Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG29.8% 581.7 / 96.8 344intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (54/184);  new base G (98/3);  total (157/187)
Fisher's exact test for biased strand distribution p-value = 2.91e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGC  >  NC_000913/422279‑422578
                                                                                                                                                      |                                                                                                                                                     
  tttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcc                                                                                                                                                     >  8:140545/1‑151 (MQ=255)
   ttCGCTTGGATTGTTGTGCTGTTGATTGGCTGGATGGTTAAACGCCGCCACGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATGCTGATCCGGCTATTCCGAAATTTATCCGCCCCCGtcc                                                                                                                                                     <  4:601090/150‑1 (MQ=255)
     cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                  <  6:538446/151‑1 (MQ=255)
       cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGATGAAATCCTCATCCGGCTATTCCGAAATTTATCCGCGCGCGtccggcgc                                                                                                                                                >  8:6451/1‑146 (MQ=255)
        gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcctcctcc                                                                                                                                               >  2:656855/1‑151 (MQ=255)
           gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGccccc                                                                                                                                            >  6:927408/1‑151 (MQ=255)
             ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGAACTCCTACcaca                                                                                                                                           >  4:975966/1‑150 (MQ=255)
               gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTCCGCCCCCcaa                                                                                                                                         >  1:744912/1‑150 (MQ=255)
               gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCGCCAAat                                                                                                                                        >  5:410912/1‑151 (MQ=255)
                       gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGGTCGTCCCCCAAATACACTTct                                                                                                                                >  2:1161155/1‑150 (MQ=255)
                       gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCCTTc                                                                                                                                 >  1:59238/1‑150 (MQ=255)
                       gTTGATTGGCTGGATGTTTAAACGCCGCCACGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                                <  8:381581/151‑1 (MQ=255)
                        ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCAATATCCTTc                                                                                                                                 >  5:1257394/1‑149 (MQ=255)
                          gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCGTCCCCCCAATATCCttg                                                                                                                                 >  5:512801/1‑146 (MQ=255)
                            ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCACCCCACCTCCACCCCCAAATAACCATCAgatc                                                                                                                            >  1:1363608/1‑149 (MQ=255)
                             tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                           <  6:338274/150‑1 (MQ=255)
                             tGGCTGGATGTGTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                           <  5:1082930/150‑1 (MQ=255)
                              ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAACTATCCTTCAgatgatt                                                                                                                         >  6:1150826/1‑150 (MQ=255)
                               gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                        <  2:835489/151‑1 (MQ=255)
                                   gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAATACTTCAGAGGATGAGTg                                                                                                                     >  1:304536/1‑150 (MQ=255)
                                     tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCTCCGCCCCCCAATATCCTTCAGAGGAGGAGTGCTc                                                                                                                  >  6:1377122/1‑151 (MQ=255)
                                      gTTTAAACGCCGCCACGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                  <  6:409603/150‑1 (MQ=255)
                                         tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                               <  8:1281859/150‑1 (MQ=255)
                                         tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                               <  7:1425643/150‑1 (MQ=255)
                                            aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                           <  3:348898/151‑1 (MQ=255)
                                              gccgccACGCGCGTCAGATGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                         <  5:1361014/151‑1 (MQ=255)
                                              gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGCCGCGGCCCCCCCATATCCTGCAGTTGATGAGTGATcgggggtt                                                                                                           >  2:1245773/1‑142 (MQ=255)
                                               ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                        <  4:1247011/151‑1 (MQ=255)
                                               ccgccACGATCGTCAGCTGGCGGAAAACAAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                        <  6:298574/151‑1 (MQ=255)
                                                cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTGCTCGCCCAAATATCCTTCAGATGATGGGTGGTCCGGCAGTAGGc                                                                                                       >  8:836241/1‑151 (MQ=255)
                                                cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCCAATATCCGTCAGCTGATGAGTGAGCCTGCATTAggg                                                                                                       >  3:787680/1‑150 (MQ=255)
                                                 gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCt                                                                                                      <  1:820590/151‑1 (MQ=255)
                                                    aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                   <  2:1202431/151‑1 (MQ=255)
                                                    aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCCGCGCCCCCAAATATGCTTCAGATTATGAGTGAGGCTGGCTTACGCTATg                                                                                                   >  6:895549/1‑151 (MQ=255)
                                                    aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGGCCCCAAAGAACCTTCAGATCATGAGTGATCCTGCTTTAGGCTATg                                                                                                   >  6:1321755/1‑151 (MQ=255)
                                                    aCGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                   <  5:238751/151‑1 (MQ=255)
                                                     cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGCGGCCGCCGGCCGCGACGATCCGAGAGATGCTGAGTGATGCTGCATTAGGCGATgg                                                                                                  >  4:310579/1‑151 (MQ=255)
                                                      gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                 >  7:649040/1‑151 (MQ=255)
                                                          gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTACGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                             <  7:553567/151‑1 (MQ=255)
                                                           gcAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                            <  1:899283/150‑1 (MQ=255)
                                                                 ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                        <  7:375185/149‑1 (MQ=255)
                                                                  gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGACAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                     <  3:430269/151‑1 (MQ=255)
                                                                  gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                     <  2:7274/151‑1 (MQ=255)
                                                                   ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTAAGATGATGAGTGATCCTGCATTATGCGATGGCAAGGTGagg                                                                                        >  4:688061/1‑145 (MQ=255)
                                                                    tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCCCCCCAATATCGTTCAGATTGGGAGTGATGCGGGATTAGGGTATGGGAAGGGGATCAGAtt                                                                                   >  2:1430460/1‑151 (MQ=255)
                                                                     gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGGGGCCTGCGCCCCCAAATATCCGTCAGATGAAGAGTGCTCCTGCAGTAGGGTATGGGGAGGGGGTCAGAtgt                                                                                  >  3:1197387/1‑149 (MQ=255)
                                                                          ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCGCCCCCCAATATCCTTCAGATGATGAGTGATGCTGCATTAGGGTATGGGAAGGTGATCAGATTTtcatc                                                                             >  8:766098/1‑151 (MQ=255)
                                                                           cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGCCGCCTCCCCCAACTATCCTTCAGAGGAGGAGTGCTCCTGCAATAGGATATGGCACGGTGATCAGATTttcttc                                                                             >  5:677656/1‑150 (MQ=255)
                                                                            aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTGCGCGCCCACAGATCCTTCAGATGCTGCGTGATGCTGGATTAGGGGATGGCAAGGGGTTCAGATTTtgatccc                                                                           >  6:121353/1‑149 (MQ=255)
                                                                               aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCTCGTCCCCCAAATAAACTTCAGAGGAAGAGTGATCCGGCATTAGGAGATGGGAAGGTGATCAGATTTTGATAAGAgg                                                                        >  3:941984/1‑151 (MQ=255)
                                                                               aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                         <  6:1474423/150‑1 (MQ=255)
                                                                                aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                       <  5:870379/151‑1 (MQ=255)
                                                                                  ccTTGCGTTCTGTACGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                      <  4:1040074/150‑1 (MQ=255)
                                                                                  ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                      <  5:1406793/150‑1 (MQ=255)
                                                                                   cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCAAAAATCCGTCAGATGATGCGTGATGCTGCCTGTGGGTGTGGCTAGGTGATCAGCTTTTGATCACAGGGGat                                                                    >  8:138013/1‑150 (MQ=255)
                                                                                   cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCCTTAGGATGTTGAGTGGTGCGGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGa                                                                     >  6:646357/1‑150 (MQ=255)
                                                                                      gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                                  <  7:324239/150‑1 (MQ=255)
                                                                                        gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGATTGATCCTGGATTAGGGTATGGCAAGGTGGTCAGATTTTGATCTCAGGGGAATTatg                                                               >  7:493465/1‑151 (MQ=255)
                                                                                        gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg                                                               <  6:724307/151‑1 (MQ=255)
                                                                                          tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCTCCCCCAAATATACTGGAGAGGATGGGTGTTCCTGCATTAGGCTATGGGGAGGTGATCAGCTTTTGATCACAGGGGAATTatgtt                                                             >  5:890368/1‑149 (MQ=255)
                                                                                           cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGAGCAGAGTTTAATCACAGGGGCATTatgatt                                                            >  1:351007/1‑150 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                             <  4:832441/148‑1 (MQ=255)
                                                                                             gTAAGCAGGTTCAGGAGGAAATCCTGATCCTGCTATTCCGAAAGTTATCCGGCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACATGGGAATTATGATGt                                                           <  2:1069017/150‑1 (MQ=255)
                                                                                              tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGGGCCGCCCCCACAGAGCCGTCAGAGGATGAGTGAGCCTGCATTAGGCGAGGGGAAGGGGATGAGATTTTGATGACAGGGGAGTTTGGATGttt                                                         >  4:55490/1‑150 (MQ=255)
                                                                                                aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCAAAGGGGATTTATGAttttta                                                        >  3:61866/1‑148 (MQ=255)
                                                                                                 gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                      <  8:1178154/151‑1 (MQ=255)
                                                                                                  ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                     <  8:1338697/151‑1 (MQ=255)
                                                                                                   cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCAGTAGGGTATGGCAAGGGGATCAGCTTTTCATCACAGGGGAATTATGATGTTAAATg                                                     >  2:1214455/1‑150 (MQ=255)
                                                                                                        cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCGATGGGAAGGTGATCAGATTTTAATCACAGGGGAATTATTATGTTTAATTAATgg                                                >  2:947944/1‑150 (MQ=255)
                                                                                                         aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCGCCCCCCAATATCCTTACGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTACTCACAGGGGAATTATGATGTTAAATTCATGGc                                               >  2:546277/1‑150 (MQ=255)
                                                                                                            aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                           <  5:530725/151‑1 (MQ=255)
                                                                                                            aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCACCCCCAAAAATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACCGGGGAAGTATGATGTTAAATGCATGGCCCCt                                           >  7:932954/1‑151 (MQ=255)
                                                                                                            aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATACTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCAAAGGGGATTTATGATGTTATATGCaatgctccg                                           >  1:655287/1‑143 (MQ=255)
                                                                                                             ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTGAGATGATGAGTGATGCTGCATTAGGGTATGGCAAGGTGATCAGATTTTAATCACAGGGGTATTATGATTTTAAAAGCATGGCACCTg                                          >  6:1114385/1‑151 (MQ=255)
                                                                                                               aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                        <  4:1352381/151‑1 (MQ=255)
                                                                                                               aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGGCCCCCAAAAAAACTTCAGAAGATAACTGATCATGCAGTAGGGTATGGCAAGATTATAAGAATTTAAAAACAGGGTAATAAATATATTAAATGAAttgaaactga                                         >  4:40423/1‑141 (MQ=255)
                                                                                                                aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCCAATAACCTTCAGATGATGAGTGAGCCTGCATTAGGCGATGGGAAGGTGAGCAGCTTTTAATCACAGGGGAATTATGATTTTAAATGAATGGCACCTGCCg                                       >  8:151968/1‑151 (MQ=255)
                                                                                                                   ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                    <  7:246536/151‑1 (MQ=255)
                                                                                                                      gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTGTGGCAAGGTGGTGAGATTTTGATCACAGGGGAATTATGATGTTAAATGCCTGGCGCCTGCCGGGGccc                                 >  7:105590/1‑151 (MQ=255)
                                                                                                                       aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcccc                                <  2:537425/151‑1 (MQ=255)
                                                                                                                            gCTATTCCGAACGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCTAGGGCAAGGGGAGCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                           <  4:228423/151‑1 (MQ=255)
                                                                                                                            gCTATTCCGAAAGGTATCCGCCCCCGTCCTCCTCCCCCAAATATCATTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                           <  7:824167/151‑1 (MQ=255)
                                                                                                                             cTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAACTATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGATTTTCATCACAGCGGAAGTATTATGTTAAATTAATGTCACCTGCCGGTGCCCCCATATg                          >  8:242604/1‑151 (MQ=255)
                                                                                                                              tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTAATAAGATGAGTGATCCTGCATTAGTCAATGGGAAGGTGATCAGATTTTGATAACATGGGAATTATGATGTTAAATAAATGGCACCTCACCGAGCCGCCAGTTGt                         >  1:1035827/1‑151 (MQ=255)
                                                                                                                               aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                          <  1:411521/149‑1 (MQ=255)
                                                                                                                               aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                         <  5:767210/150‑1 (MQ=255)
                                                                                                                                   cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                    <  2:994419/151‑1 (MQ=255)
                                                                                                                                   cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                    <  2:131723/151‑1 (MQ=255)
                                                                                                                                      aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                 <  6:1241542/151‑1 (MQ=255)
                                                                                                                                        gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc               <  8:497580/151‑1 (MQ=255)
                                                                                                                                         ttATCCGCCCCCGGCCTCCTCCCCCCAATATACGTCAGATGATGAGTGATCGTGCATTAGGCTATGGGAAGGGGATCAGATTTTCATCACAGGGGAATTATTATGTTTAATGAAAGCCCCCGGCCGGTGCCCCCATATGTTAAAaaaagc               >  3:516178/1‑150 (MQ=255)
                                                                                                                                          tATCCGCCCCCCGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGTCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGAATGGCACCAGCCGGTGCCCCCAATTGTTAAAaaaagca              >  3:109227/1‑150 (MQ=255)
                                                                                                                                           aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa            <  6:1043718/151‑1 (MQ=255)
                                                                                                                                           aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa             <  6:785551/150‑1 (MQ=255)
                                                                                                                                             ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag           <  2:194542/150‑1 (MQ=255)
                                                                                                                                             ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa          <  8:607089/151‑1 (MQ=255)
                                                                                                                                              cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt         <  2:286898/151‑1 (MQ=255)
                                                                                                                                                cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa       <  8:87589/151‑1 (MQ=255)
                                                                                                                                                   ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt    >  3:276212/1‑151 (MQ=255)
                                                                                                                                                     gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc  <  1:994923/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGC  >  NC_000913/422279‑422578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑