Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4320TG25.8% 643.5 / 67.4 336intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (81/166);  new base G (86/0);  total (168/168)
Fisher's exact test for biased strand distribution p-value = 7.80e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422283‑422580
                                                                                                                                                     |                                                                                                                                                    
  gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGTTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                                   <  7:1327314/151‑1 (MQ=255)
  gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcctccg                                                                                                                                                   >  2:938914/1‑150 (MQ=255)
   cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcctcctc                                                                                                                                                  >  7:931933/1‑151 (MQ=255)
       gATTGGTGTGCTGTAGATTGGCGGGATGGTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                              <  4:891632/151‑1 (MQ=255)
           gttgtGCTGTTGATTGGCTGGATGTTTAAACTCCGCCACGATCGTCAGCTGTCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                          <  4:1104197/151‑1 (MQ=255)
           gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                          <  1:1217443/151‑1 (MQ=255)
           gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                          <  1:1399424/151‑1 (MQ=255)
             tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                         <  1:288469/150‑1 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                       <  3:734500/151‑1 (MQ=255)
                      gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGTCTGAAAACCATTAATGCTTGCGGTCTGTAAGCCGGTTCATGATGAAATCCTGATCCTGCTATTCAGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                               <  1:104867/151‑1 (MQ=255)
                       aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGACGGTTCAGGAGGAAATCCTGATCCCGCTATTCCGAAAGTTATCCGCCCCCGTCATCCGCCCCCCAATATACTTCCgatg                                                                                                                              >  6:424479/1‑151 (MQ=255)
                         tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                             <  2:1399203/150‑1 (MQ=255)
                         tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCACAATCCTTCAgatgat                                                                                                                            >  5:580313/1‑151 (MQ=255)
                          ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                           <  5:122890/151‑1 (MQ=255)
                             tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGGCGGCTCCCCCCAATATCCTGCACTTgaggagg                                                                                                                        >  2:1608432/1‑150 (MQ=255)
                              ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCATCACAGGATGAGTg                                                                                                                       >  7:1253241/1‑151 (MQ=255)
                              ggATGGTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                         <  6:924414/149‑1 (MQ=255)
                               gATGTTTAAACGGCGCCACGATCGTCTGCTGGCTGAAAACCAGTAATCCTAGCGTTCTGTCAGCCGGTTCAGTAGGAAATCCTGATCCGGCTATTCCGAAAGCTATCCGGCCCCGTCCTCCTCCCCCAAATAACCTTCAGATGATGAGTGa                                                                                                                      <  5:1587368/151‑1 (MQ=255)
                                    ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGGCGGCCTCCGCCCCCAACTATAGGTGAGATGATGAGTGAACCTg                                                                                                                 >  4:1283377/1‑151 (MQ=255)
                                    ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                  <  3:1440285/150‑1 (MQ=255)
                                      aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                                <  7:1563941/150‑1 (MQ=255)
                                        aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCGCCCCCACATATCCTGCAGATGAGGAGTGATCCTGCAtt                                                                                                             >  2:871334/1‑151 (MQ=255)
                                             gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCt                                                                                                        >  4:173156/1‑151 (MQ=255)
                                               cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGGCCCCACATATCCTTCAGATGATGCTTGGTGCTGCAGTAGGCttt                                                                                                      >  3:1146180/1‑149 (MQ=255)
                                                  gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATAACCGTCAGATGATGAGTGATCGTGCAGTTGGGGATGGc                                                                                                   >  2:84133/1‑151 (MQ=255)
                                                      gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCATTAAGATGATGAGTGATGCGGCATTAGGCTATGGCAAgg                                                                                               >  4:698299/1‑151 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCCATATCCGTCAGATGATGAGTGATCCGGCATTAGGCTATGGCAAGGTg                                                                                             >  6:106194/1‑151 (MQ=255)
                                                          gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                           <  5:1005423/151‑1 (MQ=255)
                                                           ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCGCCGCCCCCAAAAATCCTTCAGAAGATGAGGGAGCCTCCATTAGCGACTGGCAAGGTGATc                                                                                          >  8:645425/1‑151 (MQ=255)
                                                             ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                          <  2:275780/149‑1 (MQ=255)
                                                               ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATAACCCTCACATGATGAGGGAGACTGCATTAGGCTATGGCAAGGTGATc                                                                                          >  6:97850/1‑147 (MQ=255)
                                                                 gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAttt                                                                                    <  6:376666/151‑1 (MQ=255)
                                                                  aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                   <  2:408145/151‑1 (MQ=255)
                                                                            aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                         <  3:1287048/151‑1 (MQ=255)
                                                                              ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                        <  3:266625/150‑1 (MQ=255)
                                                                              ccTTGCGGTCTGTAAGCCGGTTCAGGAGGAACTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                        <  1:1282046/150‑1 (MQ=255)
                                                                               cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGGCGCCCCCACAAAAACAGCACAAGATGAGTGATCCGGCATAAGGGTATGGCAAGGTGATCAGAttttgtagactggggc                                                                       >  7:359407/1‑138 (MQ=255)
                                                                                ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAAAATCCTTCAGATGATGAGTGAGCGTGGAGTAGGGGGTGGCAAGGGGGTGTGATTTTGTTCAGAGGGGatt                                                                     >  8:1485851/1‑149 (MQ=255)
                                                                                 tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCGCCTCCCCCAAATATAATTCAGAAGATGATTGATACTGCATTAGTTAATGGAAAGGTGATGATATTTTAATCAAATGCGAAtt                                                                    >  4:152643/1‑151 (MQ=255)
                                                                                  gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATTAGTGATCCTGCATTAGGCTATGGCAATGTGATCAGATTTTCATCACAGGGTAAtt                                                                    <  8:1087180/150‑1 (MQ=255)
                                                                                   cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGGCCCCAAATATACTTCAGATGAGGAGTGATGCTGCGTTAGGAGATTGCACGGGTAGCGGATGTTGATCAAGCGGGAATTat                                                                  >  1:742/1‑151 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGAGCAGATTTTCATCAAACGGGAATTatt                                                                 >  4:438657/1‑150 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGCTGATGAGGGGTGCGGCGGGTGGGTATGGGCAGGTGATCAGATTTTGTTCACAggggctttttg                                                                 >  6:477589/1‑144 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAGTAGGCGATGGCGAGGTGATGAGATGTTCATGACAGGGGAATTttg                                                                 >  4:1371092/1‑151 (MQ=255)
                                                                                     ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgt                                                                >  5:530693/1‑150 (MQ=255)
                                                                                      tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                                <  8:1395849/150‑1 (MQ=255)
                                                                                        tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGGGGGGGCGGCCGGCCACGATCCTGCAGAGGATGAGTGATCCTGCATTTGGCGTTGGCAAGGTGGTGCGATTTTCTTAACAGGGGAATTATGATGt                                                             >  5:905168/1‑151 (MQ=255)
                                                                                         gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                            <  4:1055264/151‑1 (MQ=255)
                                                                                         gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                            <  8:735953/151‑1 (MQ=255)
                                                                                         gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                             <  4:346028/150‑1 (MQ=255)
                                                                                         gTAAGACGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                            <  1:845367/151‑1 (MQ=255)
                                                                                         gTAACCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                            <  8:95704/151‑1 (MQ=255)
                                                                                          tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCGCCCGGCCTCCACACGCCAAGATCCTACAGAAGATGAGTGAACAGGCACTAGGCTATCGCAAGTATCTCAGATATTATTGACAGGGGAATTatcaattt                                                            >  1:923343/1‑144 (MQ=255)
                                                                                            aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAGCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                         <  5:48008/151‑1 (MQ=255)
                                                                                             gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCCAAAATCCGTCAGATGATGAGTGATCCTGCATTAGGCTTTGGCAAGGTGATCAGATTTTGATCACAGGGGATTTATGATGTTAAAt                                                        >  6:1083052/1‑151 (MQ=255)
                                                                                              ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                       <  5:1449872/151‑1 (MQ=255)
                                                                                               cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCGTCAGATGATGAGGGGTCCTGCATTAGGGTTTGGCAAGGTGAGGAGATTTTCATCACACGGGAATTATGTTGTTAAATGc                                                      >  7:114211/1‑151 (MQ=255)
                                                                                                ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCATCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                     <  3:394784/151‑1 (MQ=255)
                                                                                                   tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                   <  5:1259726/150‑1 (MQ=255)
                                                                                                      ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAc                                               >  5:441399/1‑151 (MQ=255)
                                                                                                       gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGGCAGGTGTTCAGATTTTGATCACAGGGGAATTATGATGTTAAATTCATGGCAcc                                              >  6:724876/1‑151 (MQ=255)
                                                                                                        aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                             <  5:367510/151‑1 (MQ=255)
                                                                                                        aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                             <  8:517026/151‑1 (MQ=255)
                                                                                                         ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGTTGCTGGATTAGGCTATGGCAAGGTGATGAGATTTTCATCACAGGGGAATTTTGATGTTAAATGCATGGCCCCTg                                            >  3:165661/1‑151 (MQ=255)
                                                                                                          gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCTCCCCCAAAAATACATAATATGATGAGTGAACATGCATTAGTCATTGGGATGGTGAGCAGATTTTAAAGAAAGGGGAATTATGATTTTAATTGCATGTCACCTGc                                           >  1:1014212/1‑151 (MQ=255)
                                                                                                          gaAATCCTGATCAGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                           <  2:1493937/151‑1 (MQ=255)
                                                                                                            aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGTCCTCGTCCCCCAAAAATCCTTCAGAAAATGAGTGAGACGCGATTAGGCTATGGGAAGGGGAACAGATTTTAATAACAGGGGAATTATGATGTTAAATTAAAGGCACCTGCCg                                         >  7:616523/1‑151 (MQ=255)
                                                                                                            aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCg                                         <  3:302700/151‑1 (MQ=255)
                                                                                                             aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                        <  8:271210/151‑1 (MQ=255)
                                                                                                              tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTTGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTAAATTCATTGCACCTGCCgcg                                       >  5:835136/1‑149 (MQ=255)
                                                                                                               ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATACTTGAGATGATGAGTGATCCTGCATTAGGCGATGGGAAGGTGATCAGATTTTGATGACAGGGGAATTATGATGTTAAATGAATGGCACCTGCCgggg                                      >  4:567820/1‑149 (MQ=255)
                                                                                                                 tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATGGTGCATTAGGATATGGCAAGGTGATCAGATTTTCTTCACAGGGGAATTATGATGTTAAATGAATGGCACCTGCCGGTggc                                    >  3:897024/1‑149 (MQ=255)
                                                                                                                  gATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCGCCCCCAAATAACAGTCAGATTATGAGTGATCCTGCATTAGGGAATGGCAAGGTGCTCCGATTTTCATAACAGCGCATTTATGTTGTTAAATGCATGCCCCGAGCCGGTGccc                                   >  5:1534162/1‑151 (MQ=255)
                                                                                                                   aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCATCAGATGATGAGTGATCCTGCATTATGCTATGGCAAGGTGATCAGATTTTATTAACAGGGGAATTATGATGTTAAATGCATGGAACCTCCCGGGGccgc                                  >  8:1539446/1‑149 (MQ=255)
                                                                                                                      cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                               <  5:1307189/151‑1 (MQ=255)
                                                                                                                       ggCTATTCCGAAAGTTATCCGGGCCCGGCCGCCGCGCCCAAATATACTTAAGATGATGAGTGATGCTGGATTAGTGTATGGGAAGGGGATCAGATTTTAATAACAGGGGAATTATGATTTTAAATGAATGGAAGATGCCGGGGCCCCCAtt                              >  1:574208/1‑151 (MQ=255)
                                                                                                                       ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCATGAAGATTATGAGTGATCGTGCATTAGGCTATGGCAAGGTGAGAGGATTTAAAGAACAGGGGAATTAGAATTTTAAATGAATGCCACATGACGGTGCCCCCAtt                              >  8:895975/1‑151 (MQ=255)
                                                                                                                          tATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCAATATGCTATGGCAAGGTGATCAGATTTTAATCACACGGGAATTATTATTTTAAATTCATGGCACCTGCCCGTGCCCCCATTTGt                           >  2:961793/1‑151 (MQ=255)
                                                                                                                          tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTAAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATGATGTTTAATGAATGTCACCTGCCGGTGCCCCCATTTGt                           >  1:1472581/1‑151 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                          <  5:276491/151‑1 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                          <  3:1332919/151‑1 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                          <  3:1032010/151‑1 (MQ=255)
                                                                                                                            atCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                         <  3:64672/150‑1 (MQ=255)
                                                                                                                             tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                         <  2:515355/150‑1 (MQ=255)
                                                                                                                             tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                         <  6:808757/150‑1 (MQ=255)
                                                                                                                                   aGTTCTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                  <  4:387920/151‑1 (MQ=255)
                                                                                                                                       aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa              <  7:762470/151‑1 (MQ=255)
                                                                                                                                       aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa               <  7:293740/150‑1 (MQ=255)
                                                                                                                                         ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag             <  3:587225/150‑1 (MQ=255)
                                                                                                                                          cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt           <  7:571131/151‑1 (MQ=255)
                                                                                                                                           gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAAAAAGCAAAGAt           >  5:676588/1‑150 (MQ=255)
                                                                                                                                            cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc          >  4:660818/1‑150 (MQ=255)
                                                                                                                                            cccccGACCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa         <  2:1300231/151‑1 (MQ=255)
                                                                                                                                             ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa        <  3:7821/151‑1 (MQ=255)
                                                                                                                                              cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc       >  6:220530/1‑151 (MQ=255)
                                                                                                                                               ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt      <  8:202232/151‑1 (MQ=255)
                                                                                                                                                cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg     <  6:1403497/151‑1 (MQ=255)
                                                                                                                                                   cctcctccCGCAAATATAGTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc  <  4:292750/151‑1 (MQ=255)
                                                                                                                                                   cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc  <  2:1229725/151‑1 (MQ=255)
                                                                                                                                                   cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc  <  2:1342831/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTC  >  NC_000913/422283‑422580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑