Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,247,487 +A intergenic (+59/‑286) exuR → / → yqjA hexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,247,4861.A94.9% 1361.5 / 54.0 370intergenic (+58/‑287)exuR/yqjAhexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base . (10/9);  new base A (167/184);  total (177/193)
Fisher's exact test for biased strand distribution p-value = 8.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

ACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTT  >  NC_000913/3247336‑3247635
                                                                                                                                                       |                                                                                                                                                     
 ccAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGta                                                                                                                                                      <  6:404612/151‑1 (MQ=255)
     cgacgaCTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATaaa                                                                                                                                                  <  1:1037720/151‑1 (MQ=255)
      gacgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaataaa                                                                                                                                                 <  2:51331/151‑6 (MQ=255)
       ccgacTTCGAGTTGAATGCCGACCGCGATCTATTCGCCGAAAACCCGGTGGTGCATCTCGCTACACCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAg                                                                                                                                                 <  6:1340606/150‑1 (MQ=255)
       acgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAgt                                                                                                                                                <  6:1281566/151‑1 (MQ=255)
        cgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAgtg                                                                                                                                               <  7:412836/151‑1 (MQ=255)
           cTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTc                                                                                                                                             >  8:525972/1‑151 (MQ=255)
            ttCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTc                                                                                                                                             >  2:1215795/1‑150 (MQ=255)
               gAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGcc                                                                                                                                         >  8:455439/1‑151 (MQ=255)
               gAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGcc                                                                                                                                         >  1:953672/1‑151 (MQ=255)
               gAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGcc                                                                                                                                         <  5:161449/151‑1 (MQ=255)
                 gTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtg                                                                                                                                       >  4:47512/1‑151 (MQ=255)
                 gTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtg                                                                                                                                       >  3:777819/1‑151 (MQ=255)
                  ttCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtgt                                                                                                                                      >  6:1061277/1‑151 (MQ=255)
                   tCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtgt                                                                                                                                      <  6:65415/150‑1 (MQ=255)
                    cAATGCCGAGCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTgtgt                                                                                                                                    <  4:970412/151‑1 (MQ=255)
                    cAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTgtgt                                                                                                                                    >  2:62592/1‑151 (MQ=255)
                    cAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTgtgt                                                                                                                                    <  2:976856/151‑1 (MQ=255)
                      aTGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTaa                                                                                                                                  <  7:253653/151‑1 (MQ=255)
                        gCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAAt                                                                                                                                <  8:1328521/151‑1 (MQ=255)
                         ccGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATc                                                                                                                               <  1:1336118/151‑1 (MQ=255)
                            aCCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCctc                                                                                                                            <  8:60575/151‑1 (MQ=255)
                             ccGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATc                                                                                                                               >  7:181545/1‑147 (MQ=255)
                               gCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCg                                                                                                                         <  6:501406/151‑1 (MQ=255)
                               gCTATCTATTCGCCGAAAACCCGGTCGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTCTCAGCCTCTGTAAATCCTCTCg                                                                                                                         >  4:548303/1‑151 (MQ=255)
                                ctatctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGc                                                                                                                        >  1:105961/1‑151 (MQ=255)
                                 tatctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGcc                                                                                                                       >  5:1092517/1‑151 (MQ=255)
                                  atctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGcc                                                                                                                       >  5:222882/1‑150 (MQ=255)
                                  atctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGcc                                                                                                                       <  5:1197264/150‑1 (MQ=255)
                                         cGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTccc                                                                                                               <  4:392931/151‑1 (MQ=255)
                                          gCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTcccc                                                                                                              <  4:73633/151‑1 (MQ=255)
                                          gCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTcccc                                                                                                              >  1:660756/1‑151 (MQ=255)
                                            cGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTg                                                                                                            >  5:1356105/1‑151 (MQ=255)
                                               aaaCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCAt                                                                                                         >  4:1393017/1‑151 (MQ=255)
                                                aaCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCAtt                                                                                                        >  4:1262895/1‑151 (MQ=255)
                                                 aCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGTCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTc                                                                                                       <  6:183462/151‑1 (MQ=255)
                                                 aCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTc                                                                                                       <  7:774313/151‑1 (MQ=255)
                                                  cccGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGGAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCGCTCGCCCCCCTCCCCTGCATTc                                                                                                       >  1:888793/1‑150 (MQ=255)
                                                       tggtgCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCaa                                                                                                  >  6:1430622/1‑150 (MQ=255)
                                                            cATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAg                                                                                            <  8:1161816/151‑1 (MQ=255)
                                                             aTCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATc                                                                                              >  8:314469/1‑148 (MQ=255)
                                                                tcGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTc                                                                                        >  7:1293984/1‑151 (MQ=255)
                                                                 cGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGAc                                                                                          <  3:860641/148‑1 (MQ=255)
                                                                   aTACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCAc                                                                                     <  2:720643/151‑1 (MQ=255)
                                                                    tACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAACTCGTCTCGCCACCCGCCCCTGCATTCAGCAAAATCAGCCTCCACg                                                                                    >  5:324576/1‑151 (MQ=255)
                                                                     aCAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACgg                                                                                   <  5:774792/151‑1 (MQ=255)
                                                                          caccaGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATg                                                                              >  3:330023/1‑151 (MQ=255)
                                                                           accaGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTccc                                                                                                               <  3:1299954/117‑1 (MQ=255)
                                                                           accaGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTccc                                                                                                               >  8:1293900/1‑117 (MQ=255)
                                                                             caGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTaa                                                                           <  4:607700/151‑1 (MQ=255)
                                                                                cGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAAttt                                                                        >  8:713031/1‑151 (MQ=255)
                                                                                 ggCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGGAAAATCAGACTCCACGGACATGTAAtttt                                                                       >  5:125981/1‑151 (MQ=255)
                                                                                 ggCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAAtttt                                                                       >  8:85893/1‑151 (MQ=255)
                                                                                  gCAGAAAATGATATTCCTGTCCTACAGTCTCTGCAATGCGCCTTTGTGTCTCGTCTGTAAGATAAAACTAATAAAGTGTCTGCCTGTGTAAATCCTCTCGCCACCCTACCCTGCATTCAGCAAAATCAGACTCCACGGACATTTAATTTTg                                                                      <  1:623373/151‑1 (MQ=255)
                                                                                     gTAAATGAAATTCCTGTCCGACAGGCGCTGCGATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATa                                                                   <  6:1142788/151‑1 (MQ=255)
                                                                                     gTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTGAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGAt                                                                    >  5:609923/1‑150 (MQ=255)
                                                                                     gTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATa                                                                   <  2:900610/151‑1 (MQ=255)
                                                                                       aaaTGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATaa                                                                  <  1:160879/150‑1 (MQ=255)
                                                                                             aaTTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCCCGGACATGTAATTTTGATAACGaaca                                                            >  4:1080569/1‑150 (MQ=255)
                                                                                              aTTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGaacaac                                                          >  8:757150/1‑151 (MQ=255)
                                                                                                tCCTGTCCGACATGCTCTGAAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTAATCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTTATAACGAACAACg                                                         <  8:808623/150‑1 (MQ=255)
                                                                                                tCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACg                                                         <  1:57695/150‑1 (MQ=255)
                                                                                                tCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGt                                                        >  3:655649/1‑151 (MQ=255)
                                                                                                      ccGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGGCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCt                                                  <  2:194384/151‑1 (MQ=255)
                                                                                                        gACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGCACAACGTTGACCttt                                                >  7:1223792/1‑151 (MQ=255)
                                                                                                          cAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGt                                              <  2:418346/151‑1 (MQ=255)
                                                                                                           aGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGtt                                             >  1:989557/1‑151 (MQ=255)
                                                                                                           aGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGtt                                             <  6:257323/151‑1 (MQ=255)
                                                                                                           aGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGtt                                             >  3:936361/1‑151 (MQ=255)
                                                                                                            ggCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGCCCTTTGtt                                             >  3:530881/1‑150 (MQ=255)
                                                                                                              cgcTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGGCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACa                                          <  8:174318/151‑1 (MQ=255)
                                                                                                              cgcTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGCCTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACa                                          >  7:671537/1‑150 (MQ=255)
                                                                                                               gcTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCACCAAAATCACACACCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACa                                          >  7:1354123/1‑150 (MQ=255)
                                                                                                                cTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAAtt                                       >  8:92676/1‑151 (MQ=255)
                                                                                                                  gCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTa                                      <  8:1230993/150‑1 (MQ=255)
                                                                                                                   cAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAg                                     <  7:1445260/151‑1 (MQ=255)
                                                                                                                    aaTGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGCCTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAAtt                                       >  2:286951/1‑148 (MQ=255)
                                                                                                                    aaTGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAAtt                                       >  1:1205615/1‑148 (MQ=255)
                                                                                                                     aTGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGAt                                   <  8:1393461/151‑1 (MQ=255)
                                                                                                                        ggcCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCa                                <  4:996157/150‑1 (MQ=255)
                                                                                                                          ccTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAAt                              <  6:983097/151‑1 (MQ=255)
                                                                                                                          ccTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAAt                              <  4:207951/151‑1 (MQ=255)
                                                                                                                          ccTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTAGTTACAATTAGATTCAAt                              >  1:723218/1‑151 (MQ=255)
                                                                                                                             gTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAAtt                             <  4:310319/149‑1 (MQ=255)
                                                                                                                               ttGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCCCCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGa                          >  1:303393/1‑150 (MQ=255)
                                                                                                                               ttGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGa                          >  7:1080145/1‑150 (MQ=255)
                                                                                                                                tGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAAt                        >  4:831987/1‑151 (MQ=255)
                                                                                                                                 gTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAAtt                       <  5:706363/151‑1 (MQ=255)
                                                                                                                                  tctcGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAAttt                      >  5:996297/1‑151 (MQ=255)
                                                                                                                                     cgtcgGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAAttt                      <  8:1060321/148‑1 (MQ=255)
                                                                                                                                     cgtcgGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTa                     <  3:1209508/149‑1 (MQ=255)
                                                                                                                                        cgGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGttt                >  7:850730/1‑151 (MQ=255)
                                                                                                                                          gTAAGCGAAAAGTAATATAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGATATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGttttt              <  4:886631/151‑1 (MQ=255)
                                                                                                                                          gTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATGCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGAGCTTTGTTACATTTTGATTCAATTTGAATTTATGttttt              <  7:545286/151‑1 (MQ=255)
                                                                                                                                            aaGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTg             <  3:2850/150‑1 (MQ=255)
                                                                                                                                            aaGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTg             >  6:80704/1‑150 (MQ=255)
                                                                                                                                            aaGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGa            <  3:1047123/151‑1 (MQ=255)
                                                                                                                                             aGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGaa           <  1:1410455/151‑1 (MQ=255)
                                                                                                                                                 aaaaGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGTCATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCtt      <  8:677484/151‑1 (MQ=255)
                                                                                                                                                     gTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTATTTGAATGCTTTCtt  >  3:1377135/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
ACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTTTCTT  >  NC_000913/3247336‑3247635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: