Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,591	0	A	.	28.7%	198.1 / 82.9	80	intergenic (+18/‑16)	metW/metV	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (51/6);  new base . (21/2);  total (72/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

CCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947453‑2947740                                                                                                                                             |                                                                                                                                                       ccccTCAATCAATTATACTTTGATGAATTAATGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACgg                                                                                                                                            <  5:84834/151‑1 (MQ=18)                  aTTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGt                                                                                                                                                <  6:1313960/130‑1 (MQ=255)                  aTTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCACCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGt                                                                                                                                                >  7:1320650/1‑130 (MQ=255)                                    atcgccggACGCGGTGGGGAGCAGGCTTGTAGCTCTTCGGTATCACATCCCGAATGGCGTCGGGGGCTATCACGCCGCCGCAACCAA‑TAAAACTTTGCAGTAGT‑AACGCACTACCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                                         <  2:654271/144‑1 (MQ=9)                                     tttggtgactatgtttGGAGCAGAGTTGTTGCTCGTCGGTATCATATGCCTAAGGTCGGAGGTTCATATCCTGCCGGCTCAACGAA‑TAAAA‑TATGTTTTTCTACTTGGATTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                                         <  4:264245/135‑1 (MQ=2)                                                                     tcgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAg                                                                                                                                                 <  6:465019/78‑1 (MQ=255)                                                                     tcgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAA‑TTTTATTAAGTAAAAGCAg                                                                                                                                                 >  7:467492/1‑78 (MQ=14)                                                                                aTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TGATA‑TTTCATCAAGTAACAGCAGTAGGTTGACGCGGTGTGCAGAATCCTGGTAGCTAGTCGCGCTCATAACCCGAAGGGCGGCCGTGCAAATCCCGCCCACGCaaa                                                              >  2:733893/1‑149 (MQ=2)                                                                                            tcgtcgGTTCAAATCCGGCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTg                                                                                                                                <  2:1363926/72‑1 (MQ=255)                                                                                                    tCAAATCCGGCCCCCGCAACCAA‑TGAAA‑TTTGAGGAAGTAAGAGCTGTAGGGTGACGGGGTGTGGAGGAGCATGGTAGCTCGGCGGGCTCATTACCCGTAGGTCGGCCGGTCAAATCCGGCCCCCGCAACCAATTTTTTAAAACCCTAACg                                         >  3:46694/1‑151 (MQ=14)                                                                                                     cccctCCCGCCCCCGCAACCAA‑TTAAA‑ATTGCAGCACGAAAAGCAGTACGGTGACGCGGGGGGGAGCAGCCTGGTAGCTCGTCGTGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACgg                                        <  5:862161/147‑1 (MQ=14)                                                                                                          ccGGCCCCCGCAACCAA‑TCAAT‑TTTCATAAAGTAAAAGCTGTAATGTGACGGGGGCTCGCAGAGACAGGAAGCAAGACGGGCTCATAAATCGAACGTAGAACGTTCACCTCCGGCCAACGCAAACAAAAAATTAACACCCaaaatgg                                       >  4:1576673/1‑143 (MQ=255)                                                                                                            ggCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGGGGGGTGGATCAGCCTGGTAGCTCGACGGGCTCATAACCCGAAGGTCGGGGGTTCAACTCCGGCCCCCGCCACCAATTATTGAAAACCCAAACTGGTGt‑ttt                                >  3:1505030/1‑151 (MQ=17)                                                                                                             gCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGt‑tttt                               >  5:800036/1‑151 (MQ=255)                                                                                                              cccccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGt‑ttttt                              >  7:986143/1‑151 (MQ=255)                                                                                                              cccccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCACCCAATTATTGAACACCCTAACGGGTGt‑ttttt                              >  3:650019/1‑151 (MQ=255)                                                                                                               ccccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGt‑tttttt                             >  4:387526/1‑151 (MQ=255)                                                                                                               ccccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGt‑tttttt                             >  5:1133953/1‑151 (MQ=255)                                                                                                               ccccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGt‑tttttt                             >  6:312699/1‑151 (MQ=255)                                                                                                                cccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTg                            >  8:823517/1‑151 (MQ=255)                                                                                                                cccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTg                            >  4:1061762/1‑151 (MQ=255)                                                                                                                 ccGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGt                           >  1:844601/1‑151 (MQ=255)                                                                                                                  cGCAACCAATTAAAA‑ATAAATCAAGT‑AAAGAACTACGGTGACTCGTGGTGGAGAAGACTGGTAGCTAGTCTGGCTAATAACCCGAAGGTCGTATGATAAAATAAAGCCCCCGCAACCAATTATTGAACAGCCTGACgg                                        >  1:862813/1‑138 (MQ=255)                                                                                                                  cGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTggt                          >  4:15577/1‑149 (MQ=255)                                                                                                                  cGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGtt                          >  7:727151/1‑151 (MQ=255)                                                                                                                  cGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGtt                          >  5:1280253/1‑151 (MQ=255)                                                                                                                   gCAACCAATTAAAA‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGTACACCCTCACTGGTGT‑TTTTTTggt                          >  2:1121080/1‑148 (MQ=11)                                                                                                                   gCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGttt                         >  8:842421/1‑151 (MQ=255)                                                                                                                     aaCCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCt                       >  2:1183198/1‑151 (MQ=255)                                                                                                                     aaCCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGCGTGT‑TTTTTTGTTTCt                       >  4:1374256/1‑151 (MQ=255)                                                                                                                      accaattaaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGATCAGCCAGAAGGGTGTGttgtttgtgctg                       >  6:1092899/9‑147 (MQ=11)                                                                                                                      accaattaaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTATCGGGGGT‑TTTTTTGTTTCTg                      >  6:1161380/9‑151 (MQ=14)                                                                                                                      aCCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTg                      >  8:139470/1‑151 (MQ=255)                                                                                                                      aCCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGG‑CCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTgg                     >  3:450718/1‑151 (MQ=255)                                                                                                                       ccAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTgg                     >  4:495264/1‑151 (MQ=255)                                                                                                                        caattaaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACAACCTCACGGCTGT‑GTTTTTGTTTCgggg                    >  3:987187/7‑150 (MQ=11)                                                                                                                        cAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGt                    >  7:1207014/1‑151 (MQ=255)                                                                                                                        cAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGt                    >  6:770578/1‑151 (MQ=255)                                                                                                                        cAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGt                    >  3:913916/1‑151 (MQ=255)                                                                                                                         aattaaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGATTGAAGACCCTGACGGGGGT‑TTTTTTGTTTCTGGtg                   >  5:521876/6‑150 (MQ=14)                                                                                                                         aa‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGtc                   >  2:1450706/1‑151 (MQ=255)                                                                                                                         aa‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGtc                   >  2:567299/1‑151 (MQ=255)                                                                                                                          attaaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATGAACACCAAAAAAGGGCT‑TTTGTTTTTTCAGGtct                  >  8:59112/5‑151 (MQ=1)                                                                                                                          attaaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTGAACAACCTATCGGGGGA‑TTTGTTTTTTCTGGtgt                  >  7:216773/5‑149 (MQ=11)                                                                                                                          a‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGtct                  >  4:500013/1‑151 (MQ=255)                                                                                                                          a‑TCAAA‑TATGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGAAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTACAAATCCGGCCCCCGCAACCAATTAATGAACACCCTAACCGGTGT‑TATTTTGTTTCTGGtct                  >  8:1127942/1‑151 (MQ=18)                                                                                                                            taaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACAACATAAAGGGTGT‑TTTATTTTTACAGGtctac                >  1:1088899/3‑149 (MQ=11)                                                                                                                            taaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGtctc                 <  1:642791/148‑1 (MQ=255)                                                                                                                             cAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAATTCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTcc                >  7:1105607/1‑151 (MQ=255)                                                                                                                             aaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGGGT‑TTTTTTGTTTCGGGTCTccc               >  2:1353815/2‑151 (MQ=255)                                                                                                                             aaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGGGT‑GTTTTTGTTTGTggggtg                 >  5:642495/2‑145 (MQ=14)                                                                                                                             aaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAAAGGGTGT‑TTTTTTGTTTCTGGTCTccc               >  3:1365325/2‑151 (MQ=255)                                                                                                                             aaaa‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATGAACAACCTAACGGCTCT‑TTTGTTTTTTCTGGTCTcc                >  1:837326/2‑150 (MQ=14)                                                                                                                              aaa‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTccc               >  4:1315463/1‑151 (MQ=255)                                                                                                                               aa‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTACCGGGTGT‑TTTTTTGTTTCTGGTCTccc               >  7:862825/1‑150 (MQ=255)                                                                                                                                a‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCAt             >  8:930722/1‑151 (MQ=255)                                                                                                                                a‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACCCTAACGGGTGT‑GTATTTGTTTCTGGTCTCCCAt             >  8:1359259/1‑150 (MQ=255)                                                                                                                                a‑TTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGATTTAACAACCTAAACGGGGT‑TTTGTTGGTTCTGGTCTgccct             >  5:768604/1‑148 (MQ=11)                                                                                                                                  tttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCAt             >  1:1553758/1‑150 (MQ=255)                                                                                                                                  tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACGCTCACGGGTCT‑TTTTTTGGTTGAGGGCTCCCaaa            >  5:1036567/1‑148 (MQ=14)                                                                                                                                  tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATa            >  2:796362/1‑150 (MQ=255)                                                                                                                                  tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTGAACACCCTAACACGGCA‑TTTTTTTTTACAGGTCTCCCATa            >  8:26101/1‑150 (MQ=14)                                                                                                                                  tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTGAACAACCAAACACATCA‑TTTATTGTAACTGGTCTCCCATa            >  7:298360/1‑150 (MQ=11)                                                                                                                                   ttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATa            >  1:374160/1‑150 (MQ=255)                                                                                                                                    tgatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACAACCTAACACAGCA‑TTTTTTGTATCTGGTCTCCAATaaa          >  7:1067062/1‑150 (MQ=14)                                                                                                                                     gatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAAACGGTCT‑TTTTTTGTTTCTGGTCTCCCATaaaa         >  1:642992/1‑150 (MQ=255)                                                                                                                                      atgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATaaaa         >  6:401171/1‑150 (MQ=255)                                                                                                                                      atgaAGTAAAAGCAGTACGGTGACGAGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGCGTGT‑TTTTTTGTTTCTGGTCTCCCATaaaa         >  4:794157/1‑150 (MQ=255)                                                                                                                                       tgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTAAAATCCGGCCCCCGCAACCAATTATTTAACACCCTAACGGGTGT‑TTTTTTGTTTATGGTCTCCCATaaaaa        >  4:336589/1‑150 (MQ=255)                                                                                                                                        gaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATaaaaa        >  5:642620/1‑149 (MQ=255)                                                                                                                                        gaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATaaaaa        >  6:850470/1‑149 (MQ=255)                                                                                                                                        gaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGG‑TTTTTTGTTTCTGGTCTCCCATaaaaa        >  3:568639/1‑149 (MQ=255)                                                                                                                                         aaGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGCCGGGCTCATAACCCGAGGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAgc     >  1:833832/1‑151 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTGTCCCATAAAAAAgcg    >  6:758718/1‑151 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAgcg    >  6:766148/1‑151 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAgcc    >  8:599295/1‑150 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCAGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAgac    >  5:1013493/1‑149 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGATAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAgcg    >  8:64964/1‑151 (MQ=255)                                                                                                                                           gTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAgcgc   >  8:1586514/1‑151 (MQ=255)                                                                                                                                           gTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAgcgc   >  1:1622236/1‑151 (MQ=255)                                                                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  5:1532496/1‑150 (MQ=255)                                                                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  1:637957/1‑150 (MQ=255)                                                                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  4:563309/1‑150 (MQ=255)                                                                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  6:1419015/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                                       CCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAA‑TTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGT‑TTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947453‑2947740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑