Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG29.8% 523.8 / 92.8 336intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (70/165);  new base G (98/2);  total (168/168)
Fisher's exact test for biased strand distribution p-value = 1.91e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579
                                                                                                                                                     |                                                                                                                                                      
  ttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtccg                                                                                                                                                     >  3:291686/1‑150 (MQ=255)
     gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                                  <  2:86458/151‑1 (MQ=255)
        gggATTGTTGGGCTGTTGATTGGCTGGAGGTGTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccc                                                                                                                                               <  7:45000/150‑1 (MQ=255)
          gATTGGTGTGCTGTAGATTGGCGGGATGGTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                             <  4:891632/151‑1 (MQ=255)
           aTTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGCTGCTCCTccccc                                                                                                                                             >  5:1281333/1‑150 (MQ=255)
                 gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCGGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                      <  8:1450104/151‑1 (MQ=255)
                 gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAatat                                                                                                                                       <  3:791681/150‑1 (MQ=255)
                 gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                      <  3:734500/151‑1 (MQ=255)
                 gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCTCCCCCCAAAATc                                                                                                                                      >  7:125606/1‑151 (MQ=255)
                  tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATAAcc                                                                                                                                     >  8:1218901/1‑151 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCATGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                   <  2:92547/149‑1 (MQ=255)
                         gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGTCTGAAAACCATTAATGCTTGCGGTCTGTAAGCCGGTTCATGATGAAATCCTGATCCTGCTATTCAGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                              <  1:104867/151‑1 (MQ=255)
                            tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                            <  2:1399203/150‑1 (MQ=255)
                             ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                          <  5:122890/151‑1 (MQ=255)
                                tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGGCGGCTCCCCCCAATATCCTGCACTTgaggagg                                                                                                                       >  2:1608432/1‑150 (MQ=255)
                                tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCTCCTCCCCC‑AATAACCTTGAgctgattcggg                                                                                                                      >  3:209225/1‑146 (MQ=255)
                                tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTGCCCCCAAAATCCTTCAGaggatgcgg                                                                                                                       >  3:106842/1‑148 (MQ=255)
                                 ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                        <  5:489454/149‑1 (MQ=255)
                                   aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGGCGCGGCGCCCCACTAGACGTCAGATGATGAGTg                                                                                                                      >  4:759908/1‑149 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGGCGGCCTCCGCCCCCAACTATAGGTGAGATGATGAGTGAACCTg                                                                                                                >  4:1283377/1‑151 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                 <  3:1297971/150‑1 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                >  4:357685/1‑151 (MQ=255)
                                        tAATCGCCGCAACGATCGTCATCTGGCTTTAAACCAGTAATCCTTTCGTTCTGTTAGCCGGTTCAGGATGAAATCCTGATCCGGCTATTCCGAAAGTTATCAGCCACCTTCCTCCTCCCGCAAATATCCTTCAGATCATGAGTGATCCTg                                                                                                                <  7:13926/150‑1 (MQ=255)
                                        tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                <  5:1505745/150‑1 (MQ=255)
                                        tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                <  2:1280458/150‑1 (MQ=255)
                                         aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGTTGAGGAGTGAtgctgcc                                                                                                              >  4:406435/1‑150 (MQ=255)
                                           aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                            >  1:1611528/1‑151 (MQ=255)
                                               cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                        <  6:1030885/151‑1 (MQ=255)
                                                gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGGGCCGCCCCCCAAGATCCTTCAGATGCTGAGTGATCCTGGATTAGGCt                                                                                                       >  5:310087/1‑151 (MQ=255)
                                                 ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                      <  6:863389/151‑1 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCGCCTCCCCCCAATATCCTTCAGATGATGAGTGCTCCTGGATTAGGCTATg                                                                                                    >  5:394171/1‑151 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCGTGCGCGGCTGCGGGCGCCGGCATTAGGCTATg                                                                                                    >  4:330328/1‑151 (MQ=255)
                                                    cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                   <  2:832330/151‑1 (MQ=255)
                                                         gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCGGCATTTTGCGATGGCAAgg                                                                                              >  6:432485/1‑151 (MQ=255)
                                                         gTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                              <  6:545414/151‑1 (MQ=255)
                                                           cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                            <  5:156341/151‑1 (MQ=255)
                                                              ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATCCTTCAGATGATGAGTGAGCCTGCATTAGGCTATGGCAAGGTGATc                                                                                         >  4:685952/1‑151 (MQ=255)
                                                                ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCTCCCCCGACTAGCCGGCAGAGGATGAGGGGTCCTGCATTTGGCTGTGGCTAggggctc                                                                                         >  2:497715/1‑144 (MQ=255)
                                                                    gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGGCGCCCCCACATATCCTGCCCAGGAGGGGTGCTCCTGCAGTGGGGTCTGGGAAGGGGaggagtttt                                                                                   >  6:1080424/1‑143 (MQ=255)
                                                                     aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                  <  2:408145/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGCCGCGGCCCCCCACGATCCTTCAGATGATGAGTGCTGCTGCCTTAGGGTATGGCAGGGTGCTCAGCTTTtgatgcgagg                                                                         >  6:281188/1‑145 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCACATAACCATCAGATGATGAGAGATCCTGAAATAGGGTATGGAAAGGTGATCAGATTTTCATCACAgc                                                                         >  8:900802/1‑150 (MQ=255)
                                                                                accTTGCGTTCTGTAAGCCGGTTCAGGAGGAAAGCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                       <  5:1074602/150‑1 (MQ=255)
                                                                                 ccTTGCGGTCTGTAAGCCGGTTCAGGAGGAACTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                       <  1:1282046/150‑1 (MQ=255)
                                                                                  cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGGCCCCAAATATCGGTCAGATGATGAGTGATCCTGCATGAGGGTATGGCAAGGGGATCAGAGTTTCATGACAggggc                                                                      >  6:400216/1‑149 (MQ=255)
                                                                                   ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAAAATCCTTCAGATGATGAGTGAGCGTGGAGTAGGGGGTGGCAAGGGGGTGTGATTTTGTTCAGAGGGGatt                                                                    >  8:1485851/1‑149 (MQ=255)
                                                                                   ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCGTCGCCCAAAGATACTTCAGATGATGAGTGATGCGGCATTAGGGGATTGCTAGGTGCTCAGATTTTGATAACACGGGAAt                                                                    >  6:954633/1‑151 (MQ=255)
                                                                                    tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                    <  5:790376/150‑1 (MQ=255)
                                                                                    tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCGCCTCCCCCAAATATAATTCAGAAGATGATTGATACTGCATTAGTTAATGGAAAGGTGATGATATTTTAATCAAATGCGAAtt                                                                   >  4:152643/1‑151 (MQ=255)
                                                                                     gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCTGCCCCAAATATCCTTCAGATGATGAGTGATCATGCATTAGGCTATGGGAAGGTGGTCAGATTTTAATCACAGGGGGAttt                                                                  >  4:598912/1‑150 (MQ=255)
                                                                                      cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGGCCCCAAATATACTTCAGATGAGGAGTGATGCTGCGTTAGGAGATTGCACGGGTAGCGGATGTTGATCAAGCGGGAATTat                                                                 >  1:742/1‑151 (MQ=255)
                                                                                      cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCGTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTGATAACAGGGGTAGTat                                                                 >  8:3102/1‑151 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                           <  8:735953/151‑1 (MQ=255)
                                                                                            gTAAGCCGGGTCAGGAGGAAATCCGGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                              <  5:668460/148‑1 (MQ=255)
                                                                                            gTAACCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                           <  8:95704/151‑1 (MQ=255)
                                                                                             tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCGCCCGGCCTCCACACGCCAAGATCCTACAGAAGATGAGTGAACAGGCACTAGGCTATCGCAAGTATCTCAGATATTATTGACAGGGGAATTatcaattt                                                           >  1:923343/1‑144 (MQ=255)
                                                                                              aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCCAAAAACCGTAAGATGATGAGTGATGCTGGGGTAGGCGATGGCAAGGTGATGAGATTTTCTTGACAGGGGAATTTTGATGtttt                                                         >  3:1533382/1‑149 (MQ=255)
                                                                                              aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                          <  6:1506787/150‑1 (MQ=255)
                                                                                               aGCCGGGTCATGAGGTAATCCTTATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATTATGAGTGATCCTGCATTAGGCTATGTCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                        <  3:1355418/151‑1 (MQ=255)
                                                                                                gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGGCTCGGCCCCCCCAGATCCTTCAGATGATGCGGGATCCTGCATTCGGCTAGGGGTCGGTGAGCAGCTTTTGATCAGAGGGGTATTATGCTGTTaagt                                                       >  6:1344913/1‑149 (MQ=255)
                                                                                                 ccGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                      <  7:992321/151‑1 (MQ=255)
                                                                                                  gggTTCAGGAGGAAATGCTTATCCGGCTATTCGGATAGTTATCCGCCGCCGTCCTGCTCCCCCAAATATCGTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                     <  3:916671/150‑1 (MQ=255)
                                                                                                  cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCGGCCGTCCGCCGCCCCCCAATATCCTTAAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATAACAGGGGAAGTATGAGGTTAAATg                                                      >  1:1136662/1‑150 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCATCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                    <  3:394784/151‑1 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATgg                                                 <  1:872842/150‑1 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCGGTCCTCCGCCCCCAAAAATAATACAGATGATGAGTGATGCTGAATTAGGGGAAGGCAAGGTGATCAGCTTTTCATAACAGGCGAAATATAATGTTATATGAAttg                                                 >  7:306857/1‑148 (MQ=255)
                                                                                                        aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGTCCTCCACCCCCAAATAAAAGTCAAATTATTAGTGATACAGCAATAGGCGATGGAAAGGTGAACAGAGATTCAGAACAGGCCAATGATGATGTTAaatgaatgtc                                                >  5:193899/1‑148 (MQ=255)
                                                                                                         ggaggaAATCCTGATCCGGCTATTCCGAACGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAGCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAc                                              <  4:540685/151‑1 (MQ=255)
                                                                                                           aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                            <  8:517026/151‑1 (MQ=255)
                                                                                                            ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                           <  7:1032658/151‑1 (MQ=255)
                                                                                                             gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCTCCCCCAAAAATACATAATATGATGAGTGAACATGCATTAGTCATTGGGATGGTGAGCAGATTTTAAAGAAAGGGGAATTATGATTTTAATTGCATGTCACCTGc                                          >  1:1014212/1‑151 (MQ=255)
                                                                                                               aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCg                                        <  3:302700/151‑1 (MQ=255)
                                                                                                                aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTAAGATGATGAGTGATACTGCATTAGGCTATGGGAAGGTGATCAGATTTTAATAAAAGGGGAATTATTATTTTAAATGAATGGCACCTGCCgg                                       >  2:259556/1‑151 (MQ=255)
                                                                                                                  ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                     <  3:1245269/151‑1 (MQ=255)
                                                                                                                  ccTGATCCGGCTATTCCGAAAGCTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                      <  7:595602/150‑1 (MQ=255)
                                                                                                                   cTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCAAATAACCCTCAGATGATGAGTGATGCTGCAGTTGGCTATGGGAAGGTGCTCAGATTTTCATCACAGGGGAATTATGATGTTAAATGAATTGCACCTGCAGGTgg                                    >  3:1023893/1‑150 (MQ=255)
                                                                                                                    tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATAATTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTTTGATTTTAAATTCATGGCCCCAGCCGGGGcc                                   >  7:682417/1‑151 (MQ=255)
                                                                                                                    tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATACTTAAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTAAATGCATGGCAccggccgggggc                                   >  1:1338397/1‑147 (MQ=255)
                                                                                                                     gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcc                                   <  2:1303966/150‑1 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                               <  5:885765/151‑1 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATCATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                               <  8:1557305/151‑1 (MQ=255)
                                                                                                                         cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                              <  5:1307189/151‑1 (MQ=255)
                                                                                                                          ggCTATTCCGAAAGTTATCCGGGCCCGGCCGCCGCGCCCAAATATACTTAAGATGATGAGTGATGCTGGATTAGTGTATGGGAAGGGGATCAGATTTTAATAACAGGGGAATTATGATTTTAAATGAATGGAAGATGCCGGGGCCCCCAtt                             >  1:574208/1‑151 (MQ=255)
                                                                                                                          ggCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGGGATCCTGGATTAGGCTATGGCAAGGGGATCAGATTTAGATCACAGGGGAATTATGATGTTAAATTAATGGAACCTGCCGGGGgcccc                                >  4:243106/1‑148 (MQ=255)
                                                                                                                             tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTAAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATGATGTTTAATGAATGTCACCTGCCGGTGCCCCCATTTGt                          >  1:1472581/1‑151 (MQ=255)
                                                                                                                             tATTCCGAAAGTTATCCGCCCCCCGCCGCCTCCCCCAAAAAAACATCACATGATGAATTATACAGCATTAGGCTATTGCAACGTGCTCAGAATTTCTTCACAGCGGAATTATAATCTTAAAAGAAAGGCAACTCCCCCGGCCCCCAGTTGt                          >  7:1195536/1‑151 (MQ=255)
                                                                                                                              aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                           <  5:552728/149‑1 (MQ=255)
                                                                                                                              aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                          <  2:94844/150‑1 (MQ=255)
                                                                                                                               atCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                        <  3:64672/150‑1 (MQ=255)
                                                                                                                                tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                        <  5:449576/150‑1 (MQ=255)
                                                                                                                                  cGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAATGCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCAAAGGGGAATTATGATTTTAAATGCATGGCACCTGCCGGGGCCCCCAATTGTTaaa                      >  4:1113882/1‑150 (MQ=255)
                                                                                                                                     aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                  <  2:1471211/151‑1 (MQ=255)
                                                                                                                                      aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                 <  8:67897/151‑1 (MQ=255)
                                                                                                                                          aTCCGCCCCCGGCCTCCTCCCCCAAATAACCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTAATCACAGGGCAATTATTATGTTAAATTCATGGCACCTGCCGGTGCCCCCATTTGTTTAAcaaagaaa              >  7:768540/1‑150 (MQ=255)
                                                                                                                                            ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag            <  3:587225/150‑1 (MQ=255)
                                                                                                                                                 cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      >  6:759753/1‑151 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  4:1056221/151‑1 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  8:202232/151‑1 (MQ=255)
                                                                                                                                                    gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   >  4:1378146/1‑151 (MQ=255)
                                                                                                                                                    gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   >  7:1600675/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑