Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,188,572 C→A R1075S (CGT→AGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,188,5720CA99.8% 1618.4 / NA 426R1075S (CGT→AGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (201/224);  total (201/225)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

GCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGT  >  NC_000913/4188424‑4188718
                                                                                                                                                    |                                                                                                                                                  
gCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGt                                                                                                                                                  <  1:255247/151‑1 (MQ=255)
gCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGt                                                                                                                                                  <  8:285698/151‑1 (MQ=255)
 ccGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTc                                                                                                                                                 >  5:551949/1‑151 (MQ=255)
   ggTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTCAGTCCg                                                                                                                                               >  6:1446757/1‑151 (MQ=255)
      tATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGc                                                                                                                                             <  7:664924/150‑1 (MQ=255)
       aTCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCAc                                                                                                                                           >  8:324041/1‑151 (MQ=255)
         cACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTg                                                                                                                                         <  7:1621724/151‑1 (MQ=255)
            cGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGaa                                                                                                                                       >  3:1085811/1‑150 (MQ=255)
             gAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGaaa                                                                                                                                      >  4:1140509/1‑150 (MQ=255)
              aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAAt                                                                                                                                    >  5:238517/1‑151 (MQ=255)
               aGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATc                                                                                                                                   <  4:451925/151‑1 (MQ=255)
                     cGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGAt                                                                                                                             <  3:1585501/151‑1 (MQ=255)
                     cGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGAt                                                                                                                             <  3:847013/151‑1 (MQ=255)
                     cGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGAt                                                                                                                             <  4:977647/151‑1 (MQ=255)
                      ggTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGGCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATg                                                                                                                            <  6:1295626/151‑1 (MQ=255)
                         tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                          <  4:1609755/150‑1 (MQ=255)
                          ttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTc                                                                                                                         <  5:760002/150‑1 (MQ=255)
                            gTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAgg                                                                                                                      <  6:167216/151‑1 (MQ=255)
                            gTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAgg                                                                                                                      >  7:879016/1‑151 (MQ=255)
                              aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGt                                                                                                                    >  4:1032344/1‑151 (MQ=255)
                              aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGt                                                                                                                    >  1:1623612/1‑151 (MQ=255)
                                   ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAac                                                                                                                 <  8:1025022/149‑1 (MQ=255)
                                     aCTGACATGATCTACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGTTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgac                                                                                                              <  1:1106281/150‑1 (MQ=255)
                                        gACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGt                                                                                                            <  2:2189/149‑1 (MQ=255)
                                        gACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCACAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                          >  1:1325135/1‑151 (MQ=255)
                                         aCATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCt                                                                                                         >  7:551498/1‑151 (MQ=255)
                                          cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                                        >  2:484543/1‑151 (MQ=255)
                                               tCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                                                                                   >  2:274291/1‑151 (MQ=255)
                                                cGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                                                                                   >  4:756957/1‑150 (MQ=255)
                                                cGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCa                                                                                                  <  8:38518/151‑1 (MQ=255)
                                                 gACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAg                                                                                                 <  7:1439072/151‑1 (MQ=255)
                                                      ccAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAcc                                                                                            >  1:728123/1‑151 (MQ=255)
                                                      ccAGACCATTACGCGTCAGACCGACGAACTGACCGGGCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAcc                                                                                            <  1:484732/151‑1 (MQ=255)
                                                        aGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGCCGTTCTGATCCCAGGTACCg                                                                                           >  6:327988/1‑150 (MQ=255)
                                                         gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGat                                                                                         >  2:60363/1‑151 (MQ=255)
                                                           ccATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                                       <  1:1238454/151‑1 (MQ=255)
                                                            cATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATg                                                                                      <  4:88449/151‑1 (MQ=255)
                                                             aTTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGATCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACAGAAAATCGTTGATGCTCAGGGTAACGACGTTATGATCCCAGGTAACGATATGc                                                                                     >  2:1394699/1‑151 (MQ=255)
                                                               tACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGcc                                                                                    >  3:1231121/1‑150 (MQ=255)
                                                                aCGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGTTGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGca                                                                                    >  6:417399/1‑148 (MQ=255)
                                                                 cgcgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgc                                                                                 <  7:1031354/151‑1 (MQ=255)
                                                                 cgcgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgc                                                                                 <  3:791806/151‑1 (MQ=255)
                                                                 cgcgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgc                                                                                 <  7:3473/151‑1 (MQ=255)
                                                                 cgcgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTCGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCCgc                                                                                 >  4:318451/1‑151 (MQ=255)
                                                                  gcgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                                <  5:1332553/151‑1 (MQ=255)
                                                                    gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                              <  8:1120081/151‑1 (MQ=255)
                                                                     tCAGACCGACGAACTGACCGGTCTGTCTGGGGTGTTGGTTGTGGATGCCGCAGAACTTACCGCAGGTGGTAAAGATCTGAGTCGGGACAGGAAATTCTTTGTTGAAAAGGGTACCGAGGTTCGGAGCCCCGGTACCGATATGCCTgcgc                                                                               >  6:790378/1‑149 (MQ=255)
                                                                      cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGt                                                                            >  6:1094423/1‑151 (MQ=255)
                                                                       aGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTa                                                                           <  5:383145/151‑1 (MQ=255)
                                                                             acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCt                                                                     <  3:217517/151‑1 (MQ=255)
                                                                               gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATACCAGGTACCGATATGCCGGCGCAGTACTTCCTGc                                                                   >  4:58623/1‑151 (MQ=255)
                                                                                aaCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGcc                                                                  >  3:1185775/1‑151 (MQ=255)
                                                                                aaCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGc                                                                   <  2:1239132/150‑1 (MQ=255)
                                                                                 aCTGACGTGTGTGTTTTCGCTGGTGGTTGTGGATTCGGGAGAAGGTACCGCAGGTGGTATAGATCTGAGTCCGGCACTGAATATCGTTGATGCTCAGGGTAACGAGGTTCTGATCCCAGGTACCGATATGCCAGCGGAGTACTTCCTGCCg                                                                 <  7:128078/151‑1 (MQ=255)
                                                                                  cTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCgg                                                                >  7:738665/1‑151 (MQ=255)
                                                                                    gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt                                                              <  7:1115754/151‑1 (MQ=255)
                                                                                    gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCACAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt                                                              <  3:911322/151‑1 (MQ=255)
                                                                                     aCCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt                                                              >  3:509665/1‑150 (MQ=255)
                                                                                     aCCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTa                                                             <  5:706160/151‑1 (MQ=255)
                                                                                           ctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAcgcg                                                        >  2:842136/1‑150 (MQ=255)
                                                                                             gtctTCGCTGGAGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCCGCACTGAAAATCGTAGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGAAAAGCGAtt                                                     >  5:1037031/1‑151 (MQ=255)
                                                                                               ctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGt                                                   >  2:1477655/1‑151 (MQ=255)
                                                                                                  ggCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTATCGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAATGCGATTGTGCa                                                <  6:742701/150‑1 (MQ=255)
                                                                                                   gCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                               <  2:377416/151‑1 (MQ=255)
                                                                                                     tggtggTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCt                                             >  2:731908/1‑151 (MQ=255)
                                                                                                      ggtggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTg                                            >  2:1335167/1‑151 (MQ=255)
                                                                                                      ggtggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTg                                            >  8:602375/1‑151 (MQ=255)
                                                                                                       gtggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTgg                                           <  4:146236/151‑1 (MQ=255)
                                                                                                       gtggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTgg                                           <  6:1510148/151‑1 (MQ=255)
                                                                                                         ggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                          >  6:1319892/1‑150 (MQ=255)
                                                                                                         ggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATACCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGaa                                         <  8:1355901/151‑1 (MQ=255)
                                                                                                            tCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                                       <  7:1388993/150‑1 (MQ=255)
                                                                                                              tGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATgg                                    >  6:1193015/1‑151 (MQ=255)
                                                                                                               ggATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGAt                                      <  3:1468212/148‑1 (MQ=255)
                                                                                                               ggATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATgg                                    <  8:858283/150‑1 (MQ=255)
                                                                                                               ggATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                                   >  3:414763/1‑151 (MQ=255)
                                                                                                                  ttCCGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGt                                 >  7:1544203/1‑150 (MQ=255)
                                                                                                                    ccGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTAc                               <  1:648754/150‑1 (MQ=255)
                                                                                                                    ccGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTAc                               <  6:525134/150‑1 (MQ=255)
                                                                                                                    ccGCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTAc                               <  4:1293125/150‑1 (MQ=255)
                                                                                                                      gCAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTAc                               >  5:1608069/1‑148 (MQ=255)
                                                                                                                      gCAGAACGTACCGCAGGTGGTAAAGAGCTGAGTCCGGCACTGAAAATCGTGGATGCTCCGGGTAACGACGTGCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGa                            <  6:598373/151‑1 (MQ=255)
                                                                                                                       cAGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                           <  2:954034/151‑1 (MQ=255)
                                                                                                                        aGAACGTACCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTCCAGCTGGAAGATGGCGTACAGATc                          <  8:224244/151‑1 (MQ=255)
                                                                                                                               aCCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTg                   >  5:1469714/1‑151 (MQ=255)
                                                                                                                               aCCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTg                   <  5:1185702/151‑1 (MQ=255)
                                                                                                                               aCCGCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTg                   >  2:1584575/1‑151 (MQ=255)
                                                                                                                                  gCAGGTGGTAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTg                <  2:1057265/151‑1 (MQ=255)
                                                                                                                                    aGGTGGTAAAGATCTGAGTCCGCCACTGAAAATCCTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTCTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGa               >  6:1544739/1‑150 (MQ=255)
                                                                                                                                         gtAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTTGTGACACCCt         <  2:1018851/151‑1 (MQ=255)
                                                                                                                                         gtAAAGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt         <  8:1089258/151‑1 (MQ=255)
                                                                                                                                           aaaGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTgg       >  7:250759/1‑151 (MQ=255)
                                                                                                                                           aaaGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTgg       <  1:310586/151‑1 (MQ=255)
                                                                                                                                           aaaGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTgg       <  1:1563453/151‑1 (MQ=255)
                                                                                                                                           aaaGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTgg       <  1:1004052/151‑1 (MQ=255)
                                                                                                                                            aaGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACATATCAGCTCTGGTGACACCCAGgc      >  8:1544740/1‑151 (MQ=255)
                                                                                                                                            aaGATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGgc      <  5:608526/151‑1 (MQ=255)
                                                                                                                                              gATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGgcgc    >  3:597616/1‑151 (MQ=255)
                                                                                                                                              gATCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGgcgc    >  1:601446/1‑151 (MQ=255)
                                                                                                                                                tCTGAGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGt  >  4:1354668/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGT  >  NC_000913/4188424‑4188718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑