Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,307,744 Δ1 bp coding (902/1461 nt) clsA ← cardiolipin synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,307,7430T.97.2% 1264.2 / 29.4 286coding (903/1461 nt)clsAcardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base . (138/139);  total (145/141)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

Only 100 of 291 total aligned reads displayed.

AAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGC  >  NC_000913/1307593‑1307888
                                                                                                                                                      |                                                                                                                                                 
aaGTAAATCATCGCTTGGCACAAAATAGGGCGTGGTCATGATTAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGt                                                                                                                                                   <  3:404651/151‑1 (MQ=255)
    aaaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGtcaa                                                                                                                                                <  4:298748/150‑4 (MQ=255)
    aaaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGtcaa                                                                                                                                                <  3:1328708/150‑4 (MQ=255)
      atcatcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACg                                                                                                                                            >  5:394136/1‑151 (MQ=255)
        catcGGTTGGCACAAAGTAGGGCGTGGTCATGATCAAATAATCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGc                                                                                                                                          <  3:853548/151‑1 (MQ=255)
        catcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGc                                                                                                                                          >  5:1150309/1‑151 (MQ=255)
          tcGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCat                                                                                                                                        >  7:511627/1‑151 (MQ=255)
            gCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCata                                                                                                                                       >  5:1232430/1‑150 (MQ=255)
                ggCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATAtt                                                                                                                                  <  8:792614/151‑1 (MQ=255)
                    caAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGAc                                                                                                                               >  4:653947/1‑150 (MQ=255)
                    caAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGAc                                                                                                                               >  2:27493/1‑150 (MQ=255)
                      aaGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACAt                                                                                                                             >  4:707799/1‑150 (MQ=255)
                          aGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCtgg                                                                                                                         <  6:94059/150‑1 (MQ=255)
                           gggCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCtggtg                                                                                                                       >  3:193944/1‑151 (MQ=255)
                             gCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCtggttgt                                                                                                                     <  7:145236/151‑1 (MQ=255)
                              cGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCtggtggtg                                                                                                                    >  7:1189805/1‑151 (MQ=255)
                               gTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCtggtggtgg                                                                                                                   <  5:475668/151‑1 (MQ=255)
                                gggTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTggc                                                                                                                  <  2:228213/150‑1 (MQ=255)
                                gggTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTggc                                                                                                                  <  4:747531/150‑1 (MQ=255)
                                 ggTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTggcg                                                                                                                 >  4:1021787/1‑151 (MQ=255)
                                    cATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACTTCTGGTGGTggcggcc                                                                                                              >  4:1371227/1‑150 (MQ=255)
                                       gATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGa                                                                                                            >  3:58586/1‑150 (MQ=255)
                                       gATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCCGCAGa                                                                                                            >  3:494305/1‑150 (MQ=255)
                                        aTCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGaa                                                                                                           <  4:213180/150‑1 (MQ=255)
                                        aTCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTCGCCTGTCAAACGGCATAATATTGACATCTGGTGCTGGCGGCAGAAt                                                                                                          >  4:1162731/1‑151 (MQ=255)
                                           aaaTATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACg                                                                                                       >  1:299043/1‑151 (MQ=255)
                                            aaTATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGGAGAATACGt                                                                                                      >  7:168011/1‑151 (MQ=255)
                                            aaTATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGt                                                                                                      <  2:942836/151‑1 (MQ=255)
                                             atatTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGtt                                                                                                     <  3:1065996/151‑1 (MQ=255)
                                               atTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGTCCTGTCAACCGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGtttt                                                                                                   >  2:147361/1‑151 (MQ=255)
                                                 tCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTcc                                                                                                 >  5:298290/1‑151 (MQ=255)
                                                 tCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTcc                                                                                                 >  3:1111592/1‑151 (MQ=255)
                                                        ccGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCa                                                                                          <  3:792469/151‑1 (MQ=255)
                                                        ccGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTGCCGGAAAGCCGGGGCCAGAAGCATTTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATAGGTTTTCCGGGTTCa                                                                                          <  1:1406415/151‑1 (MQ=255)
                                                         cGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCaa                                                                                         <  8:377030/151‑1 (MQ=255)
                                                          gAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAAt                                                                                        <  7:872782/151‑1 (MQ=255)
                                                           aaTAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAAtc                                                                                       >  4:598835/1‑151 (MQ=255)
                                                            aTAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGCCCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTGAAtct                                                                                      >  7:206525/1‑151 (MQ=255)
                                                             tAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAAtctc                                                                                     >  7:437445/1‑151 (MQ=255)
                                                              aaGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTGCGGAAAGCCTTGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTcc                                                                                    <  5:1353023/151‑1 (MQ=255)
                                                                gCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCa                                                                                  <  5:608802/151‑1 (MQ=255)
                                                                 ccGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTccc                                                                                   >  7:1073218/1‑149 (MQ=255)
                                                                 ccGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTccc                                                                                   >  8:754209/1‑149 (MQ=255)
                                                                 ccGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAg                                                                                 <  6:1315805/151‑1 (MQ=255)
                                                                  cGCAGTCAATAATGCCTGGTGAATGACATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGGGGGACCGCGGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGt                                                                                <  3:770736/151‑1 (MQ=255)
                                                                   gCAGTCAATAATGCCTTGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTc                                                                               <  5:72165/151‑1 (MQ=255)
                                                                   gCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACTGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTc                                                                               >  3:285825/1‑151 (MQ=255)
                                                                     aGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  1:1267277/1‑151 (MQ=255)
                                                                      gTCAATAATGCCTGGTGTATGAGATGTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCa                                                                            <  8:1290274/151‑1 (MQ=255)
                                                                      gTCAATAATGCCTGGTGAATGGGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  2:1283080/1‑151 (MQ=255)
                                                                      gTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  7:704258/1‑150 (MQ=255)
                                                                      gTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCAGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  8:562786/1‑151 (MQ=255)
                                                                       tCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  8:1231358/1‑149 (MQ=255)
                                                                       tCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGc                                                                             >  1:900853/1‑149 (MQ=255)
                                                                        cAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAgg                                                                          >  2:335637/1‑151 (MQ=255)
                                                                         aataatGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGa                                                                         <  6:321976/151‑1 (MQ=255)
                                                                           taatGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAACCGGCATAATATTGAGATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaat                                                                       >  8:837608/1‑151 (MQ=255)
                                                                           taatGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaat                                                                       >  5:144790/1‑151 (MQ=255)
                                                                            aatGCCTGGTGAATGAGATCTTCCGGAAAGCCCGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaat                                                                       >  5:149961/1‑150 (MQ=255)
                                                                                ccTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGaataaat                                                                   >  8:454657/1‑150 (MQ=255)
                                                                                   ggTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTTTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATc                                                               <  2:1183397/151‑1 (MQ=255)
                                                                                   ggTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAaataat                                                                <  1:246661/150‑1 (MQ=255)
                                                                                    gTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATcc                                                              <  3:1221774/151‑1 (MQ=255)
                                                                                     tGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATccc                                                             >  4:793054/1‑151 (MQ=255)
                                                                                      gAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATcccc                                                            >  4:85407/1‑151 (MQ=255)
                                                                                       aaTGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCa                                                           <  4:445899/151‑1 (MQ=255)
                                                                                       aaTGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAAGACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATcccc                                                            >  8:17318/1‑150 (MQ=255)
                                                                                        aTGAGATCGTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCAt                                                          >  6:1234664/1‑151 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcg                                                        <  6:1139648/151‑1 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcg                                                        <  3:1434089/151‑1 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcg                                                        >  6:359956/1‑151 (MQ=255)
                                                                                          gagaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATc                                                         <  2:662492/150‑1 (MQ=255)
                                                                                           agaTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGAGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCACATcgc                                                       >  4:528685/1‑151 (MQ=255)
                                                                                             aTCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCgg                                                     >  2:447143/1‑151 (MQ=255)
                                                                                               cTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTg                                                   >  7:1317843/1‑151 (MQ=255)
                                                                                                 tCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCGATCTCCCAGTCGCAGGAATAAATAATCCCCATcgcg                                                      <  7:925001/146‑1 (MQ=255)
                                                                                                 tCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcgcg                                                      <  4:547407/146‑1 (MQ=255)
                                                                                                 tCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGt                                                    <  6:158170/148‑1 (MQ=255)
                                                                                                    ggAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGAt                                              >  4:1214923/1‑151 (MQ=255)
                                                                                                      aaaGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGAt                                              >  6:1452992/1‑150 (MQ=255)
                                                                                                      aaaGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAg                                            <  5:1071264/151‑1 (MQ=255)
                                                                                                         gCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGc                                         <  3:653714/151‑1 (MQ=255)
                                                                                                              ggCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTc                                     >  7:1389260/1‑151 (MQ=255)
                                                                                                              ggCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTcc                                    >  5:839936/1‑151 (MQ=255)
                                                                                                                 cAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCAt                                  >  1:1143221/1‑150 (MQ=255)
                                                                                                                  aGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATAc                                <  7:224991/151‑1 (MQ=255)
                                                                                                                  aGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATAc                                >  6:484721/1‑151 (MQ=255)
                                                                                                                  aGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATAc                                <  6:1035592/151‑1 (MQ=255)
                                                                                                                            gtgtgAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAg                      <  8:255953/151‑1 (MQ=255)
                                                                                                                              gtgAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGGGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGAt                    <  5:809099/151‑1 (MQ=255)
                                                                                                                               tgAATGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATc                        >  6:713562/1‑146 (MQ=255)
                                                                                                                                  aTGGTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAAt                >  1:82575/1‑151 (MQ=255)
                                                                                                                                    ggTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTTGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATc               <  2:1403777/150‑1 (MQ=255)
                                                                                                                                    ggTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATACTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATcc              <  4:609184/151‑1 (MQ=255)
                                                                                                                                    ggTGTGACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATc               <  2:85565/150‑1 (MQ=255)
                                                                                                                                       gtgACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATcattg           >  2:1032306/1‑147 (MQ=255)
                                                                                                                                       gtgACCGCTGGCCTGTCAAACGGCATAATATTGACAGCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCAt            <  8:1319030/150‑1 (MQ=255)
                                                                                                                                         gACCGCTGGCCTGTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGc         >  8:678717/1‑151 (MQ=255)
                                                                                                                                           ccGCTGGCCTGTCAAACGCCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCTGATCAATCACTTGccc       >  1:1346409/1‑151 (MQ=255)
                                                                                                                                              cTGGCATGTCAATCGGGATAATATTGACATGTTGTGGTGGGGGCAGAATACGTTTTACTTTTTCAATCTCCCAGTCGCAGGAATATATAATCGCCATGGCGGTGTCGATAGGGCCTTCCTTACGCGCCATGAGTTCAATCCATTGCGCt      <  3:707518/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
AAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGC  >  NC_000913/1307593‑1307888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: