Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,307,744 Δ1 bp coding (902/1461 nt) clsA ← cardiolipin synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,307,7430T.97.2% 1264.2 / 29.4 286coding (903/1461 nt)clsAcardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base . (138/139);  total (145/141)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

Only 100 of 291 total aligned reads displayed.

AAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGC  >  NC_000913/1307593‑1307888
                                                                                                                                                      |                                                                                                                                                 
aaGTAAATCATCGCTTGGCACAAAATAGGGCGTGGTCATGATTAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGt                                                                                                                                                   <  3:404651/151‑1 (MQ=255)
    aaaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGtcaa                                                                                                                                                <  4:298748/150‑4 (MQ=255)
    aaaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGtcaa                                                                                                                                                <  3:1328708/150‑4 (MQ=255)
      atcatcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACg                                                                                                                                            >  5:394136/1‑151 (MQ=255)
        catcGGTTGGCACAAAGTAGGGCGTGGTCATGATCAAATAATCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGc                                                                                                                                          <  3:853548/151‑1 (MQ=255)
        catcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGc                                                                                                                                          >  5:1150309/1‑151 (MQ=255)
          tcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCat                                                                                                                                        >  7:511627/1‑151 (MQ=255)
            gCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCata                                                                                                                                       >  5:1232430/1‑150 (MQ=255)
                ggCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATAtt                                                                                                                                  <  8:792614/151‑1 (MQ=255)
                    caAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGAc                                                                                                                               >  4:653947/1‑150 (MQ=255)
                    caAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGAc                                                                                                                               >  2:27493/1‑150 (MQ=255)
                      aaGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACAt                                                                                                                             >  4:707799/1‑150 (MQ=255)
                          aGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCtgg                                                                                                                         <  6:94059/150‑1 (MQ=255)
                           gggCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCtggtg                                                                                                                       >  3:193944/1‑151 (MQ=255)
                             gCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCtggttgt                                                                                                                     <  7:145236/151‑1 (MQ=255)
                              cGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCtggtggtg                                                                                                                    >  7:1189805/1‑151 (MQ=255)
                               gTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCtggtggtgg                                                                                                                   <  5:475668/151‑1 (MQ=255)
                                gggTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTggc                                                                                                                  <  2:228213/150‑1 (MQ=255)
                                gggTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTggc                                                                                                                  <  4:747531/150‑1 (MQ=255)
                                 ggTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTggcg                                                                                                                 >  4:1021787/1‑151 (MQ=255)
                                    cATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACTTCTGGTGGTggcggcc                                                                                                              >  4:1371227/1‑150 (MQ=255)
                                       gATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGa                                                                                                            >  3:58586/1‑150 (MQ=255)
                                       gATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCCGCAGa                                                                                                            >  3:494305/1‑150 (MQ=255)
                                        aTCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGaa                                                                                                           <  4:213180/150‑1 (MQ=255)
                                        aTCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTCGCCTG‑TCAAACGGCATAATATTGACATCTGGTGCTGGCGGCAGAAt                                                                                                          >  4:1162731/1‑151 (MQ=255)
                                           aaaTATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACg                                                                                                       >  1:299043/1‑151 (MQ=255)
                                            aaTATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGGAGAATACGt                                                                                                      >  7:168011/1‑151 (MQ=255)
                                            aaTATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGt                                                                                                      <  2:942836/151‑1 (MQ=255)
                                             atatTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGtt                                                                                                     <  3:1065996/151‑1 (MQ=255)
                                               atTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGTCCTG‑TCAACCGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGtttt                                                                                                   >  2:147361/1‑151 (MQ=255)
                                                 tCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTcc                                                                                                 >  5:298290/1‑151 (MQ=255)
                                                 tCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTcc                                                                                                 >  3:1111592/1‑151 (MQ=255)
                                                        ccGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCa                                                                                          <  3:792469/151‑1 (MQ=255)
                                                        ccGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTGCCGGAAAGCCGGGGCCAGAAGCATTTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATAGGTTTTCCGGGTTCa                                                                                          <  1:1406415/151‑1 (MQ=255)
                                                         cGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCaa                                                                                         <  8:377030/151‑1 (MQ=255)
                                                          gAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAAt                                                                                        <  7:872782/151‑1 (MQ=255)
                                                           aaTAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAAtc                                                                                       >  4:598835/1‑151 (MQ=255)
                                                            aTAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGCCCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTGAAtct                                                                                      >  7:206525/1‑151 (MQ=255)
                                                             tAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAAtctc                                                                                     >  7:437445/1‑151 (MQ=255)
                                                              aaGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTGCGGAAAGCCTTGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTcc                                                                                    <  5:1353023/151‑1 (MQ=255)
                                                                gCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCa                                                                                  <  5:608802/151‑1 (MQ=255)
                                                                 ccGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTccc                                                                                   >  7:1073218/1‑149 (MQ=255)
                                                                 ccGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTccc                                                                                   >  8:754209/1‑149 (MQ=255)
                                                                 ccGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAg                                                                                 <  6:1315805/151‑1 (MQ=255)
                                                                  cGCAGTCAATAATGCCTGGTGAATGACATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGGGGGACCGCGGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGt                                                                                <  3:770736/151‑1 (MQ=255)
                                                                   gCAGTCAATAATGCCTTGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTc                                                                               <  5:72165/151‑1 (MQ=255)
                                                                   gCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACTGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTc                                                                               >  3:285825/1‑151 (MQ=255)
                                                                     aGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  1:1267277/1‑151 (MQ=255)
                                                                      gTCAATAATGCCTGGTGTATGAGATGTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCa                                                                            <  8:1290274/151‑1 (MQ=255)
                                                                      gTCAATAATGCCTGGTGAATGGGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  2:1283080/1‑151 (MQ=255)
                                                                      gTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  7:704258/1‑150 (MQ=255)
                                                                      gTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCAGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  8:562786/1‑151 (MQ=255)
                                                                       tCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  8:1231358/1‑149 (MQ=255)
                                                                       tCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  1:900853/1‑149 (MQ=255)
                                                                        cAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAgg                                                                          >  2:335637/1‑151 (MQ=255)
                                                                         aataatGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGa                                                                         <  6:321976/151‑1 (MQ=255)
                                                                           taatGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAACCGGCATAATATTGAGATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaat                                                                       >  8:837608/1‑151 (MQ=255)
                                                                           taatGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaat                                                                       >  5:144790/1‑151 (MQ=255)
                                                                            aatGCCTGGTGAATGAGATCTTCCGGAAAGCCCGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaat                                                                       >  5:149961/1‑150 (MQ=255)
                                                                                ccTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaataaat                                                                   >  8:454657/1‑150 (MQ=255)
                                                                                   ggTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTTTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATc                                                               <  2:1183397/151‑1 (MQ=255)
                                                                                   ggTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAaataat                                                                <  1:246661/150‑1 (MQ=255)
                                                                                    gTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATcc                                                              <  3:1221774/151‑1 (MQ=255)
                                                                                     tGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATccc                                                             >  4:793054/1‑151 (MQ=255)
                                                                                      gAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATcccc                                                            >  4:85407/1‑151 (MQ=255)
                                                                                       aaTGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCa                                                           <  4:445899/151‑1 (MQ=255)
                                                                                       aaTGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAAGACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATcccc                                                            >  8:17318/1‑150 (MQ=255)
                                                                                        aTGAGATCGTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCAt                                                          >  6:1234664/1‑151 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcg                                                        <  6:1139648/151‑1 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcg                                                        <  3:1434089/151‑1 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcg                                                        >  6:359956/1‑151 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATc                                                         <  2:662492/150‑1 (MQ=255)
                                                                                           agaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGAGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCACATcgc                                                       >  4:528685/1‑151 (MQ=255)
                                                                                             aTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCgg                                                     >  2:447143/1‑151 (MQ=255)
                                                                                               cTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTg                                                   >  7:1317843/1‑151 (MQ=255)
                                                                                                 tCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCGATCTCCCAGTCGCAGGAATAAATAATCCCCATcgcg                                                      <  7:925001/146‑1 (MQ=255)
                                                                                                 tCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcgcg                                                      <  4:547407/146‑1 (MQ=255)
                                                                                                 tCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGt                                                    <  6:158170/148‑1 (MQ=255)
                                                                                                    ggAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGAt                                              >  4:1214923/1‑151 (MQ=255)
                                                                                                      aaaGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGAt                                              >  6:1452992/1‑150 (MQ=255)
                                                                                                      aaaGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAg                                            <  5:1071264/151‑1 (MQ=255)
                                                                                                         gCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGc                                         <  3:653714/151‑1 (MQ=255)
                                                                                                              ggCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTc                                     >  7:1389260/1‑151 (MQ=255)
                                                                                                              ggCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTcc                                    >  5:839936/1‑151 (MQ=255)
                                                                                                                 cAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCAt                                  >  1:1143221/1‑150 (MQ=255)
                                                                                                                  aGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATAc                                <  7:224991/151‑1 (MQ=255)
                                                                                                                  aGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATAc                                >  6:484721/1‑151 (MQ=255)
                                                                                                                  aGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATAc                                <  6:1035592/151‑1 (MQ=255)
                                                                                                                            gtgtgAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAg                      <  8:255953/151‑1 (MQ=255)
                                                                                                                              gtgAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGGGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGAt                    <  5:809099/151‑1 (MQ=255)
                                                                                                                               tgAATGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATc                        >  6:713562/1‑146 (MQ=255)
                                                                                                                                  aTGGTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAAt                >  1:82575/1‑151 (MQ=255)
                                                                                                                                    ggTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTTGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATc               <  2:1403777/150‑1 (MQ=255)
                                                                                                                                    ggTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATACTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATcc              <  4:609184/151‑1 (MQ=255)
                                                                                                                                    ggTGTGACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATc               <  2:85565/150‑1 (MQ=255)
                                                                                                                                       gtgACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATcattg           >  2:1032306/1‑147 (MQ=255)
                                                                                                                                       gtgACCGCTGGCCTG‑TCAAACGGCATAATATTGACAGCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCAt            <  8:1319030/150‑1 (MQ=255)
                                                                                                                                         gACCGCTGGCCTG‑TCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGc         >  8:678717/1‑151 (MQ=255)
                                                                                                                                           ccGCTGGCCTG‑TCAAACGCCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCTGATCAATCACTTGccc       >  1:1346409/1‑151 (MQ=255)
                                                                                                                                              cTGGCATG‑TCAATCGGGATAATATTGACATGTTGTGGTGGGGGCAGAATACGTTTTACTTTTTCAATCTCCCAGTCGCAGGAATATATAATCGCCATGGCGGTGTCGATAGGGCCTTCCTTACGCGCCATGAGTTCAATCCATTGCGCt      <  3:707518/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
AAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGC  >  NC_000913/1307593‑1307888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑