Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1670CG25.0% 311.9 / 41.9 213intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base C (81/48);  new base G (41/2);  total (156/57)
Fisher's exact test for biased strand distribution p-value = 1.27e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

ATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270026‑3270317
                                                                                                                                              |                                                                                                                                                       
atagcggcctGGACACTCATTCATCTTTCTTTTAATCAAAAAGCAAAAACCCTCATATTCTGTTTTTTATTTAATCTTTGTGAAAATGACGGTTTTGCGTTTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTcc                                                                                                                                                <  7:517793/142‑1 (MQ=2)
           gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCCGGCCTTA‑AAGGTTGTGCCCCGGCAAAAACaaaa                                                                                                                                      >  5:79712/1‑150 (MQ=11)
             cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATC‑AAGTTTCAGCCCCAACAAAAACAAAAAcc                                                                                                                                   >  2:262259/1‑151 (MQ=11)
               gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTAGGGTATAGCGATGTGGACAGTCATGCAAAGTTTGTCCCCATCAAAACCCAAAAAccc                                                                                                                                  >  3:1418205/1‑151 (MQ=11)
                tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGAGTCTTGTAAAGTCAATC‑AAAATTTAGCACCAACAAAAACAAAAACccgc                                                                                                                                >  1:541722/1‑151 (MQ=11)
                  attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGTCGAGGCAATC‑ATGGTTGGCCGCCAACAAAACGAAAAACccgccg                                                                                                                              >  3:246376/1‑151 (MQ=11)
                  attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGCCTCGATGAAAGGCAATC‑ATAATAACGCGGCTGCAAAACCAAAAACccgccc                                                                                                                              >  6:449917/1‑150 (MQ=11)
                      cccccgctcccccTCCACACGCACAACCCCGCCGAAGCGGGTTTTTATTTAAAACAGCTCATCCTCACCGATATTTCTCTTTTTTTTCCTTATATCTTCGCGTACAGTCATTG‑ATCTTTATGTCCCTCCAAAAGAAAAAACCCGCCGAAG‑c                                                                                                                         <  7:813880/142‑1 (MQ=2)
                        cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCCTTG‑ATCGTTGTGCGCCGGCAAAAACAAAAACCCGCCGCAG‑Cgg                                                                                                                       >  6:256313/1‑151 (MQ=11)
                        atatcgcccacTCCACAAGCTTAAACCCGCCGAAGCGGGTTATTACGTAAATCAGGTCATACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑Cgg                                                                                                                       <  5:516713/141‑1 (MQ=11)
                         ccccccccccccccAAACCAAACACCCGCCCACGCGGGTTCTTACGTAAATCTTGTGAACCTGACCCCGCAGCCGGTTTCTTCTGCTTCTCCCGGGCTGGAACCCCATTC‑ATCTTCCTCCCCCTCCAAAAGCAAAAACCCGCCGAAG‑Cggg                                                                                                                      <  6:1468689/145‑1 (MQ=2)
                            cTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGCCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCCTTG‑ATCGGTGTGGCCCGCCAAAAGCAAAAACCCGCCGCAG‑Cgggggg                                                                                                                   >  5:150584/1‑148 (MQ=11)
                              ggcacTCAAAAATCACAAACCCGCCGTATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGttttt                                                                                                                 <  1:207528/147‑1 (MQ=11)
                              gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGAGAGTCAATC‑ATGGGTAGGCACCTGCAAAACCAAAAACCCGCCGCAG‑ACGGtgttt                                                                                                                 >  2:1556382/1‑151 (MQ=11)
                               ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAATC‑AAAATTAAACCCCAACAAAAACAAAAACCCGCCGAAG‑CAGGttatt                                                                                                                 >  4:538707/1‑150 (MQ=11)
                                 ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCCTCGTGGAAAGGGCTTG‑ATGGTGATCCGCCCCCAACAACAAAAACCCCGCTTAG‑GGGGTTTTTAcc                                                                                                              >  3:387984/1‑150 (MQ=2)
                                   tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATGTTTCAGGCCCTCCAAAAGCAAAAACCCCCCGAAG‑AGGGGTTTTAAGtt                                                                                                            >  1:267097/1‑150 (MQ=11)
                                    ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGACGTGGCCAGTCATTC‑ATCTTTGTGCCCCGCCAAAAGCAAAAACCCGCCGCAG‑CGGGTTTTTACGTa                                                                                                            >  3:1270217/1‑150 (MQ=255)
                                     cAAAAGCAAAAACCCGCCTAATTTTTTTTTTAATTTTACAAGTTGTATATGAACTATAATCCGTTTTCTTTTTGGTTTATCGTCGTTGATAGGATTTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTaaa                                                                                                          <  7:1470354/151‑1 (MQ=2)
                                       aacgCACAAACCCGCCGCACCGGTTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATc                                                                                                        <  6:1597436/148‑1 (MQ=11)
                                       aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGCAAGGCATTC‑ATGTTTATCCCCCGACAAAAACAAAAACCCGCGGCAG‑GAGGTGTTTACGGAAATc                                                                                                        >  7:894920/1‑151 (MQ=11)
                                        aagctaatccccGCCGAAGCGGGTTTTTACTTATAACAGTTGCATCTTACGGTTATGGCGCTTTTTTTTGGGTTTTTCGTTGTGGACACTCATTT‑ATTTTTCTGGCCCTGCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCa                                                                                                       <  4:1360913/142‑1 (MQ=2)
                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGTCATTC‑ATATTTGTGCAGCTACAAAAACAAAAACCCGCCGAAG‑AGCGTTTTTAAGTAAATCAGGTg                                                                                                   >  7:33971/1‑151 (MQ=11)
                                             aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATC‑ATAATAGTGCCCCAACAAAAACAAAAACCCGCCTAAG‑ACGGTTTTTAACAAAATCAGGTg                                                                                                   >  4:461892/1‑150 (MQ=11)
                                              aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATATTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGa                                                                                                  >  2:931694/1‑150 (MQ=255)
                                              aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATGGTTCTGCCCCGGCAAAAGCAAAAACCCGCCGAAG‑CGGGTGTTTACGGAAATCAGGTGa                                                                                                  >  6:176573/1‑150 (MQ=11)
                                              aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATATTTATGCACCACCAAAACCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCACGTGa                                                                                                  >  1:1309677/1‑150 (MQ=11)
                                               aaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATATATAAGCACCAACAAAAACAAAAAACCGCCGAAG‑AGGGTAGTTAAGTAAATCACGAGaa                                                                                                 >  1:1133132/1‑150 (MQ=11)
                                                aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTATAGCGTCGTGGACAGGCATTC‑AAATTGGAGCCCCGACAAAACCAAAAACCCGCAGCAGCCCGGTGTTTACGTaatcacgggaaa                                                                                                 >  8:1198218/1‑140 (MQ=2)
                                                 cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGTACAGTCATTC‑ATATTTAAGCCCCAACAAAAACAAAAACCCGCATAAG‑AGCGGTTTTAAGTAAATGACGTGaaaat                                                                                              >  8:556808/1‑149 (MQ=11)
                                                  ccgccgAAGCGGGTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCACGCATAG‑ATGATTGTTCACCTCCAAAAGCAAAAAAACGCCGAAG‑CGGGTTTTGACGTTAATAAgggcacactt                                                                                             >  5:838322/1‑143 (MQ=11)
                                                      cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTC‑ATGGTTGGGCGCCGACAAAAGCAAAAACCCGGCGCAG‑CCGGTGTTTACGGAAATCAGGGGAAACTGACCg                                                                                         >  5:531923/1‑151 (MQ=11)
                                                       gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATC‑ATCTTTATGCACCGACAAAAACAAAAACCCGCCGCAG‑AGGGGGTTTAAGTAAATCAGGTGAAAATTACCg                                                                                         >  8:1380637/1‑150 (MQ=11)
                                                       gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTCGGTATAGCGTCGTGGACAGTCATTC‑AAAATTGATCACAAACAAAAACAAAAACCCGCCGAAG‑CAGCTTAATAAGAAAATCAAGAGAAACTTAccc                                                                                         >  7:2320/1‑149 (MQ=2)
                                                        aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATGTTTGGGCCCCTGCAAAACCAAAAACCCGCCGCAG‑CGCGGTTTTAAGTATATCAGCTGAAAATTACCCAt                                                                                       >  3:305161/1‑151 (MQ=11)
                                                        aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGCATTC‑ATAATTGAACACCAACAAAAACAAAAACCCGCAGAAG‑ACGGGTTTTAAGGAAATCACGTGAAAATTACCGAt                                                                                       >  4:292924/1‑151 (MQ=2)
                                                         cgCGGGTTTTTACGTAAACCACGTCAAACTGACCTTTTTTTTTTTTTGTTTTGGGTATATCGTAGTTGTAATTTATTG‑ATTATTCTGTTTCTCTAAAAGAATATATTCGGATATG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATa                                                                                      <  1:1457412/150‑1 (MQ=255)
                                                             ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTC‑ATCTTTGTGCGCCGGCAAAAGCAAAAACCCGCCGCAG‑CGGGTTTTTACGTAAATCAGGGGAAACTGCCCGATAAGcc                                                                                  >  3:1195803/1‑151 (MQ=11)
                                                                      ttattataGGTGAAACTGAGAGATATACAGTTTTGTTTTGTGTATTTCGTTTTTGTAAGCTATTC‑AGATCTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTg                                                                          <  1:391384/143‑1 (MQ=2)
                                                                        aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGAAGTGCTTG‑ATGTTTGTGCCCCAGCAAAAGCAAAAACCCCCCGCAG‑GCGGTTTTTACCTTAATCAGGTGCAACAGGCCGATAAGCCGGGTTgtgtgg                                                                       >  5:1156128/1‑149 (MQ=9)
                                                                         aaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCCATG‑ATGTTTTGGCGCCGGCAAAAACAAAAACCCGCCGCAG‑GGGGGTTTTAAGGTAATCACGTGAAAATGACCCATAAGCCGGGTTgtgtggg                                                                      >  5:683427/1‑148 (MQ=9)
                                                                         aaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTGATTG‑ATCTTTCGGCCCATCCACAACCAAAAACCCCCCGAAG‑CGGGTTTTTAGGGAAATAAGGTGAAAATGACAGCTAAGCCGGGGTATTTCGt                                                                      >  3:784364/1‑151 (MQ=11)
                                                                             aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCATTGAGCGCCTCCAAAACCAAAAACCCGCAGAAG‑ACGGGGTTTACGTAAATCACGTGAAAATTACCGATAATCCGGGTTCTGTCGAggg                                                                   >  4:1386214/1‑149 (MQ=14)
                                                                             aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATA‑ATAATTATGCCCCGCCAAAAACAAAAACCCCCCGAAG‑CGCGGTTTTAAGGAAATGAGGTGAAAATGACCCATAAGCCGGGTTCTGTCGGGGAc                                                                  >  5:1002540/1‑151 (MQ=14)
                                                                             aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGTAAAGTAAATC‑AAATTTAAGCCCCAACAAAAACAAAAACCCGCAGAAGACGGGTATTAAC‑TAAATCAACAGAAAAATACCGATAAGACGGGTTcactagacga                                                                   >  7:61189/1‑141 (MQ=2)
                                                                             aGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTCGTGTCAAGTCAATC‑AACATAAAAAAACAACAAAAGAAAAAACAAGCAAAAG‑AGGGTTTATTAGAAAATCACGAGAAAGATACCGATTAGCCGGGTGATGTAGTggg                                                                   >  7:166459/1‑149 (MQ=2)
                                                                              ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATATTTTATTTGAAAGTGATTC‑ATCTTTCTGCCACTCTAAAAGCAAAAATCTTCCGAAG‑CTGGTTTTTATTTATATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                                                 <  1:180344/151‑1 (MQ=14)
                                                                               gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTA‑ATCTTTGTGCCCCAACAAAACCAAAAACCCGCCGAAG‑CGGGGTTTTACCTAAATAAGGTGAAAATGCCCGAAAAGCCGGGGTCTGtggagggc                                                                  >  1:527672/1‑142 (MQ=11)
                                                                                  aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCACTCAATC‑ATCTTTAAGCCCCAGCAAAAGCAAAAACCCGCCGACG‑CCGGGTTTTACCTAAAACACGTGAAACTGACCGATAAGCCGGGTTATGTctgggagacac                                                              >  1:1525106/1‑140 (MQ=11)
                                                                                  aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAACAAAAACCCGCCGAAG‑CCGGTGTTTAAGTAAATCAGGTGAAAATGACCCAGAAGCCTGGTTCTGTCGTGGACAGtc                                                              >  4:772397/1‑150 (MQ=17)
                                                                                   aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCAACAAAAACAAAAAACCGCCGCAG‑CCCGTTTTTACGTAAAACAGGTGAAACTTCCCCATAAGCCGGGTTCTGTAGTggcaagacat                                                            >  2:316125/1‑143 (MQ=14)
                                                                                    actcttcctatatccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcatt                                                           <  1:291482/138‑1 (MQ=255)
                                                                                        aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTA‑ATCTTTCTGCCCCTCCAAAAACAAAAACCCCCCCAAG‑CGGGTTTTTAAGTAAATAAAGTGAAACTGACCGATAAGCCGGGTTATTTAGTGGACAgagatagga                                                        >  7:781665/1‑142 (MQ=14)
                                                                                          cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCt                                                      >  3:69805/1‑150 (MQ=255)
                                                                                          cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTA‑ATCTTTAAGCCCCACCAAAAACAAAAAAACCCCCAAG‑CCCGGTTTTACGTAAATCAGGAGAAAAAGAACGAGAAACCGGGGGCTGTCGTGGTCAGGCATTAATCt                                                      >  7:1290660/1‑150 (MQ=9)
                                                                                            aTAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                                                      <  2:1052349/132‑1 (MQ=255)
                                                                                             ttagcagcTTTCTTTTGGGTATAGAGTCTTGGACAGTCATTC‑ATCTTTCTGCCTCTCCAAAACGAAAAAGCCGGCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAgg                                                   <  1:906933/148‑1 (MQ=17)
                                                                                               aGCCGCTTTCTTTTTTGTATTTTTTTGTGTACAGTGATTC‑ATCTTTATGCCAATACAATAGCAAAAACCGTCTGAAT‑AGTGTTTTTTCTTAATTTCTGTTAAACTGACCGATAAGCCGTGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                                                <  8:621761/151‑1 (MQ=255)
                                                                                                  cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                                              >  6:957315/1‑150 (MQ=255)
                                                                                                      ttctttTGGGTATAGTGTTTTTTAAATTAATTC‑ATCTTTCTGCACATACAAAATAAAAAACCCTCTGAAG‑CGGTTTTTTACGTAAATCAGTTGAATCTGATCGATAATGGGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                                          <  1:742320/150‑1 (MQ=12)
                                                                                                      tccgcacgcggagaGCGGCGCGCACAGGCATTC‑ATCTTTTTGTCCCTCACAAATGTCAAACCCCCCTATG‑TGATTTTTTAAGTACATCAGCCGTATGTTCCTGATATGCGGGGTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                                           <  2:961860/136‑1 (MQ=255)
                                                                                                       tctttTGGGTATAGCGTCGTGGTCAGTGAATC‑ATCTTTCTGCCCCGCCAAAAGCAAAAAACCCCCGCAG‑CGGGGTTTTAACTTAATCACGTGTAACTTACCCATAAGCCGGGTTATGTCGTTGTCAGGCAATAATCAAGGCCCGCAATCGc                                        >  2:1278113/1‑151 (MQ=255)
                                                                                                       tctttTGGCTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTACCCCTCCAAAAGCAACAACCCGCCGAAG‑CGGGTTTTTACTTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg                                         <  6:517581/150‑1 (MQ=255)
                                                                                                        ctttTGGGTATAGCGTCGTGGAGAGTCATTG‑TTCGTTAGTCCCCTCCAAAAGCAAAAAACCGCCCAAT‑CGGGGGTTTTAGTTTAAAATGTGAAAAAGACCGAAAAACCGGGGTGGGTCGGGGGCAGTGATTCATCAAGGCCACCaaacccg                                       >  6:1310921/1‑146 (MQ=255)
                                                                                                         ttttGGGTATAGCGTCGTGGACAGTAATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc                                      >  4:1007844/1‑151 (MQ=255)
                                                                                                             gggTATAGCGTCGTGGAGAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCTAAAACCCGCCGATG‑CGGGTTTTTTCGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACt                                   <  2:536662/150‑1 (MQ=255)
                                                                                                              ggTATAGCGTCGTGGAGATTCAGTC‑ATCTTTCAGCGCATCCAATAGCTATAACCCGGAGACG‑CGGGCTTTTACGTAAAGAACGTGAATCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg                                 <  2:1186399/151‑1 (MQ=255)
                                                                                                                      gtcgtGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          >  3:805200/1‑150 (MQ=255)
                                                                                                                      gtcgtGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          >  6:1089746/1‑150 (MQ=255)
                                                                                                                       tcgtGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          >  6:754353/1‑149 (MQ=255)
                                                                                                                        ggtgtACAGATATTG‑ATCTTTGTGCCGCTCCAACACGATATCTCCTCCGAAG‑CTGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcagc                       <  3:630291/150‑1 (MQ=25)
                                                                                                                        gcttgagatttattc‑atGTTTATGCCCCTCCAAAAGAAAAAACCCGGGGAAG‑CGGGTTTTTTCTTAAATCAGGTGAAAGTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATGTAGGCCAGCAATCGCTCACTGGCTCAagcagc                       <  6:1519026/140‑1 (MQ=255)
                                                                                                                        cgtGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcagc                       >  2:1614575/1‑151 (MQ=255)
                                                                                                                         gtGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                      <  2:1463234/151‑1 (MQ=255)
                                                                                                                         gtGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                      >  3:330453/1‑151 (MQ=255)
                                                                                                                         gtGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTTTGGTGGATAGTCTGTACTCTAGGCCTGCAATTTCGCACAGGCTCAAGCAGcc                      >  2:132245/1‑151 (MQ=14)
                                                                                                                            gACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc                   >  2:755832/1‑151 (MQ=255)
                                                                                                                            gACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATATAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc                   >  3:1539892/1‑151 (MQ=255)
                                                                                                                             aCAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc                  >  3:126147/1‑151 (MQ=255)
                                                                                                                               atttattc‑atCTTTCTTCCCCTACAAAATCAAAAACCATCCGAAT‑TGGGTTTTTACGTAAATCAGGTTTAACTGATTGATAAGGCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAccc                 <  8:1612423/146‑1 (MQ=255)
                                                                                                                               aGTCATTC‑ATCTTTCTGCCCCTCCAATAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAATTCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGTCAGTCATTCATCTAGGCCAGCAATCGCTCAATGGCTCAAGCAGCCTACCCg                >  8:227334/1‑151 (MQ=255)
                                                                                                                               aGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg                >  5:1384683/1‑151 (MQ=255)
                                                                                                                                ttaattc‑atCTTTCTTACACTCAAAAATAAAAAATTAGCCTAAG‑CGTTTTTTTACTTAAATCAGGTTAAACTGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg                <  7:1445385/147‑1 (MQ=255)
                                                                                                                                gccactc‑atCTTTCTGCCCCTCCAACACCAACACCGCGCAGTAG‑CGGTTTTTTAGTTAAATCAGGTGACACTGACCGATAAGCCTTGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg               <  5:208336/146‑1 (MQ=25)
                                                                                                                                gTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg               >  6:120220/1‑151 (MQ=255)
                                                                                                                                gTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg               >  3:53924/1‑151 (MQ=255)
                                                                                                                                 tcattc‑atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg              >  2:601749/1‑151 (MQ=255)
                                                                                                                                  cattc‑atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt             >  5:681469/1‑151 (MQ=255)
                                                                                                                                   attc‑atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGtt            >  7:443082/1‑151 (MQ=255)
                                                                                                                                   attc‑atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGtt            >  5:614176/1‑151 (MQ=255)
                                                                                                                                     tc‑atcaTTCTGGCCCTCAAACAGCAAATACCCGACGATG‑TTCGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           <  1:1384428/150‑1 (MQ=37)
                                                                                                                                     tc‑atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGGGGAGAGTCTCTGGTCGAGTCCATCAATCTCTGCCTGGCTCAAGCAGAATACCCcggggc           >  3:1079262/1‑147 (MQ=2)
                                                                                                                                     tc‑atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           >  3:283477/1‑150 (MQ=255)
                                                                                                                                      tcctatttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           <  4:741125/145‑1 (MQ=255)
                                                                                                                                      c‑atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGACAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCat         >  2:1419688/1‑150 (MQ=255)
                                                                                                                                        atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGt        >  4:721595/1‑151 (MQ=255)
                                                                                                                                          cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTa       >  7:1223367/1‑150 (MQ=255)
                                                                                                                                           tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     >  1:450744/1‑151 (MQ=255)
                                                                                                                                            ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGAc                                                                  <  6:600434/89‑1 (MQ=255)
                                                                                                                                             tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACgg    <  6:833912/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                       
ATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTC‑ATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270026‑3270317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑