Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,368,214 C→T Q130* (CAG→TAG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,2140CT99.6% 1738.1 / ‑3.1 462Q130* (CAG→TAG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (247/213);  total (249/213)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 462 total aligned reads displayed.

GGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCG  >  NC_000913/3368067‑3368360
                                                                                                                                                   |                                                                                                                                                  
gggTTCTCTGCAAAAAACTTACGCCAAATGATGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCATAATTCCCATCGGCAAAGATTTGGTAg                                                                                                                                                  <  6:169750/150‑1 (MQ=255)
 ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGAc                                                                                                                                                <  7:320625/151‑1 (MQ=255)
 ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGAc                                                                                                                                                <  2:493588/151‑1 (MQ=255)
  gTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACg                                                                                                                                               <  4:1241967/151‑1 (MQ=255)
      tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGt                                                                                                                                           >  4:1368936/1‑151 (MQ=255)
           aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGcc                                                                                                                                      >  8:82247/1‑151 (MQ=255)
            aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCa                                                                                                                                     <  8:711557/151‑1 (MQ=255)
              aaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCaa                                                                                                                                    >  8:443648/1‑150 (MQ=255)
               aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAAt                                                                                                                                  >  6:1447232/1‑151 (MQ=255)
               aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAAt                                                                                                                                  <  2:102447/151‑1 (MQ=255)
                   tACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCt                                                                                                                                <  4:41066/149‑1 (MQ=255)
                    aCGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTc                                                                                                                             <  1:828836/151‑1 (MQ=255)
                       ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTcccc                                                                                                                          <  2:1552830/151‑1 (MQ=255)
                        cAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCt                                                                                                                         >  7:59951/1‑151 (MQ=255)
                         aaaTGAGGCTTTTTTACCTCTTTTTTCAACATCTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCtt                                                                                                                        <  6:282296/151‑1 (MQ=255)
                          aaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTg                                                                                                                       >  5:264226/1‑151 (MQ=255)
                          aaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTg                                                                                                                       >  3:97270/1‑151 (MQ=255)
                           aTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGc                                                                                                                      >  7:528471/1‑151 (MQ=255)
                             gAGGCTTTTTTACCTCTTTTTTAAACATGTTGAAATTCACCAGACAATGTCTGACGAATTAACACCATTGGGAATTCAGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGTAGACTCTGTCTGCCAAATCTTTCCCCTTGacc                                                                                                                    >  7:1501504/1‑151 (MQ=255)
                              aGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCa                                                                                                                   <  2:443356/151‑1 (MQ=255)
                                gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATAGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAgg                                                                                                                 >  3:901802/1‑151 (MQ=255)
                                    ttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTccc                                                                                                             >  3:1525061/1‑150 (MQ=255)
                                    ttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTcc                                                                                                              >  5:161377/1‑150 (MQ=255)
                                     tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCa                                                                                                             <  8:253718/150‑1 (MQ=255)
                                     tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                                                            >  8:1582823/1‑151 (MQ=255)
                                     tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                                                            >  5:1462830/1‑151 (MQ=255)
                                     tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                                                            >  8:1394636/1‑151 (MQ=255)
                                          ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                       >  3:496668/1‑151 (MQ=255)
                                          ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                       >  5:715074/1‑151 (MQ=255)
                                            ctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTc                                                                                                     >  2:728464/1‑151 (MQ=255)
                                              tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTccc                                                                                                   >  7:1016433/1‑150 (MQ=255)
                                              tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTcc                                                                                                    <  3:233707/150‑1 (MQ=255)
                                              tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCg                                                                                                   >  6:1328891/1‑151 (MQ=255)
                                                 ttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                                                                >  5:839894/1‑151 (MQ=255)
                                                  tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                                                                <  8:1458220/150‑1 (MQ=255)
                                                  tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                                                                >  3:657522/1‑150 (MQ=255)
                                                   cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAc                                                                                              >  2:1586797/1‑151 (MQ=255)
                                                    aaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTcc                                                                                                              >  4:299367/1‑134 (MQ=255)
                                                    aaCATGTTGAAATTCACCAGACAATGTATGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACt                                                                                             <  3:554372/151‑1 (MQ=255)
                                                     aCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACtt                                                                                            >  1:955093/1‑151 (MQ=255)
                                                             aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                                                     >  1:301761/1‑150 (MQ=255)
                                                             aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTACCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                                                     >  2:961197/1‑150 (MQ=255)
                                                               aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCCCCTACGTCCGTTTACTTTCCGTTaaa                                                                                   >  6:1197138/1‑150 (MQ=255)
                                                                  cACCAGACAATGTCTGGCGAATTAAAACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaaaac                                                                               >  3:1219947/1‑149 (MQ=255)
                                                                   aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                              <  5:215740/151‑1 (MQ=255)
                                                                   aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                              <  1:1171000/151‑1 (MQ=255)
                                                                    ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                                                             <  5:1135249/151‑1 (MQ=255)
                                                                    ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                                                             >  2:1270701/1‑151 (MQ=255)
                                                                    ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                                                             <  8:927475/151‑1 (MQ=255)
                                                                    ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCAGCATACACCGTCTGCAGAAATGACATCCGCAAAGATTTGGTAGACGCTCTCTGCCAAATCTTTACCCTTGCCCAGGTCAACATACGAACGATTAAATACCGTTAAAAActc                                                                              >  7:698279/1‑148 (MQ=255)
                                                                      aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                            >  1:256229/1‑150 (MQ=255)
                                                                      aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGGATGCAGAATTCCCATCGGCAAAGATTTGGTAGACTCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                                                           <  1:419928/151‑1 (MQ=255)
                                                                       gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                                                                          <  6:885244/151‑1 (MQ=255)
                                                                           aTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                                                      <  4:1596095/151‑1 (MQ=255)
                                                                           aTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                                                      >  3:1321845/1‑151 (MQ=255)
                                                                           aTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAACGCTGACGccc                                                                      >  1:617477/1‑151 (MQ=255)
                                                                             gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCg                                                                     <  2:292446/150‑1 (MQ=255)
                                                                             gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCg                                                                     <  1:1272380/150‑1 (MQ=255)
                                                                              tCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCCCCTACGTCCGTTTACTTTCCGTTAAACACGCTGACGCCCGcc                                                                   >  1:1009658/1‑150 (MQ=255)
                                                                                tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCa                                                                                                             >  6:1534558/1‑107 (MQ=255)
                                                                                  gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCtt                                                               <  5:942393/151‑1 (MQ=255)
                                                                                   cGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGCCGCCCGCAGCttt                                                              >  1:720192/1‑151 (MQ=255)
                                                                                     aaTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCttttt                                                            >  8:1278685/1‑150 (MQ=255)
                                                                                     aaTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGAAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTACGTTAAAAACGCTGACGCCCGCAGCtttt                                                             >  3:31648/1‑150 (MQ=255)
                                                                                          acacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaa                                                        >  2:372633/1‑150 (MQ=255)
                                                                                           cacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaa                                                      <  3:275794/151‑1 (MQ=255)
                                                                                             ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag                                                    <  2:651428/151‑1 (MQ=255)
                                                                                                ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag                                                    >  7:1189593/1‑148 (MQ=255)
                                                                                                 tGGGAATTCCGCAGACACCGGCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAgaga                                                 <  5:622772/150‑1 (MQ=255)
                                                                                                  ggtaaTTCCGCACACACCGTCTGCAGAATTCCCATCGTCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCATCTTTTATGAAAAAGAGAc                                                <  8:538923/147‑1 (MQ=255)
                                                                                                  gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGAc                                                <  2:1201842/150‑1 (MQ=255)
                                                                                                   ggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGc                                              <  8:782548/151‑1 (MQ=255)
                                                                                                    gAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCt                                             >  3:1380426/1‑151 (MQ=255)
                                                                                                        tCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGc                                         >  7:412190/1‑151 (MQ=255)
                                                                                                         ccGCAGACACCTTCTGAATAATTCCAATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACATACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                                        <  2:906055/151‑1 (MQ=255)
                                                                                                         ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                                        >  4:782763/1‑151 (MQ=255)
                                                                                                         ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                                        >  8:1148920/1‑151 (MQ=255)
                                                                                                         ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTATTATGAAAAAGAGACGCTCCGc                                         >  7:1220588/1‑150 (MQ=255)
                                                                                                          cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg                                       >  1:1604558/1‑151 (MQ=255)
                                                                                                           gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgt                                      >  7:227828/1‑151 (MQ=255)
                                                                                                            cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg                                     >  1:203876/1‑151 (MQ=255)
                                                                                                            cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGACCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg                                     >  4:942360/1‑151 (MQ=255)
                                                                                                             aGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                    >  6:520242/1‑151 (MQ=255)
                                                                                                                 acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCTACGTCCGTATACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTgg                                >  7:985041/1‑151 (MQ=255)
                                                                                                                        gCAGAATTCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                          <  1:1398915/150‑1 (MQ=255)
                                                                                                                              ttCCCATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGc                   >  2:1325413/1‑151 (MQ=255)
                                                                                                                                cccATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCt                  <  2:1394221/150‑1 (MQ=255)
                                                                                                                                cccATCGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTa                 <  7:1342672/151‑1 (MQ=255)
                                                                                                                                     cGGCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCg            >  4:478047/1‑151 (MQ=255)
                                                                                                                                      ggCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCgg           >  4:63526/1‑151 (MQ=255)
                                                                                                                                      ggCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCgg           <  4:70921/151‑1 (MQ=255)
                                                                                                                                      ggCAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCgg           >  4:887536/1‑151 (MQ=255)
                                                                                                                                        cAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCa         <  5:506607/151‑1 (MQ=255)
                                                                                                                                        cAAAGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCa         <  5:288028/151‑1 (MQ=255)
                                                                                                                                          aaGATTTTGTAGACGCTGTCTGCCTAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCaaa       <  3:162765/151‑1 (MQ=255)
                                                                                                                                           aGATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAt      <  3:828007/151‑1 (MQ=255)
                                                                                                                                            gATTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt     <  8:352910/151‑1 (MQ=255)
                                                                                                                                             aTTTGGTAGACGCTGTCTGCCAAATCTTTCCCCTTCCACAGGTCCACCTACGTCCGTATACTTTCCGTTAAAAAAGCTGACGCCCCCAGCTTTTATGAAAAACAGACGCTCCGCTGTTGCTGGTATGTTCGCAAGCTAGAGCGGCAAATTg    >  6:1090905/1‑151 (MQ=255)
                                                                                                                                              tttGGTAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGc   >  6:755557/1‑151 (MQ=255)
                                                                                                                                                tGGTAGACGCTGGCTGCCAAATCTTTCCCCTAGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg  <  5:439539/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCG  >  NC_000913/3368067‑3368360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑