Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9940GT25.0% 225.5 / 18.1 164intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (15/90);  new base T (0/35);  total (15/149)
Fisher's exact test for biased strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

TGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCT  >  NC_000913/3269844‑3270131
                                                                                                                                                       |                                                                                                                                          
ataaaCCCGCCGCACCGGTTTATTACGTACATCAGGTTTATTTTAACGATAAGCCGCTTTCTTTTGTGTATAGTTTCGTGGACAGTCATTCATCTTTCTTCGCGTTTAATAGCTTAAACCCGCCGAATC‑GGGTTTTTACGTAAATCAGGTg                                                                                                                                            <  8:913603/149‑1 (MQ=2)
 gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTg                                                                                                                                            <  6:1155198/150‑1 (MQ=255)
  aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGaa                                                                                                                                          <  4:265033/151‑1 (MQ=255)
  aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGaa                                                                                                                                          <  8:702162/151‑1 (MQ=255)
   aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGaaa                                                                                                                                         <  8:315417/151‑1 (MQ=255)
   aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGaaa                                                                                                                                         <  5:294215/151‑1 (MQ=255)
       cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                     <  3:1397556/151‑1 (MQ=255)
       cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                     <  1:48177/151‑1 (MQ=255)
        gccgTATCGGTTTATTTAGTTAATCATTTTTAACTGAACGATAATCCTCTTTTTTTTGGGTATAGTGTCGTGGAAAGTCATTCTTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAATCAGGTGAAACTGAc                                                                                                                                    <  2:1033420/151‑1 (MQ=1)
        gccgGAACGGGTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                                                    <  8:858821/151‑1 (MQ=255)
         ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                   <  3:1479027/151‑1 (MQ=255)
         ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                   <  5:539913/151‑1 (MQ=255)
         ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                   <  5:435639/151‑1 (MQ=255)
          cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                                                  <  1:547704/151‑1 (MQ=255)
            taaCGGTTTATTACGTACATCAGGTAAAACTTACCGATAATCCTCTTTCTTTTTGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                                                                 <  2:1058074/149‑1 (MQ=255)
            gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                                                <  1:1126886/151‑1 (MQ=255)
            gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                                                <  8:1231246/151‑1 (MQ=255)
            gAACGCTTTATTACGTACATCAGGTAACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                                                <  4:1443434/151‑1 (MQ=255)
             aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                                               <  5:184191/151‑1 (MQ=255)
             aaCGGTATATTACGTACATCAGGTAAAACTGACCGATAAGCGGCTTTCTTTTGGGTATAGGGTCGTCGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                                               <  7:1568732/151‑1 (MQ=255)
              aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                              <  5:1498101/151‑1 (MQ=255)
                 gTTTATTACGTACATCACGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                                           <  7:38759/151‑1 (MQ=255)
                    tattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTAGTGCGGAGGCGATCAAGTGTGAACGCCAGAAAAAGTAAAAAACCGCCGAAGC‑CGGGTTGTTCGTTAAAAATGTGAAACTggcggggtggggggg                                                                                                                        >  5:1543743/1‑137 (MQ=9)
                     attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctt                                                                                                                       <  7:263862/151‑1 (MQ=255)
                     attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGct                                                                                                                        <  5:1172053/150‑1 (MQ=255)
                       taCGTACATCATGTAATACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                      <  8:493052/150‑1 (MQ=255)
                       taCGTACATCAGGTAAAACTGGCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                      <  7:1025768/150‑1 (MQ=255)
                        aCGTACATCTTGTAACACTTACCGATAAGCCGCTTTCTTTTTGTTATATTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAATC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttct                                                                                                                    <  1:807749/151‑1 (MQ=14)
                        aCGTACATCAGGTAAAACTGACCGATAAGCAGCTTTCTTTTTTGTATAGTGTCGTGGACAGTGTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGctttc                                                                                                                     <  1:755554/150‑1 (MQ=14)
                        aCGTACATATGTTAAAACTGACCGATATTCGGTTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTATAAACCCGCCGAATC‑GGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGctttct                                                                                                                    <  1:1465301/151‑1 (MQ=11)
                         cGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttctt                                                                                                                   <  7:996324/151‑1 (MQ=40)
                           gtcatcatTTAAAACTGAGCGATAAGCCGCTTTCATTTGGGGCTAGTGTCGGGGACTGTCTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                                 <  5:248028/149‑1 (MQ=11)
                            acctCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTTTCTATAGTGTCGTGGACAGGCATTCCTCTTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                <  3:1197508/148‑1 (MQ=11)
                              ataacgTAAAACTGACCGATAAGCGGCAGGCTTTTGGGGCTAGTGTCGTCGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                                              <  3:571519/146‑1 (MQ=11)
                               acttgttataattagcGATAAGCCGTTTTCTTTTGTTTTTATTGTCGTGGACAGTGTTTCATATTTCTGCCACTCCAAAAGTAAAAACCCGCCGTATC‑GGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                                                                             <  4:1487353/136‑1 (MQ=11)
                                aatgTAAAACTGAGCGATATGCCGCTTTCTTTTTTGTATAGTGTCGTGGAAAGTCATTCATCTTTCTGCCACTCAAAAAGTATAAACCCGCCGAATC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                            <  4:306483/148‑1 (MQ=11)
                                aaGGTAAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                            <  7:451349/150‑1 (MQ=25)
                                  ggTAACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGTACAGTCTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                          <  7:152390/151‑1 (MQ=255)
                                     aaaaCTGAGCGATAAGTCGCTTTCTTTTTTTTATAGTGTCGTGGAAAGTGATTCATCTTTCTGCCCCTCAAAAAGTAAAAACCCGCCGAATC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtg                                                                                                       <  1:360044/151‑1 (MQ=11)
                                     aaaaCTGACCGATCAGCCGCAGGCTTTTGCGGCGCGTGTCGGCGCCAGGCATTCCTCTTTCTGCCACTCCAAAAGGAAAAACCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtg                                                                                                       <  2:1551858/151‑1 (MQ=11)
                                     aaaaCTGACCGATAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTCACTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgt                                                                                                        <  6:1339002/150‑1 (MQ=25)
                                      aaaCTGACCGATAAGGAGCTTTCTTTTTGGTTTAGTGTCGTGGACTGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtgt                                                                                                      <  5:171028/151‑1 (MQ=255)
                                       aaCTGACCGATAAGCCGCTTTCTTTTGCGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc                                                                                                     <  3:1415099/151‑1 (MQ=25)
                                       aaCTGACCGATAAGCCGCTTTCTTTTGCGTATAGTGTCGTGGAAAGTGATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCACC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc                                                                                                     <  3:1127497/151‑1 (MQ=255)
                                        aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCAACGTTGGTCCCCAGCAAAACAAAAAAACAGCCCACCC‑GGCTGTTTAAGTAAAACAGTTGGAAATGACCGCGAAGCCCCGTTCTTTTggggggagggggg                                                                                                    >  5:1397480/1‑141 (MQ=2)
                                         atttccGATAAGCCGCATTCTTTTTTTTATAGTGTCGTGGCAAGTCTTTCATCTTTCTGGCACTCAAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt                                                                                                   <  1:147986/147‑1 (MQ=11)
                                           gACCGATAAGCCGCATGCTTTTGAGTATCGTCTCGCCGACAGGCACTCATCTTTCTCCCACTCAAAAAGTATAAACCCGCCGAACC‑GGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                                  <  3:293928/150‑1 (MQ=11)
                                            agcGATAAGGCGCTGTCTTATGGTTTTTTTCTCGTGGACAGTCCTTGCTCTATCTGCCACTCAAAAAGTACAAACCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑Ga                                                                                               <  2:783339/149‑1 (MQ=11)
                                            aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTAAATGAAGTTTATGCCCCACCAAAACTAAAAAACCCCCCCAGCGGGGTTTTTAC‑TAAAAAACGTGTAAATGACCGAAAAACCGCTTTGATTTggggttaaattactggc                                                                                                >  5:580036/1‑137 (MQ=2)
                                                aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTGTGCCCCTCCAAAAGTAAAAACCCCCCGCAGC‑GGGTTTTTACGGAAATAAGGTGAAACAGAACGATAACCAGGGTTCTTTAGTGTAAAgtgctggtgggagg                                                                                            >  3:832564/1‑140 (MQ=11)
                                                 tatggcgttgtctttAGTGTAACGTGTCGTGGCCAGCGTTTCTTCTTTCTCGCACTCCAAAAGCTAAAACCCGCCGAACC‑GGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGt                                                                                           <  4:1248592/140‑1 (MQ=2)
                                                 tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTGTGCCCCACCAAAACCAAAAACCCGCCGAAGC‑GCGGTTTTACGTAAACCACGTGCAACTGAAACATAACCCGCTTTCTTTTGGGGAAAgtctggtctacaggc                                                                                           >  6:309256/1‑138 (MQ=11)
                                                  aagggggattctttTTGGTCGTGTGTCGTGGCCACTGTATCTTATTTATCGCCCTCCAAAATTAGAAACCCGCCGCACC‑GGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGtca                                                                                         <  7:936301/143‑1 (MQ=2)
                                                  aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCAACAAAACAAAAAACACGCAGAAGC‑CGGGTTTTAAGTAAAAAAGGTGAAACAGACCGATAAACCGCGGTCAGTTGGGTAAAgggacgcagccaggcc                                                                                          >  6:14431/1‑136 (MQ=2)
                                                   aGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTGGGCCCCAACAAAAAGAAAAACCCCCCGAAGC‑CGGTTTTTTCCTAAAAAAGGTGAAACTGACCGATAAGCCGCGTTATTTAGGGTAAAGTGtggtg‑gggagggcg                                                                                        >  5:741550/1‑143 (MQ=2)
                                                       gCTTTCTTTTGGGTATTGTGTTGTGGAATGTGTTTTTTATTTATGGCACTCAAAAATTTTAAACCCGCCGTATC‑GGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGtcattca                                                                                     <  8:436768/150‑1 (MQ=2)
                                                       gCTTTCATTTGCGTCTCGTGTCGCGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCTGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGtcattcat                                                                                    <  2:550820/151‑1 (MQ=11)
                                                          ttctttTGGGTATAGTCTCGTGGAAAGTCATACATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCACC‑GGTTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCt                                                                                  <  3:365457/150‑1 (MQ=11)
                                                            catttttgtttgttgtCGTGGAATGTGTTTCTTATTTCTGCCACTCAAAAATTATAAACCCGCCGAATC‑GGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCttt                                                                                <  4:733557/137‑1 (MQ=11)
                                                              ttattctataGTGTCGCCGACAGCGATTCAACTATCTCCCACTCAAAAAGTACAAACCCGCCGTAGC‑GGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCt                                                                              <  1:187467/144‑1 (MQ=11)
                                                                       tttctCGTGGAATGTCTTTCTTATTTCTGGCACTCAAAAATTTTAAACCCGCCGTATC‑GGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCa                                                                     <  4:1469517/146‑1 (MQ=11)
                                                                        gtcacgccgcatccgattcgtCTTACTGCCCCTCAACAAGTACAAACCCGCCGAAGC‑GGTTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCaaa                                                                   <  2:360277/136‑1 (MQ=11)
                                                                              gggACAGTGATTCATCTTTCTGCCCCTCCAAAAGTACAAACCCGCCGAAGC‑GGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                                             <  5:1188397/150‑1 (MQ=255)
                                                                              gcgactgggttagttCTATCTGGCCCTCAAAAAGAACAAACCCGCCGAAGC‑GGGTTATTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                                             <  6:965404/137‑1 (MQ=11)
                                                                              gcgACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                                             <  5:859203/149‑1 (MQ=25)
                                                                                 aCAGTCTTTTTTCTTTCTGGCACTCCACAATTATAAACCCGCCGTATC‑GGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAc                                                          <  7:744022/151‑1 (MQ=11)
                                                                                   agtgtctgatatttCTGCCACTCAAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                                                        <  6:269881/140‑1 (MQ=11)
                                                                                    gTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGATTTTACGTAAAACATATGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTAGTT‑GAAATTAAATCATCTTTCTGCCCCGCCAAAAGCAAAAACccg                                                       >  8:1450657/1‑151 (MQ=11)
                                                                                       attcatCTTTCTGCCCCTCCAAAAGAAAAAAAACGCAAAAGC‑GTCTTTTTACGTAAAACAGTTGAAACTAACCTATAAGACGCTTTCTTAAGGGTATATAAAAAAA‑AACAATCATTGATCTTTCTGCCCCTCCAAAAGCaaaaaaaaggc                                                     >  1:683887/1‑144 (MQ=2)
                                                                                        ttcttatttctcgcacTCCAAAATTAAAAACCCGCCGAATC‑GGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                    <  4:1195017/135‑1 (MQ=11)
                                                                                          tttatttatggaacTCCAAAAGTTTAAACCGGCCGTATA‑GGTTTTTTACGTAAATCAGGTTAAACTGACAGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTG‑TACAGTCTTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                  <  4:160983/137‑1 (MQ=11)
                                                                                          catCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACATCGGAAGATCACAGATATGACCGGGTCTGTGGGTGATAGAGTCGTG‑GTGACGGATTCTGCTTTCATCAACACCAAAAGCAAAAACccgccggcg                                                 >  6:1463810/1‑148 (MQ=2)
                                                                                           gtCTTTCTGGCCCTCCACAAGATCAAACCCGCCGTAGC‑GGTTTTTTACGTACATGAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                 <  6:195566/149‑1 (MQ=11)
                                                                                            actatctcgcacTCCACAAGAACAAACCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                <  3:1401403/139‑1 (MQ=11)
                                                                                                 agcgcccTCAAAAAGTTAAAACCCGCCTAATC‑GGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                          <  3:886467/147‑1 (MQ=11)
                                                                                                   gCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAATCAGGTGAAAATGTCCGATGAGCAGCGTTCTTATGTGTATTGAGTGGGG‑GAAAGGGCTTAATCGGTCTTCGGCTTCAAAAAAAAAAACCCGGCGCGGCgggggttt                                        >  3:1543963/1‑146 (MQ=2)
                                                                                                   gCCCCTCCAAAAGCACAAACCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                          <  2:372564/149‑1 (MQ=9)
                                                                                                   gCCCCTCCAAAAGCACAAACCCGCCGAAGC‑GGTTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                          <  3:395493/149‑1 (MQ=9)
                                                                                                   cccacTCAAAAAGCACAAACCCGCCGCACC‑GGGTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                         <  5:1333121/146‑1 (MQ=11)
                                                                                                    ccccTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGTGTGGTG‑GCGAGTCAGTCATGTTTGCGCCGGGACAACAGCAAAAACCCGCCGAAGgggggggttt                                       >  5:371462/1‑145 (MQ=11)
                                                                                                      ccTCCAAAAGCAAAAACCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                     <  5:1032313/151‑1 (MQ=14)
                                                                                                         ccAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                            >  1:1022197/1‑141 (MQ=35)
                                                                                                         caaaaaGCAGAAACCCGCCGTAGC‑GGGTTTTTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                    <  4:370300/147‑1 (MQ=11)
                                                                                                             aatcttaaaCCCGCCGTATC‑GGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                              <  1:1323684/145‑1 (MQ=11)
                                                                                                             aatctcaaaCCCGCCGTACC‑GGTTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                              <  7:1442877/145‑1 (MQ=11)
                                                                                                             aaggagaaaCCCGCCGTACC‑GGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                              <  6:1163283/145‑1 (MQ=255)
                                                                                                             aagcacaaaCCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                              <  2:679044/145‑1 (MQ=255)
                                                                                                              agctcaaaCCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                             <  1:945970/146‑1 (MQ=255)
                                                                                                                  aaaaCCCGCCGCAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                         <  6:1130961/151‑1 (MQ=25)
                                                                                                                  aaaaCCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                         <  3:1114643/151‑1 (MQ=35)
                                                                                                                    aaCCCGCCGAATC‑GGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                       <  1:395635/151‑1 (MQ=25)
                                                                                                                     aCCCGCCGAATC‑GGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                      <  4:1093247/151‑1 (MQ=255)
                                                                                                                      cccGCCGTATC‑GGTTTTTTAAGTAAAACAGGTTAAACTTACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                     <  4:551845/151‑1 (MQ=11)
                                                                                                                      cccGCCGTACC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                     <  6:54576/151‑1 (MQ=25)
                                                                                                                          ccgtatc‑GGTTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                 <  7:1448700/145‑1 (MQ=255)
                                                                                                                          ccgcatc‑GGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                 <  7:1281516/145‑1 (MQ=255)
                                                                                                                          ccgcacc‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                 <  2:574110/145‑1 (MQ=25)
                                                                                                                           ccgaatcttttttttACTTAAATCAGGTTAAACTTACTGATAATCTGTTTTCTTTTGTTTTTTGTGTCGTT‑GACATTTATTCATCTTTCTGCCACTCAAAAATCAAAAACCCGCCGAATCTTTTTTTTACGTAAACCAGGTGAAACTGAcc                 <  7:514676/141‑1 (MQ=255)
                                                                                                                              aagtttttttttACTTAAATCTGTTGATATTTACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGa               <  1:580483/143‑1 (MQ=11)
                                                                                                                                           attAACACACGTAAAACTTACCGATATTCCGAATTGTTCTGGTTATAG‑GGGGTGTGATATTCATTCCTCTATATGCCCCTCAAAAATCACAAACCCGCCGCACCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct  <  5:809141/148‑1 (MQ=2)
                                                                                                                                                       |                                                                                                                                          
TGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGC‑GGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTG‑GACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCT  >  NC_000913/3269844‑3270131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑