Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,4570GT26.6% 359.5 / 21.0 243intergenic (+22/‑15)glyV/glyXtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (34/107);  new base T (5/46);  total (43/200)
Fisher's exact test for biased strand distribution p-value = 4.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGC  >  NC_000913/4392309‑4392607
                                                                                                                                                    |                                                                                                                                                        
cccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGca                                                                                                                                                        <  6:1432716/151‑1 (MQ=255)
cccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGc                                                                                                                                                         <  3:834335/150‑1 (MQ=255)
 ccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcac                                                                                                                                                       <  7:661847/151‑1 (MQ=255)
  cGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcaca                                                                                                                                                      <  4:1558793/151‑1 (MQ=255)
   gTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAg                                                                                                                                                     <  2:423229/151‑1 (MQ=255)
     aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGac                                                                                                                                                   <  1:1285460/151‑1 (MQ=255)
     aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGac                                                                                                                                                   <  4:64806/151‑1 (MQ=255)
     aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGa                                                                                                                                                    <  8:1044652/150‑1 (MQ=255)
       cgacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGacca                                                                                                                                                 <  7:502907/151‑1 (MQ=255)
          cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCAcc‑c                                                                                                                                             <  2:61771/151‑1 (MQ=255)
          cgCAGAAAAGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCAcc‑c                                                                                                                                             <  7:993377/151‑1 (MQ=255)
           gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑Ca                                                                                                                                            <  4:459134/151‑1 (MQ=255)
            cAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑Caa                                                                                                                                           <  4:710183/151‑1 (MQ=255)
             aGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGAT‑TAAAGcacaagacaccacaac                                                                                                                                          >  2:226785/1‑139 (MQ=18)
                 aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCCCC‑CAAGCggg                                                                                                                                      >  4:1357706/1‑151 (MQ=255)
                   gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGaa                                                                                                                                    <  5:565907/151‑1 (MQ=255)
                   gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGa                                                                                                                                     <  6:1451522/150‑1 (MQ=255)
                    caaaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAAt                                                                                                                                   <  3:1299178/149‑1 (MQ=255)
                    cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCCCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAAt                                                                                                                                   <  2:831406/151‑1 (MQ=255)
                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATa                                                                                                                                  <  5:1544505/151‑1 (MQ=255)
                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATa                                                                                                                                  <  8:27234/151‑1 (MQ=255)
                            aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAg                                                                                                                            <  8:1482356/150‑1 (MQ=255)
                            aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGt                                                                                                                           <  6:708601/151‑1 (MQ=255)
                            aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGt                                                                                                                           <  5:1621082/151‑1 (MQ=255)
                            aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGt                                                                                                                           <  6:538852/151‑1 (MQ=255)
                              gAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTg                                                                                                                         <  2:1618287/151‑1 (MQ=255)
                               aaaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTgg                                                                                                                        <  8:129942/151‑1 (MQ=255)
                               aaaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTgg                                                                                                                        <  6:420150/151‑1 (MQ=255)
                                aaGCAAAATTAAGTAGTACGCGGGAATATCTAAGTTGGTAGAGGACTACCTTGCGAAGGTCTGGGTCGCTATTTCGAGTCTCGTTTACAGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑GAAGCGGGAATAGCTCAGTTGGt                                                                                                                       <  8:226521/151‑1 (MQ=17)
                                aaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGt                                                                                                                       <  7:1591937/151‑1 (MQ=255)
                                    aaaaTTAAGTAGTACGCGGGAATAGCTAAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTagag                                                                                                                   <  7:861537/151‑1 (MQ=255)
                                    aaaaTTAAGTAGTACGCGGGAATAGCTAAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTagag                                                                                                                   <  8:580558/151‑1 (MQ=255)
                                     aaaTTATGTATTACTAGGGAATATCTCAGTTTGTAGTGGACGACCTTCCCAAGGGCGGGGTCGCTAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCACAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGc                                                                                                                  <  1:511878/151‑1 (MQ=14)
                                     aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGCGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGc                                                                                                                  <  7:1265299/151‑1 (MQ=255)
                                     aaaTTAAGTAGTACGCGGGAATACCTCAGTTGGTAGAGCACGACCTTGCCTAGGTCGGGGTCGCGATTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAATAAAAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGc                                                                                                                  <  2:1133237/151‑1 (MQ=17)
                                       aTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTCCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                                                                                                <  8:183743/151‑1 (MQ=255)
                                          aaGTTGTAGTCGGGAATAGCTCAGTGGGGAGAGCACGTCCTTGCCAACGGAGGGGTCGCGAGTTCGAGCCTCGGTTCCCTCTCATAAATTCGAAAAGTCCGCCAAACAACATAACACC‑TAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc                                                                                                             <  8:1396775/151‑1 (MQ=9)
                                          aaGTAGTACGCGGGAATAGCTCAGTTGGTAGCGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTAGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGa                                                                                                              <  7:2996/150‑1 (MQ=255)
                                            gtcctaCTCGGGAATATCTCAGTTGGTAGAGCTCGAGCTTCCCAACGTAGTGGTCGCGACTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTCCAAACCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                                                                           <  2:748444/147‑1 (MQ=14)
                                            gtagtaCTCGGGAATAGCTCAGTTTGTAGAGCACGACCTTGCCAAGGTCTGGGTCGCTAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                                                                           <  3:124280/151‑1 (MQ=255)
                                             aactaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGTGGTCGCGAGTTCGAGGCGCGTTTCCCGCTCCAAAATTTGAAAAGTCCGTCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                                                                                                          <  1:211230/148‑1 (MQ=14)
                                              actaatcgGGAATACCTCAGTTGTTAGCGGTCGACCAGCCCTAGCGAGGGGTCGCCATTTGGCGGCTCGTTTACGGCTCATAAATTTGAAAAGTTCGTCATACAAAAGAACTCG‑CACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                         <  2:1001910/145‑1 (MQ=2)
                                              actaagctggAATAGATCAGTTTTTAGAGGACGACCTTTCCAATTTATGTGTCGCTATTTCGTGTCTCGTTTACCCCTCCAAAATTTGAAAAGTTCTTCAAATCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                                                                                                          <  1:811362/142‑1 (MQ=2)
                                               gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAATAAAGCAAAACCCACCACAAC‑CAAAAGAGAATAGCTAATATTGTAGATCACGAGCttcc                                                                                                        >  2:277419/1‑149 (MQ=2)
                                                 ccgcgGGAATAGCTCAGTTTTTAGAGCTCGAGCTTCGGTAGGTAGTGGTCGCGATTTCGCGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTCCTTAAAACAACAGACCTAC‑GACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                      <  3:190487/150‑1 (MQ=2)
                                                  gtcgGGAATTGCTCAGTTGGGAGATCACGACCTTTCCAATCGCGTGGTCGCTATATCGACGCTCGTTTCCCTCTCCAAAATTTGAAAAGTCCTCCAAATCAAATACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                      <  3:1033017/148‑1 (MQ=9)
                                                   ccgGGAATAGCTCAGTTGGTAGCGCACGTCCTTGCCCAGGTCGTGGTCGCGAGTTCGCGGCTCGGTTCCCGCGCCAAAATTTGAAAAGTGCTCCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                    <  5:654635/150‑1 (MQ=14)
                                                     gggAATACCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                                                                                                  <  3:580941/151‑1 (MQ=38)
                                                      ggAATATCTCAGTTTGTAGAGCACGTCCTTGCCAAGGGCGGTGTCGCGACTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAACAACATACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                                 <  1:258347/151‑1 (MQ=14)
                                                      ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                                 <  6:927418/151‑1 (MQ=38)
                                                      ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                                 <  7:1353891/151‑1 (MQ=38)
                                                       gAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGAGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                >  5:353156/1‑151 (MQ=255)
                                                            ccTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                           <  8:1291343/150‑1 (MQ=38)
                                                              tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAACCGGGAATAGCTCAGTTGGTAGAGCAAGACCGTGCCAAGGTCGGAGTcgg                                                                                         >  1:109803/1‑150 (MQ=255)
                                                                   tGGTAGCGGACGACCTTGCCAAGGTCGGGGTCGCGAGATCCAGCCGCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCACACCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                     <  3:594594/150‑1 (MQ=14)
                                                                   tGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCGCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                     <  5:4422/150‑1 (MQ=255)
                                                                   tGGTAGAGCACGACCTTGCCAAGGGCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAACCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                     <  5:255579/150‑1 (MQ=255)
                                                                    tgTAGTGCACGAGCTTTCCAAGTGAGGTGTCGCTATTTCGAGTCTCGTTTCCGTCTCCAAAATTTGAAAAGTGCTTCACATCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                    <  1:908723/149‑1 (MQ=14)
                                                                     ttAGAGCACGTCCTTCCGAATGTCGTGGTCGCGAGTTCGAGTCTCGTTTACCTCTCCAAAATTTGAAAAGTGCTGCAAATCACATACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                   <  2:1430927/149‑1 (MQ=14)
                                                                     gtagcgctggACCTTTCCAAGGTCTGGGTCGCTAGTTCGAGTCTCGTTTACCGCTCCAAAATTTGAAAATTGCTGCAAATCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                   <  2:1219685/141‑1 (MQ=14)
                                                                     gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAATCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCg                                                                                  <  6:1166463/151‑1 (MQ=38)
                                                                           caggagcTTGCCAAGGTCGGTGTCGCTAGTTCGAGTCTCGTTTCCCGCTCCTAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                            <  6:1332584/145‑1 (MQ=14)
                                                                           cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                            <  8:1197509/151‑1 (MQ=255)
                                                                               aCCTTGCGAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                                        <  6:174471/151‑1 (MQ=255)
                                                                                ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAACGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                       <  7:953338/151‑1 (MQ=25)
                                                                                   tggcgagtgcgtggTCGCGAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTCCTTCAAATCACATACCTCC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                      <  4:27065/137‑1 (MQ=14)
                                                                                    gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAATCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                    <  7:1025405/150‑1 (MQ=255)
                                                                                     cctagttctgggTCGCTATTTCGTGCCTCGTTTCCGTCTCAAAAATTTGAAAAGTCCTTCATACAACAGACGAAC‑CACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑t                                                                 <  2:1407325/142‑1 (MQ=14)
                                                                                      caagtgaggggTCGCGAGTTCGAGGCGCGTTTCCCGCTCAAAAATTTGAAAAGTCCGGCAAATCACAGACCAAC‑GAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑Tc                                                                <  3:731143/144‑1 (MQ=14)
                                                                                       aaggtctttgTCGCGACATCGACTCTCCTTTACCACTCCAAAATTCGAAAATTCCTAAACAAAACATACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑Tcc                                                               <  5:1168931/142‑1 (MQ=11)
                                                                                       aaGGGCGGGGTCGCTAGATCGCGGCTCGGTTCCCGCTCCCAAATTTGAAACGTGCTGCACCCCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑Tcc                                                               <  8:1536632/151‑1 (MQ=11)
                                                                                        atgtatgtggagcgAGTTCGAGTCTCGTTTCCGTCTCATAAATGTGAAAATTTCTTCAAATCACAGACGTAC‑GAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑Tcc                                                               <  6:1010249/139‑1 (MQ=14)
                                                                                        agcgcGGTGTCGCGAGTTCGAGGCACCTTTCCCGCTCCAAAATTTGAAAAGTGCTGCACAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCa                                                              <  3:22848/147‑1 (MQ=14)
                                                                                            cGGTGTCGCTAGTTCGACTCTCGTTTCCCTCTCATAAATTTGAAAAGTCCTTCATATAAAAGACGACC‑TAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCaaaa                                                           <  6:757696/150‑1 (MQ=14)
                                                                                            agtggtcgcttcatCGAGCCTCGTTTCCCGCTCATAAATTTGAAAAGTTCTTAATATAACAGACCTCG‑GAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCaaaa                                                           <  4:785229/137‑1 (MQ=11)
                                                                                                  cgcgAGATCGAGTCTCGTTTCCCTCTCAAAAATTAGAAAATTCCTCAAAACCACATACCACC‑AAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGaaa                                                    <  5:1345052/151‑1 (MQ=14)
                                                                                                      atttCGAGGCTCGTTTCCCGCTCCTAAATTTGAAAAGTGCTTAAAATCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTg                                                 <  4:84575/148‑1 (MQ=14)
                                                                                                          ggcgtgtcGTTTCCCGCTCATAAATTTGAAAAGTGCGTCACATAACAGACGTCG‑TAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGt                                             <  2:1344374/144‑1 (MQ=17)
                                                                                                           gtgtgtcGTTACCCTCTCAAAAATTTGAAAAGTTCTCAATAAAACAGAACAAG‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTaa                                           <  8:246673/146‑1 (MQ=14)
                                                                                                                 cctttacgccTCAAAAATTGGAAAATTCCTGCACACCACATACCACG‑CACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGcaca                                     <  5:602072/142‑1 (MQ=14)
                                                                                                                 cGTTTCCGTCTCATAAATTTGAAAATTTCTTAATATAACATACCTCC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGcaca                                     <  1:1534882/151‑1 (MQ=14)
                                                                                                                   tttCCCGCTCCAAAATTTGAAAAGTGCTGAAAAGCACCCCCCACC‑CCAGCGGGAATAGCGCAGTTGGTAGCGCACGACGGAGCGCAGGTCGCGGTCGCGCGTGGGCGTGTCGGTTGCCGCGGGCAAAATTT‑AAAGGGCTGTAAggggccgg                                   >  7:1426163/1‑145 (MQ=9)
                                                                                                                    tttcctatgcAAAATTACAACAGTGCTCCAAAGCACAGAGCGCCACAGGCGTTAAT‑CCTCCGGCGCTACCGAACTACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGa                                   <  5:1318320/141‑1 (MQ=9)
                                                                                                                     tCCCGCTCCTAAATTGGAAAAGTCCTAGATATCAAAGACGACG‑GAAGCTGTAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTTAAAGTGCTGTAAGGCACAGac                                  <  3:1364989/150‑1 (MQ=17)
                                                                                                                     accccCTCCAAAATTTGAAAAGTCCTCCACACAACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGacc                                 <  5:773581/150‑1 (MQ=18)
                                                                                                                      ccgccTCCAAAATTTGAAAAGTACTGCACAGAACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGacca                                <  5:865027/147‑1 (MQ=255)
                                                                                                                         ccTCAAAAATTTGAAAAGTGCTCCAAAACACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCAccc                             <  7:244294/150‑1 (MQ=255)
                                                                                                                           tCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGCAATTGCTGTAAGGCACAGACCCCCCac                           >  5:1125943/1‑150 (MQ=255)
                                                                                                                           gcaaaaaTTTGAAAAGTCCTACAAAGAACAGACCTCG‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCaa                           <  5:1244708/148‑1 (MQ=17)
                                                                                                                            ccAAAATTTGATAAGTCCTCCAACGCACTGACCTCG‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCACGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCaa                           <  6:1251049/150‑1 (MQ=17)
                                                                                                                              aaaaTTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGCAAGGCCTGTAAGCGACAGAGCCCCCAACCg                        >  5:1405273/1‑151 (MQ=17)
                                                                                                                               aaaTTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGATTCTCGTTTCCCGC‑TCCAAAATTTGAAATGGATGAAATTCACACACCACCCAAACgg                       >  4:999917/1‑151 (MQ=17)
                                                                                                                                   ttGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTTAAAGTGCTGAAATGAACAGACCACCCACGCGGGatt                   >  8:366941/1‑149 (MQ=17)
                                                                                                                                   tcgAAAAGTTCTCAAAAAAACATAACACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGaa                    <  4:1165704/148‑1 (MQ=14)
                                                                                                                                    tGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAATGGACAGACCACCCAAGCGGGAAt                   >  6:373673/1‑150 (MQ=255)
                                                                                                                                     gAAAAGTGCTTAAAAACAAAGACCACC‑AAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATa                  <  8:510471/150‑1 (MQ=255)
                                                                                                                                      aaaaTTCCTCCAAATAACATACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCTAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGc                <  4:1071518/151‑1 (MQ=14)
                                                                                                                                                cAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTGCCGC‑TCCAAAATTTTAAAGTGCTGTAATGCACACACCCCCCAACCGGGCATAGCTCAGTTGGtc      >  7:85138/1‑150 (MQ=17)
                                                                                                                                                 aaaGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGAACAGACCACCCAAGCGGGAATTGCTCAGTTGGTat     >  8:1584522/1‑150 (MQ=255)
                                                                                                                                                   aGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGCAAGTGCTGTAATGGCCAGACCCCCCCCGGGGGAATatgggcgttgttag     >  7:1185376/1‑136 (MQ=14)
                                                                                                                                                    |                                                                                                                                                        
CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACC‑CAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGC‑TCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGC  >  NC_000913/4392309‑4392607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑