAAAAAACCGCCG‑CA‑G‑CCGGGGT‑GTACGGAATTCAAGTTAAACttcccc                                                                                        >  7:791055/1‑146 (MQ=2)
                                               gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCGCCAAAAGCAAAAACCCGCCG‑CA‑G‑CCGGGGT‑TTAAGTAAATGAAGTGAAACTTACCgc                                                                                       >  3:1069725/1‑150 (MQ=11)
                                                 aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTCATGTTTGTGCCCCTGCAAAAGCAAAAACCCGCCG‑AA‑G‑GGGGGTT‑TTTCGTTAATCAGGTGAAACTGACCGAtt                                                                                     >  5:912832/1‑150 (MQ=11)
                                                 aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTGGTGGAAAGTCAATCATGATTTTACACCATCAAAAACAAAAACCCGCGG‑CA‑G‑ACCGTTT‑TTAAGGAAATGAGGGGAAAATTACCCAt                                                                                      >  4:1340307/1‑150 (MQ=2)
                                                   ccgcttcctACCTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTTTTTTATTGTTCTGGACAGTAATTATTTTTTCTGTATCTCCAAAAGTATTTACCTCCAG‑CA‑G‑GGTGTTT‑TTATGTAAATCAGGTGAAACTGACCGATAAg                                                                                   <  2:732341/143‑1 (MQ=2)
                                                    gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTGCATCATCGTTGTTCCCCCCCAAAACCAAAAAACCCCCGCAC‑G‑GGGGTTT‑TTA‑GTAAAACAGGTGCAACAGCCCGAGaaac                                                                                  >  7:373816/1‑149 (MQ=2)
                                                      gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCCTGGCCAGTCAATAATGCTTCACCGCCTTCAAAATCAATAACCCGCAG‑AA‑G‑ACGGGTT‑TTAAGTAAATCACGAGAAAATGCACGATTAGCCg                                                                                >  7:1405135/1‑151 (MQ=2)
                                                       tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGACCATCAAAATTAGGCACCAACAAAACCAAAAAACCGCCG‑AA‑G‑GGCGTTTGTAACGT‑AATCAACTGAAAATGACCCATTAGCCgg                                                                               >  4:462386/1‑151 (MQ=2)
                                                         tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGCCAGTCAATCATCATTGAGCACCAACAAAAACAAAAACCCGCAG‑CA‑GACCGGGTT‑TT‑CCTAAATCAAGGGAAAATTCCCCATAATCCGGGt                                                                             >  2:318895/1‑151 (MQ=2)
                                                           tACGTACATCAGGTCAAACTGACCGATAAGCCGCTTTTTTTTTTTTATATCGTCGTGGACAGTCATTAATCTTTATGCCAATACAAAATCAAAAAACGTCGG‑TA‑T‑TGGGTTT‑TTACGTAAATCAGGTTAAACTGACCGATa                                                                                     <  6:146690/141‑1 (MQ=2)
                                                           tACGTAAACCAGGTGAAACTGCCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCATCATGTTTTGGCCCCAACAAAAGCAAAAACCCGCCG‑AA‑G‑AGCGTTT‑GTTAGATAATCAGCTGAAACTTAACGATAAGCCCGGGTc                                                                           >  8:256966/1‑151 (MQ=2)
                                                           tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCG‑CA‑G‑CGGGGTT‑TTACGTAAATCAGGTGCAAATGCCCGATAAGCCGGGttg                                                                           >  5:1108230/1‑150 (MQ=14)
                                                           tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATAGTTTGGCCCCTCCAAAAGCAAAAACCCGCCG‑CA‑G‑CGCGGTT‑TTACCGAAATCAGGGGCAACTGACCGCTAAGCCGGGtt                                                                            >  5:390418/1‑150 (MQ=14)
                                                             cGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCATTCGGCCCCAACAAAAACAAAAACCCGCAG‑AA‑G‑CCGGGTT‑TTACCGAAATGAGGTGAAACTTACCCGTAAGCCGGGTTCTg                                                                         >  6:1593069/1‑151 (MQ=14)
                                                              gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAATAAACATTGAGCGCCAACAAAACCAAAAAACCGCCG‑AACC‑CGCGTTT‑TAACG‑AAATCACCTCAAACAGCCCGATAAACCCGGTTCAGt                                                                        >  3:988393/1‑151 (MQ=9)
                                                                   ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGATTGGGCCCCAACAAAAACAAAAACCCGCAG‑AA‑G‑GCGGGGG‑TTAAGTAAATCACGGGAAACTGCCCCATTAGCCGGGTTCAGTctagg                                                                   >  7:502872/1‑147 (MQ=9)
                                                                   ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTACAAAACCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGGCCGATAATCCGGGTTCTGTCGTgg                                                                   >  5:1451392/1‑151 (MQ=255)
                                                                    cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGTTTGTGCCCCAGCAAAAACAAAAACCCGCCG‑CA‑G‑CGGGGTT‑TTACGGAAATGACGTGAAACTTCCCCATAAGCCGGGTTCTGTCGTggc                                                                  >  3:32942/1‑150 (MQ=14)
                                                                      ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCAAATTTCTGCCCCGACAAAAGCAAAAACCCGCCG‑CA‑G‑AGGGGTG‑TTAAGAAAATCACGTGAAAATGACCCATAAGCCGGGTTCTGtgggcgggc                                                                >  1:954452/1‑143 (MQ=14)
                                                                       gtaaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTaa                                                                                                          <  8:1232883/105‑1 (MQ=17)
                                                                       gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCGAGTCCATCATGTTTGTGCACCAGCAAAAACAAAAACCCGCAG‑CA‑GACCGGTTT‑TTCCGT‑AATCACGGGCTAATGACCGATGAGCCGGGttgttttggggtttc                                                               >  5:491123/1‑138 (MQ=2)
                                                                       gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACAGACCGCTAAGCCGGGTTCTGTCGTgggc                                                                 >  4:915770/1‑147 (MQ=255)
                                                                         gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGTACAGTAATTAATATTTAAAAACATACAAAATCAAAAAAACCCCG‑AA‑A‑ACGATTA‑TTAAGTAAATCAGGTGAAAATTACCCATAAGCCGGGttattgagtggagagg                                                              >  6:843255/1‑136 (MQ=2)
                                                                           aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGCTTT‑TTACCTAAATCAGGTGAAACAGACCCAAAAGCCCGGTTCTGTCGATGACACtcat                                                           >  2:1053366/1‑151 (MQ=14)
                                                                             cTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATATTTGTGCGCCAACAAAAACAAAAACCCGCAG‑AATA‑CGGGTTT‑TAAC‑TAAAACAACTGAAAATTAACCATAATACGGGTTgtgttgcgggtagccagt                                                          >  4:1606863/1‑136 (MQ=0)
                                                                             cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGACC‑AA‑G‑GGGGGTT‑TTACGTAAATCACGTGAAAAAGACAGATAATGCTGGTTCTGTCTTGGATAGtgattt                                                         >  8:132443/1‑150 (MQ=14)
                                                                             cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTAATTAATCTTTTTTACCCTACAAAAGCAAAAAACAGCCG‑CA‑G‑CGGGTTT‑TTAAGTAAATAACGTGAAAAAGACCGATAATCCGGGTTCTTTCGTTGTAAGtcaat                                                          >  3:1129341/1‑148 (MQ=9)
                                                                                ccttcccaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACTTTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCTG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                                                      <  8:1513404/144‑1 (MQ=17)
                                                                                 ccGATAAGCCTCTTTCTTTTGTGTATATTTTCGTTTACATTCATTCATCTTTCTTCACCTACAAAATTAAAAACCCTCTT‑AA‑G‑CTTGTTT‑TTACTTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                                                     <  1:1027316/151‑1 (MQ=12)
                                                                                 ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTTTGCCCCTCCAAAAGAAAAAACACGCCC‑AA‑G‑AGGGTTT‑TTAAGTAAAAAAGGTGAAACTTGCCGCTAAGCCGGGTTCTGTTGTTGatcgggagtgcgcc                                                     >  8:658218/1‑138 (MQ=2)
                                                                                  cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTGATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTAAGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTGATTGATCt                                                     >  1:786429/1‑150 (MQ=17)
                                                                                    aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                                                       <  4:1385457/130‑1 (MQ=21)
                                                                                    aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAgg                                                  >  7:1233737/1‑151 (MQ=255)
                                                                                    aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAgg                                                  >  1:147174/1‑151 (MQ=255)
                                                                                    aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAAATGACAGATAAGCCGGGTTATGTCGTGGACAGTCATTCATCTAgg                                                  >  7:556772/1‑151 (MQ=255)
                                                                                    aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCAATCATAATTGTGCCCCGCAAAAAACAAAAACACGCCC‑CA‑C‑CGGGTTT‑TTAACTAAAAAACGTGAAACAGCCCCAAAACCCGGGTTCTTTAGGGGACAGTGATTCATTTa                                                    >  7:994465/1‑149 (MQ=9)
                                                                                     tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCAACAAAACCAAAAACCCGCCG‑AA‑G‑ACGGTTT‑TTAAGTAAACCAGGTGAAAATGACCGATAAGAAGGGTTATGTGGTGGAAAgactctgctcaaggc                                                 >  4:659850/1‑137 (MQ=2)
                                                                                      aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCggg                                                                              <  5:729734/121‑1 (MQ=14)
                                                                                      aaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTGATTCATCTTTCTGCCCCGCCAAAAGCAAAAAACCGCCG‑CA‑G‑CGGGTTT‑TTACCTAAATCAGGTGAAACTGACCGATAAGCCCGCTTCTGTGGTGGCCAGTCATTGATCTTGGcc                                                >  7:1602719/1‑151 (MQ=14)
                                                                                      aaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTCGTGGACAGTCATTCATCTAGGcc                                                >  5:692608/1‑151 (MQ=255)
                                                                                      aaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCAATCATATTTCTGCCCCACCAAAAACAAAAACCCGCCC‑AA‑G‑CCGGTTT‑TTACGTAAATCAGGTTAAACAGACCGATAAGCCCGGTTATGTCGTGGACAGTCAATCATCTacccc                                                >  1:166257/1‑147 (MQ=17)
                                                                                           gCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTAATCTTTGTGCCCCGCCAAAAGCAAAAAACCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTATGTCGTGGCCAGTCATTCATCTAGGCCCGCa                                            >  5:1582851/1‑150 (MQ=17)
                                                                                           gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTGATCTTTCAGCCCCTACAAAAGCAAAAACCCGCCG‑AA‑G‑CCGGGTT‑TTACCTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGACATTCCTCTAGGCCAGCa                                            >  1:65648/1‑150 (MQ=18)
                                                                                           gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGCCCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCa                                            >  5:81377/1‑150 (MQ=255)
                                                                                           gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTGTGCCCCTACAAAAACAAAAAACCGCCG‑AA‑G‑CGGGTTT‑TTAAGTAAATAAGGTGAAAAAGACCGATAAGCCGGGTTCTGTACTGGACAGTCATTAATATAGGccacca                                            >  6:908065/1‑150 (MQ=255)
                                                                                           gCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTGATTCATCTAGGCCAGCa                                            >  2:117224/1‑150 (MQ=255)
                                                                                            atttctttTGGGTCTAGAGTCTTTGATAGTCTTTAATCTTTCTTCACTTCCTAAAGGAATATCTTGCTT‑AA‑G‑CTGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                                           <  6:1078019/149‑1 (MQ=16)
                                                                                              ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCG‑AA‑G‑CCGGTTT‑TTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCACCAATCg                                        >  8:139732/1‑151 (MQ=255)
                                                                                               tctttTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGTGCCCCGCCAAAAGCAAAAACCCGCCG‑CA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGCTAAGCCGGGTTCTGTCGTGGGCAGTCATTGATCTAGGCCACCAATCGc                                       >  3:528542/1‑151 (MQ=255)
                                                                                               tctttTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑CA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCCGTCATTCATCTAGGCCAGCAATCGc                                       >  3:1237418/1‑151 (MQ=255)
                                                                                                ctttTGGGTATAGCGTCGTGGACAGTCATTGAAATTTCTGCCCCTACAAAAACAAAAAAACCCCG‑CA‑A‑CCGGTTT‑TTAAGTAAATAAGGTGAAACTGAACGATAAGCCGGGTTCTGTAGTGGACAGAGATTAATATAGGCCCGCAATAGcg                                      >  6:897417/1‑150 (MQ=255)
                                                                                                ccggcggggATAGCGTCGGGGACTGTCATTCATCTTGCAGCCCCAACAACAGCACCAACCTGCCG‑AC‑G‑GGTGTTT‑TTACGTAATTCAGGCGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCt                                      <  5:169877/146‑1 (MQ=21)
                                                                                                 ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGGTT‑TTACGTAAATCAGGTGAAACTGCCCGATAAGCCGGGTTCTGTCGTGGGCAGGCAGTCATCTAGGCCAGCAATCGCTc                                     >  4:12023/1‑151 (MQ=18)
                                                                                                  tttGGGTATAGTGTCGTTTAGATTTATTCATCTTTCTGCCCCTCCAAAATAAAAAACCCGCCG‑AA‑G‑CGTTTTT‑TTACTTAAATCAGGTGAAACTGATTTATTTTGCGTTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc                                       <  1:1307715/148‑1 (MQ=12)
                                                                                                  tttGGGTATAGTGTCGTTTACATTCATTCATCTTTCTTCCCCTCCAAAAGCAAAAACAGGCCG‑AA‑G‑CGTGTTT‑TTACGTAAATCAGTTGAAACTTACTTATAATCCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc                                     <  6:1284191/150‑1 (MQ=18)
                                                                                                   ttGGGTATAGCGTCGTGGACAGTAATTCATCTTTCTGCCCCTACAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCTCTCa                                    >  2:1222020/1‑150 (MQ=255)
                                                                                                    gcggTACAGCGGCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGCTAAGCCGTTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACt                                  <  7:1032348/149‑1 (MQ=18)
                                                                                                     gggTATACGGGCGTCGACAGTCAATCATCTTTCTGCTCCTCCAAAAGCAACAATCCGCCG‑AC‑G‑CCGGTTT‑TTACTTAAATCACGTGTACCGGACCTATAAGCGGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTg                                 <  5:1219514/151‑1 (MQ=21)
                                                                                                       gTATAGCGTCGTGGACAGTCATTCATATTTGTGCCCCTACAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATAACGTGAAAATGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTAATCTAGGCCAGCAATAGCTCACTGGc                               >  2:1159162/1‑151 (MQ=21)
                                                                                                         ataGCGTCGTTGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CTGGTTT‑TTACTTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc                             <  5:197976/151‑1 (MQ=255)
                                                                                                              ggcggcgACAGGCATTCATCTTTCTGCCCGTCCCAAGGCTAATCCCGGCGC‑AA‑G‑GGTGTTT‑TTTCTTATCTGAGGTGAACGTGACGGTTAAGCGGTTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                        <  5:1121553/145‑1 (MQ=255)
                                                                                                                   gtaCAGTCATTCATCTTTCTGCACATTAAATACCAAAATCTCTCCG‑AA‑G‑AGGTTTT‑TTTTGTAATTAAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                    <  2:914437/148‑1 (MQ=25)
                                                                                                                   ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                    >  8:1230173/1‑150 (MQ=255)
                                                                                                                     aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                                                       <  5:574635/97‑1 (MQ=255)
                                                                                                                     aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc                 >  6:1011340/1‑151 (MQ=255)
                                                                                                                     aCAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGTGGAAAGTCTATTATCAAGGCCATCAAGAGGTCACTGCCACAaacacaaaaac                 >  6:1289694/1‑144 (MQ=2)
                                                                                                                      cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAccc                >  2:210100/1‑151 (MQ=255)
                                                                                                                      cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAccc                >  6:627289/1‑151 (MQ=255)
                                                                                                                       aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg               >  1:119171/1‑151 (MQ=255)
                                                                                                                        gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCTGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg              >  3:122469/1‑151 (MQ=255)
                                                                                                                         tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg             >  8:535562/1‑151 (MQ=255)
                                                                                                                          aattcatCTTTCTGCCCCTCAAAAAGAAAAAACCCGCTG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAATCTGACGTATAAGGCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt            <  1:202921/150‑1 (MQ=255)
                                                                                                                           attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGtt           >  3:62891/1‑151 (MQ=255)
                                                                                                                                tCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGAGAGTGTGTGGTCGAGGCCAGCAATCGTGGACTGGCTCAACCAGCATAacccgcggcag        >  5:767092/1‑141 (MQ=14)
                                                                                                                                 atttCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CTGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTAc     <  6:1552799/150‑1 (MQ=255)
                                                                                                                                  tttCTGCCCCTCCAAAAGTAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGCGTTCTGTGGGGGAGAGTGTGTAGTCTAGGCCTGCAAGGGGTCCCTCGCGCAAGCCCCCTAACCGGCGTCAggccg    >  3:1432785/1‑147 (MQ=2)
                                                                                                                                   ttCAGCCCCTCCAAACGCAACCACCCGCCG‑AA‑G‑CGGTTTT‑TTACGTAAATCAGGTGAAACTGACCGCTAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTa      <  3:766109/148‑1 (MQ=37)
                                                                                                                                    tCTGCCCCTCCAAAAGTAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGGGGAGAGtcagt                                                          >  3:1579799/1‑93 (MQ=14)
                                                                                                                                    tCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACggg  >  6:1375861/1‑151 (MQ=255)
                                                                                                                                     |                                                                                                                                                         
GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AA‑G‑CGGGTTT‑TTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGG  >  NC_000913/3270034‑3270316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑