Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4350TG24.8% 668.2 / 57.1 335intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (82/167);  new base G (82/0);  total (167/168)
Fisher's exact test for biased strand distribution p-value = 9.57e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  NC_000913/422285‑422583
                                                                                                                                                      |                                                                                                                                                    
gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGTTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                                      <  7:1327314/151‑1 (MQ=255)
  gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcctcctcc                                                                                                                                                    >  6:1514029/1‑151 (MQ=255)
    ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATGCGCGCCCGGCCGCcccccc                                                                                                                                                  >  3:497016/1‑151 (MQ=255)
    ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTcccc                                                                                                                                                  <  5:21100/151‑1 (MQ=255)
     gATTGGTGTGCTGTAGATTGGCGGGATGGTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                                 <  4:891632/151‑1 (MQ=255)
         gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                              <  3:298308/150‑1 (MQ=255)
         gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                             <  1:1217443/151‑1 (MQ=255)
         gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCaaa                                                                                                                                              >  8:838385/1‑150 (MQ=255)
            gtgCTGTTGATTGGGTGGATGTTTAAACGGCGGCACGATCGTCAGCTGGCTGAAAACCAGTAATGCTTGCGTTCTGTAAGCCTGTTCAGGAGGAAATCCTGATCGGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                          <  2:1535444/151‑1 (MQ=255)
            gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCGGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                          <  8:1450104/151‑1 (MQ=255)
            gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATc                                                                                                                                          >  6:401347/1‑151 (MQ=255)
                    gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGTCTGAAAACCATTAATGCTTGCGGTCTGTAAGCCGGTTCATGATGAAATCCTGATCCTGCTATTCAGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                                  <  1:104867/151‑1 (MQ=255)
                        ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                              <  2:888916/151‑1 (MQ=255)
                        ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                              <  5:122890/151‑1 (MQ=255)
                         gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                             <  3:235100/151‑1 (MQ=255)
                           tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTGCCCCCAAAATCCTTCAGaggatgcgg                                                                                                                           >  3:106842/1‑148 (MQ=255)
                            ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCATCACAGGATGAGTg                                                                                                                          >  7:1253241/1‑151 (MQ=255)
                             gATGTTTAAACGGCGCCACGATCGTCTGCTGGCTGAAAACCAGTAATCCTAGCGTTCTGTCAGCCGGTTCAGTAGGAAATCCTGATCCGGCTATTCCGAAAGCTATCCGGCCCCGTCCTCCTCCCCCAAATAACCTTCAGATGATGAGTGa                                                                                                                         <  5:1587368/151‑1 (MQ=255)
                              aTGTTTAACCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                        <  3:1432856/151‑1 (MQ=255)
                              aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGGCGCGGCGCCCCACTAGACGTCAGATGATGAGTg                                                                                                                          >  4:759908/1‑149 (MQ=255)
                                   tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGGCCCCACATATCCTTCAGATCCTGAGGGATCCTGc                                                                                                                   >  5:1288790/1‑151 (MQ=255)
                                   tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                    <  2:1280458/150‑1 (MQ=255)
                                    aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                                   <  7:1563941/150‑1 (MQ=255)
                                     aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCCAAGATCCAGCAGATGATGTGTGAGCCTGcct                                                                                                                 >  3:63235/1‑149 (MQ=255)
                                      aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                                >  1:1611528/1‑151 (MQ=255)
                                        gccgccACGATCGTCAGCTTGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                              <  2:515845/151‑1 (MQ=255)
                                           gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTCCGCCCCCAAATATCCTGCAGATGATGATTGATACTGCATTAgggt                                                                                                           >  6:1348856/1‑149 (MQ=255)
                                            ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                          <  3:990394/151‑1 (MQ=255)
                                              aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                        <  8:614595/151‑1 (MQ=255)
                                               cGATCGTCAGCAGGCTGAAAACCAGTAATCCTTGCGTTCTGTAACCCGGTTCAGGACGATATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCACCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                        <  4:1084793/150‑1 (MQ=255)
                                                gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCACTATCCTTCAGATGATGAGTGATCCTGCGTTTGGCTATGGc                                                                                                      >  6:354187/1‑151 (MQ=255)
                                                 aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                     <  3:1548105/151‑1 (MQ=255)
                                                 aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGCAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                     <  3:674344/151‑1 (MQ=255)
                                                      caccttgctttaaaaCATTAATTCTTTCGTTCTTTAATCCGGTTCATGCTGTAATCCTTTTCCGGGTTTTTCGAAACTTATTCGGCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                                 <  8:916192/139‑1 (MQ=255)
                                                          gggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                            <  2:1468778/150‑1 (MQ=255)
                                                           ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                             <  2:491898/149‑1 (MQ=255)
                                                           ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                             <  8:732936/149‑1 (MQ=255)
                                                             ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATAACCCTCACATGATGAGGGAGACTGCATTAGGCTATGGCAAGGTGATc                                                                                             >  6:97850/1‑147 (MQ=255)
                                                               gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCCAATATCCTTCAGATGATGAGTGATCGTGCATTAGGGGATGGGAAGGTGCTCAGAgtt                                                                                       >  8:20926/1‑151 (MQ=255)
                                                                aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAttt                                                                                       <  6:881851/150‑1 (MQ=255)
                                                                      aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcac                                                                                <  6:104489/151‑1 (MQ=255)
                                                                        tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCGGCATTAGGGTATGGCAAGGTGATCAGATTTTGATcac                                                                                >  4:381013/1‑149 (MQ=255)
                                                                           accTTGCGTTCTGTAAGCCGGTTCAGGAGGAAAGCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                           <  5:1074602/150‑1 (MQ=255)
                                                                            ccTTGCGGTCTGTAAGCCGGTTCAGGAGGAACTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                           <  1:1282046/150‑1 (MQ=255)
                                                                             cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGACGCCCCCAAATATCATGAAGAGTAGGAGTTATCATGAATTAGTATATGGCAACGTGATCCGATTTTCATaacaagtgat                                                                         >  2:1387886/1‑145 (MQ=255)
                                                                             cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGGCGCCCCCACAAAAACAGCACAAGATGAGTGATCCGGCATAAGGGTATGGCAAGGTGATCAGAttttgtagactggggc                                                                          >  7:359407/1‑138 (MQ=255)
                                                                              ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCGCCCAAATATCCGTCAGAGGAGGAGTGATCCTGCATTAGGCTATGGCAAGGGGCTCAGATTTTGATGACAGGGGGAt                                                                        >  1:832361/1‑151 (MQ=255)
                                                                                 cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                     <  3:1252872/151‑1 (MQ=255)
                                                                                 cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCGTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTGATAACAGGGGTAGTat                                                                     >  8:3102/1‑151 (MQ=255)
                                                                                  gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGAGCAGATTTTCATCAAACGGGAATTatt                                                                    >  4:438657/1‑150 (MQ=255)
                                                                                   ttCTGTAAGCGGGTTCAGGAGGAATTCCTGATGCGTCTATTCCGAAAGTTATCCGCGCCCGTCCTCCTCCCCCAAATATCGTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGTATTatg                                                                    <  3:387074/150‑1 (MQ=255)
                                                                                   ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgt                                                                   >  5:530693/1‑150 (MQ=255)
                                                                                     cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGCCGCGGCCCCCAAATATCCTTCAGATGATGAGTGGTCCTGCGGTTGGGTATGGCGAGGGGATCTGGTTTTGCTGCCAGGGGAATTatgttg                                                                 >  5:1514257/1‑151 (MQ=255)
                                                                                      tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGGGGGGGCGGCCGGCCACGATCCTGCAGAGGATGAGTGATCCTGCATTTGGCGTTGGCAAGGTGGTGCGATTTTCTTAACAGGGGAATTATGATGt                                                                >  5:905168/1‑151 (MQ=255)
                                                                                       gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                                <  4:346028/150‑1 (MQ=255)
                                                                                         aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTCCACCCCCAAATATCATTCAGAAGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATGATGttt                                                              >  4:1370521/1‑149 (MQ=255)
                                                                                         aaGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                              <  4:1115684/150‑1 (MQ=255)
                                                                                           gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCCAAAATCCGTCAGATGATGAGTGATCCTGCATTAGGCTTTGGCAAGGTGATCAGATTTTGATCACAGGGGATTTATGATGTTAAAt                                                           >  6:1083052/1‑151 (MQ=255)
                                                                                            ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                          <  5:1449872/151‑1 (MQ=255)
                                                                                            ccGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                          <  7:992321/151‑1 (MQ=255)
                                                                                             cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                         <  3:799262/151‑1 (MQ=255)
                                                                                             cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCGTCAGATGATGAGGGGTCCTGCATTAGGGTTTGGCAAGGTGAGGAGATTTTCATCACACGGGAATTATGTTGTTAAATGc                                                         >  7:114211/1‑151 (MQ=255)
                                                                                               gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                       <  4:1466869/151‑1 (MQ=255)
                                                                                                     gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                                 <  1:659979/151‑1 (MQ=255)
                                                                                                      aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                                <  6:461470/151‑1 (MQ=255)
                                                                                                      aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAAATGATGAGAGATACTGAATTAGGCTATGGCAAGGTGATCAGAATTTGATCACACGGGAAATATGAATTTAAATGAAttcccca                                                 >  4:550262/1‑144 (MQ=255)
                                                                                                        gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCTCCCCCAAAAATACATAATATGATGAGTGAACATGCATTAGTCATTGGGATGGTGAGCAGATTTTAAAGAAAGGGGAATTATGATTTTAATTGCATGTCACCTGc                                              >  1:1014212/1‑151 (MQ=255)
                                                                                                          aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAAAATACATCAGATGATGAGAGATACTGCATTATTCTATGGCAAGCTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGAATGGCACCTGccc                                            >  2:852660/1‑150 (MQ=255)
                                                                                                            tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATGCTTCAGATGATGAGTGGTCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTTATAACAGGGGAATTATTATGTTAAATGCATGGCACCTGCCggg                                          >  5:395427/1‑150 (MQ=255)
                                                                                                               tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATAATTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTTTGATTTTAAATTCATGGCCCCAGCCGGGGcc                                       >  7:682417/1‑151 (MQ=255)
                                                                                                                   ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccccc                                    <  8:1274629/150‑1 (MQ=255)
                                                                                                                   ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATCATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                                   <  8:1557305/151‑1 (MQ=255)
                                                                                                                     ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGATTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCCtt                                 >  5:471280/1‑151 (MQ=255)
                                                                                                                     ggCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTAAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATTCATGTCACCTGCCGGGGCCCCCAtt                                 >  5:821823/1‑151 (MQ=255)
                                                                                                                      gCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATACGTAAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATAAAAGGGGAATTATGATGTTAAATGAATGGCACCTGCCGGTGGCCCCAtgt                                >  1:1462577/1‑149 (MQ=255)
                                                                                                                         aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                               <  8:150884/149‑1 (MQ=255)
                                                                                                                         aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                             <  5:340034/151‑1 (MQ=255)
                                                                                                                         aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                              <  2:94844/150‑1 (MQ=255)
                                                                                                                          ttCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCCAATATCCTTCAGAAGAAGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGAATGGCAACTCCCGGAGCCCCCAATTGtt                             >  5:580289/1‑150 (MQ=255)
                                                                                                                           tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                            <  6:808757/150‑1 (MQ=255)
                                                                                                                           tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                            <  3:1314176/150‑1 (MQ=255)
                                                                                                                              gAAAGTTATCCGCCCCCGTCGGCCTCCCCCACATATCGTTCAGATGATGAGAGATCGTGCATTAGGCTATGGGTAGGTGATGAGATTTTGATAACAGGGGTATTATGATGTTTATTGAATTGCACGTGCCGGTGGGCCCATTTGTTAAAc                         >  1:882661/1‑150 (MQ=255)
                                                                                                                                     aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  <  6:614493/150‑1 (MQ=255)
                                                                                                                                     aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  <  7:293740/150‑1 (MQ=255)
                                                                                                                                     aTCCGCCCCCGGCCTCCTCCCCCAAATAACCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTAATCACAGGGCAATTATTATGTTAAATTCATGGCACCTGCCGGTGCCCCCATTTGTTTAAcaaagaaa                  >  7:768540/1‑150 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa               <  1:932735/151‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGGCCACCTCCCCCAAAATTGCATCACGAGATTAGGGAAGCTGCATTAGGCTTAGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa               <  2:341999/151‑1 (MQ=255)
                                                                                                                                        cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt              <  7:571131/151‑1 (MQ=255)
                                                                                                                                         gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAAAAAGCAAAGAt              >  5:676588/1‑150 (MQ=255)
                                                                                                                                          cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc             >  4:660818/1‑150 (MQ=255)
                                                                                                                                           ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa           <  3:1005812/151‑1 (MQ=255)
                                                                                                                                            cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTATGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc          <  4:280024/151‑1 (MQ=255)
                                                                                                                                            cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc          >  1:355755/1‑151 (MQ=255)
                                                                                                                                            cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc          >  4:165679/1‑151 (MQ=255)
                                                                                                                                            cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc          >  2:196737/1‑151 (MQ=255)
                                                                                                                                                 cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt      <  3:777609/150‑1 (MQ=255)
                                                                                                                                                 cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc     <  2:1335458/151‑1 (MQ=255)
                                                                                                                                                    cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa    <  8:477346/149‑1 (MQ=255)
                                                                                                                                                    cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAtt  <  5:1279347/151‑1 (MQ=255)
                                                                                                                                                    cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAt   <  1:1345775/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  NC_000913/422285‑422583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑