Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,947,4650T.26.9% 381.2 / 154.0 157intergenic (+2/‑32)metZ/metWtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (23/91);  new base . (2/40);  total (25/132)
Fisher's exact test for biased strand distribution p-value = 2.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

Only 100 of 168 total aligned reads displayed.

GCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGG  >  NC_000913/2947316‑2947603
                                                                                                                                                     |                                                                                                                                           
gCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACTCGAGCGGAGTATAGTTCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtt                                                                                                                                            <  1:1282800/151‑1 (MQ=255)
gCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtt                                                                                                                                            >  6:486673/1‑151 (MQ=255)
gCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                                                                             <  5:1389441/150‑1 (MQ=255)
 ccAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtt                                                                                                                                            >  6:40134/1‑150 (MQ=255)
 ccAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAta                                                                                                                                            >  8:181788/1‑149 (MQ=255)
  cAAATCGGGGAGCGGCGTAAAAAAACGGTGGCATGAAAACGCGAGCGGAGTATTGAGGGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                                                                                          <  3:367129/151‑1 (MQ=17)
  cAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTat                                                                                                                                          >  6:210088/1‑150 (MQ=255)
  cAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaa                                                                                                                                          <  8:1415921/151‑1 (MQ=255)
  cAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaa                                                                                                                                          <  8:1324247/151‑1 (MQ=255)
   aaaTCGGTGATCTGGTTATAATAACGGTTGAATGAAAACTCGAGCGGAGTTTAGTGGGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTatt                                                                                                                                         <  7:730193/151‑3 (MQ=17)
   aaaTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTat                                                                                                                                          >  8:1047134/1‑149 (MQ=255)
    aatgtgTGATCTGGTTAAAAAAAAGGTTGCATGAAATCTCGAGCGGAGTATAGTTCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaat                                                                                                                                        <  1:837490/146‑2 (MQ=18)
    aatgcctGATCGGGGTAAAAACAAGGATGCATGAAAACGCCAGCGGAGTAGAGTCCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaat                                                                                                                                        <  2:1493113/145‑2 (MQ=18)
    aatcgctGATCGTGGTAACAATAGGGTTGAATGAAAACCAGATCGGAGTATAGTGGGAATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaat                                                                                                                                        <  2:856483/145‑2 (MQ=14)
     atgcGTGATCTGGGTAAAAAAAAGGTTGCATGAAAACTCGAGCGGAGTATATTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaatt                                                                                                                                       <  7:1245695/148‑3 (MQ=18)
     atgcGTGATCGGGGTAAAAAAAAGCGTGAATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaatt                                                                                                                                       <  2:1228838/148‑3 (MQ=21)
     aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattg                                                                                                                                        >  2:870617/1‑147 (MQ=255)
     aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt                                                                                                                                       <  3:1496430/151‑1 (MQ=255)
     aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt                                                                                                                                       <  4:806754/151‑1 (MQ=255)
     aTCCGGGATGGGGGTAAAAATAAGGTTGCATGAAAACGCGAGGGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaatt                                                                                                                                       <  4:125990/151‑3 (MQ=21)
      gccGGGAGCGGCGTAAAAACACGGTTGCACGAAAACGCCAGCGGAGTATACACCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt                                                                                                                                      <  2:1489628/150‑1 (MQ=17)
       ggggtaTCGGGTTAAAAAAAAGGTTGAATGAAAACTCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt                                                                                                                                      <  1:1188524/145‑1 (MQ=18)
       gcGTGATCGGGGTAAAAAAAAGGTTGCATGAAAAAGCGAGCGGAGTATAGTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaac                                                                                                                                     <  4:602130/150‑7 (MQ=255)
       ccGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAtt                                                                                                                                      <  5:86052/150‑1 (MQ=255)
       ccGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAtt                                                                                                                                      <  4:362042/150‑1 (MQ=255)
        ggTGATCGGGGTAAAACAAAGGGTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                                                                                   <  5:249368/150‑1 (MQ=18)
        cGTGATCGGGGTAAAAACCACGTTCAATGAAAACCCCATCGGAGTATACTGCGAATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                                                                                   <  3:1573447/151‑1 (MQ=14)
        cGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaac                                                                                                                                     >  6:3925/1‑144 (MQ=255)
        cGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGAAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGCTTCAAATCACGCCACCGCAACCAATTatagaaa                                                                                                                                     >  1:1044781/1‑144 (MQ=25)
         gTGATCGGGGTATAAACAAGCTGGCATGAAACCGCGAGCGGAGTATAGTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacac                                                                                                                                   <  3:1277258/151‑9 (MQ=17)
          tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTttatt                                                                                                                                 >  7:212377/1‑150 (MQ=255)
           gagctggttataaacaaGGCTGAAAGAAATCCCCATCGGAGTATAGACGGCCTCCCGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgatga                                                                                                                                <  2:721689/140‑1 (MQ=2)
           gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacacgc                                                                                                                                 >  5:1216207/1‑141 (MQ=255)
           gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacaccc                                                                                                                                 >  6:1279553/1‑141 (MQ=255)
           gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatg                                                                                                                                 <  2:409226/151‑1 (MQ=255)
            aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacaccct                                                                                                                                <  5:1477727/151‑12 (MQ=255)
            aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatg                                                                                                                                 <  8:358017/150‑1 (MQ=255)
             tCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacaccct                                                                                                                                >  8:589049/1‑139 (MQ=255)
             gcGGGGTAAAAAAAAGGTTGAAGGAAAACCCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacacccta                                                                                                                               <  3:604645/150‑13 (MQ=17)
              cGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGCGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacaaggatg                                                                                                                              >  2:63763/1‑138 (MQ=255)
              cGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGaa                                                                                                                               <  4:102239/150‑1 (MQ=255)
              cGGGGAAAAAAAAAGGTTGCACGAAAACGCGAGCGGAGTATAGAGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacacccta                                                                                                                               <  3:597388/150‑13 (MQ=17)
               ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCAGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑a                                                                                                                           <  5:1067154/151‑1 (MQ=255)
               ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAGGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                                                                             <  5:1382182/151‑1 (MQ=255)
               ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacaccctaac                                                                                                                             <  3:86182/151‑15 (MQ=255)
               ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                                                                             <  6:1389199/151‑1 (MQ=255)
               ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                                                                             <  2:933112/151‑1 (MQ=255)
                gggTAAAAATAAGGTTGCATGAAATAGATATGGGAGTATATTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aa                                                                                                                          <  1:958241/151‑1 (MQ=14)
                gggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑a                                                                                                                           <  7:1346386/151‑1 (MQ=255)
                gggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑a                                                                                                                           <  1:1016392/151‑1 (MQ=255)
                 ggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGGGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑aa                                                                                                                          <  7:611442/151‑1 (MQ=255)
                  gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑aaa                                                                                                                         <  3:964792/151‑1 (MQ=255)
                   tataattaaGGTTGCATGAAATCGAGAGTGTTGTATTCTTGGCTTCCTCGGACGGGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaa                                                                                                                        <  1:1565964/143‑2 (MQ=11)
                   tAAAAAAAAGGTTGCATCAAAAAGAGTGCGGAGTATAGTCCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑a                                                                                                                           <  6:532396/147‑1 (MQ=17)
                   aaaaagacaGGTGGAAGGAAAAAGCCAGCGGAGTATTGTCCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaag                                                                                                                       <  3:1447510/150‑3 (MQ=14)
                     ataattaGGTTGGATGAAAAGGCGAGCGGAGTATAGTGCGGATCGTGGGACGCGGGGTGGAGCAGCGTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAGCAATTAAAATTTGATGATGT‑AAAgga                                                                                                                      <  8:773549/145‑3 (MQ=17)
                     aaaaaacGGTTGCATGAAAATCCCAGCGTAGTATTTTGGGATTCCCGGGACGGGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaagca                                                                                                                     <  4:1498171/151‑5 (MQ=1)
                     aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCa                                                                                                                      <  4:696639/151‑1 (MQ=255)
                      aaatacggTTGCATGAAAACTCGATCGGAGTATACTCGGATTCCCGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaagcag                                                                                                                    <  7:565740/145‑6 (MQ=11)
                      aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAg                                                                                                                     <  6:1271823/151‑1 (MQ=255)
                       ataacggTTGCATGAATAATAGATCGGTGAATTGTGCGCTTCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGt                                                                                                                    <  3:206467/146‑1 (MQ=11)
                        aaaaGGTTGCATGAAAAGGGGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTa                                                                                                                   <  8:207273/151‑1 (MQ=255)
                         aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTAc                                                                                                                  <  8:1043321/151‑1 (MQ=255)
                          ccccgtGCACGAATACCCGTGCGTAGTATACTGCGAATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTa                                                                                                                   <  2:268008/144‑1 (MQ=1)
                           aGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACgg                                                                                                                <  7:539604/151‑1 (MQ=255)
                           aGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACgg                                                                                                                <  2:602869/151‑1 (MQ=255)
                            ggTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattg                                                                                                                                        >  1:1263724/1‑124 (MQ=255)
                            ggTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGt                                                                                                               <  3:786402/151‑1 (MQ=255)
                            ggTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAAATTTCTGCAGGA‑AAAGCAGTACGGt                                                                                                               >  6:1164935/1‑151 (MQ=18)
                            cggtgaaTGAAAAATCGATCGGAGTATACTGCGAATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGt                                                                                                               <  5:782522/145‑1 (MQ=11)
                                gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcg                                                                                                           <  5:457829/151‑1 (MQ=255)
                                gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcg                                                                                                           <  1:334584/151‑1 (MQ=255)
                                gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAc                                                                                                            <  3:1358766/150‑1 (MQ=255)
                                 cATGAAAACGCGAGCGGAGTATAGTGCTCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgc                                                                                                          <  4:1553223/151‑1 (MQ=255)
                                 cATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgc                                                                                                          <  1:634435/151‑1 (MQ=255)
                                  aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgcg                                                                                                         <  2:567333/151‑1 (MQ=255)
                                     aaaaatagAGCGGAGTATAGACCCACTCCGCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgggg                                                                                                      <  6:87182/144‑1 (MQ=11)
                                     aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCgggg                                                                                                      <  2:222926/151‑1 (MQ=255)
                                      aaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGt                                                                                                     <  6:1602393/151‑1 (MQ=255)
                                      aaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGt                                                                                                     <  6:1448963/151‑1 (MQ=255)
                                      aaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGt                                                                                                     <  6:1537633/151‑1 (MQ=255)
                                         cgcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAATTTTTATGAAGA‑AAAGCAGTACGGTGAcgagcgcggc                                                                                                   >  5:202606/1‑142 (MQ=17)
                                         atcgttcGGTGTATTTTGGGCATCCTGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGAcg                                                                                                           <  1:202955/136‑1 (MQ=11)
                                         agagAGGGGAGTATACTGCCATTCCGCGGACGCGGGGTGGAGGAGCCTGGTCGCTCGTCGGTCTCATAACCCTAAGGTCGTCTGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAACTAAAAGCAGTACGGTGACGCGGGGTGGa                                                                                                  <  6:1593014/148‑1 (MQ=11)
                                           cgAGGGGAGTATAGTGCGCATCGAGGGACGCGGGTTGGAGCAGCCTGGTCGGTCGTCGGGATCATAACCCGAAGGTCGTCGGTTCAAATCCGTCCCCCGCAACGAATTAAATTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGTTGGagc                                                                                                <  8:702429/151‑1 (MQ=17)
                                           cgAGCGGAGTATAGTGAGAATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AACGCAGTACGGTGACGCGGGGTGGagc                                                                                                <  8:131221/151‑1 (MQ=255)
                                            gAGCGGAGTTTAGTGCGCATGCACGGTCGCGGGGTGGAGGAGTGTGGTTGCTGGTGGGGCTCATATCCGGAAGGTCGTCGGTGGAAATCCGGCGCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGagca                                                                                               <  7:1033835/151‑1 (MQ=17)
                                             aGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                                                           <  6:821509/107‑1 (MQ=255)
                                                gttgTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGAcgc                                                                                                          <  8:614850/133‑1 (MQ=11)
                                                ggAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTattgaacacc                                                                                                                                  <  3:739530/113‑10 (MQ=255)
                                                ggAGTATACAGCGCCTCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtt                                                                                                                                            <  5:568965/103‑1 (MQ=14)
                                                  aGTATAGTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGAcgcg                                                                                                         <  7:440138/135‑1 (MQ=11)
                                                       aGTGCGCATGCACTGACGCGTGGTGGAGCAGCCTGGTAGCTGGTCGGGCTAATAACCCGAAGGTCGTCGGTTCAAATCCTGCCCCCGCAACGAATTATAATTTGATGAAGT‑AAAGCAGTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCt                                                                                    <  5:1151634/151‑1 (MQ=14)
                                                       aGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt                                                                                    <  3:532092/151‑1 (MQ=255)
                                                        gTGCTCATCCAGGGACGCGGCGGGGAGCTGGCTCGTAGCTCGGCTGTATCATAACCCCAAGGCCGGCGCGGCAAATAAGCCCCCCGCAACCAAATAAAAATTGCTGAAGA‑AACCCACTACCCGGACGCGGGGTGGAGCAGCCTGGTAGCtc                                                                                   <  2:482301/151‑1 (MQ=9)
                                                                ccACGGACGCGGGGTGGAGCAGGGTGGTAGCTCGTCGGTCTCATAACCCGAAGGTCGTCGGTGCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGc                                                                            <  4:260103/150‑1 (MQ=255)
                                                                  cctgactaggtctGGAGGAGCCTTTTAGCTCGTCTGTATATTAAGCCTAATGTCGGCTGTTCAAATAATCCCCCCTCAACCAATTAAAAATTGAAGAAGA‑AACGCACTACCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                         <  3:228636/139‑1 (MQ=2)
                                                                                    agcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                       <  7:1455868/119‑1 (MQ=255)
                                                                                                                      tcgtcgGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTg                                                                                                    >  8:1364390/1‑72 (MQ=255)
                                                                                                                             ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc                                                                    >  5:1036241/1‑97 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
GCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGG  >  NC_000913/2947316‑2947603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑